3D Analysis Research Articles

Analytical model for flexural-torsional coupling behavior of prestressed corrugated steel-concrete composite girders

Prestressed corrugated steel-concrete (PCSC) composite girders are often subjected to combined bending and torsion in practice, but the existing theoretical models have mainly focused on pure torsional behavior. Thus, this paper proposed a modified combined action softened truss model (MCA-STM) to predict the flexural-torsional coupling behavior and failure modes of PCSC composite girders. The equivalent loading and nonlinear equations were modified, and the convergence criteria of flexural and torsional failure were established, considering the structural characteristics of PCSC composite girders. A new optimized algorithm was employed to solve the MCA-STM, providing a more efficient and numerically stable solution procedure. A 3D finite element analysis (FEA) model of the PCSC composite girder was established, which was verified by comparing the available test results. A parametric study was carried out based on the verified FEA model to get more datasets with various parameters, and the results were compared to the proposed analytical model. The curves obtained by the FEA and MCA-STM are in good agreement, indicating that the proposed analytical model can predict the full flexural-torsional coupling behavior and failure modes of the PCSC composite girders.

2D and 3D microstructural analysis of the iliotibial tract.

The fascial system has gained recognition for its integral role in connecting skin, superficial and deep fasciae, and underlying muscles. However, consensus on its microstructure depending on its topography remains elusive as well as its implications in clinical practices, such as reconstructive surgery and physiotherapy techniques. This study focuses on the iliotibial tract (ITT) implicated in the iliotibial band syndrome. The goal is to describe microstructural characteristics using classical 2D histology and cryogenic contrast-enhanced microcomputed tomography (cryo-CECT) such as the total thickness, number of layers, layer thickness, fibre orientation and tortuosity, according to the specific topography. The total thickness of the ITT varied across topographic regions, with the superior part being on average thicker but non-significantly different from the other regions. The inferior part showed heterogeneity, with the anterior region (AI) being the thinnest and the posterior one (PI) the thickest. The ITT exhibited 1-3 layers, with no significant differences among regions. Most commonly, it consisted of two layers, except for the antero-superior (AS) and antero-middle (AM) regions, which sometimes had only one layer. The posterior regions frequently had 2 or 3 layers, with the PI region having the highest mean (2.7 layers). The intermediate layer was the thickest one, varying from the AI region (0.368 mm ± 0.114) to the PI region (0.640 mm ± 0.305). The superficial layer showed regional variability, with the AS region being the thinnest. The deep layer appeared thinner than the superficial one. Fibre orientation analysis indicated that the intermediate layer mainly consisted of oblique longitudinal fibres, orientated downward and forward, while the superficial and deep layers had transversal or oblique transversal fibres. Cryo-CECT 3D observations confirmed these findings, revealing distinct orientations for different layers. Fibre tortuosity exhibited differences based on orientation. Transversal fibres (>65°) were significantly less tortuous than longitudinal fibres (<25°) and oblique intermediate fibres (25°-65°), aligning with 3D plot observations. This quantitative study highlights various microstructural characteristics of the ITT, offering insights into its regional variations. The analysis accuracy is increased due to the novel technology of cryo-CECT which emerges as a valuable tool for precise assessment of 3D fibre orientation and tortuosity. These findings contribute to a deeper understanding of the ITT structure, useful in clinical practices, such as reconstructive surgery and physiotherapy, and future research endeavours.

Integrating Vascular Phenotypic and Proteomic Analysis in an Open Microfluidic Platform.

This research introduces a vascular phenotypic and proteomic analysis (VPT) platform designed to perform high-throughput experiments on vascular development. The VPT platform utilizes an open-channel configuration that facilitates angiogenesis by precise alignment of endothelial cells, allowing for a 3D morphological examination and protein analysis. We study the effects of antiangiogenic agents─bevacizumab, ramucirumab, cabozantinib, regorafenib, wortmannin, chloroquine, and paclitaxel─on cytoskeletal integrity and angiogenic sprouting, observing an approximately 50% reduction in sprouting at higher drug concentrations. Precise LC-MS/MS analyses reveal global protein expression changes in response to four of these drugs, providing insights into the signaling pathways related to the cell cycle, cytoskeleton, cellular senescence, and angiogenesis. Our findings emphasize the intricate relationship between cytoskeletal alterations and angiogenic responses, underlining the significance of integrating morphological and proteomic data for a comprehensive understanding of angiogenesis. The VPT platform not only advances our understanding of drug impacts on vascular biology but also offers a versatile tool for analyzing proteome and morphological features across various models beyond blood vessels.

3D finite element analysis of carbon fiber reinforced aluminium alloy wheel

3D finite element analysis of carbon fiber reinforced aluminium alloy wheel

Synergistic antimicrobial activities of aqueous extract derived from olive byproduct and their modes of action

BackgroundPlant-derived antimicrobials (PDAs) are considered a viable alternative to synthetic antimicrobial agents. Diverse antimicrobial mechanisms of PDAs significantly reduce the risk of developing antimicrobial resistance. Utilization of PDAs also offers economic and environmental advantages, as they can be derived from agricultural byproducts, such as olive pomace.ResultsIn this study, a green, water-based, ultrasound-assisted extraction (UAE) was deployed to obtain aqueous olive pomace extract (OPE) from dry olive pomace. Total phenolic content, extraction yield, chemical compositions, and antimicrobial activities of OPE were evaluated. In addition, the potential synergistic interaction between the phenolic components in OPE and the antimicrobial mechanisms underlying the synergistic interaction were characterized. The results show that ca. 25 mg GAE/g of extraction yields were achieved by the UAE of dry olive pomace. Based on the high-performance liquid chromatography (HPLC) analysis, diverse phenolic compounds such as gallic acid (GA), hydroxytyrosol (HT), and 4-hydroxyphenylacetic acid (4-HPA) were identified in OPE. OPE exhibited strong antimicrobial activities, and 0.2 mg GAE/mL of OPE achieved > 5 log reductions of Escherichia coli O157:H7 and Listeria innocua cells within 30 min of treatment. A 3D isobologram analysis demonstrated that OPE exhibited strong synergistic antimicrobial activities, compared to those of individual phenolic components (GA, HT, or 4-HPA), showing interaction index (γ) of 0.092 and 0.014 against E. coli O157:H7 and L. innocua, respectively (γ < 1: synergistic activity). Antimicrobial mechanism analyses revealed that phenolic components in OPE exerted strong synergistic activities through diverse modes of action, and increased levels of oxidative stress, membrane damage, and decreased levels of metabolic activities were observed in the OPE-treated bacterial cells.ConclusionsThese findings demonstrate an approach for valorizing agricultural byproducts to develop plant byproduct-based antimicrobials with strong synergistic activities. Multiple modes of action of this byproduct extract may enable the control of diverse microbes in food and agriculture systems.Graphical abstract

Retrospective analysis of ideal needle puncture angles and depths for temporomandibular joint arthrocentesis using CBCT data

BackgroundThis study aimed to investigate the range of angles and depths necessary for effective entry into the TMJ using CBCT images, focusing on classical Holmlund Hellsing points and a two-needle approach.MethodsA retrospective cohort of CBCT images from January 2020 to November 2023 was analysed using 3D analysis to determine the variance in the required angles and depths.ResultsThe average age of the 68 participants included in the study was 29.5 ± 11.1, 58.8% of the participants were female and 41.2% were male. The anterior needle measurements showed a relatively low standard deviation(SD) in depth(SD:3.6) with a low variance coefficient(12.5%), whereas the axial and coronal angles exhibited greater variability(SD:9.1 and 7.6, respectively). For the posterior needles, moderate SDs in depth(SD:3.5) and greater variabilities in axial and coronal angles(SD:9.6 and 5.3, respectively) were observed. A weak negative correlation was observed between the axial angle of the posterior needle and age(p: 0.028, Pearson r: -0.29) Anterior needle depth (p:0.037) and posterior needle axial angle(p:0.014) were greater in males than females. The anterior needle depth in patients with temporamandibular disease was greater than in those without(p:0,03).ConclusionThere were significant differences in the angle measurements for both anterior and posterior needles, but lower variance in depth. The depths and angles of the needles did not correlate with age.

Minor Humulane and Eremophilane Sesquiterpenoids from Syringa pinnatifolia and Their Protective Effect Against Oxidative Injuries in H9c2 Cells.

As a part of systematic research, an ongoing phytochemical investigation of the sesquiterpenoid-containing fraction led to the isolation of five new sesquiterpenoids from the peeled stems of Syringa pinnatifolia, including two pairs of enantiomeric humulane-type (±)-alashanoids A1 and B1 (1 and 2) and one eremophilane-type alashanoid C1 (3). These structures were elucidated by the analysis of extensive spectroscopic data, including ESI-MS and 1D and 2D NMR, and the absolute configuration was determined by comparing its experimental and calculated electronic circular dichroism and calculated NMR. These isolates exhibited moderate in vitro cardioprotective effects against oxidative injuries in H9c2 cells.

Abietane diterpenoid salvipisone: Structure elucidation, conformational analysis, and antimicrobial activity

Salvipisone, an abietane diterpenoid, was isolated from the roots of Salvia aethiopis L. using a combination of chromatographic separation techniques. Its structure was elucidated employing a variety of spectroscopic methods, including ultraviolet (UV), infrared (IR), 1D and 2D nuclear magnetic resonance (NMR), and mass spectrometry. A comprehensive analysis of experimental 1D and 2D NMR data, incorporating 1H iterative full spin analysis and higher-order multiplet simulation, revealed discrepancies with previously reported NMR data for this compound. Detailed examination of the 1H NMR spectrum's spin-spin coupling structure provided insights into the relative populations of the three staggered conformers around the C12–C13 bond at room temperature. The conformer populations were determined using the relationship between the experimental values for vicinal coupling constants and the estimated ones for the preferred staggered conformers, yielding a ratio of approx. 80:4:16 for anti, gauche, and gauche′-conformers, respectively. Possible reasons for the observed unevenly populated staggered conformers were also discussed. The antimicrobial activity of salvipisone was evaluated using in vitro and in silico methods. In vitro antimicrobial assays demonstrated that salvipisone exhibited the highest inhibitory effect against Staphylococcus aureus ATCC and Enterococcus faecalis ATCC (MIC = 3.125 μg/mL), followed by Bacillus subtilis ATCC (MIC = 6.25 μg/mL). In contrast, concentrations above 100 μg/mL were necessary to inhibit Escherichia coli ATCC and Klebsiella pneumoniae ATCC. In silico studies suggested that salvipisone, particularly its keto form, has significant potential for inhibiting the enzyme gyrase, highlighting its mechanism of antimicrobial action. Our findings highlight the promising antimicrobial and inhibitory properties of salvipisone, particularly against Gram-positive bacteria such as S. aureus.

Semi-automated vertex placement for lattice radiotherapy and dosimetric verification using 3D polymer gel dosimetry.

To evaluate the feasibility of an open-source, semi-automated, and reproducible vertex placement tool to improve the efficiency of lattice radiotherapy (LRT) planning. We used polymer gel dosimetry with a Cone Beam CT (CBCT) readout to commission this LRT technique. We generated a volumetric modulated arc therapy (VMAT)-based LRT plan on a 2 L NIPAM polymer gel dosimeter using our Eclipse Acuros version 15.6 AcurosXB beam model, and also recalculated the plan with a pre-clinical Acuros v18.0 dose calculation algorithm with the enhanced leaf modelling (ELM). With the assistance of the MAAS-SFRThelper software, a lattice vertex diameter of 1.5cm and center-to-center spacing of 3cm were used to place the spheres in a hexagonal, closed packed structure. The verification plan included four gantry arcs with 15°, 345°, 75°, 105° collimator angles. The spheres were prescribed 20Gy to 50% of their combined volume. The 6 MV Flattening Filter Free beam energy was used to deliver the verification plan. The dosimetric accuracy of the LRT delivery was evaluated with 1D dose profiles, 2D isodose maps, and a 3D global gamma analysis. Qualitative comparisons between the 1D dose profiles of the Eclipse plan and measured gel showed good consistency at the prescription dose mark. The average diameter measured 13.3±0.2mm (gel for v15.6), 12.6mm (v15.6 plan), 13.1±0.2mm (gel for v18.0), and 12.3mm (v18.0 plan). 3D gamma analysis showed that all gamma pass percent were>95% except at 1% and 2% at the 1mm distance to agreement criteria. This study presents a novel application of gel dosimetry in verifying the dosimetric accuracy of LRT, achieving excellent 3D gamma results. The treatment planning was facilitated by publicly available software that automatically placed the vertices for consistency and efficiency.

Examination of Left-Right Differences and Intertrial Variability in Grand Jeté Among Professional Ballet Dancers.

Introduction: Ballet demands diverse physical prowess, requiring dancers to execute movements symmetrically, irrespective of their dominant leg. Ballet often includes jumps, including the basic open-leg leap-the grand jeté-which requires uniform performance regardless of the leg on which the leap is initiated. However, no studies have simultaneously evaluated the effects of dominant leg or movement variation on jump height, leg split angle, jump time, and floor reaction forces during take-off and landing, which are related to the feeling of floating in the grand jeté. This study aimed to determine whether the high-level, stable, and beautiful performance required of professional ballet dancers in the grand jeté is affected by the dominant leg. Methods: Twelve female ballet dancers, all right leg dominant, performed the grand jeté 3 times on each side, distinguishing between dominant (right leg landing) and non-dominant (left leg landing) grand jetés. Utilising 3D movement analysis, we measured jump height, jump time, maximum leg split angle, and maximum vertical ground reaction force (VGRF) during take-off and landing. Mean values and coefficients of variation were calculated for each analysed parameter. Paired sample t-tests were conducted to assess differences between left and right grand jetés, with a significance level set at P < .05. Results: Statistically significant differences were observed in jump height (P = .028) and jump time (P = .001) when comparing the average of three trials for each side. However, no significant differences were found in maximum leg split angle (P = 0.643), maximum VGRF at take-off (P = .200), and maximum VGRF at landing (P = .109). In addition, no significant differences in coefficients of variation were identified for all items. Conclusion: Ballet dancers showed consistent performance on dominant and non-dominant legs but higher and longer jumps for grand jetés landing on the dominant leg, which may have affected overall performance.

Relation between stretch and activation of the medial gastrocnemius muscle during gait in children with cerebral palsy compared to typically developing children

Stretch hyperreflexia is often a target for treatment to improve gait in children with spastic cerebral palsy (CP). However, the presence of stretch hyperreflexia during gait remains debated. Therefore, we assessed the relation between gastrocnemius medialis muscle-tendon stretch and muscle activation during gait in children with CP compared to typically developing (TD) children. 3D gait analysis including electromyography (EMG) and dynamic ultrasound was carried out to assess, respectively gastrocnemius medialis activation and fascicle, belly, and tendon stretch during treadmill walking. Musculotendon-unit stretch was also estimated using OpenSim. Ratios of EMG/peak lengthening velocities and accelerations were compared between CP and TD. Velocity and acceleration peaks prior to EMG peaks were qualitatively assessed. EMG/velocity and EMG/acceleration ratios were up to 500% higher for CP (n = 14) than TD (n = 15) for most structures. Increased late swing muscle activation in CP was often preceded by fascicle and musculotendon-unit peak lengthening velocity, and early stance muscle activation by peaks in multiple structures. Increased muscle activation in CP is associated with muscle-tendon stretch during gait. Concluding, late swing muscle activation in CP appears velocity-dependent, whereas early stance activation can be velocity- and acceleration-dependent. These insights into stretch reflex mechanisms during gait can assist development of targeted interventions.

Targeted isolation of dimeric sesquiterpene lactones and sesquiterpene derivatives from the roots of Inula helenium

LC-HRMS/MS-based molecular networking was applied to investigate the bioactive sesquiterpene lactones and their analogs contained in Inula helenium, enabling the isolation of four undescribed eudesmane-eudesmane sesquiterpene dimers (1–4), a sesquiterpene-amino acid adduct (5), and 17 known sesquiterpenes (6–22). The structures were determined based on 1D, 2D NMR, and HRESIMS data analysis, as well as DP4+ probability analyses and ECD calculations. The biosynthetic pathway of the sesquiterpene dimers is postulated to proceed via the Diels-Alder reaction as the key step. The inhibitory activity of all isolates on LPS-induced nitric oxide (NO) production in RAW 264.7 macrophages was evaluated. Compounds 4, 17, and 20 exhibited significant inhibitory activity against NO production, with IC50 values of 8.4, 5.5, and 9.1 μM, respectively.

3D in situ observation of the capillary infiltration of molten silicon in a SiC/SiC composite by X-ray tomography

The Liquid Silicon Infiltration (LSI) process is used to decrease residual porosity of SiC/SiC composite materials. However, it is not fully mastered since the mechanisms involved at 1500 °C under high vacuum are complex to analyze, especially without direct observation. Previous work had demonstrated the feasibility of using X-ray radiography to observe the front rise of silicon in a SiC/SiC composite material during the LSI process. 2D observations, as a first approach, could give a basic understanding of the mechanisms but raised several interrogations due to the superposition of these phenomena along the thickness preventing any quantification. The setup has been improved in order to make X-ray tomography using a fully integrated DC motor in place of the rotation stage commonly used. The sets of X-ray tomographs confirm two successive fillings. First, the molten silicon rapidly and non uniformly invades the accessible intergranular micro porosity of the powder. Then, the liquid slowly fills the remaining isolated powder areas. Once the SiC matrix is fully saturated, the liquid fills the bigger porosities such as cracks and intra yarn macro porosities. In addition, this 3D analysis enabled to give a better comprehension of the non uniform wetting front in the SiC matrix powder. During the 1st step, the accessibility of the powder was known to have a major effect on the speed of progression. Also, the cracks network plays a key role in the filling of the isolated areas in the powder matrix.

Mandibular shape and diet in extant primates: a 3D geometric morphometric analysis.

Establishing a link between mandibular morphology and diet in extant primates has long been a goal in biological anthropology because it should provide important insight into the diets of extinct primates, including fossil hominins. To date, efforts to explore this question have produced mixed results, largely perhaps due to a reliance on the use of 2D morphological data. Here, we report a study where we investigated whether 3D shape data would provide a clearer picture. We used geometric morphometrics to analyse 3D mandibular shape variation in a sample of > 200 primate specimens, representing individuals from 27 species and five families. Two sets of analyses investigated i) whether there was a relationship between mandibular shape and four standard dietary categories and ii) whether there was a relationship between mandibular shape and a well-known index of diet quality. We found an association between mandibular shape and the dietary categories when we employed raw Procrustes coordinates and allometry-free residuals, but the relationship was weak to non-existent when the effects of phylogeny were taken into account. We found no relationship between shape and the diet quality index, no matter whether the data were raw, corrected for the effects of allometry, corrected for the effects of phylogeny, or corrected for the effects of both allometry and phylogeny. Taken together, the results of the two sets of analyses suggest that there is a weak relationship between 3D mandibular shape and diet in extant primates. Allometry and phylogeny appear to be more important influences on the 3D shape of extant primate mandibles than is diet. We conclude from this that 3D analysis of mandibular shape is unlikely to further illuminate the diets of extinct primates, and research efforts should, therefore, be directed elsewhere.

Research on improving the flexural performance of alkali-activated geopolymer cemented coal gangue through layered addition of fibers

The brittle characteristics of geopolymers during failure seriously hinder their practical engineering applications. To improve the toughness of low-energy alkali-activated geopolymer cementing coal gangue (AGCCG), the flexural performance of alkali-activated geopolymer cementing coal gangue (AGCCG) was enhanced by layering polypropylene fibers. Based on DIC digital speckle technology, the four-point flexure test of AGCCG was carried out, and the strength formation mechanism of the material was analyzed in depth by combining SEM-EDS and XRD. 3D visualization and analysis of the inside of the specimen with the help of CT scanning technology. The internal crack evolution law of the sample was illustrated by PFC numerical simulation. The results showed that the crack formation threshold, peak flexural strength and residual flexural strength of the specimens reached a local maximum at 3 % NaOH doping. With the increase of NaOH dosage, the cementitious product becomes more and more dense and homogeneous, and the area of void defects in the hydration product within the field of view becomes smaller and smaller. The peak bending strength of specimen B4-#4 is 79.43 % of that of specimen B5-#1–4, in which the cost of specimen B5-#1–4 is four times of that of specimen B4-#4, and the way of fiber assignment in specimen B4-#4 is more reasonable when considered together. The layered addition of fibers has a directional strengthening effect on the mechanical properties of geopolymer, and the overall addition of fibers can improve the overall strength and toughness of geopolymer. The numerical simulation results basically coincide with the macroscopic test results, and are consistent with the progressive damage law of the specimens obtained from the numerical scattering test. The results of the study can provide a theoretical basis for improving the bearing capacity of geopolymer and optimizing its structural performance. As well as reducing the impact of solid waste on the environment and improving the efficiency of resource use.

Chronic undernutrition impairs renal mitochondrial respiration accompanied by intense ultrastructural damage in juvenile rats

This study investigated whether chronic undernutrition alters the mitochondrial structure and function in renal proximal tubule cells, thus impairing fluid transport and homeostasis. We previously showed that chronic undernutrition downregulates the renal proximal tubules (Na++K+)ATPase, the main molecular machine responsible for fluid transport and ATP consumption. Male rats received a multifactorial deficient diet, the so-called Regional Basic Diet (RBD), mimicking those used in impoverished regions worldwide, from weaning to a juvenile age (3 months). The diet has a low content (8 %) of poor-quality proteins, low lipids, and no vitamins compared to control (CTR). We investigated citrate synthase activity, mitochondrial respiration (oxygraphy) in phosphorylating and non-phosphorylating conditions with different substrates/inhibitors, potential across the internal membrane (Δψ), and anion superoxide/H2O2 formation. The data were correlated with ultrastructural alterations evaluated using transmission electron microscopy (TEM) and focused ion beam scanning electron microscopy (FIB-SEM). Citrate synthase activity decreased (∼50 %) in RBD rats, accompanied by a similar reduction in respiration in non-phosphorylating conditions, maximum respiratory capacity, and ATP synthesis. The Δψ generation and its dissipation after carbonyl cyanide-4-(trifluoromethoxy) phenylhydrazone remained unmodified in the survival mitochondria. H2O2 production increased (∼100 %) after Complex II energization. TEM demonstrated intense matrix vacuolization and disruption of cristae junctions in a subpopulation of RBD mitochondria, which was also demonstrated in the 3D analysis of FIB-SEM tomography. In conclusion, chronic undernutrition impairs mitochondrial functions in renal proximal tubules, with profound alterations in the matrix and internal membrane ultrastructure that culminate with the compromise of ATP supply for transport processes.

Challenges and possibilities of the manual metallographic serial sectioning process using the example of a quantitative microstructural analysis of graphite in cast iron

Abstract The 3D microstructure analysis presented in this study focuses on the nodular graphite of an EN-GJS grade cast iron. The shape is analyzed based on shape factor and aspect ratio as well as average particle size and distribution. The shape of graphites in cast iron materials is critical to the mechanical properties of these alloys and is essential for the characterization of the material. Metallographic serial sectioning creates a digital twin of the material, which is removed layer by layer and visualized layer by layer under an optical microscope. From this three-dimensional twin, any number of planes can be digitally projected and analyzed. Conventional quantitative 3D analysis examines the voxel count and composition of a body in 3D space. The analysis presented here applies stereological 2D analysis methods to three spatial planes of the material. Two of the planes are digital projections of the twin. The two reconstructed planes of the material are chosen so that the direction vectors of all three planes form an angle of 90° to each other. The methodology is described in detail and the challenges and opportunities of the serial section method presented here are discussed.

Transcriptional and secretome analysis of Rasamsonia emersonii lytic polysaccharide mono-oxygenases

The current study is the first to describe the temporal and differential transcriptional expression of two lytic polysaccharide monooxygenase (LPMO) genes of Rasamsonia emersonii in response to various carbon sources. The mass spectrometry based secretome analysis of carbohydrate active enzymes (CAZymes) expression in response to different carbon sources showed varying levels of LPMOs (AA9), AA3, AA7, catalase, and superoxide dismutase enzymes pointing toward the redox-interplay between the LPMOs and auxiliary enzymes. Moreover, it was observed that cello-oligosaccharides have a negative impact on the expression of LPMOs, which has not been highlighted in previous reports. The LPMO1 (30 kDa) and LPMO2 (47 kDa), cloned and expressed in Pichia pastoris, were catalytically active with (kcat/Km) of 6.6×10-2 mg-1 ml min-1 and 1.8×10-2 mg-1 ml min-1 against Avicel, respectively. The mass spectrometry of hydrolysis products of Avicel/carboxy methyl cellulose (CMC) showed presence of C1/C4 oxidized oligosaccharides indicating them to be Type 3 LPMOs. The 3D structural analysis of LPMO1 and LPMO2 revealed distinct arrangements of conserved catalytic residues at their active site. The developed enzyme cocktails consisting of cellulase from R. emersonii mutant M36 supplemented with recombinant LPMO1/LPMO2 resulted in significantly enhanced saccharification of steam/acid pretreated unwashed rice straw slurry from PRAJ industries (Pune, India). The current work indicates that LPMO1 and LPMO2 are catalytically efficient and have a high degree of thermostability, emphasizing their usefulness in improving benchmark enzyme cocktail performance.Key points• Mass spectrometry depicts subtle interactions between LPMOs and auxiliary enzymes.• Cello-oligosaccharides strongly downregulated the LPMO1 expression.• Developed LPMO cocktails showed superior hydrolysis in comparison to CellicCTec3.

Assessment of Minimally Invasive Surgical Techniques for Sagittal Craniosynostosis: A Multi-Center Time Series Study.

Minimally invasive strip craniectomy is a well-established treatment for sagittal craniosynostosis, however the temporality of change in head shape has not been assessed. In this study, we perform head shape analysis to compare time-series-based clinical outcomes between three different surgical techniques across three academic centers for the treatment of sagittal craniosynostosis. Retrospective, longitudinal 3D images were collected from patients who underwent surgery for the correction of sagittal craniosynostosis for up to 5 years post-operation. The surgical methods studied include spring-assisted craniectomy, narrow-strip craniectomy plus orthotic helmet therapy, and wide-strip craniectomy with biparietal and bitemporal barrel stave wedge osteotomies plus orthotic helmet therapy. Postoperative 3D images were binned into six age groups. Cranial index measurements were calculated on 3D images. 3D whole-head composite images were generated for each procedure to visually represent longitudinal outcomes. The median with interquartile range (IQR) CI measurements at 3-5 years postoperative follow up were 75.9 [73.1-78.6] for spring-assisted, 75.9 [75.4-78.5] for narrow-strip, and 79.4 [76.4-81.9] for wide-strip. The wide-strip CI was significanly different from the spring-assissted and narrow strip groups (p < 0.001). Concerning 3D analysis, spring-assisted patients showed normalization of frontal bossing and skull height compared to age-matched controls, while wide-strip patients showed greater correction of occipital bulleting. Narrow-strip patients had intermediate results between these outcomes. There was no statistically significant regression in longitudinal CI measurements across the three techniques aimed at treating sagittal craniosynostosis. Longitudinal comparison of 3D head shape outcome demonstrated satisfactory correction of scaphocephalic deformity across all three surgical groups.

Formation and Texture Analysis of Extrusion-Based 3D Printed Foods Using Nixtamalized Corn and Chickpea Flours: Effect of Cooking Process

Extrusion-based three-dimensional (3D) food printing (3DFP) enhances the customization of 3D-printed foods by using multiple food pastes. Post-printing processes like baking are usually necessary and significantly impact the stability of the 3D-printed foods. This study aimed to produce multi-material 3D-printed foods using nixtamalized corn dough and chickpea paste (CP) in extrusion-based 3DFP and to study the effect of post-printing processes (water oven cooking and steam cooking) and the type of material used (single- or multi-material) on the final appearance, weight, size, and texture of the 3D-printed foods. Multi-material 3D-printed foods were successfully produced using extrusion-based 3DFP. Steam-cooked 3D-printed foods cooked uniformly and had a better appearance, as they did not develop surface cracks compared to water oven-cooked foods. Water-oven cooked foods experienced a greater weight loss of 35.6%, and higher height and length reduction of 1.5% and 8.4%, respectively. Steam-cooked multi-material 3D-printed foods were harder at 40% of strain, with force values of 66.9 and 46.3 N for water-oven cooked foods. Post-printing processes, as well as the presence of CP in the middle of the 3D-printed foods, influenced their final appearance, weight, size, and texture. This study offers interesting findings for the innovative design of chickpea- and corn-based multi-material 3D-printed foods.