ABO antigens are not only present on the surface of red blood cells but are also secreted in body fluids. Individuals who can secrete these antigens in body fluids are called secretors while those who cannot are known as non-secretors. As compared to secretor non-secretors are at high risk to different bacterial, heart and autoimmune diseases but it is not clear that secretor status has an association with Helicobacter pylori. Therefore, this study was conducted to determine the secretor and non-secretor status in Helicobacter pylori infected patients. This cross-sectional study was carried out at Institute of Paramedical Sciences, Khyber Medical University Peshawar. A total of 192 samples were included in this study, 96 from Helicobacter pylori infected patients and 96 as a control group. Saliva samples were taken for the determination of secretor and non-secretor status by using Absorption inhibition method. Blood samples were obtained for ABO blood grouping and Helicobacter pylori determination. Out of 96 Helicobacter pylori infected patients, 36 (37.5%) were found as secretors and 60 (62.5%) were found as non-secretors while in normal individuals, secretors were 67 (69.8%) and non-secretors were 29 (30.2%). In H. pylori infected patients, classification of various ABH blood groups like A, B, AB and O were 24 (25%), 33 (34.3%), 18 (18.8%) and 21 (21.9%) respectively. In normal individuals’ the frequency of ABO was 28 (29.2%) of A, 28 (29.2%) of B, 8 (8.3%) of AB and 32 (33.3%) of O blood groups respectively. It is concluded that Helicobacter pylori infected patients have lowest secretor and highest non-secretors, because ABH antigens present in the body fluids of secretor show resistance to Helicobacter pylori infection. Moreover, non-secretors are more susceptible to bacterial infections. And it is known that Helicobacter pylori is a bacterium which can cause bacterial infections like gastric ulcer and duodenal ulcer. While normal individuals have highest secretor and lowest non-secretor status. Keywords: ABO blood groups; Body fluids; Helicobacter pylori; Non-secretor http://dx.doi.org/10.19045/bspab.2021.100053