3D Nanofiber Research Articles

A highly efficient adsorbent constructed by the in situ assembly of Zeolitic imidazole framework-67 on 3D aramid nanofiber aerogel scaffold

• ZIF-67 was in situ assembled onto ANF aerogel to get 3D monolith adsorbent. • ANF/ZIF-67 aerogel showed stable and competitive structural features. • ANF/ZIF-67 aerogel demonstrated high adsorption capacity for Cu 2+ and Cr 6+ . • ANF/ZIF-67 aerogel displayed excellent adsorption capacity for CV and MO. • The adsorption capacity maintained up to 80% after five times of usage. The fabrication of a high-efficiency adsorbent, as a promising candidate for removing metal ions and organic dyes from aqueous solution, is a research hotspot but remains challenging. Aramid nanofibers (ANFs) are emerging building blocks that have realized multifunctional applications in various fields because of their intrinsic mechanical and chemical stability. In this research, three-dimensional (3D) ANF aerogel was assembled and applied in for in situ assembly of zeolitic imidazole framework-67 (ZIF-67). The aim, was to fully exploit the structural advantages of this composite’s ultra-low bulk density, large specific surface area, and high porosity, as well as provide more available adsorption sites by ZIF-67 polyhedrons, to facilitate pollutant adsorption from waste water. The prepared 3D ANF/ZIF-67 composite aerogel possessed favorable adsorption performance for heavy metal ions (250.95 and 183.29 mg/g for Cu 2+ and Cr 6+ , respectively) and organic dyes (199.36 and 65.98 mg/g for CV and MO, respectively), greatly exceeding that of the corresponding 3D ANF aerogel. Desorption experiments further demonstrated that up to 80% adsorption performance for heavy metal ions and organic dyes could be maintained after five times of usage. Hence, this research can provide new insights into the preparation of highly efficient adsorbents for water treatment.

Flexible, Ultra‐Light, and 3D Designed White‐Light‐Emitting Nanofiber Aerogel

AbstractWhite light could be achieved by controlling the energy transfer (ET) of different fluorescent molecules in the donor–acceptor system. However, it is still a huge challenge to inhibit the excessive ET efficiency and achieve a wide range of white‐light‐emitting (WLE) spectrum. In this study, the authors develop a simple and efficient method to prepare a fluorescent nanofiber aerogel (NFA). The 3D nanofiber network is conducive to the uniform dispersion of fluorescent molecules and can effectively inhibit the excessive ET between donor and acceptor. By simply adjusting the mass ratios of blue and orange fluorescent nanofibers in the NFA to 3:2, a uniform white light (0.322,0.305) that covered the entire visible region is obtained. This WLE‐NFA has an ultra‐light volume density of 6.47 mg cm–3 and its quantum yield is up to 27%. In addition, the fluorescent NFA can also be processed into different 3D shapes and integrated luminous patterns through various designs of the freezing mold. This brand‐new fluorescent NFA can overcome the poor plasticity of traditional fluorescent nanofiber films, which is expected to provide a new thought for developing next‐generation flexible WLE materials.

Bio nanocomposites of graphene oxide with carboxymethyl guargum: fabrication and characterization and application for type 1 diabetes

Islet cells transplantation has limitations like low survivability, which can be overcome by using extracellular matrix mimicking three-dimensional (3D) scaffolds, which supports the growth and proliferation of seeded cells. This study was aimed to investigate the role of novel 3D carboxymethyl guargum (CMGG) nanocomposite with reduced graphene oxide (rGO) for proliferation of pancreatic islet cells (RIN-5F) and rate of insulin secretion of RIN-5F cells. Scanning electron microscope and Fourier transform infrared results have demonstrated good porosity and the chemical interactions between CMGG and rGO. Mechanical testing and thermogravimetric analysis of nanofibers have shown good tensile strength and thermal stability with rGO in the nanocomposite. These scaffolds demonstrated in vitro biocompatibility with acceptable ranges of biodegradability and hemocompatibility. The in vitro cell proliferation and viability of RIN-5F cells on 3D CMGG nanofibers have significantly increased compared to two-dimensional (2D) cell control. Moreover, the glucose dependent insulin secretion of RIN-5F cells on CMGG nanocomposite has significantly increased upto 4–5 folds than cells on 2D cell control. The biomaterials used in this 3D nanofiber scaffold have shown to be biodegradable and hemocompatible and can be a promising platform for the proliferation and secretion of insulin from beta cells and can be effectively used in transplantation type-1 diabetes.

3D Self-Supported Nitrogen-Doped Carbon Nanofiber Electrodes Incorporated Co/CoOx Nanoparticles: Application to Dyes Degradation by Electro-Fenton-Based Process.

We developed free-standing nitrogen-doped carbon nanofiber (CNF) electrodes incorporating Co/CoOx nanoparticles (NPs) as a new cathode material for removing Acid Orange 7 (AO7; a dye for wool) from wastewater by the heterogeneous electro-Fenton reaction. We produced the free-standing N-doped CNF electrodes by electrospinning a polyacrylonitrile (PAN) and cobalt acetate solution followed by thermal carbonation of the cobalt acetate/PAN nanofibers under a nitrogen atmosphere. We then investigated electro-Fenton-based removal of AO7 from wastewater with the free-standing N-doped-CNFs-Co/CoOx electrodes, in the presence or not of Fe2+ ions as a co-catalyst. The electrochemical analysis showed the high stability of the prepared N-doped-CNF-Co/CoOx electrodes in electrochemical oxidation experiments with excellent degradation of AO7 (20 mM) at acidic to near neutral pH values (3 and 6). Electro-Fenton oxidation at 10 mA/cm2 direct current for 40 min using the N-doped-CNF-Co/CoOx electrodes loaded with 25 wt% of Co/CoOx NPs led to complete AO7 solution decolorization with total organic carbon (TOC) removal values of 92.4% at pH 3 and 93.3% at pH 6. The newly developed N-doped-CNF-Co/CoOx electrodes are an effective alternative technique for wastewater pre-treatment before the biological treatment.

Conjugate Electrospun 3D Gelatin Nanofiber Sponge for Rapid Hemostasis (Adv. Healthcare Mater. 20/2021)

Nanofiber Sponges In article number 2100918, Jinglei Wu, Xiumei Mo, and co-workers develop a novel conjugate electrospinning strategy to prepare a 3D nanofiber sponge with low density, high surface area, compressibility. This method can be widely used in the preparation of various polymer nanofiber sponges. The obtained gelatin nanofiber sponge holds great potential as an absorbable hemostatic agent for rapid hemostasis.

Development of a 3D Cell Culture System Using Amphiphilic Polydepsipeptides and Its Application to Hepatic Differentiation.

Various three-dimensional (3D) culture systems are available to provide more accurate in vivo mimicry than two-dimensional (2D) cultures. Synthetic and/or xeno-free biomaterials are desired, as they would provide lower batch-to-batch variability and high repeatability. Here, we introduce a 3D culture system using nanofibers composed of an amphiphilic polydepsipeptide-based polymer named HYDROX, which turns into 3D nanofibers after hydration. Our system produces a large amount of cell aggregates and requires only the seeding of a cell mixture. In addition, cells cultured with HYDROX can be collected with only a centrifugation procedure, and analytical assays can then be performed. Here, we applied HYDROX to hepatic differentiation from induced pluripotent stem cells. The cells cultured with HYDROX formed aggregates and HYDROX strongly promoted hepatic differentiation and maturation in terms of functions such as the positive ratio of alpha-1 antitrypsin, the production of albumin, the cytochrome P450 (CYP) 3A4 activity, and the low-density-lipoprotein uptake ability. In addition, primary human hepatocytes cultured with HYDROX showed significantly improved CYP3A4 gene expression and activity. The viscoelasticity and stiffness of HYDROX can be modulated by varying the concentration of the synthetic polymer, thereby providing a suitable microenvironment for the differentiation of cells with various characteristics toward a target cell type. Our findings demonstrated that HYDROX is a promising biomaterial for 3D cultures in research fields ranging from basic cell research to drug discovery.

Peculiar micro and nano cell morphology of PBT/PTFE nanofibrillated composite foams of supercritical CO2 foaming induced by in-situ formed 3D PTFE nanofiber networks

This study investigates the role of polytetrafluoroethylene (PTFE) nanofibers on the peculiar micro and nano foaming behavior of polybutylene terephthalate (PBT) before and after crystallization. In-situ PBT/PTFE nanofibrillated composites were prepared by melt compounding in torque rheometer. A physical network of high aspect ratio PTFE nanofiber is formed as the result of the fibers splitting and entanglement demonstrated by scanning electron microscope (SEM). In molten PBT, the formation of the nanofiber networks significantly increases the melt viscoelastic properties and promotes cell nucleation. As a result, high expansion ratio foams with average cell size of 13 μm were achieved in the melting PBT, which has some guiding significance for the formation of microcellular foam in extrusion foaming process. In addition, the crystallization process and crystallization morphology were investigated by differential scanning calorimeter (DSC), polarizing microscope (POM) and SEM. The crystallization kinetics of PBT were promoted due to the more crystallization nucleation interface provided by the high specific surface area of PTFE nanofibers. In the crystallization process of PBT/PTFE nanofibrillated composites, the PBT molecular chain first crystallized along the surface of PTFE nanofiber to form shish-kebab crystalline structure, and then the PBT molecular chain away from PTFE nanofibers crystallized. Consequently, the shish-kebab crystalline structure and the two-step crystallization induced formation of the peculiar oriented micro and nano cells. The research could present a facile and flexible method to regulate the cell morphology and expand the application of PBT foam.

Construction of a multifunctional 3D nanofiber aerogel loaded with ZnO for wound healing

Bacterial infection and severe wound inflammation are two primary harmful problems that bring harm to the human body and may cause death when a large-scale skin defect occurs. Thus, developing an effective and quick wound healing strategy for curing skin damage and trauma is vital. This study has developed a multifunctional PLA/gelatin/ZnO nanofiber aerogel with a three-dimensional structure through electrospinning and freeze-drying technology for wound healing. It has validated that the nanofiber aerogel has an excellent antibacterial property and biocompatibility. Meanwhile, benefiting from its three-dimensional nanofiber structure, the PLA/gelatin/ZnO nanofiber aerogel possesses good water absorption and air permeability. In vivo experiments have determined that the PLA/gel/ZnO nanofiber aerogel scaffolds effectively promote skin infection’s wound healing and enhance angiogenesis that is practical with increasing ZnO concentration.

Cover Image, Volume 69, Issue 11

Cover Illustration: Maximum intensity projection image of src–kinase associated protein 2 (SKAP2) – deficient oligodendrocytes cultivated for 48 hours on PLL–FITC coated 3D nanofibers (green) and stained for myelin basic protein (red). SKAP2–deficient oligodendrocytes displayed higher complexity with more processes as well as increased total process length per cell compared to wildtype cells. (See Ghelman, J., et al, https://doi.org/10.1002/glia.24066.)

All MOF-derived-carbon material-based integrated electrode constructed by carbon nanosheet sulfur host and Fe microparticles with carbon nanofiber network interlayer for lithium–sulfur batteries

Lithium–sulfur (Li–S) batteries are promising future-generation portable electronic devices due to the outstanding energy density of S. However, the serve problems of Li–S batteries originating from the intrinsic poor conductivity of S and the dissolution and diffusion of dissoluble polysulfides in electrolytes are a major limitation. Herein, we demonstrated an all metal–organic framework (MOF)-derived carbon material-based integrated sulfur cathode, which was constructed with 3D carbon nanofiber network with Fe microparticles (MIL1000) as interlayer on a polypropylene (PP) separator and 2D nanosheets (MIL900-H) as S host. Notably, the MIL900-H carbon nanosheets with many mesopores and doping oxygen possessed lithium-ion pathways and chemical adsorption of soluble polysulfides, and the MIL1000 interlayer could chemically adsorb, physically block polysulfide, and facilitate the kinetics of the cathode reaction. As a result, the integrated electrode that avoided the use of a metal current collector exhibited a high initial capacity of 1244.6 mAh/g at 0.1 C, fast Li2S nucleation, and excellent cycle stability with the capacity retention of 69.3% after 500 cycles at 1 C. Therefore, this rationale strategy of constructing an all MOF-derived-carbon material-based integrated electrode can be extended to other abundant MOF-derived materials for high energy density Li–S batteries.

Free-standing hollow carbon nanofibers scaffold with spherical nanocavities and lithiophilic N/ZnO heteroatoms as stable dendrite-free lithium metal anode

Metallic Li as substitutional anode materials for high specific capacity lithium-ion batteries (LIBs) has been attached great importance. However, two major problems including serve Li dendrites growth and large volume change need to be settled before it can be commercialized. Herein, a 3D porous hollow carbon nanofibers (MC@HCNFs) with high electron transport rate and low regional current density polarization are designed, which are comprised of MOF-derived N/ZnO co-doped carbon frameworks and embedded carbon nanotubes. The spherical cavities within MC@HCNFs formed by the high-temperature decomposition of MOFs nanoparticles can provide abundant active space for Li metal nucleation and deposition to alleviate the infinite volume expansion, and more importantly regulate homogenous Li ions flow and preferential Li metal deposition to suppress the Li dendrites growth due to the presence of lithiophilic N/ZnO heteroatoms on the surface. As a Li metal carrier, MC@HCNFs displays stable Li platting/stripping for ultralong life spans of 2000 h with lower overpotentials at 1.0 mA cm−2 respectively, which also demonstrate excellent cycle and rate performances when used in LiFePO4-based full cells.

Enhancing Cellular Infiltration on Fluffy Polyaniline-Based Electrospun Nanofibers.

Despite the unique properties of polyaniline (PANI), the processability of this smart polymer is associated with challenges. Particularly, it is very difficult to prepare PANI nanofibers due to poor solubility, high charge density, and rigid backbone. The most common approach for solving this problem is blending PANI with a carrier polymer. Furthermore, the major limitations of nanofibers for tissue engineering applications are their low porosity and two-dimensional (2D) structure. In this study, conductive nanofibers were fabricated through electrospinning of PANI/poly(ether sulfone) (PES) with different solvents including dimethyl sulfoxide (DMSO), N-methyl-2-pyrrolidone (NMP), and hexafluoroisopropanol (HFIP). The effect of solvent, carrier polymer (PES), and PANI content on formation of 3D conductive nanofibers with appropriate porosity were investigated. It was shown that a solvent with suitable properties should be selected in such a way that the composite nanofibers can be electrospun at the lowest concentration of PES. In this way, the ratio of PANI increased in the scaffold, the electrical conductivity of nanofibers enhanced, and the flat 2D structure of scaffold changed to a fluffy 3D structure. Among the three studied solvents, HFIP with the lowest boiling point and the lowest surface tension was the best solvent for the fabrication of PANI/PES nanofibers. PES could be electrospun at a concentration of 9% w/w in HFIP, while the optimum percentage of PES in DMSO and NMP was above 23% w/w to produce uniform nanofibers. 3D nanofibrous scaffold obtained from 0.5% PANI/9% PES/HFIP solution with electrical conductivity of 3.7 × 10–5 S/Cm and porosity of 92.81 ± 1.23%. Cell infiltration into the 3D nanofibers with low packing density improved compared to densely packed 2D nanofibers.

Flexible, Mechanically Robust, Solid-State Electrolyte Membrane with Conducting Oxide-Enhanced 3D Nanofiber Networks for Lithium Batteries.

Using a three-dimensional (3D) Li-ion conducting ceramic network, such as Li7La3Zr2O12 (LLZO) garnet-type oxide conductor, has proved to be a promising strategy to form continuous Li ion transfer paths in a polymer-based composite. However, the 3D network produced by brittle ceramic conductor nanofibers fails to provide sufficient mechanical adaptability. In this manuscript, we reported a new 3D ion-conducting network, which is synthesized from highly loaded LLZO nanoparticles reinforced conducting polymer nanofibers, by creating a lightweight continuous and interconnected LLZO-enhanced 3D network to outperform conducting heavy and brittle ceramic nanofibers to offer a new design principle of composite electrolyte membrane featuring all-round properties in mechanical robustness, structural flexibility, high ionic conductivity, lightweight, and high surface area. This composite-nanofiber design overcomes the issues of using ceramic-only nanoparticles, nanowires, or nanofibers in polymer composite electrolyte, and our work can be considered as a new generation of composite electrolyte membrane in composite electrolyte development.

Bioinspired modified graphite film with superb mechanical and thermoconductive properties

Lightweight, tough, heat-resistant and thermoconductive materials are being pursued to replace metals for the thermal management of high-power electrical devices in military, automotive and aerospace fields. In this work, interconnective poly(p-phenylene-2,6-benzobisoxazole) nanofiber network is used to modify graphite film by mimicking the unique multilayered microstructure of natural nacre. The coupling of well-oriented graphite nanosheet and robust 3D nanofiber network not only endows the modified graphite film with superb mechanical property, but also with excellent thermal conduction property. The tensile strength (200.5 ± 1.5 MPa), strain-to-failure (24.3 ± 0.6%), toughness (38.5 ± 0.7 MJ/m3) and folding endurance (>10,000 times) are 16.7-fold, 13.3-fold, 149.2-fold and 333.3-fold higher than those of pure graphite film without modification, respectively. The superb mechanical properties are integrated with excellent thermal properties, including high thermal conductivity (104.5–179.8 W/mK) and good thermal stability (652 °C), as well as nonflammability. Such integration of low density, superb mechanical and thermoconductive properties surpasses 7075-type aluminum alloy, a typical heat dissipation metal material used for thermal management in aerospace devices.

Minimally Invasive Delivery of 3D Shape Recoverable Constructs with Ordered Structures for Tissue Repair.

Minimally invasive procedures are becoming increasingly more common in surgery. However, the biomaterials capable of delivering biomimetic, three-dimensional (3D) functional tissues in a minimally invasive manner and exhibiting ordered structures after delivery are lacking. Herein, we reported the fabrication of gelatin methacryloyl (GelMA)-coated, 3D expanded nanofiber scaffolds, and their potential applications in minimally invasive delivery of 3D functional tissue constructs with ordered structures and clinically appropriate sizes (4 cm × 2 cm × 1.5 mm). GelMA-coated, expanded 3D nanofiber scaffolds produced by combining electrospinning, gas-foaming expansion, hydrogel coating, and cross-linking are extremely shape recoverable after release of compressive strain, displaying a superelastic property. Such scaffolds can be seeded with various types of cells, including dermal fibroblasts, bone marrow-derived mesenchymal stem cells, and human neural stem/precursor cells to form 3D complex tissue constructs. Importantly, the developed 3D tissue constructs can be compressed and loaded into a 4 mm diameter glass tube for minimally invasive delivery without compromising the cell viability. Taken together, the method developed in this study could hold great promise for transplantation of biomimetic, 3D functional tissue constructs with well-organized structures for tissue repair and regeneration using minimally invasive procedures like laparoscopy and thoracoscopy.

3D PCL/Gelatin/Genipin Nanofiber Sponge as Scaffold for Regenerative Medicine.

Recent advancements in tissue engineering and material science have radically improved in vitro culturing platforms to more accurately replicate human tissue. However, the transition to clinical relevance has been slow in part due to the lack of biologically compatible/relevant materials. In the present study, we marry the commonly used two-dimensional (2D) technique of electrospinning and a self-assembly process to construct easily reproducible, highly porous, three-dimensional (3D) nanofiber scaffolds for various tissue engineering applications. Specimens from biologically relevant polymers polycaprolactone (PCL) and gelatin were chemically cross-linked using the naturally occurring cross-linker genipin. Potential cytotoxic effects of the scaffolds were analyzed by culturing human dermal fibroblasts (HDF) up to 23 days. The 3D PCL/gelatin/genipin scaffolds produced here resemble the complex nanofibrous architecture found in naturally occurring extracellular matrix (ECM) and exhibit physiologically relevant mechanical properties as well as excellent cell cytocompatibility. Samples cross-linked with 0.5% genipin demonstrated the highest metabolic activity and proliferation rates for HDF. Scanning electron microscopy (SEM) images indicated excellent cell adhesion and the characteristic morphological features of fibroblasts in all tested samples. The three-dimensional (3D) PCL/gelatin/genipin scaffolds produced here show great potential for various 3D tissue-engineering applications such as ex vivo cell culturing platforms, wound healing, or tissue replacement.

Modifying polysulfide conversion and confinement by employing MoS2 nanofiber interlayer for high electrochemical performance

Owing to the high-energy density and specific capacity, lithium-sulfur batteries are regarded as the most promising energy storage systems, whereas the wide applications of lithium-sulfur batteries are hindered by the severe “shuttle effect” and sluggish redox kinetics of lithium polysulfides. Many strategies have been applied to modify the electrochemical performance, including preparing suitable sulfur hosts, designing functional interlayer, and protecting the lithium anode. Among these strategies, designing functional interlayers is considered as an efficient method to inhibit the shuttle effect and improve the redox kinetics of lithium polysulfides. Herein, MoS2 nanofiber (MSNF) interlayers are prepared and used in the lithium-sulfur batteries. On the one hand, the polysulfide migration could be inhibited due to the chemical adsorption between MoS2 and polysulfide. On the other hand, due to the 3D nanofiber structure of MoS2, it could provide the rapid electron/ion transport and fast redox kinetics. As a result, the lithium-sulfur batteries with MSNF interlayers display high specific capacity and excellent cycling stability.

The Effects of Tgfb1 and Csf3 on Chondrogenic Differentiation of iPS Cells in 2D and 3D Culture Environment.

Mesenchymal stem (MS) cells, embryonic stem (ES) cells, and induced pluripotent stem (iPS) cells are known for their ability to differentiate into different lineages, including chondrocytes in culture. However, the existing protocol for chondrocyte differentiation is time consuming and labor intensive. To improve and simplify the differentiation strategy, we have explored the effects of interactions between growth factors (transforming growth factor β1 (Tgfb1) and colony stimulating factor 3 (Csf3), and culture environments (2D monolayer and 3D nanofiber scaffold) on chondrogenic differentiation. For this, we have examined cell morphologies, proliferation rates, viability, and gene expression profiles, and characterized the cartilaginous matrix formed in the chondrogenic cultures under different treatment regimens. Our data show that 3D cultures support higher proliferation rate than the 2D cultures. Tgfb1 promotes cell proliferation and viability in both types of culture, whereas Csf3 shows positive effects only in 3D cultures. Interestingly, our results indicate that the combined treatments of Tgfb1 and Csf3 do not affect cell proliferation and viability. The expression of cartilaginous matrix in different treatment groups indicates the presence of chondrocytes. We found that, at the end of differentiation stage 1, pluripotent markers were downregulated, while the mesodermal marker was upregulated. However, the expression of chondrogenic markers (col2a1 and aggrecan) was upregulated only in the 3D cultures. Here, we report an efficient, scalable, and convenient protocol for chondrogenic differentiation of iPS cells, and our data suggest that a 3D culture environment, combined with tgfb1 and csf3 treatment, promotes the chondrogenic differentiation.

Nanofiber fluorescence coating for evaluation of complex solid-/gas-multi-phase and nano-/micro- multi-scale nanocomposite foam structure

Dual fluorescence design based on chemical coating and physical staining was successfully applied in revealing the multi-phase and multi-scale structure simultaneously in nanocomposite foams for the first time (that is nanometer-scale solid phase fillers selectively located chemical coating fluorescence, micrometer-scale solid phase cell walls selectively located physical staining fluorescence and air phase bubbles selectively located no fluorescence). The hybrid fluorescence modification method enables us to selectively locate different fluorescence dyes on nanofiber surface and in polymer matrix. Furthermore, quantitative analysis of the three-dimensional (3D) nanofiber network structure in nanocomposite foams is studied in-depth via numerical reconstruction. The evolution of 3D nanofiller network structure in multi-phase and multi-scale nanocomposite foams is demonstrated via both experimental visualization and numerical calculation. It is found that the average shortest nanofiber distance decreases with increasing cell wall thickness and with decreasing air content. Therefore, the dual fluorescence design exploring 3D functional filler network structure in complicated multi-phase and multi-scale nanocomposite foams greatly helps researchers study the structure-performance relationship of multi-functional materials with nanoscale functional fillers.

Large‐scale synthesis of compressible and re‐expandable three‐dimensional nanofiber matrices

AbstractDue to their biomimetic properties, electrospun nanofibers have shown great potential in many biomedical fields. However, traditionally‐produced nanofibers are typically two‐dimensional (2D) membranes limiting their applications. Herein, we report a large‐scale synthesis of compressible and re‐expandable three‐dimensional (3D) nanofiber matrices for potential biomedical applications. The reproducible mass production of such matrices is achieved using a multiple‐emitter electrospinning machine with a controlled environment (e.g., temperature, humidity, and air flow rate) followed by an innovative gas‐foaming expansion. The modified 20‐emitter circular array with 3D‐printed needle caps is capable of maintaining stable Taylor cones under extremely high flow rates. The introduction of such an emitter array allows for the production rate of 3D nanofiber matrices to increase by over 800 times while retaining the desired morphological, mechanical, and absorptive properties when compared to ones generated by a single‐nozzle electrospinning setup. Taken together, a feasible, optimized method has been demonstrated for scaling up production of shape‐recoverable, expansile nanofiber matrices, representing a step towards translating such materials into preclinical, large animal testing, clinical trials, and eventually clinical applications.