24-h Light Exposure Research Articles

Circadian Rhythm of Distal Skin Temperature in Healthy Older and Young Women and Its Relationship with Sleep-Wake Rhythm and Environmental Factors under Natural Living Conditions.

Little is known about the healthy aging of the circadian timing system under natural living conditions. This study explores changes in the circadian rhythm of distal skin temperature (DST) with aging and relates these changes to sleep-wake timing and environmental influences. DST, sleep-wake timing, 24-h light exposure, and physical activity were measured and averaged over seven consecutive days using temperature sensors, actigraphy with a light meter, and sleep diaries in 35 healthy older women (60-79 years) and 30 young women (20-34 years). Circadian rhythm characteristics, describing strength (amplitude) and timing (acrophase) of the DST rhythm, were calculated using cosinor analysis. The older adults displayed an 18-19% smaller amplitude and a 66-73 min earlier acrophase (peak time) for DST rhythm than the young adults, indicating a weaker and phase-advanced DST rhythm. The phase advance for DST was not due to an earlier evening increase, but to a shorter nocturnal plateau period. Daytime light exposure inversely affected strength (amplitude) but not phasing of the DST rhythm in older adults. The DST rhythm was 3.5 times more advanced than the sleep-wake rhythm, showing an altered phase relationship (phase angle) between both rhythms with aging. The phase angle was more heterogeneous among older adults, showing differential aging. The phase advance for DST rhythm and the altered and heterogeneous phase relationship between DST and sleep-wake rhythms were not related to ambient light exposure and the physical activity of older adults. This suggests that healthy aging of the circadian system might be due to endogenous mechanisms such as an internal rearrangement rather than external influences.

Solfeggio-frequency music exposure reverses cognitive and endocrine deficits evoked by a 24-h light exposure in adult zebrafish

Music therapy has long been used as a non-pharmacological intervention to improve cognitive function and mood in humans. Mounting rodent evidence also supports beneficial impact of music exposure on animal cognitive performance. The zebrafish (Danio rerio) is an important emerging aquatic animal model in translational biomedical and neuroscience research. Here, we evaluate the effects of intermittent (2-h or 6-h twice daily) and continuous (24-h) solfeggio-frequency music exposure on behavioral, cognitive and endocrine parameters in adult zebrafish whose circadian rhythm was disturbed by a 24-h light exposure. Overall, a 24-h light exposure stress evokes overt cognitive deficits in the inhibitory avoidance test and elevates zebrafish whole-body cortisol levels. However, these effects were reversed by solfeggio-frequency music exposure for 2 or 6 h twice daily, and by continuous 24-h exposure. Collectively, these findings suggest a positive modulation of cognitive and endocrine responses in adult zebrafish by environmental enrichment via the long-term exposure to music, and reinforces zebrafish as a robust, sensitive model organism for neurocognitive and neuroendocrine research.

Melatonin treatment reverses cognitive and endocrine deficits evoked by a 24-h light exposure in adult zebrafish

Melatonin is an important pineal hormone that regulates human and animal circadian rhythms and sleep. Mounting clinical and rodent evidence indicates that melatonin also modulates affective behaviors and cognition. The zebrafish (Danio rerio) is rapidly becoming a powerful novel model organism in translational neuroscience research. Here, we evaluate the effects of a 24-h melatonin treatment on behavior and physiology of adult zebrafish with circadian rhythm disturbed by a 24-h light exposure. While such light exposure evoked overt cognitive and neuroendocrine (cortisol) deficits in zebrafish, these effects were reversed by a 24-h melatonin treatment. Collectively, these findings suggest a positive modulation of affective and cognitive phenotypes in zebrafish by melatonin, and reinforce the growing utility of zebrafish models for studying circadian, cognitive and behavioral processes and their neuroendocrine regulation in vivo.

Light-exposure at night impairs mouse ovary development via cell apoptosis and DNA damage.

The alternation of light and dark rhythm causes a series of physiological, biochemical and metabolic changes in animals, which also alters the growth and development of animals, and feeding, migration, reproduction and other behavioral activities. In recent years, many studies have reported the effects of long-term (more than 6 weeks) illumination on ovarian growth and development. In the present study, we observed the damage, repair and apoptosis of ovarian DNA in a short period of illumination. The results showed that, in short time (less than 2 weeks) illumination conditions, the 24-h light treatment caused the reduction of total ovarian follicle number and down-regulation of circadian clock related genes. Furthermore, the changed levels of serum sex hormones were also detected after 24-h light exposure, of which the concentrations of LH (luteinizing hormone), FSH (follicle-stimulating hormone) and E2 (estradiol) were increased, but the concentration of PROG (progesterone) was decreased. Moreover, 24-h light exposure increased the expression of DNA damage and repair related genes, the number of TUNEL and RAD51 positive cells. These results indicated that 24-h light exposure for 4, 8 and 12 days increased DNA damage and cell apoptosis, thereby affecting the development of ovary.

The ipRGC-driven pupil response with light exposure and refractive error in children.

The intrinsically photosensitive retinal ganglion cells (ipRGCs) signal environmental light, control pupil size and entrain circadian rhythm. There is speculation that ipRGCs may be involved in the protective effects of light exposure in myopia. Here, the ipRGC-driven pupil response was evaluated in children and examined with light exposure and refractive error. Children ages 5-15years participated. Subjects wore an actigraph device prior to the lab visit for objective measures of light exposure and sleep. For pupillometry, the left eye was dilated and presented with stimuli, and the consensual pupil response was measured in the right eye. Pupil measurements were preceded by 5min dark adaptation. In Experiment 1 (n=14), 1s long wavelength light ('red,' 651nm, 167cdm-2 ) and 10 increasing intensities of 1s short wavelength light ('blue,' 456nm, 0.167-167cdm-2 ) were presented with a 60s interstimulus interval. A piecewise two-segment regression was fit to the stimulus response function to determine the functional melanopsin threshold. Pupil responses were analysed with light exposure over the previous 24h. For Experiment 2 (n=42), three 1s red and three 1s blue alternating stimuli were presented with a 60s interstimulus interval. Following an additional 5-min dark adaption, the experiment was repeated. Pupil metrics included peak constriction, the 6s and 30s post-illumination response (PIPR), early and late area under the curve (AUC). Following pupil measurements, cycloplegic refractive error and axial length were measured. For Experiment 1, PIPR metrics demonstrated a graded response to increasing intensity blue stimuli, with a mean functional melanopsin threshold of 6.2±4.5cdm-2 (range: 0.84-16.7cdm-2 ). The 6s PIPR and early AUC were associated with 24-h light exposure for high intensity stimuli (33.3 and 83.3cdm-2 , p<0.005 for both). For Experiment 2, there were no associations between pupil metrics and refractive error. The 6s PIPR and early AUC to blue stimuli were significantly increased for Trial 2 compared to Trial 1. The ipRGC-driven pupil responses in children were robust and similar to responses previously measured in an adult population. The 6s PIPR and early AUC to high intensity blue stimuli were associated with previous light exposure. There were no associations between the ipRGC-driven pupil response and refractive status in this cohort.

0299 TWENTY-FOUR HOUR LIGHT EXPOSURE PATTERN AND SLEEP CONSOLIDATION IN INSOMNIA PATIENTS

There is much evidence that links insufficient light exposure to sleep fragmentation. Previous studies reported that the exposure to lower light levels predicted more nighttime awakenings. This study aims to compare light exposure profiles across 24h between insomnia patients and normal controls, and to examine the relationship of light exposure pattern with sleep consolidation. Participants were recruited from three Public Health Centers in a rural area of Korea. The actigraphy recording for 7 days was conducted for each subject. One hundred six insomnia patients (62.27 ± 12.29 years) and 80 normal control (NC) subjects (55.64 ± 13.25 years) were included for our analysis. The raw light data across 24h were log transformed and averaged into hourly bins. The durations of exposure to four different light levels (< 10, 10 to <100, 100 to <1,000, and ≥1,000 lux) and the area under curve (AUC) values of 24h light exposure were obtained. The association of the duration of exposure to each light level with the wake after sleep onset (WASO) severity was examined using multinomial logistic regression analysis. In 24h light exposure profiles, the light intensity during the hour preceding 04h and 07h in the insomnia group was significantly higher than that of the NC group (p<0.05). There was no significant difference in the duration of exposure to each light level and the AUC of 24h light exposure between the insomnia and NC groups. In the combined group, the AUC was negatively correlated with WASO(r=-.22, p <0.01). The duration of exposure to each light level was not significantly associated with the presence of moderate and severe degree of WASO. Insomnia patients were exposed to higher light intensity in early morning hours compared to normal controls, although the light intensity across daytime was not different between the two groups. The sleep consolidation in community-dwelling adults would not be associated with the time spent at a certain light intensity, but with the total amount of 24h light exposure. Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology (2013R1A1A2009888).

Effect of differential photostimulation on induction of Fos-like immune-reactivity in the MBH region of Indian weaver bird

This study investigated the neuronal activation (c-fos) in the mediobasal hypothalamus (MBH) of the Indian weaver bird, after exposure to a single long-day. Wild-caught photosensitive birds were exposed to the short-days (LD 8L:16D). After four days of short-days photoperiod, the LD cycle was programmed such that the light illumination was continuous after zeitgeber time (ZT) 8. Birds were perfused on the same day at ZT 4 or ZT 20. Brains were processed for the immunohistochemistry of c-fos (Fos) in the MBH. We found a significant higher activation of Fos in neurons within the ventral tuberal division (containing infundibular nucleus) of the MBH, in group that received 20-h light than that received 4-h light on first long-day. But in the dorsal tuberal division, there was no noticeable difference in Fos-lir activation on after 4-h and 24-h light exposure. The results suggest that the ventral tuberal division of mediobasal hypothalamus is principally involved in detecting the photoperiodic information from the external environment, and hence, suggested as the key neural center, involved in the photoperiodic mechanism in Indian weaver bird.

A week in the life of full-time office workers: Work day and weekend light exposure in summer and winter

Little is known about the light exposure in full-time office workers, who spend much of their workdays indoors. We examined the 24-h light exposure patterns of 14 full-time office workers during a week in summer, and assessed their dim light melatonin onset (DLMO, a marker of circadian timing) at the end of the working week. Six workers repeated the study in winter. Season had little impact on the workers' schedules, as the timing of sleep, commute, and work did not vary by more than 30 min in the summer and winter. In both seasons, workers received significantly more morning light on workdays than weekends, due to earlier wake times and the morning commute. Evening light in the two hours before bedtime was consistently dim. The timing of the DLMO did not vary between season, and by the end of the working week, the workers slept at a normal circadian phase.

Fatty acid composition, fat-soluble vitamin concentrations and oxidative stability in bovine milk produced on two pastures with different botanical composition

Previous research has shown that grazing pastures compared to feeding preserved forages has large impact on milk fatty acid (FA) composition, but differences between grazing red clover (Trifolium pratense L.) or white clover (Trifolium repens L.) are small, whereas the herbage proportions of dicotyledon botanical families is positively correlated with the milk-fat proportions of total polyunsaturated FA when grazing pastures in the Alps. The objective of the present study was to investigate the influence of botanically different pastures on bovine milk composition and milk susceptibility to oxidation. Two groups of 8 multiparous Norwegian Red dairy cows [mean (standard deviation); 599 (45.1)kg body weight, 73 (15.0)d in milk, 29.9 (2.90)kg milk/d at experiment start] grazed either a short-term pasture (SP) or a long-term pasture (LP). Both pastures were organically managed, meaning that no artificial fertilizers or herbicides were applied. The SP was representative for pastures, which are frequently, i.e. at least every third year, renewed by soil tillage and seeding, whereas LP was representative for pastures, which are less frequently renewed. The SP contained mainly meadow fescue (Festuca pratensis Huds.), timothy (Phleum pratense L.) and red clover and LP contained smooth meadow grass (Poa pratensis L.), white clover and a variety of unsown species. Sixteen cows were blocked according to milk yield, days in milk and sire, and randomly within block allocated to the 2 dietary treatments with a daily pasture allowance of 15–20kg dry matter per cow, supplemented with 3.0kg barley (Hordeum vulgare L.) concentrate. Milk was sampled during the last week of 3 experimental periods and analysed for FA composition by gas chromatography, concentrations of fat-soluble vitamins by high performance liquid chromatography, and oxidative stability in a light-exposure experiment by measuring the formation of hydroperoxides and by front-face fluorescence spectroscopy. Pasture type had no effect on milk yield, milk gross composition, and only minor effects on milk FA composition. Milk from SP had higher concentration of α-tocopherol than LP. The formation of hydroperoxides in milk was lower for SP than LP after 24h light exposure, but no differences were found after 48h. Front-face fluorescence spectroscopy revealed slightly higher formation of components in the area of 409–480nm wavelength for SP than LP, which may be related to milk-lipid oxidation. The experimental pastures differed mainly in herbage proportions of red clover and white clover and less in proportions of non-legume dicotyledons. This explains small differences in milk FA composition and milk susceptibility to oxidation.

Melatonin Production and Light Exposure of Rotating Night Workers

Decreased melatonin production, due to acute suppression of pineal melatonin secretion by light exposure during night work, has been suggested to underlie higher cancer risks associated with prolonged experience of night work. However, the association between light exposure and melatonin production has never been measured in the field. In this study, 24-h melatonin production and ambulatory light exposure were assessed during both night-shift and day/evening-shift periods in 13 full-time rotating shiftworkers. Melatonin production was estimated with the excretion of urinary 6-sulfatoxymelatonin (aMT6s), and light exposure was measured with an ambulatory photometer. There was no difference in total 24-h aMT6s excretion between the two work periods. The night-shift period was characterized by a desynchrony between melatonin and sleep-wake rhythms, as shown by higher melatonin production during work and lower melatonin production during sleep when working night shifts than when working day/evening shifts. Light exposure during night work showed no correlation with aMT6s excreted during the night of work (p > .5), or with the difference in 24-h aMT6s excretion between the two work periods (p > .1). However, light exposure during night work was negatively correlated with total 24-h aMT6s excretion over the entire night-shift period (p < .01). In conclusion, there was no evidence of direct melatonin suppression during night work in this population. However, higher levels of light exposure during night work may have decreased total melatonin production, possibly by initiating re-entrainment and causing internal desynchrony. This interpretation is consistent with the proposition that circadian disruption, of which decreased melatonin production is only one of the adverse consequences, could be the mediator between night shiftwork and cancer risks. (Author correspondence: marie.dumont@umontreal.ca)

Morphological Changes in the Pineal Gland of Rats under Conditions of Long-Term Exposure to Bright Light

Changes in the diurnal light cycle affect the morphofunctional state of the pineal gland. The volume of the nucleus, Golgi apparatus, and mitochondria in pinealocytes decreases after 45-day exposure to bright light. After 90 days, the degree of nuclear polymorphism increased, the specific volume of the Golgi apparatus returned to normal, the volume of the granular endoplasmic reticulum decreased, while the volume of lysosomes, free ribosomes, and polysomes increased. These changes reflect plasticity of pinealocytes and adaptation of the gland to long-term 24-h light exposure.

Twenty-four hours light exposure experiences in postpartum women and their 2–10-week-old infants: An intensive within-subject design pilot study

Background Adequate light exposure is critical for entraining circadian rhythms, regulating sleep–wake cycles, and maintaining optimal mood. Yet, few studies have reported normative data on light exposure experiences in postpartum women and young infants; none has examined the two simultaneously. Objectives The objective of this pilot study was to document the 24-h light exposure experiences in postpartum women and their infants. Design An intensive within-subject design was employed. Settings The participant’s natural home environment. Participants Twenty-four healthy mother–infant pairs were recruited from the general community in Seattle, USA. Two mother–infant pairs did not have complete data, resulting in a final sample size of 22 (12 female infants). Methods Mothers and infants wore a monitor to continuously record illumination levels for 7 days. Data were aggregated within subject to calculate summary measures of illumination exposure. Circadian patterns of light were examined using cosinor analysis. Pearson correlation was used to examine the relation between maternal and infant light exposure. Results Mothers spent 71.13 ± 11.58% and infants spent 80.07 ± 8.27% of their daytime hours (defined as 06:00–21:59) in an illumination level <50 lux. Mean minutes >1000 lux per day was 54 ± 39 for mothers and 23 ± 18 for infants. Maternal and infant light exposure exhibited a modest circadian pattern and a strong correlation, both in the timing of peak illumination exposure ( r = 0.93, p < 0.01) and in the level of light exposure ( r = 0.70 ± 0.07). Conclusions Postpartum women and infants experience low ambient light levels with short periods of bright light during the day. Whether this level of light is sufficient for optimal postpartum mood and infant circadian entrainment warrants further investigation. If higher levels of light were indeed necessary for postpartum women and young infants, increasing ambient light levels through the use of natural sunlight (i.e., walking outdoors) could be a simple and economic nursing intervention. The strong pattern synchrony between maternal and infant light exposure suggests that mothers play the primary role in providing a lighting environment suitable for infants to synchronize their circadian rhythms to a 24-h day.

Light exposure causes functional changes in the retina: increased photoreceptor cation channel permeability, photoreceptor apoptosis, and altered retinal metabolic function

Light exposure induces retinal photoreceptor degeneration and retinal remodeling in both the normal rat retina and in animal models of retinal degeneration. Although cation entry is one of the triggers leading to apoptosis, it is unclear if this event occurs in isolation, or whether a number of pathways lead to photoreceptor apoptosis following light exposure. Following light exposure, we investigated the characteristics of cation entry, apoptotic markers [using terminal deoxynucleotidyl transferase (EC 2.7.7.31) dUTP nick-end labeling (TUNEL) labeling] and metabolic properties of retina from Sprague-Dawley (SD) rats and a rat model of retinitis pigmentosa [proline-23-histidine (P23H) rat]. Assessment of cation channel permeability using agmatine (AGB) labeling showed that excessive cation gating accompanied the series of anomalies that occur prior to photoreceptor loss. Increased AGB labeling in photoreceptors was seen in parallel with the appearance of apoptotic photoreceptors detected by TUNEL labeling with only a smaller proportion of cells colocalizing both markers. However, SD and P23H retinal photoreceptors differed in the amounts and colocalization of AGB gating and TUNEL labeling as a function of light exposure. Finally, reduced retinal lactate dehydrogenase levels were found in SD and P23H rat retinas after a 24-h light exposure period. Short-term (2 h) exposure of the P23H rat retina caused an increase in lactate dehydrogenase activity suggesting increased metabolic demand. These results suggest that energy availability may be exacerbated during the early stages of light exposure in susceptible retinas. Also, the concomitant observation of increased ion gating and TUNEL labeling suggest the existence of at least two possible mechanisms in light-damaged retinas in both SD and the P23H rat retina.

Daily Light Exposure in Morning-Type and Evening-Type Individuals

Morning-type individuals (M-types) have earlier sleep schedules than do evening types (E-types) and therefore differ in their exposure to the external light-dark cycle. M-types and E-types usually differ in their endogenous circadian phase as well, but whether this is the cause or the consequence of the difference in light exposure remains controversial. In this study, ambulatory monitoring was used to measure 24-h light exposure in M-type and E-type subjects for 7 consecutive days. The circadian phase of each subject was then estimated in the laboratory using the dim-light melatonin onset in saliva (DLMO) and the core body temperature minimum (Tmin). On average, M-types had earlier sleep schedules and earlier circadian phases than E-types. They also showed more minutes of daily bright light exposure (> 1000 lux) than E-types. As expected, the 24-h patterns of light exposure analyzed in relation to clock time indicated that M-types were exposed to more light in the morning than E-types and that the reverse was true in the late evening. However, there was no significant difference when the light profiles were analyzed in relation to circadian phase, suggesting that, on average, the circadian pacemaker of both M-types and E-types was similarly entrained to the light-dark cycle they usually experience. Some M-types and E-types had different sleep schedules but similar circadian phases. These subjects also had identical light profiles in relation to their circadian phase. By contrast, M-types and E-types with very early or very late circadian phases showed large differences in their profiles of light exposure in relation to their circadian phase. This observation suggests that in these individuals, early or late circadian phases are related to relatively short and long circadian periods and that a phase-delaying profile of light exposure in M-types and a phase-advancing profile in E-types are necessary to ensure a stable entrainment to the 24-h day.

Association of morning illumination and window covering with mood and sleep among post-menopausal women.

The antidepressant and sleep-promoting effects of light exposure might be useful for treating age-related mood and sleep disorders. In view of recent evidence suggesting beneficial effects of morning light, this study examined the associations of mood and sleep with morning light exposure, 24 h environmental illumination, and the degree to which the volunteers' bedroom windows were covered in the morning. We examined 459 postmenopausal women participating an ancillary study of the Women's Health Initiative conducted at the University of California, San Diego Clinical Center, San Diego, CA, USA. At baseline, volunteers completed a 4-week sleep-recall questionnaire. Volunteers were then assessed for 5-7 days in their home environments with actigraphic wrist monitors. During home recording, self-reported mood was assessed. Morning illumination during the first 4 h after arising, 24-h illumination mesor (cosine-fitted mean), and illumination acrophase (cosine-fitted peak time) were calculated. Sleep was scored each night using validated wrist actigraphic methods. A sleep diary was completed each morning. During two 24-h periods, urine was collected approximately every 2 h during wakefulness and following any voidings during the sleep period. Cosine-fitting established the acrophase of urinary 6-sulfatoxymelatonin (aMT6s) excretion. Morning illumination and 24-h illumination were modestly associated with better mood and sleep. Associations of light with mood and sleep were consistently greater for subjects whose body clocks were delayed relative to the group median. Less morning window covering in the subjects' bedrooms was associated with more morning light and less depressed mood. The results suggest that both morning and 24-h light exposure may be beneficial for older adults.

Profile of 24-h light exposure and circadian phase of melatonin secretion in night workers.

Light exposure was measured in 30 permanent night nurses to determine if specific light/dark profiles could be associated with a better circadian adaptation. Circadian adaptation was defined as a significant shift in the timing of the episode of melatonin secretion into the daytime. Light exposure was continuously recorded with ambulatory wrist monitors for 56 h, including 3 consecutive nights of work. Participants were then admitted to the laboratory for 24 h where urine was collected every 2 h under dim light for the determination of 6-sulphatoxymelatonin concentration. Cosinor analysis was used to estimate the phase position of the episode of melatonin secretion. Five participants showed a circadian adaptation by phase delay ("delayed participants") and 3 participants showed a circadian adaptation by phase advance ("advanced participants"). The other 22 participants had a timing of melatonin secretion typical of day-oriented people ("nonshifters"). There was no significant difference between the 3 groups for total light exposure or for bright light exposure in the morning when traveling home. However, the 24-h profiles of light exposure were very distinctive. The timing of the main sleep episode was associated with the timing of light exposure. Delayed participants, however, slept in darker bedrooms, and this had a major impact on their profile of light/dark exposure. Delayed and advanced participants scored as evening and morning types, respectively, on a morningness-eveningness scale. This observation suggests that circadian phase prior to night work may contribute to the initial step toward circadian adaptation, later reinforced by specific patterns of light exposure.

Paradoxical role of ascorbic acid and riboflavin in solutions of total parenteral nutrition: implication in photoinduced peroxide generation.

In the presence of light, a multivitamin preparation is the main source of peroxides in solutions of total parenteral nutrition (TPN). This preparation contains two photosensitive products, 5'-phosphate flavin mononucleotide (FMN) and polysorbates (PS), as well as electron donors such as ascorbate (AH). We hypothesized that the admixture of FMN or PS with electron donors generates peroxides in TPN and alters the quality of nutrients. Using xylenol orange, peroxide concentrations were measured in solutions containing AH, FMN, and/or PS in water, a dextrose solution, an amino acid preparation, and a lipid emulsion. Thiol functions were evaluated by reduction of 5,5-dithiobis(2-nitrobenzoic acid) in the amino acid preparation. After 24-h light exposure, dextrose solutions with admixtures of AH + FMN or AH + FMN + PS generated peroxides at concentrations similar to those observed in a 1% multivitamin solution, and over three times higher than those observed with FMN, PS, or AH alone. However, in the presence of amino acids, FMN alone induced a generation of peroxides comparable to that observed with FMN + AH. In the lipid emulsion, peroxides increased over 3-fold in the presence of FMN or FMN + AH. The addition of catalase suggested that lipid peroxides and H2O2 were produced, and the loss of thiol function suggested that an oxidation of amino acids occurred. When exposed to light, FMN induces reactions with amino acids, polyunsaturated fatty acids, and even AH, altering the quality of nutrients. Paradoxically, AH without FMN has a protective effect on peroxide generation in TPN.

An immunohistochemical study of opsin in photoreceptor cells following light-induced retinal degeneration in the rat.

Light-induced retinal degeneration has been hypothesized to be rhodopsin-mediated. However, the alterations induced in the opsin moiety of the rhodopsin molecule and its distribution in the rod cell after a photic insult have not been definitively established. We used light and electron immunohistochemistry to study the alterations in retinal opsin immunoreactivity in a rat model of retinal photic injury. In normal unexposed rat retinas, opsin immunoreactivity was restricted to the rod outer segments. At 6 h after a 24-h light exposure, opsin immunoreactivity was present in the rod outer segments in both the superior and inferior retina, but in addition marked immunoreactivity was present in the inner segments in the superior quadrant of the light-damaged retina. At 6 days after exposure, intense immunoreactivity was noted around the severely degenerating rod nuclei and inner segments. However, at 21 days following light exposure, opsin immunoreactivity in areas of recovery was again restricted to the short regenerated rod outer segments. It appears that, despite severe light-mediated retinal degeneration, anti-opsin immunoreactivity persisted in the photoreceptor cells but with an altered pattern in damaged rod outer segments and photoreceptor perikarya. However, opsin immunoreactivity relocated to the regenerated rod outer segments in the recovery phase.

Dark reversion of blue-light inhibition of conidiation in Alternaria cichorii

Prolonged exposure to blue light was necessary for almost complete inhibition of sporulation in Alternaria cichorii Nattrass. When a 24-h blue light exposure was followed by darkness, the inhibition of sporulation decreased gradually.

Transient increase in 3H-fucose incorporation following first exposure of dark-reared rats to light

Incorporation of 3H-fucose into soluble and particulate glycoproteins of the visual and motor cortices of dark-maintained, light-exposed and normal rats was compared. Exposure to light for 1h had no effect but exposure for 3h led to a 23% elevation of incorporation into the particulate fraction of the visual cortex of light-exposed as compared to dark-maintained controls. However a 24h light exposure led to a depression in incorporation in this fraction from light-exposed rats to 83% that of the dark-maintained animals. These results demonstrate that a transient increase in synthesis of particulate glycoproteins occurs following first exposure to light followed by an increase in degradative processess leading to their breakdown.