28th Day Research Articles

Chitosan (CTS) induced secondary metabolite production in Canscora decussata Schult. - An endangered medicinal plant

Bioengineering has excellent potential in plant-based in vitro cultures for efficiently synthesizing desired plant products. Due to the rapid destruction of natural habitats, in vitro methods might save endangered species. Winged-Stem Canscora is an endangered medicinal herb used in traditional medicine for brain disorders. This study investigates the induction of in-vitro metabolite production using chitosan. Among the various concentrations of auxins tested, 82% of callus was obtained from Murashige and Skoog (MS) medium with 3 mg L−1 Kinetin (KIN) + 1 mg L−1 1-Naphthaleneacetic acid (NAA). The highest callus content was after the 3rd subculture at 200 mg L−1 chitosan treatment. On the 70th day of culture, levels of total phenolics and flavonoids are increased in the elicited and normal callus. However, antioxidant activity was higher in elicited callus compared to normal callus and wild plants. Higher secondary metabolite concentrations of elicited callus have superior anti-bacterial and anti-fungal activity. Secondary metabolites from elicited callus have a lower inhibitory concentration than those from wild plant and normal callus. High-performance thin layer chromatography (HPTLC) analysis showed that the elicited callus had higher amounts of mangiferin (0.178 μg mL−1) and scopoletin (0.133 μg mL−1). An efficient approach was employed to ensure that sufficient amounts of the widely used plant secondary metabolites from medicinal plants, such as mangiferin and scopoletin, were accessible to meet demand.

Feeding protocol for the Amazonian ornamental cichlid Apistogramma cacatuoides and a histological approach to the nutritional condition of larvae.

The aim of this study was to develop a feeding protocol for the larviculture of Apistogramma cacatuoides, using the histological approach to larval nutrition conditions. For this, three experiments were carried out. Experiment 1 was carried out in a randomized design to determine the optimal amount of Artemia nauplii (AN) per larva, and three treatments were evaluated: P1-feeding with 25 A. nauplii per larva (AN/L) during the first 5 days, followed by 50 AN/L from the 6th to the 10th day and 100 AN/L from the 11th to the 20th day; P2 and P3-37 and 50 AN/L during the first 5 days, 75 and 100 AN/L from the 6th to the 10th day and 150 and 200 AN/L from the 11th to the 20th day. Experiment 2 was carried out in a randomized design to determine the daily frequency of feeding and evaluated four feeding frequencies: F1-feeding only once a day (09:00); (F2)-feeding twice a day (09:00 and 17:00); F3-feeding three times a day (09:00, 11:30 and 17:00); and F4-feeding four times a day (09:00, 11: 30, 14:00 and 17:00). Experiment 3 lasted 40 days and was conducted in a randomized design to evaluate three periods for the beginning of the feeding transition: WE10:AN for 10 days, followed by 3 days of co-feeding and commercial feed until the end of the experimental period; WE15:AN for 15 days, followed by 3 days of co-feeding and commercial feed; WE20:AN for 20 days, followed by 3 days of co-feeding and commercial feed. The results of this study showed that, for the best development of the larvae, they should receive the feeding protocol 50-100-200 AN/L (P3) until the 20th day of exogenous feeding. From the 21st day, the transition to inert food should begin with 3 days of co-feeding, and feeding during larviculture should be carried out at a frequency of twice a day; this protocol provided a good nutritional status for the larvae, as shown by the histological approach.

ЦИРКОВИРУС-МИКОПЛАЗМЕННАЯ ИНФЕКЦИЯ ПОРОСЯТ В ЭКСПЕРИМЕНТЕ

The aim of the study is to investigate the manifestation of circovirus-mycoplasma infection in piglets both as a mixed infection and as a monoinfection in the experiment. Objectives: to study the interaction of circovirus type 2 with Mycoplasma hyopneumoniae in the body of weaned piglets, as well as the manifestation of monoinfection in piglets during experimental infection; to assess the pathogenicity of PCV2d/M.hyo mixed infection, PCV2d monoinfection and M. hyo monoinfection; to identify the clinical and pathomorphological manifestation of mixed and single circovirus and mycoplasma infection in the experiment. Four groups of 23-day-old piglets (5 heads each) were formed and tested for the absence of pathogens. Experimental group I – mixed circovirus-mycoplasma infection, experimental group II – PCV2d monoinfection, experimental group III – M.hyo monoinfection, group IV – control. After infection, rectal temperature was measured daily and clinical manifestations were observed, nasal swabs were taken to determine the number of copies of M. hyo nucleic acid, blood was taken from each piglet weekly to detect viral nucleic acid and antibodies. All experimental and control piglets were euthanized on the 35th day from the beginning of the experiment. Pathomorphological and pathohistological studies were carried out. The experimental study reveals the synergistic pathogenicity of PCV2d and M. hyo in mixed infection. In experimental infection of weaning piglets with both M. hyo monoinfection and M. hyo/PCV2d mixed infection, it was found that M. hyo nucleic acid was detected in nasal smears in 80 % of animals and in lung tissue in 100 %. The number of copies of the PCV2d circovirus genome in the inguinal lymph node tissue of piglets in the mixed infection group was higher than in the monoinfection group. Circovirus type 2 in piglets can proceed latently, and circovirus-mycoplasma infection can be a complex respiratory disease.

МОРФОГЕНЕЗ ЯИЧНИКОВ ЦЫПЛЯТ-БРОЙЛЕРОВ ПРИ ИСПОЛЬЗОВАНИИ КОРМОВОЙ ДОБАВКИ «ЯНТАРНЫЙ ХОЛОДОК»

The aim of research is to study the development of structural and functional features of the reproductive system of broiler chickens using the feed additive Yantarnyj holodok. Research was conducted on broiler chickens of the Ross-308 cross under experimental conditions, while for the experiment, control and experimental groups of 30 heads each were created, starting from the day-old age, broiler chickens of the control group received tap water from vacuum drinkers without adding a feed additive, and chickens of the experimental group were given the feed additive Yantarnyj holodok daily in a dose of 1.5 l / t with drin¬king water until the end of growing. As a main diet, broiler chickens of both groups received compound feed of Ptitsefabrika Artemovskaya LLC. The use of the feed additive Yantarnyj holodok had a positive effect on the live weight gain, which by the end of the experiment was 2,938.10 ± 405.34 g, which was 1.9 % higher than the average value in the control group. 100 % survival of broiler chickens in the experimental group was established. Histological studies revealed the process of gradual improvement of the ovary structure, the formation of its cortical and cerebral zones. At the age of 10 days, the histological structure of the ovary was not formed and represented only the protein coat, and the internal part was represented by chaotically scattered follicles, among which follicles in the pertification state were found. By the 20th day, the cortical and medullar zones of the ovary could be seen. By the 35th day, the formation of egg-bearing mounds with a zone of formed follicles of varying degrees of maturity is observed, but complete maturation of follicles with a formed egg cell was not detected in the studied individuals. The medullar zone of the ovary is rich in blood vessels of different calibers, that is, the vascularization of the ovary of chickens of this age is significantly developed.

УВЕЛИЧЕНИЕ ХРАНИМОСПОСОБНОСТИ ШОКОЛАДНЫХ КОНФЕТ ЗА СЧЕТ СНИЖЕНИЯ АКТИВНОСТИ ВОДЫ В НАЧИНКАХ-ГАНАШАХ

According to research by NielsenIQ, the confectionery group is the most rapidly growing one in the food market of our country. The water activity aw value in confectionery has a significant impact on the shelf life of cased sweets with ganache filling. The objective of the study was to develop a method for increasing the shelf life of chocolate ganache fillings by reducing water activity and optimally selecting hydrophilic recipe components while maintaining high organoleptic properties of cased sweets. The objects of the study were: control ganache without adding additional sugars; model samples of ganaches with hydrophilic agents, which included invert syrup, dextrose and sorbitol, taken in the ratio 1:1:1.5 (sample 1); 1:1.5:1 (sample 2); 1:1:1 (sample 3) with 12 % fruit puree replaced. During the studies, the water activity of sample 1 was reduced by 28 %, which allowed the candy to be stored for 60 days with a safety factor of 1.3. The sorbitol content in a ratio of 1.5 to dextrose and invert syrup provided the best microbiological stability, while in the control, which had a water activity of 0.97 %, the QMAFAnM indicators were already exceeded on the 20th day of storage. During organoleptic evaluation it was determined that different contents of invert syrup, dextrose and sorbitol formed different textures of ganache: the control sample had a denser consistency, traces of emulsion stratification, while the model samples retained a plastic, homogeneous filling, and sample 3 had the best flavor throughout the entire shelf life (4.45 points on the 60th day of sto¬rage compared to 4.27 and 4.35 points for the 2nd and 3rd samples, respectively). It is shown that the shelf life of ganache-containing candies for 60 days with preservation of organoleptic indicators can be achieved with a ratio of invert syrup, dextrose and sorbitol as 1:1:1.5.

Cardioprotective role of long-term kefir and omega-3 fatty acid supplementation on myocardial apoptosis via oxidative stress-mediated lysosomal cathepsin release in isoproterenol-induced myocardial infarction rat model

Objective: The antiapoptotic and antioxidative role of long-term kefir and omega-3 fatty acids and their relationship with cysteine proteases on isoproterenol (ISO) induced myocardial infarction (MI) experimental model was investigated in our study. Material and Methods: Fifty male Sprague-Dawley rats were evenly divided into five distinct groups (n=10): Control, MI, kefir +MI, omega-3+MI, and kefir+omega 3+MI groups. Kefir 10% (with drinking water) and omega-3 fatty acid (30 mg/day per 100g body weight into the standard chow) were administrated during 30 days. ISO was subcutaneously injected into the rats (100 mg/ kg b.w.) on the 29th and 30th days. Myocardial tissue and blood samples were taken 12 hours after the last ISO dose. Creatine kinase MB (CK-MB) activities were measured in serum samples. Caspase-3, superoxide dismutase (SOD), malondialdehyde (MDA), nitric oxide (NO), DNA fragmentation, cathepsin B and L levels, were measured in myocardial tissue. Results: Serum CK-MB (p<0.05) and cardiac tissue MDA (p>0.05), NO (p<0.01), caspase 3 (p<0.01), DNA fragmentation (p<0.001), cathepsin B (p<0.05) and L (p<0.05) activities were increased and SOD (p<0.001) activities were decreased in MI group compared to control group. The preventive effects of long-term therapy with kefir and omega-3 fatty acids have been demonstrated on apoptosis, oxidative stress markers, and cysteine protease enzymes. Conclusion: Our results showed that long-term administration of kefir and omega-3 fatty acids might be effective in reducing myocardial apoptosis through oxidative stress-mediated release of cysteine proteases in myocardial infarction, especially in the kefir and combined therapy groups.

Management of Corneal Ulcers using Autologous Serum, Platelet Rich Plasma and Processed Human Amniotic Membrane in Dogs

Background: In dogs the corneal ulcers are one of the major ophthalmic emergencies and can cure with routine medications, but in delay presentation will become challenge for veterinarians. In such cases advanced therapeutic management such as serum, platelet reach plasma and corneal grafts are needed for favorable outcome. Methods: The present study was conducted on 24 dogs (29 eyes) with corneal ulcer categorized as a superficial (Group-I, n=15), deep corneal ulcers (Group-II, n=8) and descemetocele (Group-III, n=6) which were treated with autologous serum, Platelet Rich Plasma and Processed decellularized human amniotic membrane grafting, respectively. All the affected eyes were given routine topical medications with eye drops Moxifloxacin, Flurbiprofen q.i.d till healing, 1% Atropine sulphate b.i.d for 5 days and Oral Doxycycline was given @ 5 mg/kg b.i.d for 10 days. Result: The group-I (n=11, 15 eyes) treated with autologous serum eight to ten times-a-day as per severity with routine medication. Two dogs with entropion which was corrected first. The eyes gained vision within 10th-15th days. Developed complications managed medicinally till complete recovery. The group-II (n=7, 8 eyes) treated with platelet rich plasma drops five to seven times-a-day with routine medication. 75% eyes recovered up to 45th day. Developed complication were managed medicinally till complete recovery. The group-III (n=5, 6 eyes) treated with Decellularized Processed Human Amniotic Membrane Graft. 4 eyes gained complete transparency on 28th day of therapy while in 2 eyes developed complication were managed medicinally.

Fluoride release and antibiofilm of Alkasite restorative materials

This invitro study assessed fluoride ion release and antibiofilm activity of Alkasite materials against two types of bacterial spp. (Streptococcus mutans and Lactobacillus plantarum). Three different ion-releasing materials (Cention N, Cention Forte and Fuji IX GP) were used to make four groups. A total of 96 disc-shaped samples were prepared in polyethene mould, 32 samples (n=8) were used for evaluating fluoride ion release the samples were placed in vial containing 5ml of deionized water, and the measurements were recorded in four periods (1,7,21 and 28) days by utilizing an ion-selective electrode (ISE). 64 samples were used to evaluate the anti-biofilm activity of the materials groups against (S. mutans and L.plantarum), by using crystal violet assay for quantitative biofilm assessment (n=5 of each group for each bacterial spp.) and by SEM for the qualitative evaluation (n=3 of each group for each bacterial spp). Fuji IX GP registered the highest amount of fluoride release on 1st day of the observation period than Cention N and No primer was added to Cention Forte. On the 28th day, Primer added Cention Forte recorded the highest mean value of fluoride release among all of the groups. Primer-added Cention Forte has significantly more biofilm accumulation of both (Streptococcus mutans and Lactobacillus plantarum) than Cention N and No primer-added Cention Forte. Fuji IX showed significantly higher Streptococcus mutans biofilm accumulation compared to Cention N. Alkasite restorative materials showed a low potential for biofilm accumulation regarding both Streptococcus mutans and Lactobacillus plantarum.

Auricular cartilage regeneration using chondroitin sulfate-based hydrogel with mesenchymal stem cells in rabbits.

Cartilage is an avascular and alymphatic tissue that lacks the intrinsic ability to undergo spontaneous repair and regeneration in the event of significant injury. The efficacy of conventional therapies for invasive cartilage injuries is limited, thereby prompting the emergence of cartilage tissue engineering as a possible alternative. In this study, we fabricated three-dimensional hydrogel films utilizing sodium alginate (SA), gelatin (Gel), and chondroitin sulfate (CS). These films were included with Wharton's jelly mesenchymal stem cells (WJ-MSCs) and intended for cartilage tissue regeneration. The hydrogel film that were prepared underwent evaluation using various techniques including scanning electron microscope (SEM), Fourier transform infrared (FTIR) spectroscopy, assessment of the degree of swelling, degradation analysis, determination of water vapor transmission rate (WVTR), measurement of water contact angle (WCA), evaluation of mechanical strength, and assessment of biocompatibility. The rabbit ear cartilage regeneration by hydrogel films with and without of WJ-MSCs was studied by histopathological investigations during 15, 30, and 60 days. The hydrogel films containing CS exhibited superior metrics compared to other nanocomposites such as better mechanical strength (12.87 MPa in SA/Gel compared to 15.56 in SA/Gel/CS), stability, hydrophilicity, WVTR (3103.33 g/m2/day in SA/Gel compared to 2646.67 in nanocomposites containing CS), and swelling ratio (6.97 to 12.11% in SA/Gel composite compared to 5.03 to 10.90% in SA/Gel/CS). Histopathological studies showed the presence of chondrocyte cells in the lacunae on the 30th day and the complete restoration of the cartilage tissue on the 60th day following the injury in the group of SA/Gel/CS hydrogel containing WJ-MSCs. We successfully fabricated a scaffold composed of alginate, gelatin, and chondroitin sulfate. This scaffold was further enhanced by the incorporation of Wharton's jelly mesenchymal stem cells. Our findings demonstrate that this composite scaffold has remarkable biocompatibility and mechanical characteristics. The present study successfully demonstrated the therapeutic potential of the SA-Gel-CS hydrogel containing WJ-MSCs for cartilage regeneration in rabbits.

Study on Quality Changes of Kelp Gel Edible Granules during Storage.

The kelp gel edible granules developed utilizing the gel properties of alginate are prone to quality deterioration if improperly stored during the storage process. This study comprehensively investigated the quality changes of kelp gel edible granules stored at 4 °C and 25 °C by evaluating indicators such as total bacterial count, coliform bacteria, pH, relaxation time, color difference, appearance, texture characteristics, gel strength, and sensory scoring. The results showed that during the storage at 4 °C, the total bacterial count remained within the national standard range, the hardness and chewiness increased, the gel strength first increased and then decreased, the partial exudation of the bound water in the product occurred, and the sensory score slightly decreased, with an overall minor change in quality. During the storage at 25 °C, significant quality changes were observed, with the total bacterial count exceeding the national standard on the 20th day; additionally, the hardness, chewiness, and gel strength all initially increased and then decreased, both the bound water and the restrained water in the product exuded, the moisture stability decreased, and the sensory score significantly decreased between 16 to 20 days. The spoilage of the product was characterized by a significant water loss, reduction in volume, color change from bright green to dark yellow-brown, and a distinct smell of decaying algae. No coliform bacteria was detected in all products during the storage period. In summary, the shelf life endpoint of the product stored at 25 °C is 16 days, and the shelf life of the product stored at 4 °C is greater than 20 days. Storage at 4 °C can better maintain product quality, extend the shelf life, and effectively maintain the overall color of the product.

Senescence metabolomics of <i>Nicotiana tabacum</i> L. VBI-0 heterotrophic suspension cultures

BACKGROUND: Heterotrophic cell cultures are widely used as a model in plant biology. During a culture cycle the composition of the medium changes: the sucrose and other substrates are depleted, metabolism products are accumulated and the density increases. Finally, arrest of a growth is followed by cell death in a short time. These processes are accompanied with physiological alterations, corresponding to senescence. AIM: To resolve metabolic features of tobacco cells in growing and stationary senescent suspension cultures VBI-0. MATERIALS AND METHODS: Nicotiana tabacum VBI-0 cells were cultured in suspension MS medium supplied with 3% sucrose. Cells were sampled at 7th day, during intensive growth, and at 28th day, when the culture was in the stationary phase. The GC-MS method was used to profile the metabolites. RESULTS: Sucrose depletion in media caused starvation of heterotrophic tobacco cell culture and was associated with a decrease in the accumulation of free amino acids. At the same time, the level of pentoses and complex sugars, including sucrose, increased, while the levels of glucose and fructose were not changed significantly and levels of hexose phosphates decreased. During culture senescence cells showed higher levels of accumulation of malate, pyruvate and some other carboxylates. CONCLUSIONS: The metabolomic data indicate that culture senescence was associated with a drop in amino acids metabolism, a decrease in the activity of the upper part of glycolysis, and the accumulation of complex sugars, pentoses and carboxylates.

Effects of electron beam irradiation on the postharvest quality characteristics of winter jujube (Zizyphus jujuba mill. cv. Dalidongzao)

Winter jujube was irradiated with 0.5 kGy electron beam (EB) and stored at 0 °C for 60 days. The quality of winter jujube was analyzed every 10 days to explore the effect of EB on antioxidant activity of winter jujube. Results indicated that 0.5 kGy EB reduced the decay rate of winter jujube (25.95% the 60th day), maintained the hardness and cell membrane integrity, delayed the changes of total soluble solids (TSS) and ascorbic acid (AA) contents, moreover maintained high contents of total phenols (TPC) and flavonoids. Furthermore, the activities of superoxide dismutase (SOD), peroxidase (POD), catalase (CAT) and ascorbate peroxidase (APX) increased, and the free radical scavenging ability of 2,2-diphenyl-1-picrylhydrazyl (DPPH) radicals and 2,2-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) cation radicals and the ferric ion reducing antioxidant power (FRAP) increased. Research demonstrated the potential of EB in the preservation of winter jujube and provided important information for the application of irradiation technology.

Evaluation of Infected and Non-infected Wounds Healing Activity of Eriosema robustum Hydroethanolic Leaves Extract Ointments in Streptozotocin Induced Diabetic Rats

Untreated diabetic wounds provide an optimal environment for bacterial growth, which, over time, can develop resistance to common antibiotics and ultimately result in amputation. Therefore, it is necessary to search for new sources of antimicrobial molecules with wound healing activity owing to the presence of different secondary metabolites in medicinal plants. Aims: This study was to evaluate the in vivo antibacterial and diabetic wound healing capabilities of 70° hydroethanolic extract of Eriosema robustum leaves on non-infected and infected diabetic wound. Methodology: To do this, obese albino Wistar male rats (200–280 g) were divided into eleven groups and were made diabetic by intraperitoneal injection with a low dose of streptozotocin at 45 mg/kg of body weight. An excision wound with a surface area of 314 mm2 was created on the dorsal area of each animal, except in the uninjured diabetic group (UDG). The 70° hydroethanolic extract was used to prepare 1%, 5%, and 10% ointments, with L-Mesitran serving as the reference ointment. Healing potential was assessed by measuring wound contraction rates and determining serum and tissue hydroxyproline, serum lactate deydrogenase (LDH) and total protein levels (TP). The antibacterial power evaluated in vivo of Eriosema robustum leaves was also assessed by culturing the skin after healing. Results: The results demonstrated a significantly faster healing rate in the non-infected groups (5%,10% and L-Mesitran) compared to the infected groups. The levels of tissue hydroxyproline and total proteins were significantly (p < 0.05) elevated in all treated groups compared to infected and negative controls, unlike serum hydroxyproline levels. LDH levels were significantly (p < 0.05) elevated in both negative control group compared to the treated groups. The culture of different skin samples on previously injured areas on the 20th day of treatment showed no growth of S. aureus on completely healed areas and a low rate in the groups treated during the healing process. Conclusion: 70° Hydroethanolic leaves extract of Eriosema robustum possess in vivoantibacterial activities and diabetic wound healing potential.

Study on the Toxic Effects of Deltamethrin on Hematological Parameters of a Fresh Water Fish, Common Carp (Cyprinus carpio L.)

Fish health is adversely affected by pesticide contamination, which is a significant cause of pollution in freshwater ecosystems. One helpful technique for assessing the general health of different fish species is the analysis of blood parameters. Present study investigated the detrimental effects of deltamethrin on different hematological parameters of a fresh water fish, common carp (Cyprinus carpio). An experiment with a 28-day exposure to 1/20th and 1/10th of the 96-hour LC50 value (0.114µl/L) was conducted in order to investigate the effects of sub-lethal concentrations. On the 7th, 14th, 21st, and 28th day of exposure, several hematological parameters like Total Erythrocyte Count (TEC), Total Leukocyte Count (TLC), Hemoglobin (Hb), Hematocrit/Packed Cell Volume (Hct/PCV), Mean Corpuscular Volume (MCV), Mean Corpuscular Hemoglobin (MCH) and Mean Corpuscular Hemoglobin Concentration (MCHC) were analyzed in comparison to control. After the exposure of different concentrations of deltamethrin significant decline (P*<0.05 and P**<0.01) in Hb, TEC, PCV and MCHC was noted as compared to control. On the contrary TLC, MCV and MCH showed increasing trend after the treatment of pesticide. This study showed that deltamethrin considerably altered the hematological markers of fish, even at sub-lethal concentrations.

Specific Mode Electroacupuncture Stimulation Mediates the Delivery of NGF Across the Hippocampus Blood-Brain Barrier Through p65-VEGFA-TJs to Improve the Cognitive Function of MCAO/R Convalescent Rats.

Cognitive impairment frequently presents as a prevalent consequence following stroke, imposing significant burdens on patients, families, and society. The objective of this study was to assess the effectiveness and underlying mechanism of nerve growth factor (NGF) in treating post-stroke cognitive dysfunction in rats with cerebral ischemia-reperfusion injury (MCAO/R) through delivery into the brain using specific mode electroacupuncture stimulation (SMES). From the 28th day after modeling, the rats were treated with NGF mediated by SMES, and the cognitive function of the rats was observed after treatment. Learning and memory ability were evaluated using behavioral tests. The impact of SMES on blood-brain barrier (BBB) permeability, the underlying mechanism of cognitive enhancement in rats with MCAO/R, including transmission electron microscopy, enzyme-linked immunosorbent assay, immunohistochemistry, immunofluorescence, and TUNEL staining. We reported that SMES demonstrates a safe and efficient ability to open the BBB during the cerebral ischemia repair phase, facilitating the delivery of NGF to the brain by the p65-VEGFA-TJs pathway.

A Clinical Study to Evaluate the Efficacy of Vyoshadi Sakthu on Elevated Lipid Profile

This single-armed open clinical study explores the efficacy of Vyoshadi Sakthu in managing hyperlipidemia, a condition marked by elevated levels of cholesterol and triglycerides, posing substantial health risks such as cardiovascular diseases and obesity. Administered orally with buttermilk, Vyoshadi Sakthu leverages its Lekhana and Medohara properties to target excess fat accumulation. The study involved 20 participants meeting diagnostic criteria, with lipid profiles assessed at baseline and after a 60th day Vyoshadi Sakthu trial. Statistical analysis, employing the paired t-test, revealed significant reductions in serum cholesterol, LDL, and VLDL levels (p < 0.001) post-intervention. These findings suggest a meaningful impact on lipid metabolism attributed to Vyoshadi Sakthu's Lekhana properties. Additionally, the study highlights potential synergies between Vyoshadi Sakthu treatment and lifestyle modifications, including dietary adjustments and regular exercise. This integrated approach may enhance treatment outcomes, emphasizing holistic management strategies for hyperlipidemia and related metabolic disorders. The results contribute to the growing evidence supporting Ayurvedic interventions' therapeutic efficacy in hyperlipidemia management. However, larger controlled studies are required to validate these findings and ensure the long-term safety and efficacy of Vyoshadi Sakthu. This underscores the importance of integrating traditional knowledge systems like Ayurveda into contemporary healthcare practices to address the complexities of metabolic disorders effectively.

Effectiveness of Green Mussel Chitosan Supplement in Feed on the Growth of Vaname Shrimp (Litopenaeus vannamei)

White shrimp (Litopenaeus vannamei) is an important aquaculture commodity with production demand that continues to increase every year. The addition of chitosan from green mussel shells in feed can increase calcium availability, support the moulting process, and increase shrimp growth and survival. This study aims to examine the effectiveness of green mussel chitosan supplements on the growth and survival of vaname shrimp. This research consists of three stages, namely making chitosan nanoparticles from green mussel shells, making feed with various doses of chitosan nanoparticles (0%/A, 10%/B, 15%/C, and 20%/D), and cultivating vaname shrimp. Feed is made by mixing chitosan nanoparticles with commercial feed, then drying and crushing it into small crumbs. White vaname shrimp are kept in concrete ponds for 80 days, with water quality monitoring and regular measurements of growth in weight and length. Data shows that C treatment with a dose of 15% chitosan nanoparticles resulted in the highest growth in weight and length of vanamei shrimp, reaching an average weight of 12.58 grams and a length of 14.07 cm on the 80th day. Treatment C provides optimal growth compared to other treatments because chitosan accelerates the moulting process and exoskeleton mineralization. Water quality during the study, including a temperature of 29.99°C, pH 7.23, and ammonia content of 0.25 ppm, was within the tolerable range for shrimp growth.

Elucidation of synbiotic diet comprising of Lactobacillus plantarum L20 and Sargassum polycystum on gastrointestinal microbiota, tissue structures and AHPND associated dysbiosis susceptibility in black tiger shrimp (Penaeus monodon)

This study investigates the effect of a synbiotic combination comprising of Lactobacillus plantarum L20 and Sargassum polycystum on gastrointestinal microbiota, tissue structures and AHPND infection in black tiger shrimp, P. monodon. The shrimp were randomly distributed into synbiotic (feed that was incorporated with 108 CFU/g of L. plantarum L20 and 2% S. polycystum) and control group (feed that was incorporated with PBS). After 35 days of feeding trial, all groups were subjected to an AHPND-causing V. parahaemolyticus S2–4 immersion challenge at 6 × 105 CFU/mL. The shrimp gastrointestinal tract from both groups were collected from the feeding trial and challenge test and subjected to high throughput 16S rRNA amplicon sequencing and histopathological analysis. Following 35th day of feeding trial, there were growth enhancement in shrimp, but no significant differences (p > 0.05) in alpha and beta diversities in gastrointestinal microbiota for both groups. However, synbiotic group exhibited a differential abundance of beneficial bacteria, including Lactobacillaceae and Lactiplantibacillus, which were not observed in the control group. Following 60 h post-challenge, there was improvement in shrimp survival in synbiotic group, with slightly significant differences (p < 0.05) were observed in alpha diversity and but no significant differences (p > 0.05) were observed in beta diversity. Synbiotic group demonstrated negative correlation between the gastrointestinal microbiota with Vibrionaceae, enriched potential metabolic pathway of energy generation and microbiota proliferation, and protected gastrointestinal tract structure during the study. The findings suggest that synbiotic diet comprising of L. plantarum L20 and S. polycystum improve growth performance, disease resistance and gastrointestinal structure protection through enhancement of few beneficial bacteria group abundance, despite insignificantly changes of gastrointestinal microbiota with control diet in the shrimp.

Ayurvedic Management of Primary Dysmenorrhea with Sapthasara Arka

Dysmenorrhea is one of the most common gynecological conditions that affect the quality of life of women. The incidence of primary dysmenorrhea of sufficient magnitude to cause pain with incapacitation is about 15–25%. Udavartha, one among the Vathika Yonirogas, characterized by painful menstruation and relief of symptoms after blood discharge; can be symptomatically correlated to primary dysmenorrhoea. Sapthasara Kashaya, mentioned in Chikitsa Manjari, is indicated in the Ruja of Yoni, Hrith, Kukshi, and Prushta. The drugs in the Sapthasara Kashaya are Vathakapha samana and Vatanulomana in action. Methodology: Here is a case report of an 18-year-old girl who presented with complaints of severe pain during menstruation, low back ache, nausea, vomiting, and giddiness for the past 2 years. Her USG reports were found to be normal and so were diagnosed as a case of primary dysmenorrhoea. The patient was given 24ml of Sapthasara Arka with the same amount of water orally twice daily, half an hour before food, for 60 days. The assessment was done on 0th, 30th, 60th, and 90th days using the Visual Analogue Scale and the Verbal Descriptive Scale. Result: Signs and symptoms were significantly reduced on the 30th day of treatment and they were absent on 60th day and 90th day of follow-up. Conclusion: In primary dysmenorrhoea, the administration of Sapthasara Arka was found to be effective.

Age-Dependent Effects of Yolkin on Contact Sensitivity and Immune Phenotypes in Juvenile Mice.

Yolkin, an egg yolk immunoregulatory protein, stimulates the humoral but inhibits the cellular immune response in adult mice. The aim of this investigation was to evaluate the effects of yolkin administration on the immune response using a model of juvenile, i.e., 28-day- and 37-day-old, mice. We examined the yolkin influence on the magnitude of the cellular immune response, which was determined as contact sensitivity (CS) to oxazolone (OXA), and the humoral immune response, which was determined as the antibody response to ovalbumin (OVA). Yolkin was administered in drinking water, followed by immunization with OXA or OVA. In parallel, the phenotypic changes in the lymphoid organs were determined following yolkin treatment and prior immunization. The results showed that yolkin had a stimulatory effect on CS in the mice treated with yolkin from the 37th day of life but not from the 28th day of life. In contrast, no regulatory effect of yolkin on antibody production was found in 28-day- and 37-day-old mice. Phenotypic studies revealed significant changes in the content of B cells and T cell subpopulations, including CD4+CD25+Foxp3 regulatory T cells. The association between the effects of yolkin on the magnitude of CS and phenotypic changes in main T- and B-cell compartments, as well the importance of changes in T-regulatory and CD8+ cells in the age categories, are discussed. We conclude that the immunoregulatory effects of yolkin on the generation of CS in mice are age dependent and change from stimulation in juvenile to suppression in adult mice.