International Journal of Computational Engineering ScienceVol. 04, No. 02, pp. 285-288 (2003) BioMEMS and MicrofluidsNo AccessAN INTEGRATED SYSTEM FOR REAL-TIME PCR ANALYSIS BASED ON MICROFLUIDIC BIOCHIPQINGHUI WANG, YIN TAN, and HAIQING GONGQINGHUI WANGBioMEMS Laboratory, School of Mechanical and Production Engineering, Nanyang Technological University, Singapore Search for more papers by this author , YIN TANBioMEMS Laboratory, School of Mechanical and Production Engineering, Nanyang Technological University, Singapore Search for more papers by this author , and HAIQING GONGBioMEMS Laboratory, School of Mechanical and Production Engineering, Nanyang Technological University, Singapore Search for more papers by this author https://doi.org/10.1142/S1465876303001095Cited by:6 PreviousNext AboutSectionsPDF/EPUB ToolsAdd to favoritesDownload CitationsTrack CitationsRecommend to Library ShareShare onFacebookTwitterLinked InRedditEmail AbstractTogether with our efforts of developing low-cost and high throughput DNA analysis chips based on microfluidics technologies, an automated and integrated system has been developed for real-time polymerase chain reaction (PCR) analysis based on the microfluidic PCR array chips. In order to yield rapid and stable thermal cycling in liquid sample contained in biochip wells, efforts have been made on optimizing thermal cycling performance through PID control. Rapid and homogenous PCR thermal cycling has been achieved with the system. In this paper, we present the system instrumentation and its thermal cycling control.Keywords:Instrumentationthermal cyclingPCRbiochip References J. Chenget al., Nucleic Acids Res. 24, 380 (1996). Crossref, Google ScholarY. C. Lin, C. C. Yang and M. Y. Huang, Sens. Actuators B 71, 127 (2000). Crossref, Google Scholar J. El-Ali, I. R. Perch-Nielsen, SU-8 based PCR chip with integrated heaters and thermometer, The 16th European Conference on Solid-State Transducers, Prague, Czech (Sep. 2002) 15-18 . Google ScholarM. H. Lee, I. M. Hsing and I. K. Lao, Anal. Chem. 72, 4242 (2002). Crossref, Google ScholarK. Sunet al., Sens. Actuators B 84, 283 (2002). Crossref, Google ScholarC. F. Chouet al., Microelectronic Engineering 61–62, 921 (2002). Crossref, Google ScholarJ. Khandurina and T. E. McKnight, Anal. Chem. 72, 2995 (2000). Crossref, Google ScholarR. P. Odaet al., Anal. Chem. 70, 4361 (1998). Crossref, Google Scholar FiguresReferencesRelatedDetailsCited By 6Optothermal sample preconcentration and manipulation with temperature gradient focusingM. Akbari, M. Bahrami and D. Sinton12 August 2011 | Microfluidics and Nanofluidics, Vol. 12, No. 1-4Simultaneous detection of Salmonella enterica, Escherichia coli O157:H7, and Listeria monocytogenes using oscillatory-flow multiplex PCRHaiying Wang, Chunsun Zhang and Da Xing23 March 2011 | Microchimica Acta, Vol. 173, No. 3-4Real-time PCR array chip with capillary-driven sample loading and reactor sealing for point-of-care applicationsNaveen Ramalingam, Hao-Bing Liu, Chang-Chun Dai, Yu Jiang and Hui Wang et al.7 May 2009 | Biomedical Microdevices, Vol. 11, No. 5Microfluidic devices harboring unsealed reactors for real-time isothermal helicase-dependent amplificationNaveen Ramalingam, Tong Chee San, Teo Jin Kai, Matthew Yew Mun Mak and Hai-Qing Gong9 January 2009 | Microfluidics and Nanofluidics, Vol. 7, No. 3PCR microfluidic devices for DNA amplificationChunsun Zhang, Jinliang Xu, Wenli Ma and Wenling Zheng1 May 2006 | Biotechnology Advances, Vol. 24, No. 3Feedforward Variable Structural Proportional-Integral-Derivative for Temperature Control of Polymerase Chain ReactionXianbo QIU, Jingqi YUAN and Zhifeng WANG1 Apr 2006 | Chinese Journal of Chemical Engineering, Vol. 14, No. 2 Recommended Vol. 04, No. 02 Metrics History KeywordsInstrumentationthermal cyclingPCRbiochipPDF download