4th Stage Research Articles

Gene Expression of Monoterpene Synthases Is Affected Rhythmically during the Day in Lavandula angustifolia Flowers

Lavender essential oil (EO) is widely used for medicinal purposes. The significant monoterpenes’ abundance of linalool and linalool acetate accounts for more than 50% of lavender EO compounds. Monoterpenes synthesis differs throughout plant development as a result of the differential gene expression patterns in distinct cell types. Previously, we have reported that the chemical composition of Lavandula angustifolia cv. etherio EO was affected by diurnal harvest time. The aim of this was to evaluate if the gene expression of lavender monoterpenes synthases is altered during the day length and correlated with the accumulation of the major components of lavender EO. The relative expression of linalool synthase (LaLINS), limonene synthase (LaLIMS) and terpene synthase-like (LaTPS-l) was recorded in flowers at the 3rd to 5th stage every 3 h during two consecutive days using quantitative real-time PCR. The composition of the lavender EO was also monitored during the day length using GC-MS analysis. Our results indicate that the expression of genes involved in the synthesis of lavender EO, including linalool and limonene synthases, is accompanied by oscillations, picking at mid-day and leading to linalool acetate accumulation in the afternoon. In conclusion, the monoterpenes synthase expression in lavender flowers is rhythmically affected during the day, leading to a higher accumulation of EO compounds in the afternoon. These results will be helpful to monitor the biosynthesis of lavender EO to ensure a high-quality product. Furthermore, the outcome of this study will be useful for breeding programs in the lavender field to modulate the biosynthesis of linalool and linalool acetate during the flowering harvest period.

Evaluation of Bed Disinfectants on Rearing Performance of Multivoltine Hybrid Mulberry Silkworm [NISTARI × (SK6×SK7)], in Kishanganj District, Bihar, India

The silkworm, (B. mori L). is highly susceptible to pathogens such as protozoa, virus, bacteria and fungus. To evaluate the various bed disinfectant viz, Vijetha, Sericilin, Labex, and Amla compare with untreated control for their suitability and standardization for the management of diseases during silkworm rearing. The rearing of silkworm multivoltine hybrid race [NISTARI × (SK6×SK7)] was carried out in the centre. In the present study the larvae treated with Labex @ 5 g/sq. ft. ½ an hour before resuming feeding had shortest (20.00 days) larval duration. Larvae weight higher (31.24 g) respectively at late 5th instar stage. Larval mortality was observed (25.93%) Effective rate of rearing (64.08%) and significantly lowest disease incidence (26.06%) was recorded.

The prognostic value of 11th Japanese classification and 8th AJCC staging systems in Chinese patients with esophageal squamous cell carcinoma

BackgroundTwo staging systems, the 8th staging system by the American Joint Committee on Cancer (AJCC) and the 11th Japanese classification by Japan Esophageal Society (JES), are currently applied in the clinic for predicting the prognosis of patients with esophageal squamous cell carcinoma (ESCC). The differences between the two staging systems have been widely researched. However, little studies focus on the differences in specific staging between the two systems. Therefore, we aimed to compare the performance of different staging in predicting overall survival (OS) of Chinese patients with ESCC.MethodsThis retrospective study included 268 patients who underwent radical esophagectomy and mediastinal lymph node dissection for ESCC between January 2008 and December 2013. Patients were staged by the 8th AJCC and 11th JES staging systems. OS was estimated using the Kaplan–Meier method and compared between N stages and between stage groupings using the log-rank test. Cox proportional hazards regression analysis was performed to identify factors independently related to outcome. Further, we compared the concordance indexes (C-indexes) of the two staging systems.ResultsThe mean age was 61.25 ± 7.056 years, median follow-up was 44.82 months, and 5-year OS rate was 47%. The OS was well predicted by the 8th AJCC N staging (P < 0.001) and the 11th JES N staging (P < 0.001), with a c-index of 0.638 (95% CI: 0.592–0.683) for AJCC N staging and 0.627 (95% CI: 0.583–0.670) for JES N staging (P = 0.13). In addition, the OS was also well predicted by stage groupings of the 8th AJCC (P < 0.001) and the 11th JES systems (P < 0.001), with a c-index of 0.658 (95% CI: 0.616–0.699) for 8th AJCC stage grouping and 0.629 (95% CI: 0.589–0.668) for the11th JES stage grouping (P = 0.211).ConclusionsThe prognostic effect of 11th JES staging system is comparable with that of AJCC 8th staging system for patients with ESCC. Therefore, both systems are applicable to clinical practice.

Insights on the Nutritional Profiling of Cantaloupe (Cucumis melo L.) via 1-Naphthalene Acetic Acid.

The nutritional components of cantaloupe, including vitamins, minerals, antioxidants, and dietary fiber, contribute to overall health, improved immunity, hydration, and protection against chronic diseases. This study was conducted to investigate the influence of different concentrations (0 (control), 100, 150, and 200 ppm) of 1-naphthalene acetic acid (1-NAA) on the nutritional components of the cantaloupe (Cucumis melo L. Var. Super White Honey). All the studied treatments were applied twice at the 2nd and 4th leaf stages. The applied concentrations of 1-NAA significantly improved the sex expression and fruit yield attributes. Different nutritional components like proximate contents, minerals, vitamins, selected fatty acids, and amino acids were analyzed. The results showed that the maximum moisture content, proteins, carbohydrates, ash, and energy were recorded with 100 ppm. The higher lipids were recorded during the supplementation of 150 ppm. Significantly greater fibers were recorded using 200 ppm. Regarding minerals, 100 ppm was found to be the best as it increased calcium (Ca), magnesium (Mg), potassium (K), sodium (Na), phosphorous (P), manganese (Mn), copper (Cu), iron (Fe), and zinc (Zn). Vitamins were also found to be the maximum with 100 ppm, including vitamin A, vitamin B, vitamin C, vitamin D, vitamin E, and vitamin K. Total selected fatty acids and amino acids were also found significantly greater in the fruits administered 100 ppm.

Effect Analysis of Hydrogen Peroxide Using Hyperspectral Reflectance in Sorghum [Sorghum bicolor (L.) Moench] under Drought Stress.

Due to global climate change, adverse environments like drought in agricultural production are occurring frequently, increasing the need for research to ensure stable crop production. This study was conducted to determine the effect of artificial hydrogen peroxide treatment on sorghum growth to induce stress resistance in drought conditions. Hyperspectral analysis was performed to rapidly find out the effects of drought and hydrogen peroxide treatment to estimate the physiological parameters of plants related to drought and calculate the vegetation indices through PLS analysis based on hyperspectral data. The partial least squares (PLS) analysis collected chlorophyll fluorescence variables, photosynthetic parameters, leaf water potential, and hyperspectral reflectance during the stem elongation and booting stage. To find out the effect of hydrogen peroxide treatment in sorghum plants grown under 90% and 60% of field capacity in greenhouses, growth and hyperspectral reflectance were measured on the 10th and 20th days after foliar application of H2O2 at 30 mM from 1st to 5th leaf stage. The PLS analysis shows that the maximum variable fluorescence of the dark-adapted leaves was the most predictable model with R2 = 0.76, and the estimation model suitability gradually increased with O (R2 = 0.51), J (R2 = 0.73), and P (R2 = 0.75) among OJIP parameters of chlorophyll fluorescence analysis. However, the estimation suitability of predictions for moisture-related traits, vapor pressure deficit (VPD, R2 = 0.18), and leaf water potential (R2 = 0.15) using hyperspectral data was low. The hyperspectral reflectance was 10% higher at 20 days after treatment (DAT) and 3% at 20 DAT than the non-treatment in the far red and infra-red light regions under drought conditions. Vogelmann red edge index (VOG REI) 1, chlorophyll index red edge (CIR), and red-edge normalized difference vegetation index (RE-NDVI) efficiently reflected moisture stress among the vegetation indices. Photochemical reflectance index (PRI) can be used as an indicator for early diagnosis of drought stress because hydrogen peroxide treatment showed higher values than untreated in the early stages of drought damage.

Fault Striae Analysis and Paleostress Reconstruction of the Northern Tectonic Province (Egyptian Nubian Shield): Insights into the Brittle Deformation History of the Northern East African Orogen

The Egyptian Nubian Shield (ENS), the northwestern continuation of the East African Orogen (EAO), comprises a variety of three lithologically and structurally different tectonic provinces—southern compressional-, central transpressional/wrench-, and northern extensional- provinces. The extensional tectonic province (ETP) extends from Qena‒Safaga shear belt up to the northern tip of the ENS. Several lines of evidence indicate extensional regime in the ETP, such as mantle delamination-induced post-orogenic magmatism and bimodal Dokhan Volcanics, together with the pervasive E‒W dyke swarms and the E‒W (to ENE‒WSW) oriented extensional fractures. The present work is devoted to carry out the fault striae analysis and paleostress reconstruction of the northern tectonic province (ENS) in attempt to add more contribution to the brittle deformation history of the northern EAO. Field measurements collected from six areas (I‒VI) have been processed using Win-Tensor Software. Analysis of fault-slip data revealed four paleostress tensor stages (groups or regimes) that prevailed in the ENS. The stress states and related tectonic regimes are characterized by the stress regime index R' and the horizontal stress axes as follows: 1st stage: transpression (R' = 1.91), E‒W compression (SHmax = N090°‒270° E); 2nd stage: transtension (R' = 1.16), N‒S compression (SHmax = 174°‒354° E); 3rd stage: transpression (R' = 1.81), NE‒SW compression (SHmax = N053°‒233° E); 4th stage: extension (R' = 0.31), E‒W extension (Shmin = N074°‒254° E). The 1st stage can be related to the oblique convergence between E and W Gondwanalands. The 2nd stage was probably concurrent with the N- to NNW-ward migration of the Central Eastern Desert syn-collisional delamination (triggering orogen-parallel extensional collapse) to the North Eastern Desert post-collisional delamination. The 3rd stage was controlled by the generally N‒S shortening which affected the northern and central ENS, post-dating the formation of volcanosedimentary Hammamat Basins. The 4th stage is akin to retreat of the Cadomian arc and the Red Sea rifting.

DEVELOPMENT AND VALIDATION OF SCALE FOR ASSESSMENT OF SAMYAK SNEHA LAKSHANAS

Chikitsa aims for Dhatusamyata by means of Shamana (pacificatory techniques) or Shodhana (evacuator or eliminatory treatments). Shamana addresses vitiated Doshas at their source rather than expelling them from the body, whereas Shodhana is concerned with the expulsion of Dushita Dosha or Mala Nirharana. Adequate ad-ministration Purvakarma, Shodhana achieves success by performing the Dosha Upasthita Avastha. Purvakarma for Shodana includes Deepana (digestive), Pachana (Ama detoxification), Rookshana, Snehana (oleation), and Swedana (sudation). In this context, Upasthita Dosha refers to the Dosha's presence in the Koshta for expulsion. Dosha is properly mobilized from Shakha to Koshta as a result of the actions of Snehana and Swedana. Pravicharana and Accha Snehapana are two methods, with Accha Snehapana being administered before Shodana. The purpose of this study is to develop and validate Samyak Sneha Jeerna Lakshana. Samyak Sneha Jeerna Lakshanas were gathered from classical texts during the development phase. Open-ended questionnaires were created for various domains and subdomains. The domains and questions were validated with 25 Snehapa-na for Virechana and Vamana patients. Based on the responses, a closed-ended questionnaire with a 5-to-7 Lik-ert scale was created. The reliability was assessed using Cronbach's Alpha(CA) test. The questions which didn’t give the acceptable CA were redacted and the questions with good and acceptable CA were retained. The final questionnaire was obtained with 41 questions after obtaining a CA of 0.836 in the 5th stage. The questionnaire obtained at the end of this research can be a self-administrable scale to analyze the Samyak Sneha Lakshanas in an individual who are undergoing shodhana both Vamana and Virechana. In this study ob-jective criteria are not developed to confirm Samyak Sneha Jeerna Lakshana. The final CA obtained i.e 7 to 1 was ‘good’ according to the rule of thumb for statistical reliability.

Wood decomposition, carbon, nitrogen, and pH values in logs of 8 tree species 14 and 15 years after a catastrophic windthrow in a mesic broad-leaved forest in the East European plain

There are limited studies of decomposition of coarse woody debris (CWD) in natural conditions of multi-species mesic broad-leaved forests. We investigated 49 lying logs of 7 angiosperm and 1 gymnosperm species 14 and 15 years after a mass windthrow in an old-growth broad-leaved forest. We observed high heterogeneity of logs in terms of decomposition stages: up to 4 stages in one transverse disk and up to 5 stages in longitudinal fragments of one log. Due to the high heterogeneity of logs, we investigated traits of wood samples rather than individual logs. Bulk density, carbon and nitrogen concentrations, and pH values were examined for 188 CWD samples of 8 species at 5 decomposition stages (from stage 1 least to stage 5 most decayed wood) and 24 control samples of live trees (3 replicates for each species; zero stage of decay). Changes of deadwood volume, carbon and nitrogen stocks over 14 and 15 years after the windthrow were estimated by re-surveying transects established in 2010. From the first to the fifth decay stage, the mean CWD density decreased for all species and averaged 54 ± 16 kg m−3 (SD) at the 5th stage of decay. Regarding decomposition rates, the tree species were arranged in descending rate from diffuse-porous (Populus tremula, Betula pendula, Tilia cordata, and Acer platanoides) to ring-porous (Ulmus glabra, Fraxinus excelsior, and Quercus robur) angiosperms and the coniferous Picea abies was after the diffuse-porous species series and before the ring-porous species. Carbon concentration ranged from 39 to 54 %, but did not change significantly during decomposition (mean value for all species in all decay stages was 45.8 ± 2.5 %). Nitrogen concentration (percentage of dry mass) increased with increasing decay stage and the mean value was 0.2 ± 0.1 % at the zero stage and 0.9 ± 0.5 % at the last decay stage. Despite the increase in N concentration, the mass N per volume decreased during decomposition for all samples considered together due to a significant decrease in wood density. The change in C/N values during deadwood decay reflected the transition of organic matter from the CWD to the SOM (soil organic matter) in forest ecosystems: the ratio C/N varied from 829 (Picea, zero stage) to 16 (Ulmus, last stage) with the mean values 286 ± 149 and 75 ± 45 at the zero and the last stages, respectively. We observed a quadratic trend in the variation of wood pH values by stages: pH mainly decreased from the initial values to the minimum in the middle stages of decay, and then increased in the advanced stages. The mean deadwood volume in the surveyed plots decreased 1.4-fold in the 14–15 years after the windthrow, but the mean stocks of carbon and nitrogen decreased 3 and 2-fold, respectively. Over time, the different decomposition rate of fallen logs leads to a decrease in the proportion of C and N stored in diffuse-porous species at the expense of an increase in this proportion in ring-porous species. This result is worth considering in a carbon management strategy: planting, maintaining stands, and retaining deadwood of ring-porous species, such as Quercus, Ulmus and Fraxinus, promote relatively long-term carbon storage.

A Method for Constructing Health Indicators of the Engine Bleed Air System Using Multi-Level Feature Extraction

Traditional methods are unable to effectively assess the health status of engine bleed air systems. To address the limitation, this paper proposes a methodology for constructing health indicators using multi-level feature extraction. First, this approach involves data-level feature extraction from Quick Access Recorder (QAR) data and employs a method of significance compensation to process the QAR data. Second, through unsupervised learning, the ResNet Deep Autoencoder (RDAE) is utilized to do the feature-level feature extraction from the processed data. This can solve the problem of lacking annotated data and obtain the health indicators of the engine bleed air system. Third, the method was experimented on one year of QAR data from a specific airline company. The results demonstrate that the RDAE approach achieves the best performance in constructing health indicators for the system. It achieves a miss rate of 0.0523 for the duct pressure of 5th stage bleed, reducing the miss rate by 0.2810 compared to Kernel Principal Component Analysis (KPCA). It also achieves a miss rate of 0 for the pre-cooler outlet temperature, reducing the miss rate by 0.0035 compared to the Deep Autoencoder (DAE). The results indicate that the proposed method provides a more effective assessment of the health status of the engine bleed air system.

Searching for Possible Design Solutions to Extend the Service Life of the HPC of a Powerful NPP Turbine after Damage to the Rotor Blades

The aim of this research was to analyze and evaluate possible design solutions for the flow path of the high-pressure cylinder (HPC) of the K-1000-60/3000 turbine and the possibility of their short-term implementation in a nuclear power plant after an accidental destruction of the working blades of the last stage of the HPC in conditions of the shortage of generating capacities and a tense operation state of the power system. To achieve the set goals, an appropriate methodology was developed; a mathematical model of thermo-and gas dynamic processes in the flow path of a steam turbine was improved. To make appropriate decisions on changing the design of the turbine, the necessary computational studies of the HPC of the K-1000-60/3000 turbine of the designed structure, the structure without rotor blades of the 5th stage and the structure with four first stages (without the fifth stage) were carried out for both turbine steam flows. The results of the study of thermo- and gas dynamic parameters of the flow showed the presence of differences in the operating conditions of all turbine stages, and these are the most significant for the 4th and 5th stages. A significant redistribution of thermal differences in the stages, and a decrease in the internal power of the HPC of the turbine by 35.5 MW and 6.6 MW respectively, as well as a decrease in their internal efficiency by 11.8 % and 2.1 %, respectively, were noted. Taking into account all the aspects related to the operation of the power unit, the most successful solution for the rapid resumption of operation of a powerful turbine plant used by a nuclear power plant after an accidental damage to the working blades is a HPC design without the working blades of the fifth stage. It is planned to carry out additional studies of the HPC design by replacing the nozzle apparatus of the fifth stage with an appropriate simulator.

PROCESS CAPABILITY ANALYSIS OF KATSUOBUSHI PRODUCTION AT PT. ABC

Process capability in processing fishery products is a future challenge for the fishing industry, one of which is katsuobushi. The capability process refers to how a company can produce according to specifications to make it effective and efficient. The absence of process capability in the company is the basis for stating an effective and efficient process. The proposed study is to determine the company's current condition and provide suggestions for process improvement and evaluation. Through assumptions, this study influences the results of Cp and Cpk, which are calculated by Minitab software version 21st as a method for measuring performance by identifying broad process tolerance production. In this study, the results for each data generated met data accuracy and standards. Still, at smoking 3rd and 4th stages, it was necessary to review specification values because of the value of Cpk &lt; 1.00. As a result, after improving the standard by the six sigma concept, Cpk values are obtained &gt; 1.00.

PHYSIOLOGICAL TOLERANCE TEST OF COMBINATION TREATMENT OF ANTIGEN-G AND CURCUMIN EXTRACT ON VEGF LEVELS, MORTALITY RATE OF BALB/c

The paper aimed to present the Physiological Tolerance Test of Combination Treatment of Antigen-G and Curcumin Extract on VEGF Levels, Mortality Rate of BALB/c. This research consists of several stages. The TMd-G antigen (ITMd) was isolated from the thoracic segments of Musca domestica larvae 4th instar stage and the thoracic segments of the adult cycle stage, 5 days old. The larvae were obtained from the rearing process using defined media. Curcumine extract (EK) was obtained from Curcuma xanthorrhiza rhizomes through the process of broyage, mixing, precipitation, lyophilization and collection of dry EK. The VEGF serum molecules were significant higher (P&lt;0.05) in Ek20Ag3 to Ek20Ag9 then in other combination treatment. The zero-mortality rate obtained in using AgLMd.

Mapping and validation of a novel major QTL for resistance to stripe rust in four wheat populations derived from landrace Qishanmai.

Wheat yield has been constrained by stripe rust disease globally. A wheat landrace (Qishanmai, QSM) consistently showed lower stripe rust severities in multiple year studies than susceptible check varieties including Suwon11 (SW) at the adult plant stage. To detect QTL for reducing the severity in QSM, 1218 recombinant inbred lines (RILs) were developed from SW × QSM. QTL detection was conducted firstly using 112 RILs selected for similarity in pheno-morphological characters. The 112 RILs were assessed for stripe rust severity at the 2nd leaf, 6th leaf and flag leaf stages under field and greenhouse conditions, and genotyping was done primarily with a single nucleotide polymorphism (SNP) array. On the basis of these phenotypic and genotypic data, a major QTL (QYr.cau-1DL) was detected on chromosome 1D at the 6th leaf and flag leaf stages. Further mapping was conducted by genotyping 1218 RILs using new simple sequence repeat (SSR) markers, which were developed by referring to the sequences of the wheat line Chinese Spring (IWGSC RefSeq v1.0). QYr.cau-1DL was mapped within a 0.5 cM (5.2 Mb) interval delimited by the SSR markers 1D-320.58 and 1D-325.79. These markers were applied to select for QYr.cau-1DL by screening F2 or BC4F2 plants of the wheat crosses RL6058 × QSM, Lantian10 × QSM and Yannong21 × QSM. F2:3 or BC4F2:3 families derived from the selected plants were assessed for stripe rust resistance in the fields of two locations and in a greenhouse. Wheat plants carrying the resistant marker haplotype in homozygous state for QYr.cau-1DL showed lower stripe rust severities (by 44% to 48%) than plants lacking this QTL. The trial of RL6058 (a carrier of Yr18) × QSM also indicated that QYr.cau-1DL had larger effect than Yr18 on reducing severity; they acted synergistically, yielding an elevated level of stripe rust resistance.

Determination of Nutritional Value and Methane Production Potential of Phacelia tanacetifolia in Different Stages of Growth

Backround: This study was carried out to determine the nutritional value and methane production potential of Phacelia tanacetifolia plant in different growth stages in April-May year in the ecological conditions of the province of Adiyaman in Turkey. Methods: Phacelia (Phacelia tanacetifolia) plant samples were collected with four replications in four different stages, pre-flowering, early flowering, mid-flowering and end of flowering (seeding). The nutrients of the feed samples were determined. The gas production was measured after 24 hours of incubation of the forage with rumen fluid. Result: The effect of harvest time on chemical composition was highly significant. In vitro gas production was lowest in stage I. and highest in II. The methane (CH4) lowest production values were found in the 1st stage, whereas the highest value was observed in the 4th stage (seed-binding stage), where the difference was statistically significant. ME was 7.49, 9.54, 8.90 and 8.61 MJ/kg DM respectively and the difference between the stages was statistically significant. It was concluded that Phacelia tanacetifolia, which is known as an important nectar source in the world and in Turkey. It is appropriate to harvest the phacelia plants in the second stage, i.e., at early flowering for higher ME contents and OMD.

LIFE TABLE ATTRIBUTES AND FEEDING EFFICIENCY OF OENOPIA SAUZETI (MULSANT) (COLEOPTERA: COCCINELLIDAE) FED ON APHID (APHIS GOSSYPII)

Ladybird beetles are play vital role in agroecosystem due to successful and efficient feeding behavior against aphids, the present research experiment was carried to find out the life history and feeding efficacy of oenopiasauzetifed on Aphis gossypii,at the laboratory of Biological Control, integrated pest management programme (IPMP), National Agriculture Research Centre (NARC), Islamabad. The results of the experiment revealed that the mean developmental time of immature stage 3rd and 4th instar have a significant difference with 3.72±0.11 and 3.87±0.036 days, while the egg to adult emergence was 39±0.35 days. The longevity has significant difference in male and female with the higher values at female 34.0 days. Similarly, the feeding efficiency was maximum in O. sauzeti females which feeds on 2227.1 mean number of Aphis gossypii, while the (409.25±1.42) mean numbers of aphids were consumed by larva. The maximum aphid consumption was observed at 4th instar larva (198.98±0.51 aphid) followed by 3rd instar larva (128.57±0.59 aphid). The mean oviposition time of O. sauzetion A. gossypii was recorded as 21.36 days and average eggs laid female-1 day-1 was 20.53, while the total laid eggs given by a female were 253.60. The apparent mortality (100qx) was maximum at egg stage (24) and the mortality survival ratio (MSR) was highest at pupal stage (0.53), similarly, the indispensable mortality (IM) was also observed high at pupal stage. The maximum survival fraction (Sx) was observed at the 4th larval stage (0.91) and the maximum life expectancy (ex) was observed at 1st larval instar (4.51) days. The total killer power (k-value) of the O. sauzetifor the complete generation was 0.49. This will help to develop an ecofriendly managing approach for the minimizing the aphids infestationin economical important crops.

A competing risk-based nomogram to predict cancer-specific survival in patients with retroperitoneal leiomyosarcoma

Considering the rarity and aggressive nature of retroperitoneal leiomyosarcoma (RLMS), several prognostic factors might contribute to the cancer-specific mortality of these patients. This study aimed to construct a competing risk-based nomogram to predict cancer-specific survival (CSS) for patients with RLMS. In total, 788 cases from the Surveillance, Epidemiology, and End Results (SEER) database (2000–2015) were included. Based on the Fine & Gray's method, independent predictors were screened to develop a nomogram for predicting 1-, 3-, and 5-year CSS. After multivariate analysis, CSS was found significantly associated with tumor characteristics (tumor grade, size, range), as well as surgery status. The nomogram showed solid prediction power and was well calibrated. Through decision curve analysis (DCA), a favorable clinical utility of the nomogram was demonstrated. Additionally, a risk stratification system was developed and distinctive survival between risk groups was observed. In summary, this nomogram showed a better performance than the AJCC 8th staging system and can assist in the clinical management of RLMS.

MORPHOLOGICAL CHANGES OF RATS’ CEREBRAL CORTEX UNDER CONDITIONS OF DMH-INDUCED EXPERIMENTAL CARCINOGENESIS

Abstract. The progression of the endogenous intoxication syndrome accompanies the development of any neoplasm. The infl uence of toxic products leads to signifi cant disruptions in homeostasis, which seriously aff ects the central nervous system, specifi cally the cortex of the large hemispheres. Therefore, the purpose of our study was to establish changes in the morphological reorganization of the cortex of the large hemispheres of rats with DMG-induced adenocarcinoma in situ. The study used 85 mature white male rats. All animals were divided into 2 groups: I – 35 intact animals and II – 50 animals with DMG-induced colon adenocarcinoma.Histological slides of the brain were prepared by the generally accepted methodology. The drugs were studied using a MICROmed SEO SCAN light microscope and photo- documented using a Vision CCD Camera. The histological organization of the cerebral cortex of intact animals had typical histoarchitectonics. In the fi rst three stages of the experiment, moderate changes in the morphological organization of the cortex were detected compared to the control group. The fourth stage of DMG-induced carcinogenesis modeling was characterized by the manifestation of morphological changes, a decrease in the number of normochromic, an increase in hypo- and hyperchromic neurocytes, and the appearance of cell alteration signs. Typical for this period also was the progression of hemodynamic disorders: venous hyperemia, stasis, aggregation of erythrocytes («sludge»), and an increase in perivascular and pericellular edema. At the 5th and 6th stages of the experiment, a violation of the cytoarchitectonics of the cortex was revealed. Destructive changes in neurocytes increased. Betz cells were in a state of tigrolysis, sometimes in total one. A decrease in the Nissl substance in neurons confi rmed their impaired functional capacity. Increased permeability of capillary walls led to severe pericellular edema of neurocytes. Intracellular edema was also detected in most neurocytes. The 7th stage of the induced carcinogenesis was characterized by the presence of severe destructive changes in neurons: an increase in the number of hypo-, hyperchromic, sharply hypo-, and sharply hyperchromic neurocytes, a fragmentation of neurocyte neuroplasm, most of Betz cells in a state of total tigrolysis, a decrease in the content of Nissl substance in them, up to its complete absence. Blood capillaries had a deformed wall and signifi cantly enlarged perivascular spaces. Consequently, the study established the development and dynamics of alterative changes in the neurocytes of the cerebral cortex caused by DMG-induced carcinogenesis. These disorders were characterized by alteration of neurocytes and progressive microcirculation disorders. All identifi ed morphological changes in the brains of aff ected animals prove their dependence on the duration of carcinogen exposure.&#x0D;

Pengembangan Media Game Based Learning (GBL) Pada Pembelajaran Dasar Kuliner Siswa SMK Program Keahlian Kuliner Kelas X

This study aims to determine: (1) the result of development of media GBL used in basic culinary learning class X, (2) feasibility of GBL media for basic culinary learning for class X culinary vocational students, (3) Vocational students’ responses to GBL media in basic culinary learning. This research method is R&amp;D with Plomp development model with five stages (prelimenary investigation, design, realization, test;evaluation;and revision, implemented), but due to research limitations, this research was carried out until 4th stage. The media validation involved three material experts and three media experts. The research object is a wordwall game and students responses. The instruments used for the feasibility of the game include material validation, media, and questionnaires to see student responses. As for the technique of data analysis carried out quantitatively. The result showed: (1)the result of the media that had been made in from of a QR code that can be scanned by readers, (2) the result of material and media validation with an average value 77,86% and 88,94% included worth with a little revision and (3) student responses have an average score 89,05% with very good interpretation. This indicates that the development of wordwall game can be used in learning.

Development and validation of a novel nomogram to predict the overall survival of patients with large cell lung cancer: A surveillance, epidemiology, and end results population-based study

BackgroundLarge cell lung cancer (LCLC) is a rare subtype of non-small cell lung carcinoma (NSCLC), and little is known about its clinical and biological characteristics. MethodsLCLC patient data were extracted from the Surveillance, Epidemiology, and End Results (SEER) database between 2004 and 2015. All patients were randomly divided into a training group and a validation group at a ratio of 7:3. The independent prognostic factors that were identified (P < 0.01) by stepwise multivariate Cox analysis were incorporated into an overall survival (OS) prediction nomogram, and risk-stratification systems, C-index, time-ROC, calibration curve, and decision curve analysis (DCA) were applied to evaluate the quality of the model. ResultsNine factors were incorporated into the nomogram: age, sex, race, marital status, 6th AJCC stage, chemotherapy, radiation, surgery and tumor size. The C-index of the predicting OS model in the training dataset and in the test dataset was 0.757 ± 0.006 and 0.764 ± 0.009, respectively. The time-AUCs exceeded 0.8. The DCA curve showed that the nomogram has better clinical value than the TNM staging system. ConclusionsOur study summarized the clinical characteristics and survival probability of LCLC patients, and a visual nomogram was developed to predict the 1-year, 3-year and 5-year OS of LCLC patients. This provides more accurate OS assessments for LCLC patients and helps clinicians make personal management decisions.

Relative Productivity of Mosquito Larval Habitats for Abundance and Immature stages Distribution in Relation to Physicochemical Properties in Niger State, Nigeria

Immature mosquitoes were evaluated for the productivity of the breeding habitats using field cages in Niger State. Mosquitoes were sampled weekly both outside and inside field experimental cages by dipping and emptying the cages in a bowl container and identified using microscope-guided standard morphological keys. Three genera namely, Aedes, Anopheles, and Culex, were encountered and the mean immature abundance rate (IAR), in gutters, follows decreasing order and dominated by Culex (93.16±10.79%) &gt; Anopheles (17.79±2.23%) &gt; Aedes (15.77±2.12 %). In swamps, Anopheles highest mean (78.04±8.00%), followed by Culex (28.13±2.55%) and Aedes(14.54±1.56%). Similarly, in large water bodies, the distribution decreases as; Anopheles (74.33±7.07%)&gt; Culex (27.14±2.50%)&gt;Aedes (12.91±1.30%). In terms of immature productivity rate (IPR) among the three genera, Culex had the highest mean in gutters (191.29±22.37%) followed by Anopheles in large waters and swamps (148.03±14.81% and 153.15±15.92%) while lowest was Aedes (25.57±2.42%) in large water. Furthermore, for the density of immature, 1st stage (L1) recorded the highest followed by 2nd stage (L2), followed by 3rd stage (L3), and the least was the 4th stage (L4) for IAR and IPR. For the estimation of breeding habitats, the cage sampling method is the most reliable (709.86±73.63 mosquitoes) when compared with dipping techniques that recorded 324.06±44.00 mosquitoes. Mosquito physicochemical conditions varied significantly with DO (8.83±0.40) in large water bodies; nitrate (4.57±0.55), phosphate (3.26±0.73), calcium (88.19±12.79), sulphur (28.23±2.45), potassium (10.63±0.76), chlorine (87.52±20.89) and alkalinity (187.00±26.39) in the gutters. However, a positive correlation between rainfall and mosquito species (r = 0.523 to 0.801) as well as between relative humidity and mosquitoes (r= 0.236 to 0.551, p&gt;0.05) on mosquito populations. Only Anopheles in large water habitats was significant (0.801*, p&gt;0.05). The findings revealed disparities in the abundance and larval habitat production rates of the mosquito genera and associated positively with some physicochemical and metrological cues. Consequently, an effective tactic must be developed for mosquito control, particularly in the most highly recognized breeding habitats.