3rd Round Research Articles

Cortisol and Testosterone Responses in Chess Players during an International Chess Tournament

Chess is a strategic atypical sport and a highly cognitive task demanding mental and physical alertness, in which players are deeply committed and experience intense emotions. Evidence supports that chess elicits physiological responses and induces stress-associated hormonal changes. PURPOSE: This study investigated potential changes in free salivary cortisol and testosterone in chess players during a tournament. METHODS: Thirty male active chess players participated in the study (age: 43.2±12.4 yrs, Elo score: 1903.2±341.5), competing in a 9-round chess tournament (Swiss pairing system). Unstimulated mixed saliva samples were collected in salivate swabs at 3 time points: baseline, after the 2nd and 3rd round and, for the 10 finalists also after the 6th and 7th round (19:00-23:25 pm.).The saliva samples were analyzed for cortisol and testosterone by ELISA and student T-test was used for statistics. RESULTS: Testosterone levels exhibited a trend of decrease, from 77.7±105.1 ng/ml at baseline to 38.7±46.6 ng/ml after the 2nd and 3rd round, to 25.3±20.7 ng/ml after the 6th and 7th round, however without reaching statistical significance (p>0.05). Cortisol levels increased from 4.4±3.3 ng/ml at baseline to 7.6±6.8 ng/ml after the 2nd and 3rd round in all participants (p<0.05). In the 10 finalists, however, cortisol showed no significant changes through the 3 time points (from 3.5±2.8 ng/ml to 6.0±4.8 ng/ml to 5.3±2.9 ng/ml; p>0.05). Significant percentage changes of cortisol were found compared to baseline (277±384.5% ng/ml and 207.6±113.7% ng/ml; p<0.01). Interestingly, in the finalists, cortisol to testosterone ratio exhibited a significant increase only after the 2nd and 3rd round, from 0.1±0.1 to 0.4±0.3 (p<0.01), contrary to the all participants’ ratio, which declined from 1.4±6.8 to 0.4±0.3 (p>0.05). CONCLUSIONS: Our findings indicate that testosterone levels are not significantly affected by the stress of the game in contrast to cortisol levels, which are significantly increased. Interestingly, although cortisol levels of top chess players (finalists) did not show significant changes during the tournament, however their cortisol to testosterone ratio was elevated after the first rounds, implying an increased physiological stress of these players at the beginning of the tournament.

SUN-LB068 Ectopic Cushing Syndrome Due to a Metastatic Neuroendocrine Tumor Treated with Metyrapone

Introduction: We describe a patient with a non-secretory low grade neuroendocrine tumor that transformed into a high grade carcinoma causing Cushing syndrome that was medically managed with metyrapone. Clinical Case: A 53 year old woman with a history of widely metastatic neuroendocrine tumor for 5 years was treated with somatostatin analogues, multiple chemotherapeutic agents, radiation therapy, and recently peptide receptor radionuclide therapy (PRRT). Some of her tumors were responding to PRRT, except that 1 month after her 3rd round of PRRT, she presented with profound muscle weakness, confusion, hypokalemia (K = 3.1 meq/L), and new onset of diabetes mellitus (glucose 319 mg/dL). Ectopic Cushing syndrome was diagnosed by ACTH of 748 pg/mL (n 7.2 - 63 pg/mL), a serum cortisol of 127 mcg/dL (n 6.7 - 22.6 mcg/dL), and a 24 hour urine free cortisol of 20,328 mcg/L (n 0 - 50 ug/24 hr). Her labs also showed pancytopenia including platelet count of 47 E3/ul (n 150 - 450 x10E3/uL). Imaging revealed growing necrotic liver metastases and thickened adrenal glands. Biopsy of the liver lesions revealed a high grade metastatic small cell neuroendocrine carcinoma with a mitotic rate of 49/10 HPF and Ki-67 of 70%. Interestingly, the neuroendocrine tumor cells in the liver biopsy did not stain for ACTH. This diverged greatly from pathology on initial diagnosis which revealed a low-grade well differentiated neuroendocrine tumor with a mitotic rate 2/10 HPF and Ki-67of 4-5%. It was clear from the patient’s clinical presentation and pathology results that her tumor had evolved into a poorly differentiated tumor secreting either ACTH and/or CRH resulting in Cushing syndrome. She was deemed a poor surgical candidate for adrenalectomy due to critical illness and advanced cancer. Elevated transaminases related to necrotic liver lesions precluded use of ketoconazole. Thus, medical therapy was initiated using metyrapone as well as spironolactone, potassium supplements, and insulin. Serum cortisol improved rapidly to 20 mcg/dl. 24 hour urine free cortisol improved to 1,129 mcg/ 24 hours with metyrapone 250mg twice daily and to 429 mcg/ 24 hours with metyrapone 250 mg every 6 hours. Her thrombocytopenia worsened with a nadir of 5 E3/ul but recovered to 127 E3/ul with continuation of metyrapone. Bone marrow biopsy performed at that time found low grade neuroendocrine tumor cells that did not stain for ACTH. Conclusion: The transformation of an indolent low grade neuroendocrine tumor to a high grade neuroendocrine carcinoma was accompanied by an ectopic Cushing syndrome. The cortisol excess was controlled rapidly and effectively with metyrapone. Thrombocytopenia was temporally related to the severe hypercortisolism and platelets improved to near normal with correction of cortisol. Unless otherwise noted, all abstracts presented at ENDO are embargoed until the date and time of presentation. For oral presentations, the abstracts are embargoed until the session begins. s presented at a news conference are embargoed until the date and time of the news conference. The Endocrine Society reserves the right to lift the embargo on specific abstracts that are selected for promotion prior to or during ENDO.

Long MMA fights technical-tactical analysis of mixed martial arts: implications for assessment and training

ABSTRACTThis study aimed to compare time-motion and technical-tactical behaviours of professional mixed martial arts (MMA) bouts with a focus on longer bouts that were finished during 3rd round vs. 10 4th round vs. 5th round, comparing rounds. We analysed 779 rounds (264 bouts – 3rd round n = 120; 4th round n = 92; ©and; 5th round n = 52) which were compared by rounds. Our main results showed that bouts with 5th showed higher percentage/round of lowintensity time than the 3rd round (79% vs. 75%) and the 4th rounds demonstrated higher percentage/round of low-intensity time effort 20 than other rounds (84% vs. 76%). Regarding striking actions, 1st round (39.5 ± 17.6) and 4th round (19.8 ± 12.5) showed lower frequency of strikes attempted than 2nd round (46.1 ± 21.9) and 3rd round (46.1 ± 27.0). This study demonstrated new parameters for long MMA bouts with 3, 4 and 5 ending-rounds and other rounds.

Tragic Aftermath of Californian Jungle Fire and Hawaiian Volcano Outburst, a Warning about Persistently Rising Global Warming, Resulting into High Rated Disaster’s Chain

Earthglobe is almost at flash point with respect to global heat contents and temperature and any sizeable further addition of heat to its environment will result into huge disaster of life, property and infrastructure at multiple locations on the globe. The recent example of this is the aftermath of Cali-fornian forest fires (2017-2018) and Hawaii volcano eruption as elaborated in this treatise. This has resulted into 7 main catastrophic events over the Earth globe in its first round, while in 2nd round, all the previous same 7 or similar event in the same or an area close to it are repeated with one more event added in the list. These wildfires add large quantity of heat directly to the globe and even more through abstraction of out going global heat radiation by their generated green house gasses, pollution and soot. The 2nd round is perhaps also supported by Hawaii volcanic eruption. The 3rd round has mainly completed and few expected events are yet in pipe line, but California jungle fire of November 2018 has strengthened its attacks again. The mechanics and tracks of its attacks with pulled in of its allies has been elaborated in this work with role of this wildfire and its all children; heat, soot, pollution and green house gasses in possible boast of global heat contents and temperature. It is observed that disastrous aftermath from all such heating events in the north are contained mainly in Northern Hemisphere, while contrary to this; some of such events in the south have hard strikes on the North. It is requested that Global heat contents rise, jungle fire and volcanic eruptions must be completely stopped by all means because this causes widespread disaster mainly over the tropical regions of Globe as experienced from Californian Jungle Fire.

Prevalence of Use of Tobacco Products among Aizawl Population

Diseases arising due to usage of tobacco is one of the most common cause of premature death & disability around the world. In 3rd round of Nationwide survey across India held in 2005–06 by National Family Health Survey estimated that tobacco consumption habit was among 57.6% males & 10.8% females. Mizoram is a small hilly state located in Northeast of India.

Contribution and Influence of the Civil Society for the New Health Warning Label of Tobacco Products in Brazil

Background and context: In April 2017, National Agency for Health Surveillance (Anvisa) launched a public consultation for the revision of Resolution Number 335/2003, on health warning labels. Aim: A group of representatives from civil society and tobacco control advocates in Brazil met in person and virtually to make contributions to the Anvisa´s proposal for new health warning label for tobacco products packages, considering the civil rights for participation and collaboration to Brazilian policies provided by public consultation. Strategy/Tactics: The advocates and researches consulted the Framework Convention on Tobacco Control of the World Health Organization and its Guideline for Article 11 (Packaging and Labeling of Tobacco Products) implementation adopted in 2008 based on scientific studies and successful experiences in other countries. In addition, international researchers and representatives of international institutions were also consulted to collect successful examples of health warnings in the world. Program/Policy process: In October 2017, the Anvisa Board of Directors at a public ordinary meeting approved the new health warnings and in December of the same year, published the new resolution about it. Outcomes: In a comparative analysis, even if all the recommendations made in the CP have not been complied with, the influence of the contributions of the group of tobacco control advocates to the final version of the warnings is evident. The main contributions considered by Anvisa were: 1) to increase the effectiveness of the health warnings using colored images instead of black and white images, 2) the use of yellow in the upper and lower tiers was also one of the points suggested by the group, and 3) the concept proposed by the group for side warnings was fully complied. What was learned: Civil society participation for the agency´s public policy implementation process is essential and has been possible through the regulatory agenda, public consultations and regular public meetings. However, as is expected, the regulated sector has been quite active. The group recognizes the importance of the Anvisa measure in proposing new health warnings, however, significant recommendations have not been adopted and it may interfere with the goals of the measure. Anvisa´s strategy of allowing the social participation is essential, in accordance with Universal Health System principles and must be maintained. Regarding to the next health warnings and messages on packages of tobacco products, it would be greater to consider higher frequency of updating the images and recover the scientific process of development of the 3rd round of health warnings labels coordinated by the National Institute of Cancer in 2007/2008.

Novel Method for Structure-Activity Relationship of Aptamer Sequences for Human Prostate Cancer.

Prostate cancer (PCa) is one of the most common malignancies in men and seriously threatens men’s health. Developing aptamer probes for PCa cells is of great significance for early diagnosis and treatment of PCa. This paper reports a classification model for SELEX-based aptamers, which were obtained with PCa cell line PCa-3M-1E8 (highly metastatic tumor cell) as target cells and PCa cell line PCa-3M-2B4 (low metastatic tumor cell) as control cells. On the basis of the SELEX principle, 100 oligonucleotide sequences from the 3rd round of SELEX were defined as low affinity and specificity aptamers, and 100 sequences from the 11th round were set as high affinity and specificity aptamers. Seven molecular descriptors were used for the classification model, which were calculated from amino acid sequences translated from DNA aptamer sequences with DNAMAN software. The classification model based on binary logical regression analysis has prediction accuracies, sensitivity, and specificity of about 80% for both the training set and test set. Therefore, it is feasible to calculate molecular descriptors from amino acid sequence translated from DNA aptamer sequences and develop a classification model for PCa cell line PCa-3M-1E8.

A methodological study to develop standard operational protocol for feeding preterm neonates with expressed breast milk

Objectives: The objectives of this study were to assess the current practices, to identify barriers and facilitators for feeding preterm neonates with expressed breast milk (EBM), and to develop standard operational protocol (SOP) and checklist for feeding preterm neonates with EBM. Materials and Methods: The methodological research design was used in the present study, and it was conducted in a tertiary level neonatal unit, North India. Sample population was nursing staff, preterm neonates, and their mothers, admitted in neonatal unit. To enroll nursing staff and preterm neonates, total enumeration sampling technique was used, whereas purposive sampling technique was used to select mothers of preterm neonates. Research project was carried out in five phases. Results: After assessment of the current practices, focus group discussions were conducted with nursing staff and mothers to know the barriers and facilitators. Findings of discussions were used to develop SOP and checklists. Three Delphi rounds were conducted to finalize the content and calculate content validity index (CVI). After the 3rd round, the CVI was 0.99 for SOP and 1.00 for checklists. Cronbach

Cube-like Attack on Round-Reduced Initialization of Ketje Sr

This paper studies the Keccak-based authenticated encryption (AE) scheme Ketje Sr against cube-like attacks. Ketje is one of the remaining 16 candidates of third round CAESAR competition, whose primary recommendation is Ketje Sr. Although the cube-like method has been successfully applied to Ketje’s sister ciphers, including Keccak-MAC and Keyak – another Keccak-based AE scheme, similar attacks are missing for Ketje. For Ketje Sr, the state (400-bit) is much smaller than Keccak-MAC and Keyak (1600-bit), thus the 128-bit key and cubes with the same dimension would occupy more lanes in Ketje Sr. Hence, the number of key bits independent of the cube sum is very small, which makes the divide-and-conquer method (it has been applied to 7-round attack on Keccak-MAC by Dinur et al.) can not be translated to Ketje Sr trivially. This property seems to be the barrier for the translation of the previous cube-like attacks to Ketje Sr. In this paper, we evaluate Ketje Sr against the divide-and-conquer method. Firstly, by applying the linear structure technique, we find some 32/64-dimension cubes of Ketje Sr that do not multiply with each other as well as some bits of the key in the first round. In addition, we introduce the new dynamic variable instead of the auxiliary variable (it was used in Dinur et al.’s divide-and-conquer attack to reduce the diffusion of the key) to reduce the diffusion of the key as well as the cube variables. Finally, we successfully launch a 6/7-round 1 key recovery attack on Ketje Sr v1 and v2 (v2 is presented for the 3rd round CAESAR competition.). In 7-round attack, the complexity of online phase for Ketje Sr v1 is 2 113 , while for Ketje Sr v2, it is 2 97 (the preprocessing complexity is the same). We claim 7-round reduced Ketje Sr v2 is weaker than v1 against our attacks. In addition, some results on other Ketje instances and Ketje Sr with smaller nonce are given. Those are the first results on Ketje and bridge the gaps of cryptanalysis between its sister ciphers – Keyak and the Keccak keyed modes.

Cube-like Attack on Round-Reduced Initialization of Ketje Sr

This paper studies the Keccak-based authenticated encryption (AE) scheme Ketje Sr against cube-like attacks. Ketje is one of the remaining 16 candidates of third round CAESAR competition, whose primary recommendation is Ketje Sr. Although the cube-like method has been successfully applied to Ketje’s sister ciphers, including Keccak-MAC and Keyak – another Keccak-based AE scheme, similar attacks are missing for Ketje. For Ketje Sr, the state (400-bit) is much smaller than Keccak-MAC and Keyak (1600-bit), thus the 128-bit key and cubes with the same dimension would occupy more lanes in Ketje Sr. Hence, the number of key bits independent of the cube sum is very small, which makes the divide-and-conquer method (it has been applied to 7-round attack on Keccak-MAC by Dinur et al.) can not be translated to Ketje Sr trivially. This property seems to be the barrier for the translation of the previous cube-like attacks to Ketje Sr. In this paper, we evaluate Ketje Sr against the divide-and-conquer method. Firstly, by applying the linear structure technique, we find some 32/64-dimension cubes of Ketje Sr that do not multiply with each other as well as some bits of the key in the first round. In addition, we introduce the new dynamic variable instead of the auxiliary variable (it was used in Dinur et al.’s divide-and-conquer attack to reduce the diffusion of the key) to reduce the diffusion of the key as well as the cube variables. Finally, we successfully launch a 6/7-round1 key recovery attack on Ketje Sr v1 and v2 (v2 is presented for the 3rd round CAESAR competition.). In 7-round attack, the complexity of online phase for Ketje Sr v1 is 2113, while for Ketje Sr v2, it is 297 (the preprocessing complexity is the same). We claim 7-round reduced Ketje Sr v2 is weaker than v1 against our attacks. In addition, some results on other Ketje instances and Ketje Sr with smaller nonce are given. Those are the first results on Ketje and bridge the gaps of cryptanalysis between its sister ciphers – Keyak and the Keccak keyed modes.

Conditional Cube Attack on Round-Reduced ASCON

This paper evaluates the secure level of authenticated encryption Ascon against cube-like method. Ascon submitted by Dobraunig et al. is one of 16 survivors of the 3rd round CAESAR competition. The cube-like method is first used by Dinur et al. to analyze Keccak keyed modes. At CT-RSA 2015, Dobraunig et al. applied this method to 5/6-round reduced Ascon, whose structure is similar to Keccak keyed modes. However, for Ascon the non-linear layer is more complex and state is much smaller, which make it hard for the attackers to select enough cube variables that do not multiply with each other after the first round. This seems to be the reason why the best previous key-recovery attack is on 6-round Ascon, while for Keccak keyed modes (Keccak-MAC and Keyak) the attacked round is no less than 7-round. In this paper, we generalize the conditional cube attack proposed by Huang et al., and find new cubes depending on some key bit conditions for 5/6-round reduced Ascon, and translate the previous theoretic 6-round attack with 2 66 time complexity to a practical one with 2 40 time complexity. Moreover, we propose the first 7-round key-recovery attack on Ascon. By introducing the cube-like key-subset technique, we divide the full key space into many subsets according to different key conditions. For each key subset, we launch the cube tester to determine if the key falls into it. Finally, we recover the full key space by testing all the key subsets. The total time complexity is about 2 103.9 . In addition, for a weak-key subset, whose size is 2 117 , the attack is more efficient and costs only 2 77 time complexity. Those attacks do not threaten the full round (12 rounds) Ascon.

Conditional Cube Attack on Round-Reduced ASCON

This paper evaluates the secure level of authenticated encryption Ascon against cube-like method. Ascon submitted by Dobraunig et al. is one of 16 survivors of the 3rd round CAESAR competition. The cube-like method is first used by Dinur et al. to analyze Keccak keyed modes. At CT-RSA 2015, Dobraunig et al. applied this method to 5/6-round reduced Ascon, whose structure is similar to Keccak keyed modes. However, for Ascon the non-linear layer is more complex and state is much smaller, which make it hard for the attackers to select enough cube variables that do not multiply with each other after the first round. This seems to be the reason why the best previous key-recovery attack is on 6-round Ascon, while for Keccak keyed modes (Keccak-MAC and Keyak) the attacked round is no less than 7-round. In this paper, we generalize the conditional cube attack proposed by Huang et al., and find new cubes depending on some key bit conditions for 5/6-round reduced Ascon, and translate the previous theoretic 6-round attack with 266 time complexity to a practical one with 240 time complexity. Moreover, we propose the first 7-round key-recovery attack on Ascon. By introducing the cube-like key-subset technique, we divide the full key space into many subsets according to different key conditions. For each key subset, we launch the cube tester to determine if the key falls into it. Finally, we recover the full key space by testing all the key subsets. The total time complexity is about 2103.9. In addition, for a weak-key subset, whose size is 2117, the attack is more efficient and costs only 277 time complexity. Those attacks do not threaten the full round (12 rounds) Ascon.

Comparative Analysis of Game Content of Male⋅Female Collage Taekwondo Players

This research analyzed each game record regarding scores per weight, gender, rotation, and forbidden behavior targeting 32 players through total 16 games of final per each 8th level of male and female collage students who participated in the preliminaries to select 2017 national team combined with the 46th association president award national group Taekwondo contest, and the research results are as follows. First, total score of the whole 16 games of male and female college students appeared 169, each score per game appeared 10.5. Second, difference in score according to male and female appeared, among total score 169, male displayed 90, female, 79, accordingly, male players displayed a big higher score than female players. Third, difference in score per rotation appeared in order of 3 rotations(38)>2 rotations(32)>1 rotation(20), female players also displayed 3 rotations(41)>2 rotations(20)>1 rotation(18), similar both in male and female. Fourth, the number of yellow card through the whole 16 games appeared total 41, average number of yellow card per game per weight appeared 2.5, and the number of yellow card per rotation appeared in order of 3 rotations(25)>2 rotations(12)>1 rotation(4). Fifth, among the total 41 yellow cards made through the whole 16 games in respect of gender, male players made 29 through 8 games, average 3.6 per game, while female players made 12 among 8 games, average 1.5 per game. Sixth, winners received 22 yellow cards among total 41, while the loser 19, accordingly, winners received more yellow cards. In case of male players, winners displayed 16, more than losers, 13. No deduction appeared both in male and female players through the games. As the research result, players received many yellow cards due to reinforcement of the judgment provision as the game rule changed, accordingly, a management plan for this is necessary. Various strategic training combined with lively and active offense technique and individual strength are required rather than passive game management for the players and the coaches, also, undesirable game operation such as delay of game and protection of score with ungentlemanly behavior as one is winning in the 3rd round should be rejected. Henceforth, for Korean students to hold priority in competition with global players, rapid adaptation to the revised game rule, more active game operation and strategies are required.

The mammalian skeletal muscle DHPR has larger Ca2+ conductance and is phylogenetically ancient to the early ray-finned fish sterlet (Acipenser ruthenus)

The L-type Ca2+ channel or dihydropyridine receptor (DHPR) in vertebrate skeletal muscle is responsible for sensing sarcolemmal depolarizations and transducing this signal to the sarcoplasmic Ca2+ release channel RyR1 via conformational coupling to initiate muscle contraction. During this excitation-contraction (EC) coupling process there is a slow Ca2+ current through the mammalian DHPR which is fully missing in euteleost fishes. In contrast to ancestral evolutionary stages where skeletal muscle EC coupling is still depended on Ca2+-induced Ca2+-release (CICR), it is possible that the DHPR Ca2+ conductivity during mammalian (conformational) EC coupling was retained as an evolutionary remnant (vestigiality). Here, we wanted to test the hypothesis that due to the lack of evolutionary pressure in post-CICR species skeletal muscle DHPR Ca2+ conductivity gradually reduced as evolution progressed. Interestingly, we identified that the DHPR of the early ray-finned fish sterlet (Acipenser ruthenus) is phylogenetically positioned above the mammalian rabbit DHPR which retained robust Ca2+ conductivity, but below the euteleost zebrafish DHPR which completely lost Ca2+ conductivity. Remarkably, our results revealed that sterlet DHPR still retained the Ca2+ conductivity but currents are significantly reduced compared to rabbit. This decrease is due to lower DHPR membrane expression similar to zebrafish, as well as due to reduced channel open probability (Po). In both these fish species the lower DHPR expression density is partially compensated by higher efficacy of DHPR-RyR1 coupling. The complete loss of Po in zebrafish and other euteleost species was presumably based on the teleost specific 3rd round of genome duplication (Ts3R). Ts3R headed into the appearance of two skeletal muscle DHPR isoforms which finally, together with the radiation of the euteleost clade, fully lost the Po.

Sexual History Taking Competency: A Survey among the Clinicians in Bangladesh

Introduction: Sexuality is a hidden topic and not talked much in countries like Bangladesh with strong cultural &amp; religious myths and having health seeking behavior of availing traditional healers and lack of specialized service center. Objective: It was aimed to see how often the clinicians ask about sexual issues in their clinical practice; the level of comfortability in inquiring about sex related problems and the opinion regarding sexual health services. Methods: A descriptive, observational, cross sectional analysis was carried out on willing clinicians who attended seminars on sexual dysfunction in Bangabandhu Sheikh Mujib Medical University. The data were collected by predesigned selfreporting questionnaire from clinicians attending in 3 different occasions at the same venue from 560 respondents as 125 in first round, 166 in second round and 269 in the third round. Nonprobability purposive consecutive sampling technique was chosen and data were analyzed by Statistical Package of Social Science (SPSS) 16.0 and Microsoft Excel 2007 version software. Results: At first round 53.6% were comfortable and 35% were uncomfortable asking sexual history with 26% facing discomfort asking, 12% uncomfortable with extreme age, and 12% with fear of offending the patients. In second round 42% clinicians were uncomfortable asking sexual history. In 3rd round survey, 37% of the respondents were uncomfortable to ask sexual history. Majority of the respondents (95%) agreed specialized set up should be established for patients with sexual dysfunction. Conclusion: Its first ever survey on clinicians of different discipline who are interested to deal with patients with sexual dysfunction with outcome recommendation of specialized set up for patients with sexual dysfunction.

The opinions of occupational physicians about maintaining healthy workers by means of medical examinations in Japan using the Delphi method.

In Japan, employee fitness for work is determined by annual medical examinations. It may be possible to reduce the variability in the results of work fitness determination, particularly for situation, if there is consensus among experts regarding consideration of limitation of work by means of a single parameter. Consensus building was attempted among 104 occupational physicians by employing a 3-round Delphi method. Among the medical examination parameters for which at least 50% of participants agreed in the 3rd round of the survey that the parameter would independently merit consideration for limitation of work, the values of the parameters proposed as criterion values that trigger consideration of limitation of work were sought. Parameters, along with their most frequently proposed criterion values, were defined in the study group meeting as parameters for which consensus was reached. Consensus was obtained for 8 parameters: systolic blood pressure 180 mmHg (86.6%), diastolic blood pressure 110 mmHg (85.9%), postprandial plasma glucose 300 mg/dl (76.9%), fasting plasma glucose 200 mg/dl (69.1%), Cre 2.0mg/dl (67.2%), HbA1c (JDS) 10% (62.3%), ALT 200 U/l (61.6%), and Hb 8 g/l (58.5%). To support physicians who give advice to employers about work-related measures based on the results of general medical examinations of employees, expert consensus information was obtained that can serve as background material for making judgements. It is expected that the use of this information will facilitate the ability to take appropriate measures after medical examination of employees.

Changes in blood lactate concentrations during taekwondo combat simulation.

The aim of this study was to analyze blood lactate response before, during and after simulated taekwondo fight in young male athletes. The experiment was composed of simulated official taekwondo fight. During the experiment a total of 7 blood samples (25 μL) were collected that following: at rest, after 1st, 2nd, and 3rd rounds, and 3, 5, and 10 min after the end of combat. The results showed that blood lactate concentrations [Lac]B increased significantly after 1st, 2nd, and 3rd rounds of the combat compared to the rested values. The [Lac]B after 3rd round was higher when compared to 3, 5, and 10 min of recovery. After 10 min of passive recovery, the [Lac]B was significantly lesser than 3 and 5 min of recovery, and 2nd round. These data showed that taekwondo requires high activation of anaerobic lactic metabolism, and improves the ability to rapidly remove the lactate from blood.

PTH-082 Factors associated with rating of care and the family and friends test: secondary analysis of the uk ibd audit inpatient experience survey

Introduction There is growing emphasis on subjective care ratings as a tool to drive standards. The simple ‘Friends and Family Test’ is offered to all NHS inpatients after discharge. We included a selection of ‘rating’ style questions in the UK IBD audit inpatient experience survey. 1 This secondary analysis further evaluated patient factors associated with variation in ratings and explored their correlation with survey items asking about hospital experiences relevant to UK IBD Standards. Method Responses to inpatient questionnaire (3 rd round UK IBD audit) were analysed. Factors associated with overall rating (‘Rating’: ‘How would you rate the care you received?’) and the Friends and Family Test (‘FFT’: ‘Would you recommend this hospital to your friends and family?’) were explored: age, gender, disease type, admission type (emergency medical; emergency surgery; elective surgery) and health state at the time of questionnaire completion (EQ5D). 15 items were mapped onto specific IBD standards. Results 2,067 respondents (UC 1,078, CD 989; 42.6% male; mean age 46.2 yrs; Elective surgery 20.7%, Emergency surgery 12.3%; 67.0% Medical therapy). Patients with CD had a lower QoL (EQ5D: UC 0.75 vs. CD 0.69, p Conclusion In IBD patients, ‘rating’ style questions are influenced by independent patient variables and current state of health and require careful interpretation. Correlation with questions relevant to IBD standards is variable, confirming that patient experience surveys should include items that map onto specific events and experiences rather than subjective ratings alone. Disclosure of interest None Declared. Reference UK IBD Audit. National report of the results of the UK IBD audit 3rd round inpatient experience questionnaire responses. https://www.rcplondon.ac.uk/projects/ibdauditround3

Ph.D.-7&lt;sup&gt;TM&lt;/sup&gt;肽库体内淘选卵巢癌A2780细胞特异性结合肽

卵巢癌是女性生殖器官常见的恶性肿瘤之一，卵巢上皮癌死亡率居各类妇科肿瘤的首位。探索高效安全的治疗手段，已经成为卵巢癌治疗领域的热点问题。目的：应用噬菌体展示技术体内筛选、鉴定并评价卵巢癌特异性结合七肽，为卵巢癌靶向给药系统的构建及肿瘤靶向诊疗技术的研究奠定基础。方法：噬菌体展示七肽库Ph.D.-7TM在卵巢癌A2780荷瘤模型体内进行3轮淘选。经ELISA、体内外结合、结合抑制和免疫荧光试验鉴定肿瘤阳性噬菌体单克隆及其对应展示肽的特异性，MTT试验、划痕试验考察靶向肽对相关细胞行为的影响。结果：测序结果中表达HGGVRLY肽(HGG肽)的噬菌体单克隆PC-H所占比例达到27%，ELISA中PC-H对A2780细胞显示出高亲和力及显著特异性(P < 0.05)。体内、体外反筛结果表明PC-H对A2780细胞和肿瘤组织具有很强亲和力。结合抑制实验及荧光显微成像证明了HGG肽对A2780细胞的靶向性，且HGG肽对肿瘤细胞的行为无显著影响，对正常细胞无抑制。结论：应用体内噬菌体肽库技术成功筛选出可与卵巢癌A2780细胞特异性结合的靶向肽HGGVRLY，其细胞相容性良好，可用于卵巢癌的诊断和靶向治疗。 In order to select the specific ligands for ovarian cancer, the in vivo phage display technology has been applied to screen the peptide targeting to ovarian cancer A2780 cells. Firstly, ovarian cancer A2780 cells were inoculated into the armpit of nude mice to establish a tumor-bearing animal model. Then in vivo biopanning was conducted for 3 rounds with the Ph.D.-7TM phage-displayed peptide library, and individual phage clones were picked for DNA sequencing after the 3rd round. Among the picked phage clones, the clone PC-H displaying heptapeptide HGGVRLY (peptide HGG) accounted for as high as 27 percent. The identification results by ELISA tests, in vivo and in vitro phage binding assays, competitive binding assays, and immunofluorescence assays indicated that both the phage clone PC-H and the peptide HGG could be preferentially bounded to A2780 cells and then concentrated in tumor tissues. Furthermore, the results of MTT assay and scratch assay revealed that peptide HGG possessed low cytotoxicity. Altogether, the peptide HGG screened with the Ph.D.-7TM exhibited the high affinity to A2780 cells and the targeting to the ovarian cancer tis-sues, and would have great potentials in the targeting drug delivery system for ovarian cancer.

Evaluation of metrological characteristics of spectrophotometric quantitative determination of paracetamol in tablets by specific absorbance

The validation characteristics of the spectrophotometric quantitative determination of paracetamol in tablets by specific absorbance according to the British Pharmacopoeia (BPh) have been evaluated. The results of paracetamol content of 83.59% and 84.39% in terms of the average mass of one tablet do not meet the permissible limits B ±5.0%. The peculiarities of the sample preparation method for quantitative determination of the active pharmaceutical ingredient in tablets has been discussed, and comparative analysis of “Dissolution” and “Assay” tests for paracetamol tablets according to the BPh has been conducted. We have suggested to make such changes at the stage of the sample preparation as “… place the flask in an ultrasonic bath for 30 min…” instead of “…shake for 15 minutes…”. The acceptance criteria of the assay method for paracetamol tablets have been calculated for permissible limits of ±5.0%, ±7.5%, ±10.0%. The results of the convergence and linearity research of the method meet requirements for the permissible limits of ±5.0%. The results of the intermediate precision re-search of the method meet requirements for the permissible limits of ±7.5%. The results of the accuracy research of the method meet requirements for the permissible limits of ±10.0%. Taking into account the technical capabilities of the Ukrainian producers and a diverse list of excipients used in the manufacture of the drug, the spectrophotometric quantitative determination of paracetamol tablets by specific absorbance is recommended to use with the permissible limits of ±10.0%. The prognosis of the total uncertainty of the analysis results is consistent with requirements to the maximum permissible uncertainty of the analysis Δ10.0%As = 2.6 ≤ max Δ10.0%As= 3.2% and with results of the 3rd round of the Professional Testing Programme (PTP) of “Pharma-test” laboratories in the system of the State Inspection for Medication Quality Control of the Ministry of Public Health of Ukraine.