ABSTRACT In this paper, the laser ultrasonic non-destructive testing technology was used to detect the microstructure and mechanical properties of selective laser melting (SLM) 316 L stainless steel after post-treatment. The microstructure and mechanical properties of additively manufactured 316 L stainless steel were measured by Scanning electron microscopy (SEM), Electron backscatter diffraction (EBSD), tensile testing, etc. The laser ultrasonic longitudinal wave signal characteristic values were coupled with the microstructure and mechanical properties of SLM 316 L stainless steel. The results show that: The yield strength has a good correlation with wave speed and attenuation. With the increase of holding temperature and holding time, the grain size increases, the low angle grain boundary decreases, the yield strength gradually decreases from 409 MPa to 359 MPa, the wave velocity increases from 5579 m/s to 5700 m/s, and the frequency domain attenuation coefficient increases from 0.13 dB/mm to 0.16 dB/mm, the central frequency domain is about 10 MHz. The ultimate tensile strength is related to the frequency domain attenuation. The ultimate tensile strength decreases with the increase of the frequency domain attenuation coefficient. It can provide a reference for the evaluation of mechanical properties by laser ultrasonic testing.