Α-terpinyl Acetate Research Articles

Assessing the Levisticum officinale Koch. essential oil as a novel preservative for stored chia seeds (Salvia hispanica L.) with emphasis on probable mechanism of action.

The present study was undertaken to explore the inhibitory effect of Levisticum officinale Koch. essential oil (LoffEO) on the growth and aflatoxin B1 secretion by Aspergillus flavus (AF-LHP-SH1, aflatoxigenic strain) causing deterioration of stored chia seeds (Salvia hispanica). The chemical profile analysis of LoffEO by GC-MS analysis revealed the presence of α-terpinyl acetate (26.03 %) as a major component followed by terpineol <1- > (24.03 %) and citronellal (24.03 %). Results on antifungal and antiaflatoxigenic activity indicated that LoffEO at 2.0 and 1.75 μL/mL caused complete inhibition of growth and aflatoxin B1 production, respectively. Antifungal toxicity of LoffEO was strongly correlated with the inhibition of ergosterol content, leakage of cellular ions, and disintegration of membrane permeability. Reduction in cellular methylglyoxal by LoffEO indicated a novel antiaflatoxigenic mechanism of action. The LoffEO showed moderate free radical quenching activity in DPPH assay (IC50 = 26.10 μL/mL) and exhibited remarkable inhibitory efficacy against lipid peroxidation of chia seeds. In addition, LoffEO presented strong in situ antiaflatoxigenic efficacy, and exhibited non-phytotoxic nature, acceptable sensory characteristics, and favorable safety profile (LD50 = 19786.59 μL/kg), which recommends its practical utilization as a novel and safe preservative to improve the shelf life of stored chia seeds from fungal infestation and aflatoxin B1 contamination.

Effective Extraction of Limonene and Hibaene from Hinoki (Chamaecyparis obtusa) Using Ionic Liquid and Deep Eutectic Solvent.

The essential oils of hinoki (Chamaecyparis obtusa) leaves have anti-bacterial, anti-fungal, and relaxation properties that are likely associated with the major components such as sabinene, α-terpinyl acetate, limonene, elemol, myrcene, and hibaene. The present study describes the use of a cellulose-dissolving ionic liquid (IL) [C2mim][(MeO)(H)PO2] and low-toxicity solvents called betaine-based deep eutectic solvents (DESs) for the efficient extraction of hinoki essential oils. As a control method, organic solvent extraction was performed using either hexane, ethyl acetate (EtOAc), or acetone at 30 °C for 1 h. Both the experimental and control methods were conducted under the same conditions, which relied on partial dissolution of the leaves using the IL and DESs before partitioning the hinoki oils into the organic solvent for analysis. Quantitative analysis was performed using gas chromatography–mass spectrometry (GC-MS) in selected ion monitoring (SIM) mode. The results indicated that extraction using the [C2mim][(MeO)(H)PO2]/acetone bilayer system improved the yields of limonene and hibaene, 1.5- and 1.9-fold, respectively, when compared with the control method. In addition, extraction using betaine/l-lactic acid (molar ratio 1:1) gave the greatest yields for both limonene and hibaene, 1.3-fold and 1.5-fold greater, respectively, than when using an organic solvent. These results demonstrate the effective extraction of essential oils from plant leaves under conditions milder than those needed for the conventional method. The less toxic and environmentally begin DESs for the extraction are also applicable to the food and cosmetic industries.

Development of HPTLC method for determination of α-terpinyl acetate, and evaluation of antioxidant properties of essential oils in Elettaria cardamomum

Purpose: To develop a simple and reliable protocol for high performance thin layer chromatography (HPTLC) quantification of α-terpinyl acetate in oils extracted from Elettaria cardamomum, and to study relative antioxidant potential of oils obtained from three varieties of fruits of E. cardamomum.&#x0D; Methods: Essential oil was extracted separately from the fruits of three varieties of E. cardamomum, viz, Valley-green, Palakuzhi, and ICRI-2, using hydro-distillation method. In the development of an HPTLC method, standard α-terpinyl acetate was subjected to chromatography on aluminium-backed silica gel 60 F254 plates using a mobile phase of n-hexane: ethyl acetate (8:2, v/v), and quantified at 665 nm through densitometric analysis. The antioxidant property of essential oil of each cultivar was determined with respect to 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis(3- ethylbenzothiazoline-6-sulphonic acid (ABTS) radical scavenging assays, as well as ferric reducing assay.&#x0D; Results: The developed HPTLC method showed a good resolution for α-terpinyl acetate, with Rf of 0.64 ± 0.01, and also showed good linearity of the calibration plots (r2 = 0.9982). The HPTLC method was validated as per ICH guidelines, and used for the determination of α-terpinyl acetate in the essential oils. The valley green variety of E. cardamomum had the highest content of α-terpinyl acetate (55.36 ± 1.33 %w/w). The inhibitory capacity (IC50 value) for the oil of valley green variety as determined using DPPH and ABTS methods was 378.2 and 19.87 μg/mL, respectively.&#x0D; Conclusion: The proposed HPTLC method is suitable for routine analysis of α-terpinyl acetate in medicinal herbs, and it reveals the role of α-terpinyl acetate in the antioxidant efficacy of cardamom oil.

Antimicrobial Activity of Selected Essential Oils against Selected Pathogenic Bacteria: In Vitro Study.

The worldwide problem of infectious diseases has appeared in recent years, and antimicrobial agents are crucial in reducing disease emergence. Nevertheless, the development and distribution of multidrug-resistant (MDR) strains in pathogenic bacteria, such as Escherichia coli, Staphylococcus aureus, Salmonella Typhi and Citrobacter koseri, has become a major society health hazard. Essential oils could serve as a promising tool as a natural drug in fighting the problem with these bacteria. The current study aimed to investigate the antimicrobial effectiveness of tea tree (Melaleuca alternifolia (Maiden and Betche) Cheel), rosemary (Rosmarinus officinalis L.), eucalyptus (Eucalyptus obliqua L’Hér.), and lavender (Lavandula angustifolia Mill) essential oils. The antimicrobial properties of essential oils were screened against four pathogenic bacteria, E. coli, S. aureus, S. Tyhpi, and C. koseri, and two reference bacterial strains, while for the testing, the agar well diffusion method was used. Gas chromatography (GC) and gas chromatography–mass spectrometric (GC–MSD) analyses were performed on essential oils. The obtained results showed that M. alternifolia essential oil is the richest in terpinen-4-ol, R. officinalis and E. oblique essential oils in 1,8-cineole, and L. angustifolia essential oil in α-terpinyl acetate. In addition, the main bioactive compounds present in the essential oil of tea tree are rich in α-pinene (18.38%), limonene (7.55%) and γ-terpinene (14.01%). The essential oil of rosemary is rich in α-pinene (8.38%) and limonene (11.86%); eucalyptus essential oil has significant concentrations of α-pinene (12.60%), p-cymene (3.24%), limonene (3.87%), and γ-terpinene (7.37%), while the essential oil of lavender is rich in linalool (10.71%), linalool acetate (9.60%), α-terpinyl acetate (10.93%), and carbitol (13.05%) bioactive compounds, respectively. The obtained results from the in vitro study revealed that most of the essential oils exhibited antimicrobial properties. Among the tested essential oils, tea tree was discovered to demonstrate the strongest antimicrobial activity. The recorded MIC of S. Typhi was 6.2 mg/mL, 3.4 mg/mL of C. koseri, 3.1 mg/mL of E. coli, and 2.7 mg/mL of E. coli ATCC 25922, compared to M. alternifolia. Similarly, only S. aureus ATCC 25923 showed antimicrobial activity towards R. officinalis (1.4 mg/mL), E. oblique (2.9 mg/mL), and L. angustifolia (2.1 mg/mL). Based on the obtained results, it is possible to conclude that tea tree essential oil might be used as an ecological antimicrobial in treating infectious diseases caused by the tested pathogens.

Chemical Composition and Antioxidant Activities of Essential oil from Leaf and Stem of Elettaria cardamomum from Eastern India

Elettaria cardamomum (L.) Maton (Zingiberaceae), commonly known as green or true cardamom, is a commercially important species, the seeds of which are extensively used in the pharmaceutical, cosmetic and food industries. In spite of the increasing demand for cardamom essential oil in the international market, the chemical profiling of volatile compounds of different plant parts are not well explored. Though there are a few studies on the chemical characteristics of its leaf oil from other phytogeographical regions, the presence and chemical composition of essential oil from stems have not yet been reported. Therefore, the present study was undertaken to characterize the chemical composition and antioxidant activity of leaf and stem essential oil of E. cardamomum. The essential oil extracted by hydro-distillation from the leaf and stem revealed the presence of 43 and 37 compounds, representing 92.71 % and 92.12 % of total oil respectively by GC/MS analysis. The major constituents of the leaf essential oil were 1,8-cineole (20.66 %), camphene (18.09 %), camphor (10.02 %) and tricyclene (7.36 %); whereas α-terpinyl acetate (19.75 %), 1,8-cineole (10.38 %), caryophyllene oxide (7.13 %) and β-eudesmol (4.85 %) were rich in the stem essential oil. Oxygenated monoterpenes were the major terpenic fraction in the leaf and stem essential oil of E. cardamomum. The free radical scavenging ability assessed by DPPH (2, 2-diphenyl-1-picrylhydrazyl) and ABTS (2, 2-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) assay showed that leaf oil had better activities as compared to stem oil. The present findings demonstrated that E. cardamomum growing wild in Eastern India could be considered as an important bioresource and natural antioxidant.

Essential Oil Profile Diversity in Cardamom Accessions From Southern India

The essential oil of cardamom capsules is a high-value ingredient in foods, beverages, perfumery, and traditional medicines. It is responsible for the characteristic aroma of cardamom. The present study aimed to evaluate essential oil yield and chemical constituents of 22 diverse accessions of cardamom. A total of 20 g of the cured capsules were hydrodistilled in a Clevenger apparatus for 3 h in three replications. The amount of essential oil yield ranged from 4.5 to 9.5%, indicating a substantial variation in this feature among the accessions. The GC/MS analysis results discovered 24 constituents that constituted 98.1–100% of total essential oil. The main fractions were found to be oxygenated monoterpenes (40.7–66.7%), monoterpene hydrocarbons (23.1–58.6%), and sesquiterpenes (0.1–2.0%). Among the monoterpenoids, the predominant constituents were α-terpinyl acetate (29.9–61.3%) followed by 1,8-cineole (15.2–49.4%), α-terpineol (0.83–13.2%), β-linalool (0.44–11.0%), and sabinene (1.9–4.9%). Two sesquiterpene constituents, cardinen and nerolidol and p-cresol (a phenol derivative) were also identified. The compositional data were subjected to euclidean-distance-based similarity analysis, which showed two major clusters. The major constituents of cardamom essential oil (CEO) are 1,8-cineole, α-terpinyl acetate, sabinene, and β-linalool that can be used in food, aroma, and pharmaceutical applications.

Anti-biofilm Potential of Elletaria cardamomum Essential Oil Against Escherichia coli O157:H7 and Salmonella Typhimurium JSG 1748.

Foodborne pathogens, microbial recurrent infections, and antibiotic resistance have driven researchers to explore natural compounds as safe alternative antimicrobials. In this study, the chemical profile, antimicrobial, and mutagenic activities of the Elletaria cardamomum essential oil were investigated. GC-MS analysis identified the major bioactive components as α-terpinyl acetate, 1,8-cineole, linalool acetate, and sabinene, at concentrations of 34.95, 25.30, 8.13, and 5.48% respectively, of the essential oil’s content. Regarding antimicrobial activity, the minimum inhibitory concentration of green cardamom essential oil was 1% against Escherichia coli O157:H7 and Pseudomonas aeruginosa ATCC 14213. Green cardamom essential oil, when used at concentrations of 0.015, 0.031, 0.062, and 0.125% (v/v) prevented biofilm formation of Escherichia coli O157:H7 by 64.29, 65.98, 70.41, and 85.59%, respectively. Furthermore, these concentrations inhibited 6.13, 45.50, 49.45, and 100%, respectively, of the Salmonella Typhimurium JSG 1748 biofilm. A mutagenicity assay confirmed that green cardamom essential oil has no demonstrable mutagenic activity against the tested strains. The study’s findings suggest that green cardamom derived bioactive compounds are safe organic antimicrobials, effective in controlling biofilm formation by Gram-negative pathogens. Moreover, such compounds could possibly be used in the food industry (e.g., bakery, dairy, meat, and other food products) as a safe alternative to chemical preservatives (antimicrobials) to enhance shelf life by improving the antimicrobial status while at the same time imparting a pleasant and appealing aroma for consumers.

Essential Oil Composition, Cytotoxicity against Hepatocellular Carcinoma, and Macro and Micro-Morphological Fingerprint of Laurus nobilis Cultivated in Egypt

GC/MS analysis of hydro-distillated essential oil (1.9% v/w) from fresh leaves of Laurus nobilis cultivated in Egypt revealed the presence of 81 components from which 59 compounds were identified, the identified compounds represent 96.3 % of the oil composition. The major constituents were 1,8-cineole (27.41%), linalool (19.37%), α-terpinyl acetate (14.65%), methyl eugenol (7.73%), α-terpineol, (2.84%), terpinen-4-ol (2.09%), elemicin (2.08%) and sabinene (1.80%). Qualitative and quantitative identification of the essential oil constituents was based on comparison of retention indices (RIs) of standard n-alkanes (C8-C24) and mass spectra of the oil components with those reported in the literature. The essential oil exhibited potent cytotoxic activity against Hep-G2 (IC50= 1.83μg/mL). The cytotoxic activity study was enriched using molecular docking analysis of the oil components against caspase3 (apoptotic enzyme). The docking reflected the ability of 32 compounds to interact with caspase3 at different sites and initiating its activity. Moreover, macro- and micromorphological studies of the plant’s leaves, petioles and stem in both entire and isolated elements forms were carried out. They reflected the presence of characteristic oil cells and fibers in the leaves, petioles and stems in addition to mucilage cells in the leaves and petioles, sclereids in the leaves and old stems and small trichomes on the young stem surface.

Comparative Study of Chemical Composition and Antioxidant Activity of Essential Oils and Crude Extracts of Four Characteristic Zingiberaceae Herbs.

The ginger family (Zingiberaceae) includes plants that are known worldwide to have a distinctive smell and taste, which are often used as spices in the kitchen, but also in various industries (pharmaceutical, medical, and cosmetic) due to their proven biological activity. The aim of this study was to investigate and compare the chemical composition and antioxidant activity (AA) of essential oils (EOs) of four characteristic ginger species: Elettaria cardamomum L. Maton (cardamom), Curcuma Longa L. (turmeric), Zingiber Officinale Roscoe (ginger), and Alpinia Officinarum Hance (galangal). Furthermore, the total phenolic content (TPC) and AA of crude extracts obtained after using ultrasound-assisted extraction (UAE) and different extraction solvents (80% ethanol, 80% methanol and water) were evaluated. A total of 87 different chemical components were determined by GC-MS/MS in the EOs obtained after hydrodistillation, 14 of which were identified in varying amounts in all EOs. The major compounds found in cardamom, turmeric, ginger, and galangal were α-terpinyl acetate (40.70%), β-turmerone (25.77%), α-zingiberene (22.69%) and 1,8-cineol (42.71%), respectively. In general, 80% ethanol was found to be the most effective extracting solvent for the bioactivities of the investigated species from the Zingiberaceae family. Among the crude extracts, ethanolic extract of galangal showed the highest TPC value (63.01 ± 1.06 mg GA g−1 DW), while the lowest TPC content was found in cardamom water extract (1.04 ± 0.29 mg GA g−1 DW). The AA evaluated by two different assays (ferric-reducing antioxidant power-FRAP and the scavenging activity of the cationic ABTS radical) proved that galangal rhizome is the plant with the highest antioxidant potential. In addition, no statistical difference was found between the AA of turmeric and ginger extracts, while cardamom rhizome was again inferior. In contrast to the crude extracts, the EOs resulted in significantly lower ABTS and FRAP values, with turmeric EO showing the highest AA.

α-Terpinyl Acetate: Occurrence in Essential Oils Bearing Thymus pulegioides, Phytotoxicity, and Antimicrobial Effects.

The aim of this study was to evaluate occurrence of T. pulegioides α-terpinyl acetate chemotype, as source of natural origin α-terpinyl acetate, to determine its phytotoxic and antimicrobial features. Were investigated 131 T. pulegioides habitats. Essential oils were isolated by hydrodistillation and analyzed by GC-FID and GC-MS. Phytotoxic effect of essential oil of this chemotype on monocotyledons and dicotyledons through water and air was carried out in laboratory conditions; the broth microdilution method was used to screen essential oil effect against human pathogenic microorganisms. Results showed that α-terpinyl acetate was very rare compound in essential oil of T. pulegioides: it was found only in 35% of investigated T. pulegioides habitats. α-Terpinyl acetate (in essential oil and pure) demonstrated different behavior on investigated plants. Phytotoxic effect of α-terpinyl acetate was stronger on investigated monocotyledons than on dicotyledons. α-Terpinyl acetate essential oil inhibited seeds germination and radicles growth for high economic productivity forage grass monocotyledon Poa pratensis, but stimulated seed germination for high economic productive forage legume dicotyledon Trifolium pretense. α-Terpinyl acetate essential oil showed high antimicrobial effect against fungi and dermatophytes but lower effect against bacteria and Candida yeasts. Therefore, T. pulegioides α-terpinyl acetate chemotype could be a potential compound for developing preventive measures or/and drugs for mycosis.

PHYTOCHEMICAL CONSTITUENTS, ANTIOXIDANT, ANTIBACTERIAL AND ENZYME INHIBITION ACTIVITIES OF ESSENTIAL OIL FROM THE AERIAL PART OF Achillea tomentosa L. GROWN IN JORDAN

This investigation aimed to evaluate the phytochemical constiuents, antioxidant, antibacterial and α-glucosidase inhibitory activities of A. tomentosa essential oil. The essential oil extracted using steam distillation method possessed antibacterial activity for all tested bacteria. Comparing to cefaclor the percentage of relative inhibition zone diameter (RIZD) was 100.30 ± 4.73 % for Staphylococcus aureus and ranged between 79.67 ± 3.79 % and 84.33 ± 4.16 % for other bacteria. The essential oil showed considerable antioxidant activity using 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging activity, ferric reducing antioxidant power (FRAP) assay and metal chelating methods, the EC50 of the essential oil were 72.06 ± 3.99, 54.69 ± 4.69 and 50.39 ± 2.86 µg/ml respectively. Comparing to EC50 7.34 ± 2.43, 10.87 ± 3.34 and 14.50 ± 3.46 µg/ml for positive controls (Trolox, EDTA and Quercetin respectively). The essential oil also possessed concentration dependent α-glucosidase inhibitory activity with EC50 149.20 ± 7.63 µg/ml comparing to acarabose EC50 14.50 ± 3.46 µg/ml. Forty compounds were identified depending in retention index (RI) and mass spectra (MS) that form 96.31 %of the essential oil composition. The result showed that the ratio of the identified compounds range between 0.11 % and 23.72 %, 3-Carene (23.72 %), Limonene (12.19 %) and α-Terpinyl acetate (10.22%) were found to be the major components of the essential oil. m-Cymene (7.11 %) and Di-n-octyl phthalate (4.50 %) present in moderate quantities. The phytochmical analyzes showed that the identified compound quantitatively and qualitatively differ from the previous study that investigate the composition of A.tomentosa L. essential oil.

In vitro antioxidant and anti-inflammatory activities of green cardamom essential oil and in silico molecular docking of its major bioactives

Essential oil of Elettaria cardamomum (Green cardamom) is effective in the treatment of various disease conditions. Globally, Guatemala is the leading producer of green cardamom fruits. Green-cardamom fruits from Guatemala were purchased from a Saudi Arabian market. The Guatemala cardamom essential oil (GCEO) was extracted using hydro-distillation method, and analyzed using gaschromatography/ mass-spectrometry (GC-MS). The antioxidant and anti-inflammatory activities were studied using 2,2-diphenyl-1-picrylhydrazyl-hydrate, ferric-chloride, bovine serum-albumin and proteinase inhibitory assays. GC-MS results confirmed the presence of α- terpinyl acetate and 1,8-cineole as major components. The antioxidant and anti-inflammatory activities of GCEO were found to be significant compared with those of standard ascorbic acid and ibuprofen, respectively. The molecular-docking study revealed the considerable binding potential of α-terpinyl acetate and 1,8-cineole with human-peroxiredoxin-5, tyrosine-kinases and Human-5-LOX receptors. The in-silico activity prediction analysis confirmed the antioxidant and anti-inflammatory potential, whereas the pharmacokinetics confirmed the safety of most of the GCEO compounds.

Development of QCM sensor to detect α-terpinyl acetate in cardamom

α-terpinyl acetate (aTA) is an organic volatile compound present in cardamom and it majorly makes this spice gracefully flavoured. Quartz crystal microbalance (QCM) sensor with tailor-made sensitivity provides a wide range of linear responses and is generally used for aroma sensing based on the deviation of the resonant frequency. In this present work, a QCM gas sensor (aTA-MIP-QCM) has been developed by coating the quartz crystal with a molecularly imprinted polymer (MIP) containing polymethacrylic acid (PMAA) to detect α-terpinyl acetate in small cardamom. Extensive experimentation has been carried out for the study of sensitivity, selectivity, repeatability, reproducibility, and stability of the decorated sensor and the results demonstrate that the sensor is exclusively sensitive and selective to aTA. The sensor was exposed to the aroma of cardamom samples and a significant correlation was obtained of the sensor response with the gas chromatography-mass spectrometry (GC–MS) estimations.

Comparative study on the chemical composition of laurel (Laurus nobilis L.) leaves from Greece and Georgia and the antibacterial activity of their essential oil

Laurel (Laurus nobilis L.) is a plant species from Lauraceae family, and is native to the Mediterranean region. The goal of this study was to compare chemical composition of laurel leaves and antibacterial activity of its essential oil (EO) from wild-grown trees in Greece and Georgia. The laurel leaves from the two native habitats had dissimilar concentrations of phenolic acids. Of the conjugated flavonols and flavons, kaempferol (1981.3 μg/g) and apigenin (1433.6 μg/g) were the major representatives in the leaves from Greece, while luteolin (839.1 μg/g) and kaempferol (688.1 μg/g) were the major ones in the leaves from Georgia, respectively. The EO content was 1.42% and 4.54% in the leaves from Greece and Georgia, respectively. The main EO constituents of the Greek laurel plants were 1,8-cineole (30.8%), α-terpinyl acetate (14.9%), α-terpineol (8.0%), sabinene (7.9%), and terpinen-4-ol (6.0%). The main EO constituents of the Georgian laurel plants were 1,8-cineole (29.2%), α-terpinyl acetate (22.6%), sabinene (12.2%), and methyleugenol (8.1%). The EO antimicrobial activities against 20 microorganisms were determined. Among the Gram-positive bacteria, the Enterococcus faecalis strain was the most sensitive, followed by Staphylococcus aureus ATCC 6538. Among the Candida species, C. albicans ATCC 10231 was the most sensitive to the laurel leaf EOs.

A Study of Essential Oil Constituents from Capsules, Physiological and Quality Parameters of Small Cardamom Ecotypes [Elettaria cardamomum (L.) Maton] Growing in the Western Ghats, India

An experiment was conducted at the Indian Institute of Spices Research, Regional Station, Madikeri, Karnataka, to study physiology, yield and quality responses of six small cardamoms [Elettaria car- damomum (L.) Maton] genotypes (Appangala-1, PV-1, ICRI-2, FGB-34, PV-2 and Green Gold) selected from three ecotypes (Malabar, Mysore and Vazhukka) during 2018-2019. The experiment was designed in Randomized Complete Block Design (RCBD) with three replications. Results showed Green Gold registered a more dry capsule yield (673.8 kg ha-1) followed by Appangala-1 (576.4 kg ha-1). Physiological attributes like photosynthetic rate, stomatal conductance, epicuticular wax and total chlorophyll content were higher for Green Gold closely followed by Appangala-1. Canopy temperature was lower considerably in both genotypes. Both Appangala-1 and Green Gold reported a higher concentration of 1,8-cineole accordingly 43.9 % and 40.8 %. Therefore, these types impart higher camphorous odor compared to other genotypes. Other genotypes PV-1 and ICRI-2 succinctly had a higher quantity of α-terpinyl acetate (41.6 % and 40.3 % respectively) that conveys their sweet pleasant aroma. The volatile oil of PV-2 and FGB-34 possessed a higher proportion of α-terpinyl acetate (37.9 % and 37.9 % in order) and linalool (11.1 % and 7.1 %, correspondingly) which render the sweet aroma and acceptable flavor quality to these genotypes. Other compounds like sabinene, myrcene, terpinen-4-ol, α-terpineol and geraniol showed little variation among the genotypes studied. The results of the study highlight Green Gold as a physiologically superior and high yielding genotype and PV-2 as a better quality genotype for its supremacy of pleasant sweet aroma.

Black cardamom essential oil prevents Escherichia coli O157:H7 and Salmonella Typhimurium JSG 1748 biofilm formation through inhibition of quorum sensing.

This study aimed to investigate the chemical composition, using GC-MS, and anti-biofilm potential of black cardamom essential oil (BCEO) against biofilms of Escherichia coli O157:H7 and Salmonella Typhimurium JSG 1748 through inhibition of bacterial quorum sensing. GC-MS quantification demonstrated that BCEO contains 1,8-cineole (44.24%), α-terpinyl acetate (12.25%), nerolidol (6.03%), and sabinene (5.96%) as the major bioactive compounds. Antioxidant assays for BCEO revealed the total phenolic and flavonoid mean values were 1325.03 ± 7.69mg GAE 100/g and 168.25 ± 5.26mg CE/g, respectively. In regards to antimicrobial potential, Candida albicans was the most sensitive species compared to Streptococcus mutans, Staphylococcus aureus, Listeria monocytogenes, Bacillus cereus, and Salmonella Typhimurium with the following zones of inhibition; 14.4 ± 0.52, 13.2 ± 0.42, 11.2 ± 0.28, 11.0 ± 0.52, 8.2 ± 0.24 and 6.6 ± 0.18mm in diameter, respectively. Biofilm inhibition by BCEO was concentration-dependent, when various concentrations of 0.03, 0.06, 0.12, 0.25 and 0.5% were applied, 33.67, 34.14, 38.66, 46.65 and 50.17% of Salmonella Typhimurium biofilm was inhibited, while 47.31, 54.15, 76.57, 83.36 and 84.63% of Escherichia coli biofilm formation was prevented. Chromobacterium violaceum ATCC 12,472 and its product violacein, was used as a microbial indicator for enhancement or inhibition of quorum sensing. Our data showed that 0.5% of BCEO inhibited violacein production without influencing the growth of Chromobacterium violaceum, while 1% of BCEO, caused 100% inhibtion of violacein production together with 30% inhibition of growth. This study shows that BCEO possesses promising antioxidant and antimicrobial potential, and found anti-biofilm activities linked to the quenching of the quorum sensing system of E. coli and S. Typhimurium.

Thermochemical Properties of Selected Terpenes

This work is a continuation of our project of physico-chemical characterization of terpenic compounds participating as a source of secondary organic aerosols. In this work, S-(–)-limonene, terpinolene, (1R)-(–)-myrtenal, and α-terpinyl acetate were studied. Their densities, sound velocities, and isobaric heat capacities were measured in the temperature range (290–343) K, vapor pressures were measured in temperature range where stable boiling was achieved. The previous work was supplemented by measurement of isobaric heat capacities of α-pinene and β-pinene. Experimental values of vapor pressures were compared with prediction by COSMO-RS. Modern nonstatistical algorithms based on mathematical gnostics were used for data analysis.

Gender Dependent Differences in Composition, Antioxidant and Antimicrobial Activities of Wild and Cultivated Laurus nobilis L. Leaf and Flower Essential Oils from Aegean Region of Turkey

Laurus nobilis L. is a dioecious species that have separate female and male plants. The Aegean region of Turkey is a common place for laurel where it is cultivated for industrial purposes and wildly grown. In this study, we aimed to determine the gender-specific properties of laurel leaf and flower essential oils by means of chemical compositions and biological activities. In both genders -whether it is cultivated or notlaurel leaves presented the same yield of essential oils having almost the same compositions. According to the results, we found that the differences in yields and compositions of flower essential oils were only affiliated with gender. All essential oils performed mild antioxidant activities and significant antimicrobial effects yet essential oils of leaves were much more superior in every aspect. The alterations in the chemical composition of flower essential oils from different genders did not affect the overall biological activities so we presumed that trans-ocimene and α-terpinyl acetate were not effectual in antioxidant and antimicrobial capabilities.

Chemical Diversity of Volatile Compounds of Mints from Southern Part of Pannonian Plain and Balkan Peninsula - New Data.

Mints are the most popular economic and traditional herbs. The aim of this article was chemical characterization of volatile compounds from wild populations of Mentha aquatica, M. arvensis, M. longifolia, M. microphylla, M. pulegium, M. spicata, M.×dumetorum, M.×gentillis and M.×verticillata, as well as cultivated samples of M. spicata, M.×piperita 'Alba' and M.×piperita 'Crispa'. Analyses were performed directly from dried aerial parts (herb) of collected samples by headspace gas chromatography-mass spectrometry. In total 54 compounds were detected, representing from 89.99 % to 99.66 % of volatile fractions of all investigated samples. The recorded volatiles were primarily monoterpene hydrocarbons and oxygenated monoterpenes, while oxygenated aromatic monoterpenes, sesquiterpene hydrocarbons and aliphatic compounds were present in lower concentrations in analyzed samples. The major components were linalool, limonene, 1,8-cineol, α-terpinyl acetate, pulegone, β-pinene and menthol. The cluster analysis revealed five main groups or chemotypes according to qualitative and quantitative content of volatiles, as well as similarities among samples. These results contribute to the knowledge on the mints chemistry in Pannonian Plain and Balkan Peninsula.

Seasonal variations of essential oils from five accessions of Mentha longifolia (L.) L. with selected chemical profiles

ABSTRACT Essential oil (EO) yield and composition of five accessions of Mentha longifolia (L.) L. (leaves) were determined throughout their vegetative cycle by GC-MS and GC-FID analysis. These accessions were selected based on TLC profiles of dichloromethane extracts from wild individuals. The profile rich in α-terpinyl acetate and 8-acetoxy carvotanacetone has not been previously reported. The maximum value of EO yield was obtained in advanced flowering stage. The major composition changes were noted at the beginning of the vegetative cycle, whereas more stability was found during the flowering stage. Thus, significant changes in the major compounds were found in some accessions: piperitone and piperitenone oxides, α-terpineol acetate and 8-acetoxy carvotanacetone, which showed a considerable increase (6.0% to 20.1%) during the vegetative growth, the same way that pulegone and piperitenone oxide in other accessions. As biological activities depend on EO composition, the knowledge of its seasonal changes should be emphasized.