Background and ObjectivesDyslipidemia is one of the major predictors of cardiovascular disease. Cardiovascular diseases are the major cause of morbidity and mortality in developed as well as developing countries around the world. Cardiovascular diseases are becoming more prevalent in Pakistan and are the major cause of mortality in Pakistan. The number of studies previously carried out on ABO blood group association has been reported with many diseases including cancer, ulcer, diabetes, and hypertension. However, no study has previously reported an association of blood groups in apparently normal subjects. The objective of the study is to observe the association of the ABO blood group with Lipid Profile and compare the association in different ABO blood groups. MethodsThis cross-sectional study includes 600 randomly selected subjects from different organizations of district Hyderabad including Universities, Colleges, Hospitals, students, faculty members, and their employees. This study was conducted from February 2021 to January 2022. Informed consent was obtained before the enrolment of volunteers. A structured questionnaire was used to obtain data related to sociodemographic characteristics. A detailed history was taken followed by a thorough clinical examination. All biochemical tests were performed using the blood analyzer and micro lab. Data obtained by structured questionnaire and anthropometric parameters were analyzed by SPSS version 20.0. ResultsIn this study, the Overall B blood group was found more prevalent and similarly found in both males and females. We found Cholesterol higher in the O blood group and Rh-positive subjects.Triglycerides were found higher in B and O and Rh Positive subjects. HDL was found to be higher in B and Rh-positive subjects. LDL was found higher in B and O and Rh Positive subjects. HDL was found to be higher in B and Rh-positive subjects. VLDL was found higher in both B and O AND Rh Positive subjects. ConclusionThe evidence we collected is suggestive of the association of the ABO blood group with Lipid Profile.