ObjectiveTo compare the incidence of hypotony after oblique and straight sclerotomies in 25-gauge transconjunctival sutureless vitrectomy (TSV). DesignRetrospective, comparative, interventional case series. ParticipantsTwo hundred seventy-five eyes of 250 patients. MethodsWe reviewed 275 consecutive cases of 25-gauge TSV. Oblique incisions were performed on 141 eyes and straight incisions on 134 eyes. ResultsOn postoperative day 1, there was no significant difference in the incidence of hypotony (9.9% vs. 9.2%; p = 0.85) between oblique and straight incisions. The type of incision showed no statistical effect on hypotony in eyes filled with fluid (p = 0.91) or air-gas (p = 0.81). Hypotony was significantly reduced using air-gas endotamponade (4.8%) as compared to fluid-filled eyes (20.0%; p = 0.0001). ConclusionOblique incisions do not reduce the incidence of postoperative hypotony in fluid-filled eyes or with air-gas tamponade. Air-gas tamponade use results in a 4-fold reduction in the incidence of hypotony.