3D Deformation Research Articles

Automated characterization and classification of 3D microstructures: an application to 3D deformation twin networks in titanium

The advent of techniques enabling three-dimensional (3D) analysis of objects, defects, and fields has been key to discoveries and paradigm shifts in molecular biology, astrophysics, medicine, quantum physics, etc. In materials science, the 3D nature of materials microstructures remains largely hidden; leading to a fragmented understanding of microstructure-property linkages. Current tools cannot characterize large volumes of 3D microstructures at fine resolution. To this end, this study introduces a graph-theory-based framework to automatically extract 3D microstructures and statistics of electron-backscatter diffraction datasets. Further, leveraging network science, the study introduces a new approach to classify and compare microstructures; the keystone to materials taxonomy. The significance of this tool is demonstrated by studying deformation twin structures in Titanium. The study reveals extraordinarily complex and tortuous twin networks never observed via traditional two-dimensional analysis. This changes our perception of the ability of metals to withstand severe microstructure changes without failing.

Effect of Peritalar Subluxation Correction for Progressive Collapsing Foot Deformity on Patient-Reported Outcomes.

Peritalar subluxation (PTS) is part of progressive collapsing foot deformity (PCFD). This study aimed to evaluate initial deformity correction and PTS optimization in PCFD patients with flexible hindfoot deformity undergoing hindfoot joint-sparing surgical procedures and its relationship with improvements in patient-reported outcome measures (PROMs) at latest follow-up. We hypothesized that significant deformity/PTS correction would be observed postoperatively, positively correlating with improved PROMs. A prospective comparative study was performed with 26 flexible PCFD patients undergoing hindfoot joint-sparing reconstructive procedures, mean age 47.1 years (range, 18-77). We assessed weightbearing computed tomography (WBCT) overall deformity (foot and ankle offset [FAO]) and PTS markers (distance and coverage maps) at 3 months, as well as PROMs at final follow-up. A multivariate regression model assessed the influence of initial deformity correction and PTS optimization in patient-reported outcomes. Mean follow-up was 19.9 months (6-39), and the average number of procedures performed was 4.8 (2-8). FAO improved from 9.4% (8.4-10.9) to 1.9% (1.1-3.6) postoperatively (P < .0001). Mean coverage improved by 69.6% (P = .012), 12.1% (P = .0343) and 5.2% (P = .0074) in, respectively, the anterior, middle, and posterior facets, whereas the sinus tarsi coverage decreased by an average 57.1% (P < .0001) postoperatively. Improvements in patient-reported outcomes were noted for all scores assessed (P < .03). The multivariate regression analysis demonstrated that improvement in both FAO and PTS measurements significantly influenced the assessed PROMs. This study demonstrated significant improvements in the overall 3D deformity, PTS markers, and PROMs following hindfoot joint-sparing surgical treatment in patients with flexible PCFD. More importantly, initial 3D deformity correction and improvement in subtalar joint coverage and extraarticular impingement have been shown to influence PROMs significantly and positively. Addressing these variables should be considered as goals when treating PCFD. Level II, prospective cohort study.

Hyperelastic constitutive model parameters identification using optical-based techniques and hybrid optimisation

AbstractIn this paper we propose a new optical-based technique to identify the constitutive relation coefficients of the hyperelastic material using a hybrid optimisation approach. This technique can be used in place of traditional mechanical testing of elastomers for applications that involve inhomogeneous deformation. The purpose of the proposed method is to identify the incompressible hyperelastic material constitutive relation coefficients using a single experiment under different loading cases. The method comprises sample surface 3D reconstruction and uses finite element simulations to replicate the experiments, and uses a hybrid optimisation technique to minimise the error between actual 3D deformations and FE simulation results. The proposed hybrid technique predicts the hyperelastic constitutive relation coefficients more accurately than other optimisation methods. This study introduces a novel approach by employing a subpixel image registration algorithm for 3D reconstruction. The method requires a single experiment with diverse loading cases to accurately determine the coefficients of hyperelastic constitutive relations. The setup is portable and can be accommodated in a small suitcase. For this purpose, an apparatus was constructed comprising a stereoscopic system with eight cameras and a six-degree-of-freedom force-torque sensor to measure the induced forces and torques during the experiments. We identified the constitutive relation coefficients of Ogden N1, Ogden N3, Yeoh, and Arruda-Boyce relations which are commonly used models for silicone materials, using a traditional uniaxial test, optical uniaxial test (experiments performed using a constructed optical system), and inhomogeneous deformations tests. The study demonstrated that the coefficients obtained from inhomogeneous deformation tests provided the most accurate FE predictions. It was also shown that hyperelastic constitutive relation coefficients obtained from traditional uniaxial tests are insufficient to describe the material behaviour when the material undergoes inhomogeneous deformations.

Intrinsically Stretchable Subthreshold Organic Transistors for Highly Sensitive Low‐Power Skin‐Like Active‐Matrix Temperature Sensors

AbstractStretchable wearable sensors ultimately require low power consumption and high response to physiological signals with skin conformability. However, power‐response tradeoff and strain‐dependent sensing instability remain key challenges for electronic skin (e‐skin) sensors. Herein,an intrinsically stretchable organic subthreshold transistor operating at low voltage (−1 V) is presented, leading to ultralow power consumption (&lt;1 nW) for highly sensitive skin‐like temperature sensory devices. The highly temperature‐dependent hopping transport of the fully stretchable subthreshold transistor exhibits high‐temperature sensitivity (9.4% °C−1) and excellent sensing stability up to 100% strain. A skin‐like subthreshold organic transistor active‐matrix array is successfully fabricated that sensed the surface temperature distribution under a 3D deformation.

Effects of 3D deformation and nonlinear stress–strain relationship on the Brazilian test for a transversely isotropic rock

To improve the accuracy of indirect tensile strength for a transversely isotropic rock in the Brazilian test, this study considered the three-dimensional (3D) deformation and the nonlinear stress–strain relationship. A parametric study of a numerical Brazilian test was performed for a general range of elastic constants, revealing that the 3D modeling evaluated the indirect tensile strength up to 40% higher than the plane stress modeling. For the actual Asan gneiss, the 3D model evaluated the indirect tensile strength up to 10% higher and slightly enhanced the accuracy of deformation estimation compared with the plane stress model. The nonlinearity in stress–strain curve of Asan gneiss under uniaxial compression was then considered, such that the evaluated indirect tensile strength was affected by up to 10% and its anisotropy agreed well with the physical intuition. The estimation of deformation was significantly enhanced. The further validation on the nonlinear model is expected as future research.

Skin deformation analysis for pre-operative planning of DIEAP flap reconstruction surgery.

Deep inferior epigastric artery perforator (DIEAP) flap reconstruction surgeries can potentially benefit from augmented reality (AR) in the context of surgery planning and outcomes improvement. Although three-dimensional (3D) models help visualize and map the perforators, the anchorage of the models to the patient's body during surgery does not consider eventual skin deformation from the moment of computed tomography angiography (CTA) data acquisition until the position of the patient while in surgery. In this work, we compared the 3D deformation registration from supine arms down (CTA position) to supine with arms at 90° degrees (surgical position), estimating the patient's skin deformation. We processed the data sets of 20 volunteers with a 3D rigid registration tool and performed a descriptive statistical analysis and statistical inference. With 2.45mm of root mean square and 2.89mm of standard deviation, results include 30% cases of deformation above 3mm and 15% above 4mm. Pose transformation deformation indicates that 3D surface data from the CTA scan position differs from data acquired in loco at the surgical table. Such results indicate that research should be conducted to construct accurate 3D models using CTA data to display on the patient, while considering projection errors when using AR technology.

Left Ventricular Myocardial Deformation Assessment by 4Dimensional XStrain Speckle Tracking Echocardiography: An Innovative Technology

Strain based imaging techniques, particularly speckle tracking echocardiography (STE) have been shown to have clinical utility in a variety of clinical settings. Currently, STE is being embraced and increasingly adopted in numerous echocardiographic laboratories worldwide. In context with contemporary non invasive cardiovascular procedures, 2Dimensional STE is most sought-after method to evaluate LV strain, rotation, twist and torsion. Two dimensional (2D), three dimensional (3D) and 4Dimensional (4D) deformation assessment by STE is inherently associated with certain limitations. For better appraisal of LV contractile properties recently introduced updated version of 4D XStrain STE has been employed to analyse the various complex multidimensional LV mechanics. This novel technology is a reliable, affordable and a simple tool for estimation of regional and global myocardial function. Furthermore, 4D XStrain STE can accurately quantify the 4D LV ejection-fraction, end-diastolic and end-systolic volumes and sphericity index (SI). However, this distinctive technology has not been widely adopted and its assessment is still limited to research applications. Hence we aimed to familiarize this innovative technology by comprehensively addressing its technique, advantages and limitations, with the intent that it may serve a more meaningful purpose in the current volatile scenario of cardio-vascular disease. Keywords: 4Dimensional XStrain Echocardiography, LV strain, LV rotation, LVtwist, LV untwist, LV torsion.

Full-field deformation and failure analysis for compression after impact of carbon fibre reinforced polymer laminates

Residual compressive strength after impact is a critical design factor in assessing damage tolerance of carbon fibre-reinforced polymers (CFRP) for structural applications in aircraft. This study investigated compression after impact (CAI) performance of quasi-isotropic CFRP laminates by measuring 3D deformation and strain using three-dimensional digital image correlation (3D DIC). Before the CAI, these test panels were subjected to drop-weight impact testing with energy levels representing visible impact damage (VID). During the CAI testing, deformation behaviours throughout the full loading sequence were captured by twin-camera imaging systems at the front and back faces of panels at standard speed. A single-camera high-speed imaging system captured ultimate failure behaviours at the sample front face. The single-camera high-speed system used four planar mirrors to achieve 3D DIC measurements. This study investigated the effect of boundary conditions on the compressive behaviours of the test panels, where the effect of additional support plates at the front and back surfaces to reduce the unsupported area was compared with the boundary condition of standard anti-buckling guides. The panels with added supports were found to experience deformations dominated by local buckling and final out-of-plane displacements in an S-shaped profile. These panels also exhibited residual strength reductions correlating to impact damage levels and ultimate failure initiating at the damaged region and propagating rapidly along the transverse axis. In comparison, the panels without added supports exhibited failure dominated by global buckling as manifested by large out-of-plane displacements, which had an arc-shaped profile. The DIC measurements were compared against those of mounted biaxial strain gauges. The single-camera high-speed system was shown not only to be feasible for high strain rate testing applications; it also offered a significant cost saving compared to an equivalent twin-camera high-speed system.

Free-HeadGAN: Neural Talking Head Synthesis With Explicit Gaze Control

We present Free-HeadGAN, a person-generic neural talking head synthesis system. We show that modeling faces with sparse 3D facial landmarks is sufficient for achieving state-of-the-art generative performance, without relying on strong statistical priors of the face, such as 3D Morphable Models. Apart from 3D pose and facial expressions, our method is capable of fully transferring the eye gaze, from a driving actor to a source identity. Our complete pipeline consists of three components: a canonical 3D key-point estimator that regresses 3D pose and expression-related deformations, a gaze estimation network and a generator that is built upon the architecture of HeadGAN. We further experiment with an extension of our generator to accommodate few-shot learning using an attention mechanism, in case multiple source images are available. Compared to recent methods for reenactment and motion transfer, our system achieves higher photo-realism combined with superior identity preservation, while offering explicit gaze control.

Monitoring artificial canals with multiple SAR satellites: A case study of the Changge Canal of the South-to-North Water Diversion Project in China

Surface monitoring of artificial canals is essential to ensure safe operation. Traditional geodetic tools are commonly employed to monitor the canal of China’s South-to-North Water Diversion Project (SNWDP), which is time-consuming and high-cost. The advanced satellite Synthetic Aperture Radar Interferometry (InSAR) is an effective tool to detect subtle surface deformation, while its application for monitoring canals is short of fine deformation interpretation on a structure scale. In this study, we applied multiple SAR satellites to monitor a typical canal of the Middle Route of the SNWD Project (MRP), the Changge Canal, by extracting the historical deformation, inverting two-dimensional (2D) deformation, and analyzing distortions. The result suggests that the focused canal section is experiencing continuous deformation caused by near coal mining activity. Its vertical and horizontal distortions both reach ±1.5 mm/100 m. The InSAR applicability, including visibility and sensitivity, is applied to the structure-level interpretation of deformation. The InSAR MPs distribution highly depends on canal structures' aspect and surface coverings. The canal structures with high coherence and lower R-Index (i.e., foreshortening) are favorable for detecting InSAR MPs; meanwhile, a few MPs are detected for the structures with high coherence but high R-index. For the sensitivity of the deformation vector along the downslope, its dependency on the aspect opposes InSAR MPs distribution. The structure-level interpretation considering InSAR applicability helps deploy targeted maintenance and analyze deformation mechanisms. The procedure of fine InSAR monitoring applies to similar water diversion projects.

A Lightweight Monocular 3D Face Reconstruction Method Based on Improved 3D Morphing Models.

In the past few years, 3D Morphing Model (3DMM)-based methods have achieved remarkable results in single-image 3D face reconstruction. However, high-fidelity 3D face texture generation has been successfully achieved with this method, which mostly uses the power of deep convolutional neural networks during the parameter fitting process, which leads to an increase in the number of network layers and computational burden of the network model and reduces the computational speed. Currently, existing methods increase computational speed by using lightweight networks for parameter fitting, but at the expense of reconstruction accuracy. In order to solve the above problems, we improved the 3D deformation model and proposed an efficient and lightweight network model: Mobile-FaceRNet. First, we combine depthwise separable convolution and multi-scale representation methods to fit the parameters of a 3D deformable model (3DMM); then, we introduce a residual attention module during network training to enhance the network's attention to important features, guaranteeing high-fidelity facial texture reconstruction quality; and, finally, a new perceptual loss function is designed to better address smoothness and image similarity for the smoothing constraints. Experimental results show that the method proposed in this paper can not only achieve high-precision reconstruction under the premise of lightweight, but it is also more robust to influences such as attitude and occlusion.

Three-Dimensional Shape and Deformation Measurements Based on Fringe Projection Profilometry and Fluorescent Digital Image Correlation via a 3 Charge Coupled Device Camera.

We propose a novel hybrid FPP-DIC technique to measure an object's shape and deformation in 3D simultaneously by using a single 3CCD color camera, which captures the blue fringe patterns and red fluorescent speckles within the same image. Firstly, red fluorescent speckles were painted on the surface of the specimen. Subsequently, 12 computer-generated blue fringe patterns with a black background were projected onto the surface of the specimen using a DLP projector. Finally, both the reference and deformed images with three different frequencies and four shifted phases were captured using a 3CCD camera. This technique employed a three-chip configuration in which red-green-blue chips were discretely integrated in the 3CCD color camera sensor, rendering independent capture of RGB information possible. Measurement of out-of-plane displacement was carried out through the implementation of Fringe Projection Profilometry (FPP), whereas the in-plane displacement was evaluated using a 2D Digital Image Correlation (DIC) method by leveraging a telecentric-lens-based optical system. In comparison to the traditional FPP-DIC hybrid methodology, the present approach showed a lower incidence of crosstalk between the fringe patterns and speckle patterns while also offering a corrective for the coupling of the in-plane displacement and out-of-plane displacement. Experimental results for the in-plane cantilever beam and out-of-plane disk comparisons with the traditional 3D-DIC method indicated that the maximum discrepancy obtained between FPP-DIC and 3D-DIC was 0.7 μm and 0.034 mm with different magnifications, respectively, validating the effectiveness and precision of the novel proposed FPP-DIC method.

The Value of Strain Echocardiography in Predicting Electrical Progression in Patients With Arrhythmogenic Right Ventricular Cardiomyopathy.

Arrhythmogenic right ventricular (RV) cardiomyopathy is a progressive disease characterized by the replacement of the normal myocardium with fibrofatty tissue. This study aimed to determine the value of echocardiographic RV deformation parameters in predicting electrical progression as assessed by serial changes in RV lead sensing and threshold in patients with arrhythmogenic RV cardiomyopathy. The present study recruited 40 patients with a definitive diagnosis of arrhythmogenic RV cardiomyopathy at a mean (SD) age of 38.6 (14.2) years between 2018 and 2020. All patients had received an implantable cardioverter-defibrillator for the primary or secondary prevention of sudden cardiac death. The patients underwent 2-dimensional (2D) and 3-dimensional (3D) transthoracic echocardiographic examinations and RV 2D and 3D strain analyses, comprising free-wall longitudinal strain, global longitudinal strain, and strain rate. They were then followed up for electrical progression. During a mean (SD) follow-up period of 20 (6) months, the RV lead amplitude decreased from 7.95 (IQR, 4.53-10.25) mV to 5.25 (IQR, 2.88-8.55) mV (P < .001), and the lead threshold increased from 0.75 (IQR, 0.50-0.79) V to 0.75 (IQR, 0.75-1.00) V (P < .001). Right ventricular 2D free-wall (ρ = 0.56, P = .01), RV 2D global (ρ = 0.58, P = .007), and RV 3D free-wall (ρ = 0.65; P = .003) longitudinal strain correlated with electrical progression. Right ventricular 2D and 3D deformation parameters were found to be significant predictors of electrical progression during follow-up of patients with arrhythmogenic RV cardiomyopathy. These findings suggest that echocardiography has a pivotal role in predicting patients at high risk for electrical progression.

Specific Solution of 3d Deformation Vector in Mine Subsidence: a Case Study of The Košice-Bankov Abandoned Magnesite Mine, Slovakia

Specific Solution of 3d Deformation Vector in Mine Subsidence: a Case Study of The Košice-Bankov Abandoned Magnesite Mine, Slovakia

Additive Manufacturing of Liquid Crystal Elastomer Actuators Based on Knitting Technology.

Liquid crystal elastomer (LCE) exhibits large and reversible deformability originating from the alignment of liquid crystal mesogens. Additive manufacturing provides high controllability in the alignment and shaping process of LCE actuators. However, it still remains a challenge to customize LCE actuators with both diverse 3D deformability and recyclability. In this study, a new strategy is developed to exploit knitting technique to additively manufacture LCE actuators. The obtained LCE actuators are fabric-structured with designed geometry and deformability. By accurately adjusting the parameters of the knitting patterns as modules, diverse geometry is pixel-wise designed, and complex 3D deformations including bending, twisting, and folding are quantitatively controlled. In addition, the fabric-structured LCE actuators can be threaded, stitched, and reknitted to achieve advanced geometry, integrated multi-functions and efficient recyclability. This approach allows the fabrication of versatile LCE actuators with potential applications in smart textiles and soft robots.

Growth friendly surgeries increase 3D true spine length at two-years post-operative.

Traditional coronal-plane vertical spine height measurements, such as T1-T12 and T1-S1 spine height, are influenced by 3-dimensional spinal deformity. Therefore, they are unreliable indicators of true spine growth. The novel 3D true spine length (3D-TSL) technique assesses spine growth regardless of the presence of 3D spinal deformity. This study evaluates the effect of growth friendly surgery on spine growth using the 3D-TSL technique at two years follow-up. Radiographs of early onset scoliosis (EOS) participants undergoing growth friendly surgery at a single institution were evaluated at pre-index, post-index, and at 2-year follow-up. Scoliosis, kyphosis, traditional coronal height and 3D-TSL were measured. Twenty-four EOS patients (14 female) with a mean age of 6.3years were included. Scoliosis improved from 77.8 ± 18.7° pre-index to 56.0 ± 17.8° post-index (p < 0.001); but increased at 2years to 65.9 ± 20.1° (p < 0.05). Kyphosis improved from 56.0 ± 20.7° pre-index to 39.3 ± 13.8° post-index (p < 0.001); and increased again at 2years to 48.6 ± 16.4° (p < 0.05). Traditional T1-S1 height increased from pre-index to post-index, to 2-year follow-up (245.5 ± 37.2mm to 275.8 ± 42.7mm; p < 0.001, to 288.0 ± 41.7mm; p = 0.005). As expected, T1-S1 3D-TSL did not change from pre to immediately post-index (305.6 ± 43.9mm vs 306.5 ± 44.0mm); there was a significant change from post-index to 2-year follow-up (334.1 ± 48.0mm; p < 0.001). Out of plane changes in scoliosis and kyphosis over time justify the use of the 3D-TSL for this cohort of patients. There was no significant change in 3D-TSL from pre-index to post-operatively; however, during the growth phase of treatment 3D-TSL increased significantly by 27.6mm.

Defining the Borderline Dysplastic Hip: High Variability in Acetabular Coverage and Femoral Morphology on Low-Dose Computed Tomography.

Borderline acetabular dysplasia is commonly radiographically defined as a lateral center-edge angle (LCEA) of 20° to 25°. While the variability of plain radiographic assessment of this population has been reported, an understanding of the variability of 3-dimensional (3D) hip morphology remains to be better defined. To investigate the variability of 3D hip morphology present on low-dose computed tomography (CT) in the setting of symptomatic borderline acetabular dysplasia and to determine if plain radiographic parameters correlate with 3D coverage. Cohort study (diagnosis); Level of evidence, 2. A total of 70 consecutive hips with borderline acetabular dysplasia undergoing hip preservation surgery were included in the current study. Plain radiographic evaluation included LCEA, acetabular inclination, anterior center-edge angle (ACEA), anterior wall index (AWI), posterior wall index (PWI), and alpha angles on anteroposterior, 45° Dunn, and frog-leg views. All patients underwent low-dose pelvic CT for preoperative planning, which allowed detailed characterization of 3D morphology relative to normative data. Acetabular morphology was assessed with radial acetabular coverage (RAC) calculated according to standardized clockface positions from 8:00 (posterior) to 4:00 (anterior). Coverages at 10:00, 12:00, and 2:00 were classified as normal, undercoverage, or overcoverage relative to 1 SD from the mean of normative RAC values. Femoral morphology was assessed with femoral version, alpha angle (measured at 1:00 increments), and maximum alpha angle. Correlation was assessed with the Pearson correlation coefficient (r). Lateral coverage (12:00 RAC) was deficient in 74.1% of hips with borderline dysplasia. Anterior coverage (2:00 RAC) was highly variable, with 17.1% undercoverage, 72.9% normal, and 10.0% overcoverage. Posterior coverage (10:00 RAC) was also highly variable, with 30.0% undercoverage, 62.9% normal, and 7.1% overcoverage. The 3 most common patterns of coverage were isolated lateral undercoverage (31.4%), normal coverage (18.6%), and combined lateral and posterior undercoverage (17.1%). The mean femoral version was 19.7°± 10.6° (range, -4° to 59°), with 47.1% of hips having increased femoral version (>20°). The mean maximum alpha angle was 57.2° (range, 43°-81°), with 48.6% of hips having an alpha angle ≥ 55°. The ACEA and AWI were poorly correlated with radial anterior coverage (r = 0.059 and 0.311, respectively), while the PWI was strongly correlated with radial posterior coverage (r = 0.774). Patients with borderline acetabular dysplasia demonstrate highly variable 3D deformities, including anterior, lateral, and posterior acetabular coverage; femoral version; and alpha angle. Plain radiographic assessments of anterior coverage are poorly correlated with anterior 3D coverage on low-dose CT.

Phase-transforming mechanical metamaterials with dynamically controllable shape-locking performance.

Active mechanical metamaterials with customizable structures and deformations, active reversible deformation, dynamically controllable shape-locking performance and stretchability are highly suitable for applications in soft robotics and flexible electronics, yet it is challenging to integrate them due to their mutual conflicts. Here, we introduce a class of phase-transforming mechanical metamaterials (PMMs) that integrate the above properties. Periodically arranging basic actuating units according to the designed pattern configuration and positional relationship, PMMs can customize complex and diverse structures and deformations. Liquid-vapor phase transformation provides active reversible large deformation while a silicone matrix offers stretchability. The contained carbonyl iron powder endows PMMs with dynamically controllable shape-locking performance, thereby achieving magnetically assisted shape locking and energy storing in different working modes. We build a theoretical model and finite element simulation to guide the design process of PMMs, so as to develop a variety of PMMs with different functions suitable for different applications, such as a programmed PMM, reconfigurable antenna, soft lens, soft mechanical memory, biomimetic hand, biomimetic flytrap and self-contained soft gripper. PMMs are applicable to achieve various 2D deformations and 2D-to-3D deformations, and integrate multiple properties, including customizable structures and deformations, active reversible deformation, rapid reversible shape locking, adjustable energy storing and stretchability, which could open a new application avenue in soft robotics and flexible electronics.

Agarose-based 3D Cell Confinement Assay to Study Nuclear Mechanobiology.

Cells in living tissues are exposed to substantial mechanical forces and constraints imposed by neighboring cells, the extracellular matrix, and external factors. Mechanical forces and physical confinement can drive various cellular responses, including changes in gene expression, cell growth, differentiation, and migration, all of which have important implications in physiological and pathological processes, such as immune cell migration or cancer metastasis. Previous studies have shown that nuclear deformation induced by 3D confinement promotes cell contractility but can also cause DNA damage and changes in chromatin organization, thereby motivating further studies in nuclear mechanobiology. In this protocol, we present a custom-developed, easy-to-use, robust, and low-cost approach to induce precisely defined physical confinement on cells using agarose pads with micropillars and externally applied weights. We validated the device by confirming nuclear deformation, changes in nuclear area, and cell viability after confinement. The device is suitable for short- and long-term confinement studies and compatible with imaging of both live and fixed samples, thus presenting a versatile approach to studying the impact of 3D cell confinement and nuclear deformation on cellular function. This article contains detailed protocols for the fabrication and use of the confinement device, including live cell imaging and labeling of fixed cells for subsequent analysis. These protocols can be amended for specific applications. © 2023 Wiley Periodicals LLC. Basic Protocol 1: Design and fabrication of the confinement device wafer Basic Protocol 2: Cell confinement assay Support Protocol 1: Fixation and staining of cells after confinement Support protocol 2: Live/dead staining of cells during confinement.

Displacement field reconstruction in landslide physical modeling by using a terrain laser scanner – Part 1: Methodology, error analysis and validation

Laser scanning technology has been widely used in landslide aspects. However, the existing deformation analysis based on terrain laser scanners can only provide limited information, which is insufficient for understanding landslide kinematics and failure mechanisms. To overcome this limitation, this paper proposes an automated method for processing point clouds collected in landslide physical modeling. This method allows the acquisition of quantitative three-dimensional (3D) deformation field information. The results show the organized and spatially related point cloud segmentation in terms of spherical targets. The segmented point clouds can be fitted to determine the locations of all preset targets and their corresponding location changes. The proposed method has been validated based on theoretical analysis and numerical and physical tests, which indicates that it can batch-process massive data sets with high computational efficiency and good noise resistance. Compared to existing methods, this method shows a significant potential for understanding landslide kinematics and failure mechanisms and advancing the application of 3D laser scanning in geotechnical modeling.