Event Abstract Back to Event Nanotechnology-assisted piezoelectrodes for cochlear stimulation Serena Danti1, Carlos Mota1, 2, Massimiliano Labardi3, Giada G. Genchi4, Dario Puppi2, Federica Chiellini2, Virgilio Mattoli4, Gianni Ciofani4, Luca Bruschini1 and Stefano Berrettini1 1 University of Pisa, Departmentof Surgical, Medical, Molecular Pathology and Emergency Medicine, Italy 2 University of Pisa, Department of Chemistry and Industrial Chemistry, Italy 3 National Council of Researches (CNR), Institute for Chemical-Physical Processes, Italy 4 Italian Institute of Technology (IIT), Center for Micro-BioRobotics @SSSA, Italy Introduction: Cochlear implants (CIs) are complex electronic devices which represent the only option to treat profound or severe sensorineural hearing loss. CIs show inherent drawbacks, like interference with magnetic fields and water-compatibility. It has been shown that piezoelectric materials alone can replace the function of cochlear sensory epithelium [1]. First studies used a silicon/polyvinylidene fluoride (PVDF) membrane, but the output voltage was still insufficient to excite the auditory neurons. This study is aimed at applying nanotechnology to achieve increased device sensitivity, via two strategies: (i) doping PVDF with piezoelectric nanoceramics to improve device performance, and (ii) tailoring architectural cues of materials to improve device efficiency at the neuron/material interface. Materials and Methods: Coaxial electrospinning (V = 20 kV, rotation velocity = 500-3.000 r.p.m) was used to obtain aligned ultrafine fibers from a PVDF solution (20% w/v dimethylformamide) mixed with barium titanate nanoparticles (BTNPs; composition range: 0%-20% w/w). Fiber diameter was measured via scanning electron microscopy (SEM) (n = 100). Fiber alignment was evaluated by 2D Fast Fourier Transform method. Presence and distribution of BTNPs in the fibers was observed with transmission electron microscopy (TEM). Piezoelectric crystallographic phases were assessed via X-ray diffraction. Piezoelectric properties were investigated with a set-up which measures the material deformation under an applied voltage. The substrates were cultured in vitro with PC12, as a model of neuronal cells, to assess cell viability (MTS assay) and differentiation (neurite sprout) under high frequency ultrasound (2 W, 5 s) for mechanic stimulation. Results and Discussion: Thin PVDF/BTNP fiber ribbons were produced. Fiber diameter varied depending on the rotation velocity and not on the BTNP concentration. Increasing rotation velocity gave rise to reduced fiber diameters and enhanced fiber alignment. Therefore, samples obtained at 3.000 r.p.m. were chosen for characterization (Figure 1). Piezoelectric crystal phases 2θ of PVDF and barium titanate were detected, and BTNPs resulted homogeneously dispersed in the fibers (Figure 1). In PVDF nanofiber ribbons, increasing BTNP concentration up to 20% (w/w) enhanced both direct and converse piezoelectric effects on a logarithmic scale (Figure 1), without exerting cytotoxic effects on PC12 cells (Figure 2). Neurite sprout was more pronounced on PVDF/BTNP 10% fibrous materials than in tissue culture plastics and averagely increased under ultrasound irradiation, with no statistical significance (Figure 2). Conclusion: Doping PVDF with nanoceramics can increase the piezoelectrode performance. Architectural features (e.g., aligned ultrafine fibers) can enhance the contact at the neuron/material interface. Obtaining highly sensitive piezoelectric CIs could substantially impact the quality of life of deaf people and reduce the healthcare costs. Italian Ministry of Health – Finalized Research Program 2011