Aberrant Tissue Research Articles

Bilateral pulmonary sequestrations: Distinct types sharing a common celiac artery origin, presented with pneumonia and associated findings in a pediatric case.

A 14-year-old boy presented with shortness of breath, cough, and mild chest pain, with a history of intermittent milder symptoms. Physical examination and initial tests showed mild iron deficiency anemia, elevated C-reactive protein, and normal vital signs. Chest radiograph revealed abnormalities in the left lower zone, leading to contrast-enhanced chest CT. The CT scan revealed bilateral intrapulmonary sequestrations, both deriving blood supply from a common trunk originating from the celiac artery. The patient's symptoms initially attributed to a pulmonary infection improved with antibiotic therapy. Pulmonary sequestration is a congenital anomaly characterized by aberrant lung tissue lacking connections to bronchial tree or pulmonary arteries. It can lead to recurrent pulmonary infections and postinfectious sequelae. This case presented a unique bilateral sequestration, both originating from the celiac artery. Radiologists should be aware of sequestration types and associated anomalies, even in atypical locations. Blood supply can originate from various arteries, not just the aorta.

Microvascular Endothelial Glycocalyx Surface Layer Visualization and Quantification.

As a primary interface between the blood and underlying vascular wall, the endothelial glycocalyx layer is common to all blood vessels and covers the luminal surface of all endothelial cells. The endothelial glycocalyx has important roles as a regulator of microvascular endothelial functions such as mechanotransduction, leukocyte adhesion, and microvascular permeability. Disruption of the molecular structure of the endothelial glycocalyx disturbs physiological, and hemodynamic processes associated with the microvascular wall leads to microvascular hyperpermeability. Studying the glycocalyx is challenging because cultured cells present aberrant glycocalyx structure and tissue fixation techniques lead to the degradation and loss of this fine and delicate layer. Therefore, studying the glycocalyx requires in vivo imaging of the microcirculation. Here we describe two techniques for direct imaging and assessment of the glycocalyx surface layer integrity using intravital microscopy (IVM), a method widely used in the study of the dynamic changes that occur in the microcirculation during inflammation or injury.

Normal and Aberrant Muscle Tissue Healing, Learning from Health and Disease

Human skeletal muscle exhibits remarkable plasticity, being responsive to chemical, mechanical, metabolic, and inflammatory stress. When the homeostatic disturbance is below a threshold of significant damage, the muscle responds by modifying metabolic activity, cell size/shape, and structure, thereby normalizing cellular function. If the disturbance causes significant damage, skeletal muscle, along with a precisely choreographed response from the immune system, can regenerate. Very few pathological conditions inhibit these adaptive responses in muscle. Yet, from these few conditions, we can learn a great deal. Working with the immune system, normal muscle healing can inform disease treatments, and the disease pathology informs our understanding of normal muscle healing. Here we use Duchenne Muscular Dystrophy (DMD) as a model of failed muscle adaptation/regeneration to attempt to understand normal muscle healing, why it sometimes fails, and how normal muscle response might be applied to understand and treat DMD.

Brain Pathology Classification of MR Images Using Machine Learning Techniques

A brain tumor is essentially a collection of aberrant tissues, so it is crucial to classify tumors of the brain using MRI before beginning therapy. Tumor segmentation and classification from brain MRI scans using machine learning techniques are widely recognized as challenging and important tasks. The potential applications of machine learning in diagnostics, preoperative planning, and postoperative evaluations are substantial. Accurate determination of the tumor’s location on a brain MRI is of paramount importance. The advancement of precise machine learning classifiers and other technologies will enable doctors to detect malignancies without requiring invasive procedures on patients. Pre-processing, skull stripping, and tumor segmentation are the steps involved in detecting a brain tumor and measurement (size and form). After a certain period, CNN models get overfitted because of the large number of training images used to train them. That is why this study uses deep CNN to transfer learning. CNN-based Relu architecture and SVM with fused retrieved features via HOG and LPB are used to classify brain MRI tumors (glioma or meningioma). The method’s efficacy is measured in terms of precision, recall, F-measure, and accuracy. This study showed that the accuracy of the SVM with combined LBP with HOG is 97%, and the deep CNN is 98%.

Epithelial-Mesenchymal Transition Mechanisms in Chronic Airway Diseases: A Common Process to Target?

Epithelial-to-mesenchymal transition (EMT) is a reversible process, in which epithelial cells lose their epithelial traits and acquire a mesenchymal phenotype. This transformation has been described in different lung diseases, such as lung cancer, interstitial lung diseases, asthma, chronic obstructive pulmonary disease and other muco-obstructive lung diseases, such as cystic fibrosis and non-cystic fibrosis bronchiectasis. The exaggerated chronic inflammation typical of these pulmonary diseases can induce molecular reprogramming with subsequent self-sustaining aberrant and excessive profibrotic tissue repair. Over time this process leads to structural changes with progressive organ dysfunction and lung function impairment. Although having common signalling pathways, specific triggers and regulation mechanisms might be present in each disease. This review aims to describe the various mechanisms associated with fibrotic changes and airway remodelling involved in chronic airway diseases. Having better knowledge of the mechanisms underlying the EMT process may help us to identify specific targets and thus lead to the development of novel therapeutic strategies to prevent or limit the onset of irreversible structural changes.

A Diagnostic Study of Content-Based Image Retrieval Technique for Studying the CT Images of Lung Nodules and Prediction of Lung Cancer as a Biometric Tool

Content Based Medical Image Retrieval (CBMIR) can be defined as a digital image search using the contents of the images. CBMIR plays a very important part in medical applications such as retrieving CT images and more accurately diagnosing aberrant lung tissues in CT images. The Content-Based Medical Image Retrieval (CBMIR) method might aid radiotherapists in examining a patient's CT image in order to retrieve comparable pulmonary nodes more precisely by utilizing query nodes. Intending a particular query node, the CBMIR system searches a large chest CT image database for comparable nodes. The prime aim of this research is to evaluate an end-to-end method for developing a CBIR system for lung cancer diagnosis.

Naïve Bayesian Classification Based Glioma Brain Tumor Segmentation Using Grey Level Co-occurrence Matrix Method

Brain tumors vary widely in size and form, making detection and diagnosis difficult. This study's main aim is to identify abnormal brain images., classify them from normal brain images, and then segment the tumor areas from the categorised brain images. In this study, we offer a technique based on the Nave Bayesian classification approach that can efficiently identify and segment brain tumors. Noises are identified and filtered out during the preprocessing phase of tumor identification. After preprocessing the brain image, GLCM and probabilistic properties are extracted. Naive Bayesian classifier is then used to train and label the retrieved features. When the tumors in a brain picture have been categorised, the watershed segmentation approach is used to isolate the tumors. This paper's brain pictures are from the BRATS 2015 data collection. The suggested approach has a classification rate of 99.2% for MR pictures of normal brain tissue and a rate of 97.3% for MR images of aberrant Glioma brain tissue. In this study, we provide a strategy for detecting and segmenting tumors that has a 97.54% Probability of Detection (POD), a 92.18% Probability of False Detection (POFD), a 98.17% Critical Success Index (CSI), and a 98.55% Percentage of Corrects (PC). The recommended Glioma brain tumour detection technique outperforms existing state-of-the-art approaches in POD, POFD, CSI, and PC because it can identify tumour locations in abnormal brain images.

Mechanisms of aberrant tissue residency programming of uterine natural killer cells in uterus transplantation

Abstract Uterine natural killer cells (uNK) are the most common human decidual lymphocytes, but the origin and ontogeny of uNKs remain unclear. Studies of human uterus transplant recipients (UTx) suggest that uNKs originate in the blood, but how these cells become tissue resident and differentiate into decidual NK cells is unknown. We studied the molecular mechanisms governing uNK tissue residency in human endometrium using single-cell RNA-seq and flow cytometry analyses. trNK cells with a CD49a+ CD16− phenotype were the most abundant CD56+ cells in the endometrium of 5 healthy control volunteers (HCs) [27±17% trNK vs. 4±2.7% CD49a− CD16+ conventional NK cells (cNK); p<0.02]. trNK cells differentially expressed genes associated with T cell tissue residency, such as ITGAE and ZNF683, compared to cNK. Although peripheral blood NK cells can express CD103 when incubated with TGF-β, trNKs upregulated CD103 expression in response to IL-15 alone in vitro. Given the dependency of CD103 expression on NFAT signals, we studied CD103 expression on trNKs by incubating human endometrium (n=7) with the calcineurin inhibitor FK506, which decreased IL-15-induced upregulation of CD103 (81% CD103+ [IL-15 alone] vs. 70% CD103− [IL-15 + FK506]; p=0.02). We next tested the in vivo relevance of these findings by comparing the frequency of trNKs in endometrial biopsies of five HCs with two biopsies of one UTx on FK506 immunosuppression. The UTx recipient had significantly fewer trNKs among CD45+ cells in two biopsies (HC: 27±17% vs. UTx: 4.3±2%; p=0.04). Altogether these findings suggest that IL-15-induced calcium signals influence uNK tissue residency, with significant implications for transplant recipients. This work was supported from UAB AMC21 and support of the uterus transplant program from the University of Alabama at Birmingham. P. Porrett is additionally supported by NIH R01 AI145905.

Aberrant serum and tissue levels of Beclin1 and mechanistic target of rapamycin (mTOR) proteins in epithelial ovarian cancer.

Beclin1 and mechanistic target of rapamycin (mTOR) can be used as tumor markers of epithelial ovarian cancer. This study aimed to assess the association of Beclin1 and mTOR expression with clinicopathological and prognostic data in epithelial ovarian cancer patients. Serum and tissue samples from 45 epithelial ovarian cancer patients and 20 controls were analyzed by enzyme-linked immunosorbent assay and immunohistochemistry for Beclin1 and mTOR expression. The online datasets from gene expression profiling interactive analysis (n = 426), Kaplan-Meier plotter (n = 398), cBioPortal (n = 585), and UALCAN (n = 302) were also analyzed. Beclin1 expression was associated with low-grade differentiation (P = .003), earlier clinical stage (P = .013), fewer local lymph node metastases (P = .02) and lower serum Beclin1 level (P = .001). mTOR expression was associated with high-grade differentiation (P = .013), advanced clinical stage (P = .021), ascites (P = .028), and higher serum mTOR level (P = .001). The online datasets showed that a high mTOR expression level (HR = 1.44; 95% CI = 1.08-1.92; P = .013) was associated with a poor overall survival of 426 patients. Beclin1 was mutated in 1.8% and mTOR was mutated in 5% of epithelial ovarian cancer patients. Serum Beclin1 and mTOR levels were able to predict tumor differentiation, clinical stage, lymph node metastasis, and ascites in epithelial ovarian cancer patients.

Segmentation of Brain Tumor using Multiple Threshold Technique

Radiology use medical imaging techniques to comprehend the structure and physiological functions of the body in bothhealthy and diseased subjects. A non-invasive method for viewing internal body structures can be performed by using magneticresonance imaging (MRI). MRI characterizes soft tissue more accurately than other imaging methods like CT. In the currentstudy, space-occupying lesions are visualized using MRI imaging. Slices of MRI data are used to analyze lesions. Single sliceanalysis is inappropriate to determine the lesion’s size and volume. Hence, the MRI sequence is used to segment the lesions.Following segmentation, we view the MRI 2D image in 3D to look for lesions, or aberrant tissue, in the brain. The lesion isthen visualized by performing clipping. This research suggests segmenting brain tumors automatically and even provides a3D visualization for a more thorough study. Here, a space-occupying lesion is segmented from a T2 weighted Flair sequenceof MR images in DICOM format, and by employing the segmented volume, 3D rendering and clipping are made possible

LC-QToF-Based Metabolomics Identifies Aberrant Tissue Metabolites Associated with a Higher-Fat Diet and Their 'Reversion to Healthy' with Dietary Probiotic Supplementation.

Over 33% of Americans are labeled as obese, leading the World Health Organization to designate obesity as a major public health problem. One consequence of obesity is the development of metabolic syndrome, a condition which has been correlated to an increased risk for developing cardiovascular disease and Type 2 diabetes. Prolonged ingestion of a higher-fat diet, one cause of obesity, results in alterations to the gut microbiome. These alterations are implicated to have a profound role in the evolution and progression of obesity-linked diseases. Probiotics are associated with positive health effects such as limiting pathogen colonization, aiding in digestion, and vitamin synthesis. Using Ossabaw pigs as a model for obesity, and in conjunction with our previous research, we performed an in-depth, nontargeted, metabolomic analysis on select organs to elucidate the effects of dietary supplementation with the probiotic Lacticaseibacillus paracasei. We focused our analysis on the effects of probiotic supplementation on a higher-fat (obesogenic) diet and a nutritionally balanced diet. Notably, our findings reveal that the brain cortex is highly sensitive to dietary influencers, and with probiotic supplementation, several aberrant metabolites associated with a higher-fat diet revert to healthy levels, thus demonstrating the potential for a probiotic intervention for obesity-linked disease.

Loop electrosurgical excision procedure: A Review

LEEP is a one-of-a-kind outpatient procedure for diagnosing and treating dysplastic cervical lesions. Loop diathermy treatment, loop excision of the transformation zone (LETZ), and large loop excision of the transformation zone (LLETZ) are other names. This approach yields high-quality cytologic specimens and has no negative effects on reproductive function. It also can be used to treat cervical cancer by replacing cryotherapy or laser. LEEP can be used to bypass the entire transformation zone. This is done with the help of a high-frequency, low-current electric generator and a stainless-steel loop. The LEEP procedure has a similar complication rate to cryotherapy. Bleeding is the most common complication. In addition, there may be inadequate lesion removal and cervical stenosis. LEEP has several advantages over other treatments, including removing aberrant tissue that allows cytologic examination, cheap cost, simplicity of acquiring important skills, and the potential to treat lesions with fewer visits. Patients are pleased with the surgery. LEEP is anticipated to be widely used by family physicians.

Genome-wide liver transcriptomic profiling of a malaria mouse model reveals disturbed immune and metabolic responses

BackgroundThe liver is responsible for a range of functions in vertebrates, such as metabolism and immunity. In malaria, the liver plays a crucial role in the interaction between the parasite and host. Although malarial hepatitis is a common clinical complication of severe malaria, other malaria-related liver changes have been overlooked during the blood stage of the parasite life-cycle, in contrast to the many studies that have focused on parasite invasion of and replication in the liver during the hepatic stage of the parasite.MethodsA rodent model of malaria was established using Plasmodium yoelii strain 17XL, a lethal strain of rodent malaria, for liver transcriptomic profiling.ResultsDifferentially expressed messenger RNAs were associated with innate and adaptive immune responses, while differentially expressed long noncoding RNAs were enriched in the regulation of metabolism-related pathways, such as lipid metabolism. The coexpression network showed that host genes were related to cellular transport and tissue remodeling. Hub gene analysis of P. yoelii indicated that ubiquitination genes that were coexpressed with the host were evolutionarily conserved.ConclusionsOur analysis yielded evidence of activated immune responses, aberrant metabolic processes and tissue remodeling changes in the livers of mice with malaria during the blood stage of the parasite, which provided a systematic outline of liver responses during Plasmodium infection.Graphical

Mechanic Insight into the Distinct and Common Roles of Ovariectomy Versus Adrenalectomy on Adipose Tissue Remodeling in Female Mice.

Dysfunctions of the ovaries and adrenal glands are both evidenced to cause aberrant adipose tissue (AT) remodeling and resultant metabolic disorders, but their distinct and common roles are poorly understood. In this study, through biochemical, histological and RNA-seq analyses, we comprehensively explored the mechanisms underpinning subcutaneous (SAT) and visceral adipose tissue (VAT) remodeling, in response to ovariectomy (OVX) versus adrenalectomy (ADX) in female mice. OVX promoted adipocyte differentiation and fat accumulation in both SAT and VAT, by potentiating the Pparg signaling, while ADX universally prevented the cell proliferation and extracellular matrix organization in both SAT and VAT, likely by inactivating the Nr3c1 signaling, thus causing lipoatrophy in females. ADX, but not OVX, exerted great effects on the intrinsic difference between SAT and VAT. Specifically, ADX reversed a large cluster of genes differentially expressed between SAT and VAT, by activating 12 key transcription factors, and thereby caused senescent cell accumulation, massive B cell infiltration and the development of selective inflammatory response in SAT. Commonly, both OVX and ADX enhance circadian rhythmicity in VAT, and impair cell proliferation, neurogenesis, tissue morphogenesis, as well as extracellular matrix organization in SAT, thus causing dysfunction of adipose tissues and concomitant metabolic disorders.

Hepatoprotective Efficacy of Cycloastragenol Alleviated the Progression of Liver Fibrosis in Carbon-Tetrachloride-Treated Mice.

The continuous death of hepatocytes induced by various etiologies leads to an aberrant tissue healing process and promotes the progression of liver fibrosis and ultimately chronic liver diseases. To date, effective treatments to delay this harmful process remain an unmet clinical need. Cycloastragenol is an active phytochemical substance isolated from Astragalus membranaceus, a plant used in traditional Chinese medicine to protect the liver. Therefore, our study aimed to elucidate the efficacy of cycloastragenol on carbon-tetrachloride (CCl4)-induced liver fibrosis in mice. We found that cycloastragenol at 200 mg/kg dosage exhibited anti-fibrotic efficacy as demonstrated by a decrease in collagen deposition, downregulation of mRNA expression of collagen type 1, and a reduction in the content of total collagens. In addition, cycloastragenol further augmented the levels of anti-fibrotic matrix metalloproteinases (Mmps), that is, Mmp8, proMmp9, and Mmp12, which play a pivotal role in fibrosis resolution. According to histological analysis and serum markers of hepatotoxicity, cycloastragenol protected the livers from damage and mitigated the increment of serum alanine aminotransferase and bilirubin implicating hepatoprotective efficacy against CCl4. Moreover, cycloastragenol upregulated the mRNA expression of interleukin 6, a pleiotropic cytokine plays a vital role in the promotion of hepatocyte regeneration. In conclusion, cycloastragenol alleviated the progression of liver fibrosis in CCl4-treated mice and its anti-fibrotic efficacy was mainly due to the hepatoprotective efficacy.

A RARE CASE OF INTRAOSSEOUS MUCOEPIDERMOID CARCINOMA OF MAXILLA TREATED AT OUR CENTRE.

Occurrence of Primary intraosseous mucoepidermoid carcinoma (PIOC) within the jaw bones from aberrant salivary tissues is extremely rare. Mandible is more commonly affected but occurrence in maxilla is very rare. The origin of mucoepidermoid carcinoma is controversial and debatable. Primary Intraosseous mucoepidermoid carcinoma is frequently misdiagnosed radiographically and clinically as a benign odontogenic tumor or cyst.Primary Intraosseous Mucoepidermoid Carcinoma has distinct and specic radiological and morphological features for its diagnosis. The main modality of treatment is surgery and appropriate patients are offered adjuvant radiotherapy. This paper reports a case of central mucoepidermoid carcinoma of the Maxilla which was presented as complaints of pain and swelling of upper jaw.Then, he was treated with surgery followed by adjuvant treatment as per specimen pathological report. Here, we have used Simultaneous Integrated Boost technique with helical IMRT for Radiotherapy along with concurrent chemotherapy for treatment of revised margins following positive margins status. Central mucoepider Summary: moid carcinoma is a rare entity with highly selective radiological, histological and immunohistochemical criteria with overall around 150 cases reported. Due to rarity of occurrence, consensus guidelines for treatment protocol have not been established. Here we have described a similar case of a 25yrs old male patient that was diagnosed, surgically resected and has completed adjuvant chemo-radiation therapy at our centre

Aberrant pancreas adenocarcinoma in the stomach: A case report and literature review.

Aberrant pancreatic tissue in the gastrointestinal tract is a relatively common finding. However, malignant transformation is extremely rare. Herein, we report a case of ectopic pancreatic ductal adenocarcinoma in the stomach wall. A 38 year old male presented with nausea, bloating, abdominal distention and weight loss for 4 months. Endoscopy of upper gastrointestinal tract was performed twice with 2 months interval and a stenotic pyloric part was observed with a suspected submucosal lesion. It was sampled both times, however the pathology findings of the mucosal biopsies were unremarkable with no identifiable neoplastic structures. CT scan and MRI was performed and showed a thickened pyloric wall with a submucosal lesion 15 × 15 mm in diameter. Blood levels of tumor markers carcinoembrionic antigen and carbohydrate antigen 19-9 were within a normal range. Pyloric stenosis progressed and the patient underwent a Billroth type I distal gastric resection with D2 lymphadenectomy. Pathologic examination revealed a well differentiated ductal adenocarcinoma arising in the heterotopic pancreatic tissue (Heinrich type III). The resection margins and lymph nodes were free of tumor. The patient received adjuvant chemotherapy with 6 courses of XELOX. No disease recurrence is reported in 12 months follow-up. Aberrant pancreatic ductal adenocarcinoma in the stomach is a rare finding, however this pathology should be included in the differential diagnosis of gastric submucosal lesion causing pyloric stenosis.

Opto-electromechanical quantification of epithelial barrier function in injured and healthy airway tissues.

The airway epithelium lining the luminal surface of the respiratory tract creates a protective barrier that ensures maintenance of tissue homeostasis and prevention of respiratory diseases. The airway epithelium, unfortunately, is frequently injured by inhaled toxic materials, trauma, or medical procedures. Substantial or repeated airway epithelial injury can lead to dysregulated intrinsic repair pathways and aberrant tissue remodeling that can lead to dysfunctional airway epithelium. While disruption in the epithelial integrity is directly linked to degraded epithelial barrier function, the correlation between the structure and function of the airway epithelium remains elusive. In this study, we quantified the impact of acutely induced airway epithelium injury on disruption of the epithelial barrier functions. By monitoring alternation of the flow motions and tissue bioimpedance at local injury site, degradation of the epithelial functions, including mucociliary clearance and tight/adherens junction formation, were accurately determined with a high spatiotemporal resolution. Computational models that can simulate and predict the disruption of the mucociliary flow and airway tissue bioimpedance have been generated to assist interpretation of the experimental results. Collectively, findings of this study advance our knowledge of the structure-function relationships of the airway epithelium that can promote development of efficient and accurate diagnosis of airway tissue injury.

Congenital pseudoarthrosis of the tibia and fibula is managed with plate osteosynthesis and the Ilizarov method

Pseudo means false, and arthrosis means joint formation. Congenital pseudoarthrosis (C.P.T.) of the tibia is a rare congenital anomaly. The incidence of it is one in 190,000 live births. Although rare, C.P.T. of the tibia poses one of the most challenging management problems in pediatric orthopaedics. It is characterized by de-ossification, bending, and pathological fracture, followed by the inability to form a normal callus at the fracture site. Fifty-five percent of cases of pseudoarthrosis are associated with neurofibromatosis-1 (NF-1), and 6% of patients with NF-1 develop pseudoarthrosis of the tibia. A 9-year-old male came to the orthopaedic outpatient department with a deformity over his right leg since birth. There were multiple café au lait spots on the front and back of the trunk. The right leg had a bony deformity in the lower third with anterior convexity, with typical features of pseudarthrosis tibia and fibula, along with gross abnormal mobility and 2 cm shortening. The left leg was normal. There is no evidence of any new bone formation at the non-union sites. The patient was managed with refreshing the bone ends to produce bleeding bone ends with good contact and open removal of aberrant fibrous tissue from the region of the tibia´s pseudoarthrosis. Bone fragments were reduced and fixed with a locking plate. Corticotomy was done at the proximal end of the tibia to improve the limb length discrepancy via the distraction osteogenesis method, and an Ilizarov ring fixator was applied. Postoperatively, distraction was done at 1 mm per day.

Chronic unpredictable mild stress promotes atherosclerosis via adipose tissue dysfunction in ApoE-/- mice.

Chronic unpredictable mild stress (CUMS) has been shown to exacerbate atherosclerosis, but the underlying mechanism remains unknown. Adipose tissue is an energy storage organ and the largest endocrine organ in the human body, playing a key role in the development of cardiovascular disease. In this research, it was hypothesized that CUMS may exacerbate the development of atherosclerosis by inducing the hypertrophy and dysfunction of white adipocytes. The CUMS-induced atherosclerosis model was developed in Western diet-fed apolipoprotein E (ApoE)-/- mice. White adipose tissue (WAT), serum, aortic root, and the brachiocephalic trunk were collected and tested after 12 weeks of CUMS development. The mouse model of CUMS was evaluated for depression-like behavior using the open field test (OFT) and the elevated plus maze (EPM) test. Enzyme-linked immunosorbent assay (ELISA) was conducted to detect serum noradrenaline and urine adrenaline protein levels. Serological assays were used to detect serum low-density lipoprotein (LDL), high-density lipoprotein (HDL), total cholesterol (TC), and free fatty acid (FFA) concentrations. Hematoxylin and eosin (H&E) staining and oil red O were used to detect atherosclerotic plaque area, lipid deposition, and adipocyte size. The mRNA levels of genes related to aberrant adipose tissue function were determined using real-time PCR. Immunofluorescence assay and western blotting were conducted to examine the expression of proteins in the adipose tissue samples. CUMS aggravated vascular atherosclerotic lesions in ApoE-/- mice. It decreased body weight while increasing the percentage of WAT. The serological results indicated that the concentration of HDL decreased in CUMS mice. Notably, adipocyte hypertrophy increased, whereas the mRNA levels of Pparg and its target genes (Slc2a4 (encodes for GLUT4), Adipoq, and Plin1) decreased. Further investigation revealed that CUMS increased subcutaneous inguinal WAT (iWAT) lipid synthesis and adipocyte inflammation while decreasing lipid hydrolysis and the expression of HDL-associated protein ApoA-I. Moreover, CUMS aggravated insulin resistance in mice and inhibited the insulin pathway in iWAT. These findings indicated that CUMS induces adipose tissue dysfunction via a mechanism that leads to dyslipidemia, increased inflammation, and insulin resistance in the body, thereby exacerbating atherosclerosis. Notably, CUMS that is involved in decreasing the expression of HDL-associated proteins in adipose tissue may be a crucial link between adipose hypertrophy and advanced atherosclerosis. This study reveals a novel mechanism via which CUMS exacerbates atherosclerosis from the novel perspective of abnormal adipose function and identifies a novel potential therapeutic target for this disease.