Purpose: Gluten-free diet (GFD) is the only effective treatment for celiac disease (CD). However, many gluten-free commercial products carry high energy and fat load to result more palatable and to improve bread-making. We aim to verify whether a GFD regimen may affect the lipid profile of children with CD. Methods: Any consecutive child on a GFD for at least 6 months was evaluated for lipid profile [total cholesterol, triglycerides, and high-density lipoprotein (HDL)], anthropometric data (weight, height and body mass index), and a 24-hour food diary during the standard follow-up visit. Results: Out of 60 children (age range 3-20 years), total cholesterol reached a level above 200 mg/dL in 6.7%, between 150-200 mg/dL in 55%, and below 150 mg/dL in the remaining 38%. The 24-hour food diary showed a cholesterol consumption above 300 mg/day in 13.3% of the children, between 200-300 mg/day in 13.3%, and below 200 mg/day in the remaining 73.4%. The ingested cholesterol and the saturated fat were not significantly correlated with cholesterol level and its fractions. Likewise, quantity of ingested fiber and blood cholesterol levels showed no significant correlation. Similar results were reached after correlating the amount of saturated and unsaturated fats present in the diet with HDL level, or after correlating simple sugar with triglycerides. After stratifying patients based on age, the 7-10 year-old children seemed to have the highest consumption of cholesterol, while the highest blood cholesterol levels were found in the age range 14-20. Conclusion: In conclusion, GFD does not seem to lead to an excessive intake of cholesterol although the effects of longer exposure should be specifically analyzed.