24-hour Preservation Research Articles

Kidney storage at subzero temperatures using a hyperosmolar perfusate.

32 dog kidneys were stored in a hyperosmolar perfusate (770 mosm/kg) at minus2 and plus4 degrees C, respectively, for 12-48 h and thereafter autotransplanted. 18 kidneys of the control group were stored in Collins solution at plus4 degrees C. The immediate function of the kidneys after transplantation was measured by p-aminohippuric acid and inulin clearances. Successful 24-hour preservation was possible by use of the hyperosmolar perfusate. Kidneys stored in the hyperosmolar perfusate at minus2 degrees C were better preserved than kidneys stored in Collins solution at plus4 degrees C. The addition of human albumin to the hyperosmolar perfusate did not improve the preservation results.

Acidosis protection during 24-hour ischemic heart preservation

Puppies' hearts were preserved under hypothermic ischemic conditions. They were initially flushed with an intracellular type solution. It was shown that ATP stores were better maintained if the fluid was acid. The level of ATP at the end of 24 hr is compatible with recovery. In these conditions, the study of lactate production and tissue pH seems to indicate that it is due to a slower ATP decrease rate. The pH of the perfusate might act at the extracellular side of the myocardial cell. If hearts were reperfused after such a preservation, they resumed a sinus rhythm and strong contraction after isoproterenol infusion.

Perfusion in the experimental preservation of the rat heart.

Rat hearts have been preserved for 24 hours using four different preservation solutions. The results suggest that the best preservation fluid is Tyrode's solution with Ficoll 5 per cent. A comparison of simple cooling of the heart and perfusion, either continuous or intermittent, has shown that intermittent perfusion of the heart for 2 minutes every 6 hours gives the highest rate of 24-hour preservation.

The effect of furosemide on 24-hour hypothermic renal preservation

The protective effect of furosemide (Lasix) in renal ischemia has been studied in five groups of dogs. Each animal underwent removal of one kidney which was perfused with Collins' solution maintained at 4°C for 24 hr before autotransplantation. Contralateral nephrectomy was carried out at the time of the second operation in all groups. Furosemide was administered before kidney removal, in the perfusate, or immediately after transplantation or in various combinations of these. Maximum improvement in results was obtained when furosemide was administered intravenously in the posttransplantation period. On this basis we recommend clinical use of furosemide in the immediate posttransplant period.

Successful 24-hour preservation of the canine liver.

Liver preservation was attempted with 39 canine livers. Of 36 orthotopically transplanted livers, there were 17 failures because of anesthetic overdosage and respiratory problems. Nineteen dogs receiving livers preserved for 24 hours were eligible for evaluation of success of preservation. Twelve of the 19 dogs survived 5 to 70 days after transplantation, indicating successful preservation in most instances. Factors that may have contributed to success were administration of large doses of methylprednisolone to the donor prior to liver harvest, a relatively simple and efficient transplantation technique, low temperature of perfusion, and inclusion of high-dose methylprednisolone in the perfusate.

Ultrastructure of the porcine liver during a 24-hour simple hypothermic preservation

6 Schweinelebern wurden durch einfache Unterkuhlung 24 Std konserviert und die Strukturerhaltung in verschiedenen Zeitintervallen elektronenmikroskopisch und enzymcytochemisch untersucht. Fur die initiale hypotherme Kurzzeitperfusion diente eine mit Kalium und Glucose angereicherte modifizierte Ringer-Losung, der bei 3 Lebern zusatzlich sog. Zellstabilisatoren (Chlorpromazin und Prednisolon) hinzugefugt worden waren.

Ultrastructural Changes of Canine Kidneys During 24-Hour Perfusion on a LI-400 Preservation System

Short term experimental and clinical preservation of kidneys is presently best accomplished by hypothermic continuous pulsatile perfusion with cryoprecipitated and millipore filtered plasma. This study was undertaken to observe ultrastructural changes occurring during 24-hour preservation using the above mentioned method.A kidney was removed through a midline incision from healthy mongrel dogs under pentobarbital anesthesia. The kidneys were flushed immediately after removal with chilled electrolyte solution and placed on a LI-400 preservation system and perfused at 8-10°C. Serial kidney biopsies were obtained at 0-½-1-2-4-8-16 and 24 hours of preservation. All biopsies were prepared for electron microscopy. At the end of the preservation period the kidneys were autografted.

Successful 24-hour preservation of canine kidney with intracellular-type electrolyte solution

Successful 24-hour preservation of canine kidney with intracellular-type electrolyte solution

24-hour and 72-hour preservation of canine kidneys

24-hour and 72-hour preservation of canine kidneys