3D-printed Microfluidic Device Research Articles

A multistep immunoassay on a 3D-printed capillarity-driven microfluidic device for point-of-care diagnostics

Although efficient diagnostic testing often relies on centralized laboratories, rapid diagnostics at the point-of-care (POC) are increasingly investigated to provide an affordable and easy-to-use alternative that requires minimal external equipment. Implementing complex assays on such POC devices is challenging, and existing methods such as paper-based analytical devices suffer from laborious and heterogeneous fabrication. 3D printing is a promising technology to enable the single-step fabrication of such diagnostic devices. We previously reported monolithic, 3D-printed microfluidics driven by capillarity, and used the platform to implement a proof-of-concept enzyme-linked immunosorbent assay (ELISA) for immunoglobulin E (IgE) detection. In this study, we aimed to improve the time-to-result and detection limit of this approach by (i) designing a second-generation 3D-printed device and (ii) further optimizing the IgE ELISA. Herein, we selected horseradish peroxidase for faster and more sensitive signal amplification compared to the first-generation device, for which we adapted the microfluidic device design to allow sequential flow while using separated reagent channels for each assay step. To implement the ELISA on this new design, we decreased reagent volumes and incubation times to be compatible with POC, and then we translated this assay stepwise from microtiter plates to the completely 3D-printed, second-generation device. Overall, this work gives additional insight into the biocompatibility of these 3D-printed devices, as well as demonstrates the huge potential of powder bed 3D printing to fabricate POC diagnostic devices.

Simultaneous detection of dopamine and ascorbic acid by using a thread-based microfluidic device and multiple pulse amperometry.

This study presents a novel approach for the simultaneous detection of ascorbic acid (AA) and dopamine (DA) using an affordable and user-friendly microfluidic device. Microfluidic devices, when combined with electrochemical detectors like screen-printed electrodes (SPEs), offer numerous advantages such as portability, high sample throughput, and low reagent consumption. In this study, a 3D-printed microfluidic device called a μTED was developed, utilizing textile threads as microfluidic channels and an unmodified SPE as the amperometric detector. The method employed multiple pulse amperometry (MPA) with carefully selected potential values (+0.65 V and -0.10 V). The reduction current signals generated by dopamine o-quinone were used to calculate a correction factor for the oxidation signals of ascorbic acid, enabling simultaneous quantification. The developed microfluidic device ensured a stable flow rate of the carrier solution at 1.19 μL s-1, minimizing the consumption of samples and reagents (injection volume of 2.0 μL). Under the optimized experimental conditions, a linear range from 50 to 900 μmol L-1 was achieved for both DA and AA. The obtained sensitivities were 2.24 μA L mmol-1 for AA and 5.09 μA L mmol-1 for DA, with corresponding limits of detection (LOD) of 2.60 μmol L-1 and 1.54 μmol L-1, respectively. To confirm the effectiveness of the proposed method, it was successfully applied to analyze AA and DA in a commercial blood serum sample spiked at three different concentration levels, with a medium recovery rate of 70%. Furthermore, the MPA technique demonstrated its simplicity by enabling the simultaneous determination of AA and DA without the need for prior separation steps or the use of chemically modified electrodes.

Study of the Influence of the Dielectrophoretic Force on the Preferential Growth of Bacterial Biofilms in 3D Printed Microfluidic Devices

Understanding the effect of different electric potentials upon the preferential formation of biofilms inside microfluidic devices could represent a step forward in comprehending the mechanisms that govern biofilm formation and growth. 3D printed microfluidic devices were used to investigate the influence of the dielectrophoretic forces on the formation and growth of Staphylococcus aureus ATCC 25923 biofilms. Bacterial suspensions of 2.5 McF were pushed through microfluidic channels while simultaneously applying various potential differences between 10 and 60 V. The overall electric field distribution within the channel was simulated using the COMOSL software. The effect of the electric potential variation on the preferential biofilm formation was determined using an adjusted microtiter plate technique, as well as a qualitative method, Scanning Electron Microscopy (SEM). SEM images were used to describe the morphology of the biofilm surface. The conclusions show that the dielectrophoretic forces, resulting due to inhomogeneity of the electric field, have more visible effects upon the cells up to 40 V. Above this magnitude, due to a more homogenous distribution of the electric field, the formation and growth of the biofilm become more uniform. At around 60 V, the distance between the high electric gradient regions decreases, leading to an almost uniform distribution of the electric field and, therefore, to a shift from dielectrophoretic to electrophoretic forces acting upon the bacterial cells.

Surface Modification of 3D Printed Microfluidic Devices for Controlled Wetting in Two-Phase Flow.

Microfluidic devices (MFDs) printed in 3-D geometry using digital light projection to polymerize monomers often have surfaces that are not as hydrophobic as MFDs made from polydimethylsiloxane. Droplet microfluidics in these types of devices are subject to droplet adhesion and aqueous spreading on less hydrophobic MFD surfaces. We have developed a post-processing technique using hydrophobic monomers that renders the surfaces of these devices much more hydrophobic. The technique is fast and easy, and involves flowing monomer without initiator into the channels and then exposing the entire device to UV light that generates radicals from the initiator molecules remaining in the original 3-D polymerization. After treatment the channels can be cleared and the surface is more hydrophobic, as evidenced by higher contact angles with aqueous droplets. We hypothesize that radicals generated near the previously printed surfaces initiate polymerization of the hydrophobic monomers on the surfaces without bulk polymerization extending into the channels. The most hydrophobic surfaces were produced by treatment with an alkyl acrylate and a fluorinated acrylate. This technique could be used for surface treatment with other types of monomers to impart unique characteristics to channels in MFDs.

Electroosmotic flow in fused deposition modeling (FDM) 3D-printed microchannels.

Electroosmotic flow (EOF) was determined in tridimensional (3D)-printed microchannels with dimensions smaller than 100µm. Fused deposition modeling 3D printing using thermoplastic filaments of PETG (polyethylene terephthalate glycol), PLA (polylactic acid), and ABS (acrylonitrile butadiene styrene) were used to fabricate the microchannels. The current monitoring method and sodium phosphate solutions at different pH values (3-10) were used for the EOF mobility (µEOF ) measurements, which ranged from 2.00×10-4 to 12.52×10-4 cm2 V-1 s-1 . The highest and the smallest µEOF were obtained for the PLA and PETG microchannels, respectively. Adding the cationic surfactant cetyltrimethylammonium bromide to the sodium phosphate solution caused EOF direction reversion in all the studied microchannels. The obtained results can be interesting for developing 3D-printed microfluidic devices, in which EOF is relevant.

Easy and Affordable: A New Method for the Studying of Bacterial Biofilm Formation.

Bacterial biofilm formation (BBF) proves itself to be in the spotlight of microbiology research due to the wide variety of infections that it can be associated with, the involvement in food spoilage, industrial biofouling and perhaps sewage treatment. However, BBF remains difficult to study due to the lack of standardization of the existing methods and the expensive equipment needed. We aim to describe a new inexpensive and easy to reproduce protocol for a 3D-printed microfluidic device that can be used to study BBF in a dynamic manner. We used the SolidWorks 3D CAD Software (EducationEdition 2019-2020, Dassault Systèmes, Vélizy-Villacoublay, France) to design the device and the Creality3D Ender 5 printer (Shenzhen Creality 3D Technology Co., Ltd., Shenzhen, China) for its manufacture. We cultivated strains of Enterococcus faecalis, Staphylococcus aureus, Klebsiella pneumoniae and Pseudomonas aeruginosa. For the biofilm evaluation we used optical coherence tomography (OCT), scanning electron microscopy (SEM), Fourier Transform Infrared (FTIR) spectroscopy and crystal violet staining technique. Based on the analysis, Enterococcus faecalis seems to produce more biofilm in the first hours while Pseudomonas aeruginosa started to take the lead on biofilm production after 24 h. With an estimated cost around €0.1285 for one microfluidic device, a relatively inexpensive and easy alternative for the study of BBF was developed.

Non-covalent π–π functionalized Gii-senseⓇ graphene foam for interleukin 10 impedimetric detection

Monitoring Interleukin 10 (IL-10) is essential for understanding the vast responses of T-cells in cancer, autoimmunity, and internal homeostasis after physical stress. However, current diagnostic methods are complex and more focused on medical screening rather than point-of-care monitoring. Biosensors based on graphene's conductivity and flexibility are attractive to offer simple single-use and reduced handling. However, oxidation of its carbon lattice to develop functional moieties for biomolecule immobilization cuts down its electronic conductivity potential. In this work, the authors present a microfluidic lab-on-chip device for simple impedimetric monitoring of IL-10 based on graphene foam (GF) flexible electrodes. Graphene's structure was maintained by employing π–π non-covalent functionalization with pyrene carboxylic acid (PCA). Impedimetric measurements could be performed in low ionic strength phosphate-buffered saline (LI-PBS). The PCA-antibody modification showed to endure the incubation, measurement, and washing processes performed in the microfluidic device. Electrode modification and measurements were characterized by, electrochemical impedance spectroscopy (EIS), contact angle, and scanning electron microscopy. From the contact angle results, we found that the wettability of the graphene surface increased gradually after each modification step. Detection measurements performed in the 3D-printed microfluidic device showed a linear response between 10 fg/mL to 100 fg/mL with a limit of detection (LOD) of 7.89 fg/mL in artificial saliva. With these features, the device was used to quantify IL-10 samples by the standard addition method for 10 fg and 50 fg with recoveries between 82% and 99%. Specificity was evaluated towards interleukin 6, TNF-⍺ and bovine serum albumin.

In-House Innovative "Diamond Shaped" 3D Printed Microfluidic Devices for Lysozyme-Loaded Liposomes.

Nanotechnology applications have emerged as one of the most actively researched areas in recent years. As a result, substantial study into nanoparticulate lipidic systems and liposomes (LPs) has been conducted. Regardless of the advantages, various challenges involving traditional manufacturing processes have hampered their expansion. Here, the combination of microfluidic technology (MF) and 3D printing (3DP) digital light processing (DLP) was fruitfully investigated in the creation of novel, previously unexplored "diamond shaped" devices suitable for the production of LPs carrying lysozyme as model drug. Computer-aided design (CAD) software was used designing several MF devices with significantly multiple and diverse geometries. These were printed using a high-performance DLP 3DP, resulting in extremely high-resolution chips that were tested to optimize the experimental condition of MF-based LPs. Monodisperse narrow-sized lysozyme-loaded PEGylated LPs were produced using in-house devices. The developed formulations succumbed to stability tests to determine their consistency, and then an encapsulation efficacy (EE) study was performed, yielding good findings. The in vitro release study indicated that lysozyme-loaded LPs could release up to 93% of the encapsulated cargo within 72 h. Therefore, the proficiency of the association between MF and 3DP was demonstrated, revealing a potential growing synergy.

Microfluidic Continuous Flow DNA Fragmentation based on a Vibrating Sharp-tip.

Fragmentation of DNA into short fragments is of great importance for detecting and studying DNAs. Current microfluidic methods of DNA fragmentation are either inefficient for generating small fragments or rely on microbubbles. Here, we report a DNA fragmentation method in a 3D-printed microfluidic device, which allows efficient continuous flow fragmentation of genomic DNAs without the need for microbubbles. This method is enabled by localized acoustic streaming induced by a single vibrating sharp-tip. Genomic DNAs were fragmented into 700 to 3000 bp fragments with a low power consumption of ~140 mW. The system demonstrated successful fragmentation under a wide range of flow rates from 1 to 50 μL/min without the need for air bubbles. Finally, the utility of the continuous DNA fragmentation method was demonstrated to accelerate the DNA hybridization process for biosensing. Due to the small footprint, continuous flow and bubble-free operation, and high fragmentation efficiency, this method demonstrated great potential for coupling with other functional microfluidic units to achieve an integrated DNA analysis platform.

3D-printed microfluidic thread device with integrated detector: A green and portable tool for amperometric detection of fungicide benzovindiflupyr in forensic samples

The illegal sale of pesticides is a current problem around the world and has been increasing year by year. Thus, the development of novel, fast and simple analytical methods for screening and quantification of these compounds is a relevant matter. Here we demonstrate, for the first time, the possibility of electrochemical detection of fungicide benzovindiflupyr (BENZO) using a microfluidic thread electroanalytical device (µTED) coupled to amperometric detection. The µTED used here contains an integrated three-electrode detector and was fabricated by 3D printing using conductive (carbon black/polylactic acid, CB-PLA) and non-conductive (acrylonitrile butadiene styrene, ABS) filaments. It allows a large-scale and reproducible production of this analytical platform, making its implementation easier and accessible to other users. Using the optimal parameters, this 3D-printed µTED (3D-µTED) provided a linear response for BENZO in the concentration range from 3.0 to 120.0 µmolL−1. The limit of detection (LOD) obtained was 0.44 µmolL−1. The 3D-µTED was successfully used for the screening and quantification of BENZO in smuggled products. Screening results were compared with gas-chromatography and presented great concordance. The results found in the quantitative analyses showed good accuracy (values between 98 and 103 % of those stated on the product labels) and precision (relative standard deviations < 5 %), evidencing the great potential of this microfluidic platform in forensic sciences.

Investigation of anti-cancer effects of new pyrazino[1,2-a]benzimidazole derivatives on human glioblastoma cells through 2D in vitro model and 3D-printed microfluidic device

AimsRecent studies show targeted therapy of new pyrazino[1,2-a]benzimidazole derivatives with COX-II inhibitory effects on different cancer cells. This study aimed to investigate 2D cell culture and 3D spheroid formation of glioblastoma multiforme (GBM) cells using a microfluidic device after exposure to these compounds. Main methodsAfter isolating astrocytes from human GBM samples, IC50 of 2,6-dimethyl pyrazino[1,2-a]benzimidazole (L1) and 3,4,5-trimethoxy pyrazino[1,2-a]benzimidazole (L2) were determined as 13 μM and 85 μM, respectively. Then, in all experiments, cells were exposed to subtoxic concentrations of L1 (6.5 μM) and L2 (42.5 μM), which were ½IC50. In the following, in two phases, cell cycle, migration, and gene expression through 2D cell culture and tumor spheroid formation ability using a 3D-printed microfluidic chip were assessed. Key findingsThe obtained results showed that both compounds have positive effects in reducing G2/M cell population and GBM cell migration. Furthermore, real-time gene expression data showed that L1 and L2 significantly impact the upregulation of P21 and P53 and down-regulation of cyclin D1, MMP2, and MMP9. On the other hand, GBM spheroids exposed to L1 and L2 become smaller with fewer live cells. SignificanceOur data on human isolated astrocyte cells in 2D and 3D cell culture conditions showed that L1 and L2 compounds could reduce GBM cells' invasion by controlling gene expressions associated with migration and proliferation. Moreover, designing microfluidic platform and related cell culture protocols facilitates the broad screening of 3D multicellular tumor spheroids derived from GBM tumor biopsies and provides effective drug development for brain gliomas.

Centrifugation-Assisted Three-Dimensional Printing of Devices Embedded with Fully Enclosed Microchannels.

The challenges in reliably removing the sacrificial material from fully enclosed microfluidic channels hinder the use of three-dimensional (3D) printing to create microfluidic devices with intricate geometries. With advances in printer resolution, the etching of sacrificial materials from increasingly smaller channels is poised to be a bottleneck using the existing techniques. In this study, we introduce a microfabrication approach that utilizes centrifugation to effortlessly and efficiently remove the sacrificial materials from 3D-printed microfluidic devices with densely packed microfeatures. We characterize the process by measuring the etch rate under different centrifugal forces and developed a theoretical model to estimate process parameters for a given geometry. The effect of the device layout on the centrifugal etching process is also investigated. We demonstrate the applicability of our approach on devices fabricated using inkjet 3D printing and stereolithography. Finally, the advantages of the introduced approach over commonly used injection-based etching of sacrificial material are experimentally demonstrated in direct comparisons. A robust method to postprocess additively manufactured geometries composed of intricate microfluidic channels can help utilize both the large printing volume and high spatial resolution afforded by 3D printing in creating a variety of devices ranging from scaffolds to large-scale microfluidic assays.

Time-efficient fabrication method for 3D-printed microfluidic devices

Recent developments in 3D-printing technology have provided a time-efficient and inexpensive alternative to the fabrication of microfluidic devices. At present, 3D-printed microfluidic systems face the challenges of post-processing, non-transparency, and being time consuming, limiting their practical application. In this study, a time-efficient and inexpensive fabrication method was developed for 3D-printed microfluidic devices. The material for 3D-printed microfluidic chips is Dowsil 732, which is used as a sealant or encapsulant in various industries. The curing time and surface hydrophobicity of the materials were evaluated. The results indicated that the surface of Dowsil 732 is hydrophilic. An optimization model of the direct ink writing method is proposed to establish a time-efficient and accurate fabrication method for microfluidic devices. The results indicate that the optimization model can effectively describe the change trend between printing speed, printing pressure, and channel wall accuracy, and the model accuracy rate exceeds 95%. Three examples—a micromixer, concentration gradient generator, and droplet generator—were printed to demonstrate the functionality and feasibility of the fabrication method.

Stereolithography based 3D-printed microfluidic device with integrated electrochemical detection

We demonstrate for the first time the conception of a reusable stereolithography (SLA) based 3D printed separation device with integrated electrodes for electrochemical (EC) detection. For this purpose, a new lab-made working electrode (WE) was developed by 3D-printing a side wall microchannel along the separation microchannel and by inserting a graphite/ resin (C/resin) composite within this side wall microchannel. The 3D-printing conditions of the electrochemical microfluidic device (EMD) as well as the electrode composition, geometry, and integration were optimized for easy and low-cost fabrication (no need for a clean room) and suitable electrochemical response. The electrochemical characterization of the material formulation was first performed in bulk format (disk-shaped, 2 mm diameter) and then moved to the micrometric scale reaching electrode width of 172 ± 12 µm. The integrated electrodes were characterised by voltammetry, and the response linearity of the transducer material was investigated by square wave voltammetry (SWV) using ferrocene methanol (FcMeOH). The C/resin electrode led to a good repeatability (RSD of ± 2% (n = 3) in the peak current values), linear response (R2 = 0.995) in the 15 − 1000 µM range, allowing for limits of detection and quantification of 4.5 µM and 15 µM, respectively. In order to develop a complex analytical device, the interest of this dedicated device was evidenced in a hydrodynamic state condition with Ru(NH3)63+as a proof-of-concept, ranging from 0 to 55 µL min−1 mimicking the electroosmotic flow conditions, which is a bulk liquid motion very common in electrically driven separation techniques .

Development of Dexamethasone Loaded Nanomicelles Using a 3d Printed Microfluidic Device for Ocular Drug Delivery Applications

Development of Dexamethasone Loaded Nanomicelles Using a 3d Printed Microfluidic Device for Ocular Drug Delivery Applications

3D-Printed microfluidic device for protein purification in batch chromatography.

Modern 3D printers enable not only rapid prototyping, but also high-precision printing-microfluidic devices with channel diameters of just a few micrometres can now be readily assembled using this technology. Such devices offer a myriad of benefits (including miniaturization) that significantly reduce sample and buffer volumes and lead to lower process costs. Although such microfluidic devices are already widely used in the field of biotechnology, there is a lack of research regarding the potential of miniaturization by 3D-printed devices in lab-scale chromatography. In this study, the efficacy of a 3D-printed microfluidic device which provides a substantially lower dead-volume compared to established chromatography systems is demonstrated for batch purification applications. Furthermore, this device enables straightforward integration of various components (such as microfluidic valves and chromatographic units) in an unprecedentedly flexible fashion. Initial proof-of-concept experiments demonstrate successful gradient elution with bovine serum albumin (BSA), and the purification of a pharmaceutically relevant IgG monoclonal antibody (mAb).

A 3D-Printed Microfluidic Device for qPCR Detection of Macrolide-Resistant Mutations of Mycoplasma pneumoniae.

Mycoplasma pneumonia (MP) is a common respiratory infection generally treated with macrolides, but resistance mutations against macrolides are often detected in mycoplasma pneumoniae in China. Rapid and accurate identification of mycoplasma pneumoniae and its mutant type is necessary for precise medication. This paper presents a 3D-printed microfluidic device to achieve this. By 3D printing, the stereoscopic structures such as microvalves, reservoirs, drainage tubes, and connectors were fabricated in one step. The device integrated commercial polymerase chain reaction (PCR) tubes as PCR chambers. The detection was a sample-to-answer procedure. First, the sample, a PCR mix, and mineral oil were respectively added to the reservoirs on the device. Next, the device automatically mixed the sample with the PCR mix and evenly dispensed the mixed solution and mineral oil into the PCR chambers, which were preloaded with the specified primers and probes. Subsequently, quantitative real-time PCR (qPCR) was carried out with the homemade instrument. Within 80 min, mycoplasma pneumoniae and its mutation type in the clinical samples were determined, which was verified by DNA sequencing. The easy-to-make and easy-to-use device provides a rapid and integrated detection approach for pathogens and antibiotic resistance mutations, which is urgently needed on the infection scene and in hospital emergency departments.

3D Printing of Superhydrophobic Objects with Bulk Nanostructure.

The rapid development of 3D printing (or additive manufacturing) technologies demands new materials with novel properties and functionalities. Superhydrophobic materials, owing to their ultralow water adhesion, self-cleaning, anti-biofouling, or superoleophilic properties are useful for myriad applications involving liquids. However, the majority of the methods for making superhydrophobic surfaces have been based on surface functionalization and coatings, which are challenging to apply to 3D objects. Additionally, these coatings are vulnerable to abrasion due to low mechanical stability and limited thickness. Here, a new materials concept and methodology for 3D printing of superhydrophobic macroscopic objects with bulk nanostructure and almost unlimited geometrical freedom is presented. The method is based on a specific ink composed of hydrophobic (meth)acrylate monomers and porogen solvents, which undergoes phase separation upon photopolymerization to generate inherently nanoporous and superhydrophobic structures. Using a desktop Digital Light Processing printer, superhydrophobic 3D objects with complex shapes are demonstrated, with ultralow and uniform water adhesion measured with scanning droplet adhesion microscopy. It is shown that the 3D-printed objects, owing to their nanoporous structure throughout the entire volume, preserve their superhydrophobicity upon wear damage. Finally, a superhydrophobic 3D-printed gas-permeable and water-repellent microfluidic device and a hierarchically structured 3D-printed super-oil-absorbent are demonstrated.

Portable all-in-one automated microfluidic system (PAMICON) with 3D-printed chip using novel fluid control mechanism

State-of-the-art microfluidic systems rely on relatively expensive and bulky off-chip infrastructures. The core of a system—the microfluidic chip—requires a clean room and dedicated skills to be fabricated. Thus, state-of-the-art microfluidic systems are barely accessible, especially for the do-it-yourself (DIY) community or enthusiasts. Recent emerging technology—3D-printing—has shown promise to fabricate microfluidic chips more simply, but the resulting chip is mainly hardened and single-layered and can hardly replace the state-of-the-art Polydimethylsiloxane (PDMS) chip. There exists no convenient fluidic control mechanism yet suitable for the hardened single-layered chip, and particularly, the hardened single-layered chip cannot replicate the pneumatic valve—an essential actuator for automatically controlled microfluidics. Instead, 3D-printable non-pneumatic or manually actuated valve designs are reported, but their application is limited. Here, we present a low-cost accessible all-in-one portable microfluidic system, which uses an easy-to-print single-layered 3D-printed microfluidic chip along with a novel active control mechanism for fluids to enable more applications. This active control mechanism is based on air or gas interception and can, e.g., block, direct, and transport fluid. As a demonstration, we show the system can automatically control the fluid in microfluidic chips, which we designed and printed with a consumer-grade 3D-printer. The system is comparably compact and can automatically perform user-programmed experiments. All operations can be done directly on the system with no additional host device required. This work could support the spread of low budget accessible microfluidic systems as portable, usable on-the-go devices and increase the application field of 3D-printed microfluidic devices.

Manufacturing of 3D-Printed Microfluidic Devices for the Synthesis of Drug-Loaded Liposomal Formulations.

Microfluidic technique has emerged as a promising tool for the production of stable and monodispersed nanoparticles (NPs). In particular, this work focuses on liposome production by microfluidics and on factors involved in determining liposome characteristics. Traditional fabrication techniques for microfluidic devices suffer from several disadvantages, such as multistep processing and expensive facilities. Three-dimensional printing (3DP) has been revolutionary for microfluidic device production, boasting facile and low-cost fabrication. In this study, microfluidic devices with innovative micromixing patterns were developed using fused deposition modelling (FDM) and liquid crystal display (LCD) printers. To date, this work is the first to study liposome production using LCD-printed microfluidic devices. The current study deals with 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) liposomes with cholesterol (2:1) prepared using commercial and 3D-printed microfluidic devices. We evaluated the effect of microfluidic parameters, chip manufacturing, material, and channel design on liposomal formulation by analysing the size, PDI, and ζ-potential. Curcumin exhibits potent anticancer activity and it has been reported that curcumin-loaded liposomes formulated by microfluidics show enhanced encapsulation efficiency when compared with other reported systems. In this work, curcumal liposomes were produced using the developed microfluidic devices and particle sizing, ζ-potential, encapsulation efficiency, and in vitro release studies were performed at 37 °C.