The COVID-19 pandemic has impacted urban life and created spatial and social inequalities in cities. The impacts of lifting full lockdown restrictions once fast-spreading and community-acquired infection waves were under control are still not fully understood. This study aims to explore spatial inequality reinforced in the intervals between the waves of infection during the COVID-19 pandemic. Enclave-reinforced inequality resulting from enclave-based lockdown policies in Chinese cities was investigated through an analysis of the impacts of university campus enclave closures on the accessibility and crowdedness of urban green spaces. Using a modified two-step floating catchment area (2SFCA) and inversed 2SFCA (i2SFCA) method, accessibility and crowdedness were calculated and compared under two different scenarios. Additionally, the Lorenz curve, Gini coefficient, and Theil index were used to measure and compare intra-city global and local inequalities under each scenario. The results indicate that the lockdown of university campus enclaves decreased the supply of urban green spaces. Campus closures not only exacerbated the unequal distribution of urban green space, but also reduced the inequality of crowdedness in urban parks due to increased crowdedness in parks near the closed enclaves. Moreover, both accessibility and crowdedness worsened when the calculations were weighted for population size and the total supply of green space. Enclave-based lockdown in cities reinforced spatial inequality, and it is highly complex and has multidimensional impacts on urban inequalities and environmental injustice which should be considered by urban planners and decision-makers hoping to create healthy, inclusive, resilient, and sustainable cities in the “new normal” of the COVID-19 pandemic.