10th Day Research Articles

A rare case of steroid-resistant nephrotic syndrome complicated by Wernicke's encephalopathy.

Wernicke's encephalopathy (WE) is a severe neurological condition caused by the deficiency of thiamine, which is a vitamin B1 molecule. Herein, we present the case of a 3-year-old girl with steroid-resistant nephrotic syndrome (SRNS) who did not achieve remission despite steroid pulse therapy (MPT) and rituximab. She had frequent vomiting and decreased oral intake on the 61st day. High-calorie total parenteral nutrition was initiated on the 86th day, and levothyroxine was administered for suspected hypothyroidism. On the 88th day, she experienced altered consciousness with ocular deviation and abnormal electroencephalography findings, raising suspicion of WE. Following thiamine supplementation, recurrence of symptoms and neurological sequelae were not observed. Following 10 courses of MPT, rituximab, and mycophenolate mofetil, the patient achieved incomplete remission from SRNS. Monitoring levels of water-soluble vitamins in prolonged cases of nephrotic syndrome, such as SRNS, is critical to prevent WE and irreversible neurological damage.

Performance of Camellia oleifera Seedlings Under Alkali Stress Improved by Spraying with Types of Exogenous Biostimulants

Exogenous biostimulants (EB) are crucial for reducing abiotic stress in plants. It is currently unclear how EB such as melatonin (MT), betaine (BA), and salicylic acid (SA) regulate the stress in Camellia oleifera seedlings under alkali stress (XP). This study demonstrates the moderating effect of SA (0.5, 1, and 2 mmol/L), BA (0.2, 0.4, and 0.8 g/L), and MT (200, 400, and 800 μmol/L) on the relative chlorophyll content, photosynthetic parameters, chlorophyll fluorescence parameters, osmoregulatory substances, and antioxidant enzymes in C. oleifera seedlings under XP. The results showed that spraying different types and different concentrations of EB under alkali stress had a certain alleviating effect on the phenotype of C. oleifera seedlings. Whether 7 or 15 days after the application of EB, the relative chlorophyll content (SPAD) and the degree of yellowish-green in the control group were different from those in the other 10 treatment groups, but the difference in brightness was not significant. As far as the malondialdehyde (MDA) content is concerned, the SA2, BA3, MT2, and MT3 treatment groups can significantly reduce the MDA content on the 7th day of EB application. The electrolytic leakage (EL) is also significantly reduced by MT2 and MT3. It was found that treatment groups SA3 and MT2 could improve the photosynthetic parameters of C. oleifera seedlings to different degrees on the 7th day of EB application. On the 15th day of EB application, treatment groups SA1, SA3, BA1, and BA2 all increased the photosynthetic rate of C. oleifera compared to the XP treatment group, but other treatments did not increase. At the same time, the results showed that the fluorescence parameters of the seedlings showed different degrees of improvement under different EB spraying conditions. Under alkali stress, soluble proteins (SP) and soluble sugars (SS) increased in the XP group, but it was found that the SA3, BA3, and MT2 treatment groups could reduce the content of osmoregulatory substances both on the 7th and 15th days of EB application. In terms of proline (Pro) content, BA1, BA2, and MT2 treatment groups could reduce Pro content on the 7th and 15th days of EB spraying, respectively. As for the antioxidant enzymes, the SA2, BA3, MT2, and MT3 treatment groups could basically increase the activity of antioxidant enzymes and further reduce oxidative damage on the 7th day of application of EB. According to the comprehensive results of the membership function, whether on the 7th or 15th day of EB spraying, the MT2 treatment group has the best overall mitigation effect of the three EB applications, ranking in the top three. This study will help to improve the scientific understanding of C. oleifera’s alkali resistance and interaction with EB while filling the knowledge gap on the physiological response to oleofylline stress.

Effect of Exogenous γ-Aminobutyric Acid (GABA) on the Growth, Photosynthetic Pigment, Antioxidant and GABA Metabolism of Festuca arundinacea (Tall Fescues) Under Cadmium Stress

γ-Aminobutyric acid (GABA), an endogenous amino acid widely found in living organisms, has important functions in plants such as regulating growth and development, maintaining carbon and nitrogen nutrient balance, and coping with adversity. In this study, we investigated the effects of exogenous 0.5 mmol/L GABA on the growth, antioxidant metabolism, and GABA shunt metabolism of tall fescue under 20 μmol/L Cd stress, using tall fescue (Festuca arundinacea) ‘Ruby II’ under hydroponics conditions. The results showed that (1) applying GABA for 3, 7, 11, and 15 d under Cd stress inhibited Cd transport from roots to leaves and promoted plant height, alleviating the effects of Cd stress on plant growth. (2) Exogenous 0.5 mmol/L GABA had an interesting regulatory effect on the activation of the antioxidant enzyme system induced by stress at different stages, which was accompanied by a decrease in malondialdehyde (MDA) contents and alleviated the degree of cell membrane lipid peroxidation under cadmium stress. Specifically, peroxidase (POD) enzyme activity reactions initially responded on the 3rd and 7th days of stress, and the changes in catalase (CAT) enzyme activities concentrated on the 11th and 15th days of the later stage. Ascorbate peroxidase (APX) enzyme was active throughout the whole stress period in the roots. Multiple factorial analyses further proved that the antioxidant pathway strongly influenced the survival and growth of tall fescue under stress in the presence of GABA. (3) Application of exogenous GABA activated the branching pathway for GABA synthesis from Glu decarboxylation (GABA shunt) with a higher contribution in the leaves, which induced changes in glutamate content, and plants maintained a higher endogenous GABA content and signal to regulate the plant antioxidant system and reduce cell membrane damage, thus improving the tolerance of plants to Cd stress.

P-40 A RARE PRESENTATION OF PROLONGED HYPOGLYCEMIA: CONGENITAL LEPTIN DEFICIENCY AND INSULIN INTOXICATION

Abstract Introduction Congenital leptin deficiency (CLD) is caused by mutations in the LEP gene with an incidence of 1 in 4.4 million, presenting with symptoms such as hyperphagia, obesity. Insulin intoxication is also a rare condition that usually occurs as a result of a suicide attempt or, a medication error. There is not an algorithm for the management of insulin intoxication. Here, we report the only case in the literature presenting with congenital leptin deficiency and insulin intoxication. Clinical Case A 33-year-old woman was admitted to the hospital with dizziness, nausea, and unconsciousness. She had applied nearly 300 IU insulin aspart of committing suicide. She had congenital leptin deficiency, hypothyroidism, and prediabetes. She had leptin replacement during 2020-2021 and didn’t have a replacement since then. Her body mass index was 62.2. Her blood pressure was 95/60 mm/Hg, Sp02 was 88 %, and heart rate was 120 per minute. Her blood glucose was 29 mg/dl, and there was respiratory acidosis. Liver and renal function tests, electrolytes, coagulation parameters, and thyroid hormones were normal. Chest x-ray and brain tomography were normal. Despite subsequent infusion of 20% dextrose solution via central catheter, serum glucose could only be kept at 70-80 mg/dl. In the following hours patient’s consciousness, pH level, and oxygen saturation returned to normal. We had to follow the patient with a high concentration of glucose (150-200cc/h %10 dextrose) for ten days because of reappearing hypoglycemia. On the 10th day of follow-up, we stopped IV glucose for a short time and measured the fasting hormones shown in Table 1. at 7.00 a.m. As there was hypocortisolemia, we applied 5mg prednisolone twice daily in the following days. Twenty-four hours after cortisol administration, we could easily stop the IV glucose. We followed the patient with 5mg prednisolone twice daily and oral carbohydrate intake for three days and didn’t observe hypoglycemia. Afterwards, we stopped prednisolone and the patient was normoglycemic for the following days. We again measured the fasting hormones shown in Table 2. At 7.00 a.m. three days after the last dose of prednisolone. The patient was not hypoglycemic or hypocortisolemic since then; vital parameters were all normal and we discharged the patient. Conclusion Prolonged hypoglycemia may have occurred in different mechanisms. Our patient had a thick subcutaneous fat tissue because of CLD; therefore, absorption of insulin may have increased and half life may be prolonged. Also, high dose exogenous insulin may have triggered the production of insulin antibodies causing prolonged duration of insulin action and hypoglycemia. Moreover, deep hypoglycemia may have affected the pituitary-adrenal cortisol axis causing relative adrenal insufficiency. The steroid decreased the insulin antibodies and also corrected the relative cortisol deficiency. More cases and data are needed to shed light on this subject.Table 1: Table 2:

Lactobacillus rhamnosus Sepsis in a Preterm Infant Following Probiotic Administration: Challenges in Diagnosis

Probiotic administration has become common practice in neonatal intensive care units (NICUs) to prevent necrotizing enterocolitis (NEC) and promote gut health in preterm infants. While probiotics are generally considered safe, rare cases of probiotic-related sepsis have been reported. We present a case of Lactobacillus rhamnosus sepsis in a preterm infant, highlighting the challenges involved in its diagnosis. The infant developed symptoms of sepsis on the 13th day of probiotic treatment. Laboratory analyses, including MALDI-TOF, BioFire BCID2 panel, and whole-genome sequencing (WGS), helped confirm the diagnosis and the presence of Lactobacillus rhamnosus. In this case, accurately identifying the Lactobacillus rhamnosus strain proved challenging, as initial analyses using the Vitek 2 system yielded incorrect identifications. This highlights the limitations of automated systems in distinguishing closely related species, reinforcing the need for advanced molecular techniques to achieve precise strain identification and confirm a probiotic-related infection. Given these diagnostic complexities, it is crucial for clinicians to maintain a high index of suspicion for probiotic-related infections in cases of unexplained sepsis, as this awareness can prompt further diagnostic investigations to ensure accurate pathogen identification. The infant responded to ampicillin therapy, showing clinical improvement within 10 days and was discharged in good health at 67 days of life. This case underscores the importance of advanced molecular diagnostic methods to confirm probiotic-related infections and highlights the need for caution in administering probiotics to vulnerable populations, such as preterm infants. Clinicians must maintain a high index of suspicion for probiotic-associated sepsis in unexplained cases of infection and tailor antibiotic therapy based on susceptibility profiles. These findings emphasize the need for rigorous monitoring, appropriate probiotic strain selection, and optimized safety protocols in NICUs to mitigate potential risks.

Effectiveness of the use of a plant immunostimulator for feline bordetelliosis

In veterinary practice, veterinary specialists solve the important problem of the occurrence and development of immunodeficiency during respiratory infectious pathology in cats with the help of biologically active additives, probiotics, enzymes, immunostimulators and vitamins. Interferons are one of the most popular in use from the list of endogenous immunomodulators. But often the disadvantage of purely immunomodulatory drugs is their difficult transfer and the presence of a negative effect on the body of domestic animals. Therefore, an alternative to interferons in order to increase the immune resistance of the body and improve the well-being of cats is the search, development and use of preparations based on plant raw materials. The article presents the results of the study of the effectiveness of the herbal immunomodulator in comparison with the interferon drug "Anfluron", which was used in two complex treatment schemes for cats suffering from Bodetheliosis infection. To increase the immune resistance of cats for bordeteliosis, an aqueous solution of a medicinal composition based on the Rhodiola rosea root, Oplopanax root, Rozae fruits, Urtica dioica, Crataegus fruits, Hypericum perforatum fruits was used in an effective ratio of 1:1:1:0.8:0.8:0.5. As a result of the use of the specified herbal preparation, a positive trend in the reduction of the manifestation of clinical signs in sick cats was established from the 6th day of use with a direct correlation of the decrease in the manifestation of clinical signs in sick animals treated with a classic interferon drug. As a result of the application of complex therapeutic regimens using various immunomodulatory drugs, a significant alignment of hematological indicators to the physiological norm was noted on the 10th and 14th day of treatment in animals of both study groups. The results obtained in the comparative aspect showed that the proposed herbal preparation has an immunostimulating effect, improves the general condition and can be used in the complex treatment of cats with bordetelliosis.

The relationship between the well-being and fatness of sows with a fixed content on the farrowing site in the conditions of industrial technology

The aim of the study was to assess the well-being of sows at a farrowing site with a fixed content in the conditions of industrial pork production technology. The object of the study was suckling sows contained in BDSwing machines on lattice floors. The Welfare Quality® protocol was used for the assessment, modified in accordance with local production features. The well-being of the sows was assessed on the first day after farrowing and again on the 15th day of the suckling period. When processing the data, the total welfare score of the sows was calculated. The fatness of the sows was determined by the thickness of the fat above the last rib on the 30th day of pregnancy and at weaning at 28 days. The statistical analysis was performed using the statistical programming environment R. The reliability of differences between groups of animals in fatness on the 30th day of pregnancy and at weaning was assessed by the Kraskel-Wallis method. Pairwise comparisons were carried out with the Hill correction. In the group of well–off sows, the total score was 0.62 points, while for the conditionally well-off, this parameter was already 2.51 points and the disadvantaged – 4.9 points. The re-evaluation was carried out on the 15th day after farrowing by the same researcher. With minor changes in the total assessment of animals (0.59, 2.49 and 5.12 points, respectively), there is an increase in the total assessment in the group of disadvantaged sows, as well as the movement of animals from one group to another in the direction of aggravation of the condition. Significant differences in fatness between well-off and conditionally well-off and dysfunctional sows on the 30th day of pregnancy were determined. At the same time, although healthy sows differed in the smallest fat thickness, during pregnancy and suckling period, in this group the least loss of fatness to weaning was noted (-0.3 mm).

Effect of Arum rupicola Boiss rupicola Extracts on Visceral Larva Migrans in Mice

PurposeIn the present study, the effects of leaf and rhizome extracts of Arum rupicola Boiss rupicola were searched on the infective stage Toxocara canis larvae (L3) in the experimentally infected mice.MethodsFour-six week-old male BALB/c mice were divided into eight groups (G1-8, each group consisted of 7 mice), and they were infected orally with 500 T. canis eggs with L3. After 24 h, the following treatment protocol was applied to the groups for five days: G-1: Albendazole (ABZ) (100 mg/kg), G-2: Leaf Extract (10 mg/mL) + ABZ (100 mg/kg), G-3: Leaf Extract (40 mg/mL) + ABZ (100 mg/kg), G-4: Rhizome Extract + ABZ (100 mg/kg), G-5: Leaf Extract (10 mg/mL), G-6: Leaf Extract (40 mg/mL), G-7: Rhizome extract, G-8: Negative control. The necropsy was performed on the 45th day of experimental infection.ResultsThe rhizome extract significantly reduced the effectiveness of ABZ compared to leaf extracts. The number of larvae in rhizome extract-treated mice (G7) was similar to ABZ-treated mice (G1) (P < 0.05). The larval number in mice administered leaf extract (10 mg/mL and 40 mg/mL) was slightly decreased. But no significant difference was detected in the larval number depending on the dose of leaf extracts (P > 0.05).ConclusionThe administration of the leaf and rhizome extracts did not contribute to the effectiveness of ABZ. Also the effect of ABZ on the larvae number obtained from the tissues was reduced by the rhizome extract. The larvae number in the group in which rhizome extract was given alone was almost close to the group in which ABZ was administered. The results provide insight for further research on the antiparasitic properties of A. rupicola Boiss rupicola rhizome extract.

The cell colony development is connected with the accumulation of embryogenesis-related proteins and dynamic distribution of cell wall components in in vitro cultures of Fagopyrum tataricum and Fagopyrum esculentum

BackgroundDue to the totipotency of plant cells, which allows them to reprogram from a differentiated to a dedifferentiated state, plants exhibit a remarkable regenerative capacity, including under in vitro culture conditions. When exposed to plant hormones, primarily auxins and cytokinins, explant cells cultured in vitro can undergo differentiation through callus formation. Protoplast culture serves as a valuable research model for studying these processes in detail. This knowledge is particularly relevant for improving common and Tartary buckwheat species. To gain deeper insights into the stages of cell development from protoplasts—such as cell division, cell colony formation, and microcalli development—we focused on analyzing proteomes, cell wall composition, and changes in the expression profiles of selected genes in Fagopyrum protoplast cultures.ResultsThe results demonstrate a significant accumulation of somatic embryogenesis-related proteins like late embryogenesis abundant proteins (embryogenic protein-DC-8-like, seed biotin-containing protein) and endochitinases during the developmental path of protoplast-derived cultures. Additionally, we noted an extensive increase in seed storage proteins like vicilin, oleosins, and seed biotin-containing proteins during the culture. Investigation of somatic embryogenesis-associated transcription factors revealed massive up-regulation of LEAFY COTYLEDON1 for the 50th day of F. tataricum protoplast-derived cultures. However, for BABY BOOM, the transcription factor was noted to be down-regulated during the development of cell colonies. Furthermore, we demonstrated the variable distribution of cell wall components like pectin side chains, arabinogalactan proteins (AGPs) and extensins (EXTs), indicating the reorganisation of cell wall composition during the culture period.ConclusionsThis study revealed changes correlating with regaining embryogenic competence during the development of Fagopyrum protoplast-derived cell colonies. Our findings revealed variable expression levels of genes and proteins associated with somatic embryogenesis. This analysis identified an increase in seed storage proteins that play a significant role in the somatic somatic embryogenesis pathway of regeneration. Furthermore, the relationship between transcription factors and these processes seems to be connected with regaining somatic cells’ totipotency and promoting embryogenic competence of protoplast-derived cell colonies. Additionally, we observed dynamic changes in cell wall composition during the development of the protoplast-derived cultures.Clinical trial numberNot applicable.

Alleviation of Acute Heat Stress in Broiler Chickens by Dietary Supplementation of Polyphenols from Shredded, Steam-Exploded Pine Particles

Reducing the detrimental effects of heat stress (HS) in poultry is essential to minimize production losses. The present study evaluates the effects of dietary polyphenols prepared from underutilized wood byproducts on the growth, gut health, and cecal microbiota in broilers subjected to acute heat stress (AHS). One hundred eight one-day-old Indian River broilers were fed with 0%, 0.5%, or 1% polyphenols from shredded, steam-exploded pine particles (PSPP) in their diet. On the 37th day, forty birds were equally distributed among four groups containing either a control diet at thermoneutral temperatures (NT0%) or AHS temperatures with 0% (AHS0%), 0.5% (AHS0.5%), and 1% (AHS1%) PSPP-supplemented diets. The temperature in the NT room was maintained at 21.0 °C, while, in the AHS room, it was increased to 31 °C. AHS negatively influenced performance parameters and increased rectal temperature (RT) in broilers. The AHS0% group showed a higher expression of NOX4, HSP-70, and HSP-90 genes, while the expression was lower in PSPP-supplemented birds. In the jejunum, mRNA expression of SOD was increased in all the birds under AHS compared to NT. The expression of the CLDN1 and ZO2 genes was increased in AHS0%, while that of the ZO1 and MUC2 genes was increased in PSPP-supplemented birds. HS tends to increase TLR2 and TLR4 gene expression in chickens. The significantly modified genera were Bariatricus, Sporobacter, Sporanaerobacter, and Natranaerovirga. Concludingly, AHS negatively influences the performance parameters, RT, stress, gut-health-related genes, and pathogenic penetration, but PSPP supplementation reduces its bad impact by overcoming the stress and gut-health-related genes, increasing favorable bacterial abundance and reducing pathogenic penetration in chickens.

Aescin ameliorates alcohol-induced liver injury. A possible implication of ROS / TNF-alpha / p38MAPK / caspase-3 signaling.

Alcoholic liver disease (ALD) is a commonly known liver disease mediated by prolonged alcohol consumption. Aescin is a triterpene saponin that can manage several conditions, including brain trauma, arthritis, venous congestion, stroke, and thrombophlebitis. Even so, studies illustrating the aescin role in ALD are scarce. Our study explored the potential effect of aescin in ALD in mice. In the current experiment, forty mice were utilized and sorted randomly into four groups: the control group received only vehicles, the alcohol group was given 5% alcohol in drinking water for four weeks, and the aescin-treated groups were given 5% alcohol in drinking water and aescin (10 and 20mg/kg/day) for four weeks, then two doses of 60% alcohol (3g/kg) were given in the 29th and 30th day of the experiment. Our study revealed that aescin ameliorated alcohol-mediated liver damage, including reducing inflammatory cell infiltration and vascular dilatation. The serum concentrations of liver enzymes (ALT and AST) decreased in the aescin-treated groups. The apoptosis and oxidative stress also decreased, and the antioxidant enzyme activities were restored by aescin in alcohol-treated mice. Additionally, aescin decreased ethanol-induced inflammation by downregulating p38 MAPK and tumor necrosis factor-α (TNF-α), suggesting that aescin positively reduces alcohol-caused inflammation and oxidative stress. Consequently, aescin could ameliorate alcohol-induced hepatic damage by targeting the p38 MAPK/TNF-α signalling and could be developed as a novel health product that potentially ameliorates ALD.

Elexacaftor, Tezacaftor and Ivacaftor: immediate efficacy evaluation using home spirometry

Following two weeks of application of the triple combination therapy of Elexacaftor (E), Tezacaftor (T), and Ivacaftor (I) known as ETI, substantial pulmonary improvement in patients with cystic fibrosis is well-documented. However, few detailed data are available on the action of this treatment over the course of these first 14 days. In this prospective study (NCT05599230), 20patients aged≥12years, all of them eligible for ETI, were recruited at the initiation of treatment. Home spirometry (MIR Spirobank®) was performed during the three days preceding the start of treatment and then daily for 14days, while a respiratory symptom score (RSS) was calculated and a log maintained concerning the events experienced by each patient. Mean age (±SD) of the 20patients was 29.4 (±11.1) years, mean FEV1 was 84.2% (±17.7), and the mean BMI z-score was 0.18 (±0.82). Thirteen of them were already on modulators. When compared to the average scores recorded for the three days preceding the start of treatment, FEV1 improvement became significant from the 6th day (D6). After having significantly worsened on D1 (P<0.05), the RSS improved from D6 onwards. The quality of home FEV1 measurements was high (grade A: 81.2%). Under ETI treatment, respiratory benefits were significant from D6. The side effects most commonly perceived by the patients occurred during the first four days of treatment. While daily monitoring of home spirometry could indeed be a valuable tool in follow-up of patients with cystic fibrosis, its administration requires suitable and sustained training.

Efficacy analysis of intratympanic injection of dexamethasone at different concentrations for the treatment of unilateral idiopathic sudden sensorineural hearing loss.

Our study tried to evaluate and compare the therapeutic efficacy of intratympanic injection of dexamethasone (DEX) at different concentrations for the treatment of idiopathic sudden sensorineural hearing loss (ISSNHL). A prospective randomized controlled study was conducted on 203 patients with unilateral ISSNHL. Patients were divided into four groups: the control group (n=47, systemic treatment only), the high-concentration group (n=51, intratympanic injection of DEX 20mg/ml), the medium-concentration group (n=55, 10mg/ml), and the low-concentration group (n=50, 5mg/ml). Pure tone audiometry (PTA) thresholds were measured before treatment and on the 10th day post-treatment, with a follow-up period of 2months. Hearing gains and efficacy rates were compared among the groups. Significant differences were observed in PTA thresholds before and after treatment in all four groups (P<0.05), indicating that both systemic therapy and intratympanic injection were effective. The overall efficacy rate and hearing gain were higher in the intratympanic injection groups compared to the control group, but the differences were not statistically significant. Specifically, the control group achieved an efficacy rate of 57.4% with a hearing gain of 11.25±10.00dB HL. The intratympanic injection groups showed an overall efficacy rate of 67.3% and a hearing gain of 12.50±10.94dB HL, with subgroup results as follows: low-concentration group (62.0%, 12.50±11.56dB HL), medium-concentration group (69.1%, 11.25±13.75dB HL), and high-concentration group (70.6%, 12.50±8.75dB HL). Among patients with severe or profound hearing loss, the high-concentration group exhibited significantly greater hearing gains compared to the control group, while no significant differences were observed in efficacy rates. Combined intratympanic injection of DEX with systemic treatment for SSNHL provides higher hearing gains and efficacy rates compared to systemic treatment alone, though the differences were not statistically significant. In patients with severe or profound hearing loss, intratympanic injection of high-concentration DEX resulted in significantly greater hearing improvement, suggesting a potential therapeutic advantage in this subgroup.

Effect of timber harvesting techniques and slopes on soil respiration of young and mature black pine stands in northwestern Türkiye

Releases of CO2 from forest soils was studied relative to different timber harvesting techniques, slope classes, and stand age. Three timber harvesting techniques (suspended skidding (SS), skidding by using a skidding cone (SC), and cable-pulling (CP)) were used in young and mature black pine stands at three different slopes (0 to 20% – S1, 20% to 33% – S2, and &gt; 33% – S3). Soil respiration measurements were carried out at five-day intervals (1st, 5th, and 10th day) and 6 months later after the timber harvesting techniques. The soil respiration increased on the first day, decreased on the 5th and 10th days, and reached its lowest level on the 6th month. The SC technique in the young stands showed the highest soil respiration value on the slopes indicating that the cone placed on the head of the log during the skidding operations does not deform the soil. In the mature stands, all timber harvesting techniques and the undisturbed sites showed lower values than in the young stands on the S1. On the S2, mean value was higher in the mature stands. In contrast to the young stands, the undisturbed stands had the highest soil respiration.

Quality parameters and shelf life of strawberry (cv. Centenary) fruits as affected by active modified atmosphere packaging

Abstract Modified Atmosphere Packaging (MAP) has gained attention from commercial horticulturalists, in extending the shelf life of bulk and retail packaged fresh produce such as strawberries. Storing strawberries in an atmosphere with optimized gas composition containing carbon dioxide concentration from 15 to 30% and the oxygen concentration from 7.5 to 10% can provide an optimized condition to maintain quality and extend shelf life of strawberries at low temperatures. Therefore this study aimed to investigate the effect of active MAP, three different gas mixes namely: MAP1 (10 O2, 15% CO2, 75% N2); MAP2 (7.5 O2, 17.5% CO2, 75%.N2); MAP3 (30% CO2, 70%.N2) and Passive MAP as a control conventional packaging on changes in gas composition, total soluble solid, colour, electrolyte leakage and density of fresh strawberries during 15 days storage period at 4 °C and 99.9% RH. Strawberries packaged in commercial packaging PET (Polyethylene terephthalate) punnet and PET lidding with macro perforated, a mix of three 8 mm diameter hole with four 5 mm x 15 mm rectangular holes on PET lidding film under normal atmosphere was studied as a control sample. Freshly harvested strawberries were packaged in a PET punnet with PET/PE (Polyethylene terephthalate/Polyethylene) as lidding film using an automatic tray sealer. The packaged strawberries were stored at 4 °C. Storage studies revealed that significantly higher CO2 and negligible O2 concentration in MAP3 compared to the control packaging system. Greater electrolyte leakage was observed for the treatment with higher (30%) CO2 and commercial packaged sample with higher O2 concentration on 13th day of storage (P &amp;lt; 0.05). No significant difference in density value was observed for all the treatments. L* value showed a decreasing trend in strawberries stored in commercial package with higher O2 concentration for 13 days, while it was well maintained in the MAP2 and MAP1 treatments containing 7.5 and 10% O2 up to 15 days.

Efficacy of Shringaveradi Choorna in the Management of Renal Calculi: A Clinical Study with Pre-Post study design

Background: Renal stones are a common health problem, affecting approximately 8-10% of the global population. If left untreated or not treated properly, renal calculi can significantly impair kidney function, and recurrence is also common. Many formulations with asmari bhedaka property have been mentioned in ayurveda literature which are cost effective, devoid of complications and provide wide scope for the successful treatment of mootrasmari. Aim - To evaluate the effect of sringaveradi choorna in reducing the size, signs and symptoms of renal calculi.Materials and methods: the study was a pre and post clinical trial with 23 participants in a single group selected on the basis of inclusion and exclusion criteria. Participants were given 2g sringaveradi choorna twice daily, before food with dadhimanda as anupana for a period of 30 days. Assessment was done on 0th , 15th and 31st day for subjective parameters (pain and tenderness over renal angle, burning micturition ,dysuria) and objective parameters(hematuria, puscells in urine). Renal calculi size and number were assessed before and after the treatment by taking USG(Abd&amp;pelvis). Results: Both subjective and objective parameters were statistically significant with P &lt;0.0001. Size and number of calculi showed significance with p&lt;0.001.Conclusion: On analysing the results it can be concluded that Sringaveradi choorna is effective in reducing the size and number as well as signs and symptoms of renal calculi.

The effect of intrapartum labor induction with oxytocin on maternal depression scoring in early postpartum period: a retrospective cohort study

Aims: Studies showing that synthetic oxytocin (SO) used for induction during labor increases the risk of postpartum maternal depression are increasing day by day in literature. Objective was to investigate the effect of SO administered for induction during labor on the tendency to maternal depression in early postpartum period. Methods: The study encompassed nulliparous women, all of whom delivered at gestational weeks 37 to 41 between 2020 and 2022 and underwent standard postpartum assessments on the 10th day following delivery. Exclusion criteria comprised pregnant individuals with predisposing factors for depression before conception, during gestation, or in the postpartum period. Pregnants who were admitted to delivery room due to labor and gave birth without any induction method were classified as group C (n=137), and who were induced with SO were classified as group O (n=122). Edinburgh Postnatal Depression Scale (EPDS) scores on 0th day and 10th day postpartum of two groups were compared. Additionally, relationship between duration of induction and EPDS scores was analyzed. Results: No statistically significant difference was detected between two groups in terms of age, gestational week, or educational status (p&gt;0.05). No statistically significant difference was detected between the groups in EPDS scores on 0th or 10th day postpartum (p&gt;0.05). There was no significant difference between induction time and EPDS scores on 0th or 10th day postpartum (p&gt;0.05). Conclusion: SO used for labor induction may not primary affect the tendency to postpartum depression in the early period. Further studies in populations without risk factors for depression are needed.

Effects of water-table depth on seed quality as β-glucan content in waxy and non-waxy naked barley grains

ABSTRACT Naked barley (Hordeum vulgare L.) is often planted as a regional specialty crop in the western region of Japan, where there are many hilly and mountainous areas. Water-table depths there are often shallow, due to the inflow of spring melt-water and underground water from the mountains. In this study, the effects of water-table depth on growth and β-glucan content in naked barley were studied by using a lysimeter. The new, waxy-type, naked barley cultivar ‘Fukumifiber’, with high β-glucan content, showed that β-glucan content per grain with same ripeness were decreased by shallower water-table depth, while in the non-waxy cultivar ‘Ichibanboshi’, the effect to β-glucan content per grain was not significant. During ripening, the β-glucan content in both waxy and non-waxy cultivars increased sharply from the 20th to the 30th days after anthesis. In waxy-type cultivar ‘Fukumifiber’, β-glucan further accumulated from the 30th to the 50th days after anthesis, but the accumulation was suppressed as the water-table depth shallowed. While in non-waxy type cultivar ‘Ichibanboshi’, there was no significant increase from the 30th to the 50th days after anthesis and there were no significant differences due to the water-table depth. These results suggest that good drainage may be particularly important before from the 20th to the 30th days after anthesis for all cultivars. Furthermore, for some cultivars similar measures until the 50th day after anthesis may also be important. And even under shallow water-table depth condition, the difference between cultivars that have high β-glucan content or low content was maintained.

MULTIDISCIPLINARY APPROACH TO TREATMENT OF PATIENT WITH BIOPROSTHETIC AORTIC VALVE DYSFUNCTION AND RENAL CELL CARCINOMA WITH TUMOR THROMBUS

HighlightsThis review presents a clinical case of a patient with a rare combination of two life-threatening pathologies whose treatment required high-tech surgery. The effectiveness of a multidisciplinary approach to treatment has been demonstrated. AnnotationAortic stenosis (AS) is the most common heart disease in elderly patients requiring treatment. Malignant neoplasms of the kidneys account for about 3% of all cancers in the Russian Federation, and the formation of tumor thrombi in the renal and inferior vena cava occurs in 4–10% of patients with this pathology. Given the prevalence of aortic stenosis and renal cell carcinoma (RCC), the number of patients with a combination of these two conditions keeps increasing and they are recommended to undergo surgical treatment at multidisciplinary clinical centers. Transcatheter aortic valve implantation (TAVI) and radical nephrectomy with intracorporeal thrombectomy using the DaVinci Si robotic surgical system may be a preferred approach for the treatment of critical AS and RCC for this category of patients.At the multidisciplinary clinical center a patient with dysfunctional bioprosthetic aortic valve implanted in 2010 and NYHA class 3 heart failure with history of endovascular treatment of coronary artery disease, peripheral arterial disease and carotid endarterectomy and concomitant renal cell carcinoma with tumor thrombosis of the renal vein received surgical treatment in 2 stages: TAVI – the first stage, and robot-assisted right-sided nephrectomy and thrombectomy from the renal vein – the second stage. The patient was discharged from the Center on the 7th day after TAVI and on the 6th day after surgery for renal cancer. Six months after the procedure, there was a decrease in the functional class of heart failure, satisfactory function of the transcatheter aortic valve bioprosthesis, decrease in the size of the left ventricle, decrease in the brain natriuretic peptide, and the absence of cancer progression.

EFFECT OF AGNIKARMA IN AMSAMARMA ABHIGHATA - TRAUMATIC PERI-ARTHRITIS OF THE SHOULDER JOINT- A CASE REPORT

Periarthritis of the shoulder joint is a condition characterised by disabling pain, inflammation, stiffness, and restriction of both active and passive movements of the shoulder joint. Among the factors suggested for the cause of the disease, trauma is found to be one of them. Traumatic periarthritis of the shoulder joint may be correlated with Amsamarma Abhighata due to similarity in clinical features such as Bahukriyaharam and Stabdabahutha. Acharya Susrutha mentioned that Agnikarma is effective in Ruja pertaining to Sira, Snayu, Sandhi and Asthigata Vikara. Amsamarma is a Snayu Marma and Acharya Susrutha has mentioned Dahanopakarana like Kshoudra, Guda, Sneha for Agnikarma in Sira, Snayu, Sandhi, Asthi gata Vikara. In this study, a female patient 60 years was treated with Agnikarma to assess the effect of Agnikarma with Tila Taila in Amsamarma Abhighata - Traumatic periarthritis of shoulder joint. Agnikarma has properties like Vatakaphahara, Ushna, Sookshma, and Ashukari Guna, which help relieve pain and stiffness instantly. Snigdha dravyas have deep heat penetration capacity with a greater latent heat period. It is helpful in musculoskeletal disorders and can be done on an OPD basis. The assessment was done on subjective parameter, pain and objective parameter like range of movements(Abduction). The patient was assessed before and after treatment. Follow-up was done on the 7th, 15th and 30th day for 1 month.