It is now emphasized that phytotherapy could treat many cancer types and provide cancer cells with a targeted, efficient treatment. Additionally, it is employed to address the cancer cells' resistance to chemotherapy. The current research aimed to assess the impact of pomelo peel extract on human brain cancer A172 and human pancreas cancer. Citrus maxima L. pomelo peels were extracted using water, while their phytochemical composition was examined using GC-MS and preliminary phytochemical analysis. Human brain cancer A172 and pancreatic cancer are cytotoxic to pomelo peel extract. Capan-2 cell lines were investigated using the MTT test. A multi-parameter cytotoxic assay was carried out to find the extract action on valid cell count, total nuclear intensity, mitochondrial membrane potential, cell membrane permeability, and cytochrome C release utilizing the HCS test. The results demonstrated the extract's richness in several phytochemical constituent categories, such as phenolics, terpenoids, and alkaloids. MTT assay results indicated that IC50 (994.826 µg mL-1) had no effects on the viability of normal mesenchymal cells, whereas A172 cells (IC50 (265.396 µg mL-1) and Capan-2 cells (IC50 (192.247 µg mL-1) exhibited considerable cytotoxicity. Subsequent analysis of the HCS results revealed notable variations in every parameter examined at concentration levels of 200 and 400 µg mL-1. The extract is a strong antioxidant, rich in many therapeutic phytochemical compounds, and highly toxic to A172 and Capan-2 cells, but Capan-2 cells are more sensitive to the extract. Pomelo peel extract didn’t appear toxic to normal mesenchymal cells (HdFn).
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