Large bioprinted tissues (dm3) are more and more accessible but their in vitro culture and maturation conditions stay an unchartered territory. In the present report, we aim to present a preliminary study of endothelialized large bioprinted tissues (fibroblast and human dermal microvascular endothelial cells) maturation using silicone 3D printed perfusion system (bioreactor). Computational Fluid Dynamics (CFD) simulation was used to relate the theoretical culture medium flow path within the large bioprinted tissue with the actual tissue morphology and composition, obtained through histological observations. The obtained results demonstrate the positive impact of using dynamic maturation conditions (300 mL/h culture medium flow rate) on the extracellular matrix production and the conservation of the large bioprinted tissue internal geometry. Clear differences between static and dynamic culture conditions were herein found. Finally, typical microvascular organization, composed of human dermal microvascular endothelial cells organized around an open lumen, were found within the large bioprinted tissue.