60Co Gamma Rays Research Articles

Silicon Solar Cells for Post-Detonation Monitoring and Gamma-Radiation Effects

The feasibility of using solar cells for post-detonation monitoring, and more broadly, gamma-ray monitoring, is evaluated using Monte Carlo simulations and experiments in this work. We measured the short-circuit current Isc response of commercial silicon (Si) solar cells to 137Cs and 60Co gamma rays. A clear response of both mono- and polycrystalline Si solar cells to 137Cs and 60Co gamma rays was obtained in good agreement with the simulations. Radiation effects in solar cells due to accumulated gamma-ray dose were noticed as the drop in Isc and open-circuit voltage Voc. The atomic displacement cross section of the produced secondary fast electrons and nonionizing energy loss (NIEL) concepts were revisited to understand the principal gamma-radiation damage mechanism in solar cell devices. Analytical computations of and NIEL of electrons convoluted with simulated Compton electron distributions in Si enabled a fundamental understanding of the gamma-radiation effects and recovery mechanism in solar cells, further supporting the experimental results. Different from the ionization effects in the polymer and glass layers of a solar cell/panel, displacement damage in the Si p-n layer from gamma rays or fast electrons is much less than that from massive particles, which directly affects the charge collection performance fundamental to solar cell operation.

Changes in patient peripheral blood cell microRNAs after total body irradiation during hematopoietic stem cell transplantation.

BackgroundIonizing radiation exposure is a great threat to human health. MicroRNAs (miRNAs) have been shown to play an important role in radiation-induced biological effects. Here, we investigated plasma miRNA expression changes and differentially expressed miRNAs in radiotherapy patients exposed to cobalt-60 (60Co) gamma rays to provide an experimental basis for human plasma miRNAs as an estimation indicator for ionizing radiation injury.MethodsSix patients with acute lymphoblastic leukemia (ALL) received continuous 5 gray (Gy) total body irradiation (TBI) twice. At 12 hours after irradiation, miRNA microarray was applied to screen for differentially expressed miRNAs, with some miRNAs confirmed by real-time polymerase chain reaction (RT-PCR) assay. Bioinformatic analysis was carried out to identify the relevant target genes and biological function of the differentially expressed miRNAs.ResultsAfter radiotherapy patients were exposed to 5 Gy gamma radiation, the expression of 9 plasma miRNAs was significantly upregulated, and the expression of 2 miRNAs was downregulated. After irradiation with 10 Gy gamma radiation, the blood plasma of radiotherapy patients contained 18 differentially expressed miRNAs, of which 17 were upregulated and 1 was downregulated (P<0.05). The expression of miR-4532, miR-4634, miR-4655-5p, miR-4763-3p, miR-4785, miR-6087, miR-6850-5p, and miR-6869-5p were significantly upregulated in both the 5-Gy and 10-Gy dose groups, showing a certain dose-response relationship. The RT-PCR results were consistent with the findings of high-throughput sequencing. In addition, the target genes of the differentially expressed miRNAs were mainly involved in RNA transcription and DNA damage. Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis revealed that these miRNAs participated in phosphoinositide 3-kinase/protein kinase B (PI3K/AKT), Ras, mitogen-activated protein kinase (MAPK), and other signaling pathways.ConclusionsThe expression of differential plasma miRNAs of radiotherapy patients was associated with irradiation injury and showed a certain dose-effect relationship. These differentially coexpressed plasma miRNAs could be used as an early indicator for estimating radiation injury.

Exogenous melatonin ameliorates ionizing radiation-induced damage by modulating growth, osmotic adjustment and photosynthetic capacity in wheat seedlings

As a multifunctional signal molecule, melatonin (N-acetyl-5-methoxytryptamine) plays many important roles in the regulation of plant growth and development. The effect of melatonin application on enhancing plant stress tolerance has been widely reported, but the ameliorative effect of exogenous melatonin treatment on plants exposed to ionization stress is still unknown. This study investigated the ameliorative effects of two types of melatonin treatment, pre-sowing priming (prMel) and application during growth (ptMel), in wheat (Triticum aestivum L.) seedlings exposed to different radiation doses (100, 200, 300 and 400 Gy) of radioactive cobalt (60Co) gamma rays as dry seeds. The growth parameters, photosynthetic pigments, chlorophyll fluorescence, osmotic potential with soluble sugars, fructans and proline contents were then examined. The results indicated that high doses of ionizing radiation (IR) led to decreases in plant growth, pigment contents, chlorophyll fluorescence ratios and osmotic potential. However, soluble sugar, fructan and proline contents increased under IR stress conditions. Both melatonin applications, but particularly prMel, enhanced the morphological parameters, preserved the photosynthetic machinery and regulated the osmotic adjustment of IR-stressed wheat seedlings. Taken together, the findings show that exogenously applied melatonin, particularly prMel, play a significant role in alleviating IR stress in wheat seedlings.

Effect of Opuntia elatior on Alteration in Glutamic Oxaloacetic Transaminase activity induced by Gamma Radiation in Swiss Albino Mice

When mice are exposed to gamma radiation, all the body systems are affected adversely. Liver enzymes which are responsible for removal of toxicity created by irradiation increases in the body. Several herbs are known to reduce this toxicity. Opuntia elatior (OE) ,a cactus, was tested in this study for its activity to remove toxins induced by whole body exposure to Co60 gamma rays (6Gy).A moderate dose of OE (10 mg/kg body weight) was given orally to mice, before whole body irradiation and GOT activity was studied in their kidney at 1,3,5,10,15 and 30 post treatment days . Whole body gamma ray exposure increases GOT activity in the kidneys also. In OE pretreated animals this increase was significantly lesser and early recovery was observed. Two types of extracts were used in this study (ethanolic and acetonic). Both were able to reduce gamma radiation induced increase significantly and almost equally. As OE cladode extracts are quite cheap and produce no side effects(OE cladodes are used as food), it may be used to prevent unwanted toxic effects of gamma radiation exposure.&#x0D;

Research on the gamma irradiation decomposition products of 2,6-diamino-3,5-dinitropyrazine-1-oxide (LLM-105)

In this work, 60Co gamma-ray source was used to irradiate LLM-105 at room temperature with the radiation doses of 100, 300, 500, 696, and 900 kGy under vacuum. High-performance liquid chromatography coupled with electrospray mass spectrometry (HPLC–MS) and 1H nuclear magnetic resonance (NMR) demonstrated the main radiolysis product in gamma-irradiated LLM-105 was 2,6-diamino-3,5-dinitropyrazine (ANPZ). The relationship between ANPZ content and irradiation dose indicated that LLM-105 was a stable explosive under gamma irradiation. The sensitivity is not expected to change based on the chemical analyses performed on irradiated LLM-105. The radiation decomposition mechanism of LLM-105 was clarified by Atomic dipole moment corrected Hirshfeld (ADCH) atomic charges and electronic localization function (ELF).

Gamma-ray engineered surface defects on zinc oxide nanorods towards enhanced NO2 gas sensing performance at room temperature

Gamma-ray irradiation is a fast and efficient technique that can constructively create various defects in a controlled manner to tune materials’ functionality. In this work, zinc oxide nanorods are hydrothermally grown over flexible polyethylene terephthalate paper substrate and then irradiated with 60Co gamma-ray source to create various defects for possible enrichment towards NO2 gas sensing. The sensors irradiated with gamma-ray show enhanced NO2 responses of 120%, 160%, and 185% for 500 ppb concentration at the irradiation doses of 1.5 kGy, 4.5 kGy, and 6 kGy, respectively, as compared to the pristine sensor response of 80%. Maximum response of 312% is achieved at the irradiation dose of 6 kGy for 1500 ppb of NO2. Overall, this study confirms that the enhanced oxygen vacancies, defect concentration, and increased grain boundaries, caused by gamma-ray irradiation can eventually achieve more than two times better efficiency towards NO2 detection at room temperature (25 ℃) on a flexible based NO2 sensor.

The Effectiveness of Bi2O3 Apron on Attenuation of X-Ray and Gamma Ray by MCNPX Simulation

Apron is one of the radiation shields, generally the apron used in radiodiagnostic installations is made of lead. However, lead aprons have disadvantages such as toxicity and relatively heavy weight. Therefore, it is necessary to innovate apron materials that are better than lead. This research was conducted by modelling an apron made of Bi2O3 with polyvinyl chloride as a coating and Pb as a standard apron. The used energies are 60, 90 &amp; 120 keV for X-rays, 662 keV for Cs-137 (gamma ray), and 1173 and 1333 keV for Co-60 (gamma rays). The thickness of the radiation barrier is from 0.5 to 4 cm, and the distance of radiation source and detector is 45 cm. Bi2O3 apron geometry modelling was carried out using the MCNPX program. The modelling results show that at X-ray energy of 60 keV, the Bi2O3 apron can replace the Pb apron at a thickness of 1 cm to 4 cm. While at X-ray energy of 90 keV, the Bi2O3 apron can replace the Pb apron at a thickness of 2,5 cm to 4 cm, and at X-ray energy of 120 keV Bi2O3 apron can replace the Pb apron at a thickness of 1,5 cm to 4 cm. From these results it can be seen that the technology apron made from Bi2O3 with polyvinyl chloride as a coating with a certain thickness can be a non-lead radiation shielding material that is more environmentally friendly. But at the gamma energy of Cs-137 of 662 keV, Co-60 of 1173 keV and 1333 keV, Bi2O3 apron cannot replace Pb apron because the absorption of gamma rays is smaller than the absorption of X-rays. Keywords: Bi2O3 apron, X-ray attenuation, Gamma ray attenuation, MCNPX .

Structural and defect changes in black carbon charcoal irradiated with gamma ray

This study investigates the use of black carbon charcoal as passive radiation dosimetry, offering low dependence on photon energy and near soft tissue effective atomic number with state-of-the-art techniques. Regression analyses have now been conducted using graphite manufactured commercially in the form of charcoal from three different types: mangrove, coconut, and green charcoal recycled from sawdust, working with photon-mediated interactions at radiotherapy dose levels. Explorations of changes in Raman spectroscopic characteristics, and photoluminescence dose dependence have been performed with a focus on the relationship between absorbed radiation energy and induced material changes, using a 60Co gamma-ray source doses ranging from 0 to 10 Gy. Raman spectroscopy has established to be an effective method for exploring defects in carbon-based materials due to its high sensitivity, most commonly focusing on the use of ID/IG parameter. While photoluminescence analysis will provide information on electronic properties and the band gap energy. The crystal structure of the black charcoal samples was characterised using X-ray diffractometry, with the goal of determining the degree of structural order, atomic spacing, and lattice constants of the various irradiated charcoal samples, supported by crystallite size assessments. The findings of this study could pave the way for a low-cost yet highly effective system for studying radiation-induced changes in carbon, as well as offering a viable alternative to current commercial dosimeters, well suited to applications in radiotherapy.

Gamma Radiation (60Co) Induces Mutation during In Vitro Multiplication of Vanilla (Vanilla planifolia Jacks. ex Andrews)

In vitro mutagenesis is an alternative to induce genetic variation in vanilla (Vanilla planifolia Jacks. ex Andrews), which is characterized by low genetic diversity. The objective of this study was to induce somaclonal variation in V. planifolia by gamma radiation and detect it using inter-simple sequence repeat (ISSR) molecular markers. Shoots previously established in vitro were multiplied in Murashige and Skoog culture medium supplemented with 2 mg·L−1 BAP (6-benzylaminopurine). Explants were irradiated with different doses (0, 20, 40, 60, 80 and 100 Gy) of 60Co gamma rays. Survival percentage, number of shoots per explant, shoot length, number of leaves per shoot, and lethal dose (LD50) were recorded after 60 d of culture. For molecular analysis, ten shoots were used for each dose and the donor plant as a control. Eight ISSR primers were selected, and 43 fragments were obtained. The percentage of polymorphism (% P) was estimated. A dendrogram based on Jaccard’s coefficient and the neighbor joining clustering method was obtained. Results showed a hormetic effect on the explants, promoting development at low dose (20 Gy) and inhibition and death at high doses (60–100 Gy). The LD50 was observed at the 60 Gy. Primers UBC-808, UBC-836 and UBC-840 showed the highest % P, with 42.6%, 34.7% and 28.7%, respectively. Genetic distance analysis showed that treatments without irradiation and with irradiation presented somaclonal variation. The use of gamma rays during in vitro culture is an alternative to broaden genetic diversity for vanilla breeding.

Comparison of Total Ionizing Dose Effects in 22-nm and 28-nm FD SOI Technologies

Total ionizing dose (TID) effects from Co-60 gamma ray and heavy ion irradiation were studied at the 22-nm FD SOI technology node and compared with the testing results from the 28-nm FD SOI technology. Ring oscillators (RO) designed with inverters, NAND2, and NOR2 gates were used to observe the output frequency drift and current draw. Experimental results show a noticeable increased device current draw and decreases in RO frequencies where NOR2 ROs have the most degradation. As well, the functionality of a 256 kb SRAM block and shift-register chains were evaluated during C0-60 irradiation. SRAM functionality deteriorated at 325 krad(Si) of the total dosage, while the FF chains remained functional up to 1 Mrad(Si). Overall, the 22-nm FD SOI results show better resilience to TID effects compared to the 28-nm FD SOI technology node.

60Co gamma ray induced mutants of cowpea and assessment of genetic variability by SCoT marker

Mutagenesis is a well-known technique for introducing new variants into crop plants. In the present study, M2 populations were generated in the cowpea (Vigna unguiculata (L.) Walp.) variety CO7 using gamma irradiation. The M2 progeny were used to investigate the effectiveness of the gamma irradiation doses and examined for the agronomic traits. The genomic variation present in the mutants and their parents was analysed using five SCoT markers. Marker analysis revealed a total of 87 amplicons and among these, 20 amplicons showed polymorphism. The highest numbers of amplicons were observed at SCoT10 (39), while the lowest number of amplicons was produced by SCoT09 (07). The percentage of polymorphism ranged from 18.18% to 28.57%, with an average of 21.12%. Polymorphic information content (PIC) values ranged from 0.197 to 0.345. Analysis of Molecular Variation (AMOVA) showed 12% and 88% between the genotypes and within the genotypes respectively. The constructions of 4 clusters were identified through Unweighted Pair Group Method with Arithmetic Mean (UPGMA) dendrogram tree based on the genetic distance deduced from SCoT marker analysis. Analysis of the genetic relatedness between parent and mutants through Principal Coordinate Analysis (PCoA) revealed two main groups. The present study concludes that the genetic variability induced by gamma irradiation and inherited in the next generations. This research investigation supports that gamma irradiation alters the growth and yield traits, which is helpful for generating the cowpea improvement.

Optical-electrical characteristics of Al/Gd2O3/(CZ-pSi)/Al diodes under gamma ray irradiation

This article aimed to report the results on the properties of Gd2O3/(CZ-pSi) diodes under various gamma ray irradiation dose rates (3, 6, 12, 18, 24, 30, 36, 42, 50 Gy). n-type Gd2O3 films were deposited on a p-type Czochralski-grown monocrystalline silicon (CZ-pSi) wafers by pulsed laser deposition (PLD) technique with varying building laser energies (500, 600, 700, 800, 900 mJ) at room temperature and pressure (3.3 × 10−2 Pa). Amorphous structures were perceived for all films by XRD. Band gaps of the films were modified by controlling the amount of Gd2O3 on CZ-pSi wafers by laser power during deposition and determined to be between 5.30 and 5.75 eV. The Gd2O3/(CZ-pSi) films were irradiated by a Co-60 gamma-ray source system. The current and voltage (I-V) measurements, before and after irradiation (0–50 Gy), indicated that the current decreases toward zero with increasing doses. Capacitance and voltage (C-V) measurements at frequencies of 10–1000 kHz before and after irradiation showed a shift towards the right side of the pre-irradiation curves for each film. The shift was observed in both I-V and C-V curves with increasing radiation doses at 100 kHz as the average of the frequencies used. Contrary to the decrease in current curves, there was an upward increase in capacitance curves of all films with increasing radiation dose. The best build-in potentials of 0.1 V (800 mJ), 1.2 V (500 mJ) and 1.4 V (600 mJ) were obtained for the laser deposition powers of the Gd2O3/(CZ-pSi) diodes. These results are promising for diodes to be used in the radiation detection applications such as in neutron and alpha particles detectors.

The effect of treatment with HEMA and gamma irradiation on the starch:PVA films studied by differential scanning calorimetry and thermogravimetry

Basic studies were conducted dealing with the influence of treatment with hydroxyethyl methacrylate (HEMA) supported by gamma irradiation on physicochemical properties of the starch:PVA films (45:55, glycerol content of 30 wt%). The films were placed in a 10% monomer solution, irradiated with 60Co gamma rays applying a dose of 25 kGy, then heated at 45 °C for 2.5 h and subjected to a prolonged contact with the solution. Simultaneously, the reference sample subjected to the same treatment without action of HEMA, and additionally the samples irradiated in monomer solution without heating step, were prepared. FTIR spectroscopy has shown an effective grafting of HEMA. The differences were found between the course of thermal degradation occurring in HEMA treated sample and the reference sample both in nitrogen and in air. Data analysis indicated formation of the fractions characterized by an increased as well as by a decreased thermal stability. Simultaneously, DSC enabled to discover the differences between melting, crystallization, glass transition and structural relaxation phenomena taking place in the monomer treated and the non-treated samples. The changes were referred to a decrease in the crystallinity accompanied by an increase in the ordering in the amorphous phase. TGA and DSC data were related to chemical changes shown by FTIR spectroscopy and by gel content data and discussed in terms of degradation as well as grafting of HEMA onto the polymer matrix. The increased temperature of the treatment raised degree of the occurring changes.

Gamma radiation-induced photoluminescence in calcium phosphate glass and inhibiting effect from copper

The influence of gamma-ray irradiation on the optical properties of 50P2O5–30CaO–20Na2O glass and quaternary ones prepared with [CuO]/[Na2O] molar ratios of 0.1, 0.7 and 1.5 at fixed [P2O5]/[CaO] is reported. The glasses prepared by melting were subjected to 100 kGy Co-60 gamma ray irradiation and studied by optical transmission and photoluminescence (PL) spectroscopy. The gamma-irradiated 50P2O5–30CaO–20Na2O ternary developed a ruby-red color and showed absorption features around 425 and 500 nm attributed to oxygen hole centers. The quaternary with [CuO]/[Na2O] molar ratio of 0.1 merely showed a weak absorption feature around 425 nm, whereas the highest molar ratios did not show significant differences relative to the pristine. Analyses of band gap and Urbach energies were made to aid in evaluating radiation-induced changes. Further, the irradiated copper-free glass exhibited a distinct blue luminescence band around 415 nm (not observed prior). It was most favorably excited in the UV at 265 nm, suggesting a phosphorus electron center at its origin. Its decay kinetics appeared relatively slow with a first-order exponential decay with lifetime of 4412 (±4) μs. Conversely, such emission was not detected for the copper-containing glasses, which instead exhibited PL properties comparable to the unirradiated counterparts. Finally, the effect of heat treatment after the gamma ray exposure was evaluated. The radiation-induced color centers showed a bleaching effect whereas the new PL vanished thus supporting the defects-related origin.

Biological Effect of Gamma Rays According to Exposure Time on Germination and Plant Growth in Wheat

Gamma rays as a type of ionizing radiation constitute a physical mutagen that induces mutations and could be effectively used in plant breeding. To compare the effects of gamma and ionizing irradiation according to exposure time in common wheat (Keumgang, IT 213100), seeds were exposed to 60Co gamma rays at different dose rates. To evaluate the amount of free radical content, we used electron spin resonance spectroscopy. Significantly more free radicals were generated in the case of long-term compared with short-term gamma-ray exposure at the same dose of radiation. Under short-term exposure, shoot and root lengths were slightly reduced compared with those of the controls, whereas long-term exposure caused severe growth inhibition. The expression of antioxidant-related and DNA-repair-related genes was significantly decreased under long-term gamma-ray exposure. Long-term exposure caused higher radiosensitivity than short-term exposure. The results of this study could help plant breeders select an effective mutagenic induction dose rate in wheat.

Statistical analysis of the periodic intermediate checks results on the standards used for calibrations of ionizing radiation dosimeters in a 60Co gamma ray beam

Periodic intermediate checks on the equipment used for calibration activity accredited for the conformity with the international norm ISO/IEC 17025 are an essential issue for monitoring the validity of calibration laboratory's results. These checks should be carried out according to a procedure defined by each accredited laboratory. The results of these checks should not exceed the acceptance criteria established in the laboratory and statistical techniques shall be applied to the reviewing of the results. In this work, a simple method of carrying out intermediate checks on working standards used for calibrations of ionizing radiation dosimeters in a 60Co gamma ray beam was presented. Moreover, the statistical analysis of obtained results was presented. This analysis allowed for a reliable inference regarding the operation of the working standards used to calibrate the customer's devices. The presented methods can be used as a guidance in order to conform to the ISO/IEC 17025 requirements for carrying out and reviewing of periodic intermediate checks results in the area of calibrations of ionizing radiation dosimeters for external beam radiotherapy.

Effect of Gamma Radiation on Genetic Diversity Shallots (Allium ascalonicum L.) M4 Bauji Variety For Varieties Improvement

Shallots (Allium ascalonicum L.) is a horticultural commodity that has high economic value in Indonesia. Efforts to increase the productivity and quality of shallots will continue to be carried out through plant breeding programs. One of the efforts is through mutation breeding with the aim of improving the Bauji variety so that it has high yields, good quality and is resistant to major pests and diseases. One of the factors that play a role in increasing shallot production is superior varieties. For the assembly of superior varieties, it is necessary to expand genetic diversity, one of which can be done by radiation mutation. With radiation mutations, new genetic diversity is created so that it provides more opportunities for selection. This research was carried out using the single plant method and using the T-test, with the yield of shallots with radiation doses without radiation doses or 0 Gy, 1 Gy, 2 Gy, 3 Gy, 4 Gy, 5 Gy, 6 Gy. Observation parameters included plant length, number of leaves, number of tillers, tuber diameter, and tuber weight. This study aims to obtain the value of genetic diversity and heritability on the agronomic characters of shallots of bauji variety with 60Co gamma-ray radiation treatment. Gamma-ray irradiation of 60Co affects the growth and yield characteristics of the fourth generation Bauji variety of shallots. Irradiation treatment with a dose of 4 Gy (B4) had better results on the parameters of plant length, number of leaves, tuber diameter, and number of tillers than control plants or without irradiation.

Total Ionizing Dose Effects on Read Noise of MLC 3-D NAND Memories

This article analyzes the total ionizing dose (TID) effects on noise characteristics of commercial multi-level-cell (MLC) 3-D NAND memory technology during the read operation. The chips were exposed to a Co-60 gamma-ray source for up to 100 krad(Si) of TID. We find that the number of noisy cells in the irradiated chip increases with TID. Bit-flip noise was more dominant for cells in an erased state during irradiation compared to programmed cells.

Total Ionizing Dose Effects on Long-Term Data Retention Characteristics of Commercial 3-D NAND Memories

This article evaluates the data retention characteristics of irradiated multilevel-cell (MLC) 3-D NAND flash memories. We irradiated the memory chips by a Co-60 gamma-ray source for up to 50 krad(Si) and then wrote a random data pattern on the irradiated chips to find their retention characteristics. The experimental results show that the data retention property of the irradiated chips is significantly degraded when compared to the un-irradiated ones. We evaluated two independent strategies to improve the data retention characteristics of the irradiated chips. The first method involves high-temperature annealing of the irradiated chips, while the second method suggests preprogramming the memory modules before deploying them into radiation-prone environments.

Induced variations by gamma radiation and EMS on the agronomic traits, essential oil yield with its quality and their exploitation in Java citronella (Cymbopogon winterianus Jowitt)

Purpose The slips of Cymbopogon winterianus (Java citronella) are used to propagate this industrially important crop. It does not have a mating system that allows it to create new variability or develop superior progenies, which could be addressed by using induced mutation caused by physical or chemical agents. Physical treatments, rather than chemical treatments, are believed to be the most incredible alternative for mutation breeding. As a result, this study aims to assess the effects of gamma radiation and EMS treatment on the development of better C. winterianus progenies. Materials and methods The three superior lines, i.e. JC-62 (IC-0627007), JC-55 (IC-0627000), and JC-71 (IC-0627018) based on high herbage, essential oil yield and citronellal content were selected through clonal selection during three-year evaluation. These were then irradiated with 60Co gamma rays (50 Gy, 100 Gy, 150 Gy, 200 Gy, 250 Gy, and 300 Gy) and treated with EMS dose (0.1, 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9 and 1%) separately. Ten plants of each dose were mutated and planted in the experimental field to obtain the MV2 progenies. After 4 months, the plants were harvested, and the slips were prepared and planted to get the MV3 and subsequently MV4 progenies. All morphological and essential oil data have been recorded and analyzed statistically. The essential oil was extracted through the Clevenger apparatus for all the progenies, followed by GC and GC-MS analysis. Results The morpho-chemical study of the 112 healthy mutants has led to the identification of six superior progenies in MV4 progenies, M17, M63, M77, M179, M233, and M392. In terms of the economic yield, it was observed that mutant M77 treated with 0.20% EMS dose increased the herbage yield and citronellal content tremendously. In contrast, the progeny M17 irradiated with γ-100Gy increased essential oil yield from 1.32% to 1.50%. The EMS treatment at 0.10% and 0.20% doses showed an increasing trend in plant height, leaf length, tillers/bush, leaves/bush, herbage yield, and citronellal content compared to the base variety. The LD50 value of C. winterianus for gamma radiation and EMS treatment was averaged at 173 Gy and 0.72%, respectively. Conclusion In C. winterianus, both gamma rays and EMS treatment successfully created superior progenies with desired characteristics. The 0.20 percent EMS treatment significantly improved economic features, including herbage yield and citronellal content. Simultaneously, gamma radiation at 100 Gy produced the highest essential oil yield, which is a highly desirable characteristic in C. winterianus breeding.