28th Day Research Articles

Persistence and degradation of tembotrione in loamy soil: Effect of various organic amendments, moisture regimes and temperatures

In the present study, persistence and degradation of tembotrione, a triketone herbicide, was studied in loamy soil collected from maize field. Effects of organic amendments, moistures and temperatures on tembotrione dissipation were evaluated. Soil samples were processed according to the modified QuEChERS involving dichloromethane solvent and MgSO4 without PSA. Analysis using LC-MS/MS showed >95% recoveries of tembotrione its two metabolites TCMBA and M5 from fortified soils. Tembotrione residues dissipated with time and 85.55 to 98.53% dissipation was found on 90th day under different treatments. Tembotrione dissipation increased with temperature and moisture content of the soil. Among organic amendments, highest dissipation was observed in vermicompost amended soil. Minimum and maximum half-lives of tembotrione were recorded under 35 °C (15.7 days) and air-dry (33 days) conditions, respectively. Residues of tembotrione declined with time while that of TCMBA increased steadily up to 10-45th day in different treatments and declined thereafter. Residues of M5 were not detected in our experiments. Tembotrione persistence was negatively correlated with the organic carbon (%), moisture regimes, and temperature. A good correlation between soil microbial biomass carbon and degradation was found. A two-way ANOVA indicated significant differences between the treatments at 95% confidence level (p < 0.05).

Study on the Effect of Foliar Fertilizers on the Growth and Yield of V108 Heat-Tolerant Cucumber Variety

This study evaluates the efficacy of various foliar fertilizers on the growth and yield of the heat-tolerant cucumber variety TV108. The experimental design included six different foliar fertilizers and a control, with applications made weekly at a concentration of 1 mL per liter of water. Results showed that all foliar treatments significantly enhanced plant height compared to the control. Among these, Treatment T4 (ATONIK foliar fertilizer) demonstrated the most pronounced effect, achieving a main stem height of 216.57 cm by the 70th day, which was significantly higher than other treatments and the control. Treatment T4 also excelled in increasing stem diameter, with a final measurement of 1.48 cm, and in promoting both male and female flower formation. The fruit set rate was highest in Treatment T4, reaching 28.67% on day 56 and maintaining at 8.67% by day 70. This treatment also proved most effective in reducing the incidence of key diseases and pests, including powdery mildew, white mold, green worms, and yellow beetles. Regarding yield, Treatment T4 produced the highest individual fruit yield (2.32 kg/plant), actual yield (33.8 tons/ha), and theoretical yield (84.91 tons/ha). Additionally, it enhanced fruit quality, as indicated by the largest fruit length (15.22 cm), diameter (4.13 cm), and the highest Brix value (3.50%). These results substantiate that Treatment T4 is the most effective foliar fertilizer for enhancing both growth and quality of heat-tolerant cucumbers under experimental conditions.

Contribution of root and soil properties on soil infiltration capacity of gramineous and leguminous forages during root decomposition

Soil properties are generally altered during root decomposition, which impacts soil water infiltration. Gramineous and leguminous plants present distinct root properties, but it remains unclear whether the root or soil properties are primarily responsible for the soil infiltration capability during root decomposition. To identifythe differences in the contributions of root and soil properties to soil infiltration capability between these two species during root decomposition, a potted plant in situ decomposition experiment was conducted using Bothriochloa. ischaemum (Gramineae) and Lespedeza. davurica (Leguminosae) during three root decomposition times (the 90th, 270th and 450th day). The results revealed that all the root properties (root length, root volume, average root diameter and root biomass) gradually decreased during root decomposition, especially the root length (RL) and root volume (RV) of L. davurica in 0–40 cm soil layer, with reductions of 55.35% and 58.73% at the 450th day compared with the 90th day. The reverse was true for the soil porosity and mean weight diameter of the soil aggregates. Compared to B. ischaemum, L. davurica presented relatively high initial infiltration rate (IIR), steady infiltration rate (SIR), and average infiltration rate (AIR) values at three root decomposition times. A great increase in the soil infiltration rate was observed for both species at the late decomposition stage (450th day), especially for L. davurica, with increases of 38.32%, 267.7%, and 137.1% in IIR, SIR, and AIR. The GLM analysis results revealed that root properties had a greater contribution to the soil infiltration capability. The key factors controlling the soil water infiltration process have been quantified as RL and RV. The present study indicates that leguminous forage presented greater soil infiltration capability during root decomposition. A great decrease in RL and RV within the upper soil layers increased the soil porosity and facilitated the formation of soil aggregates, thereby promoting water flow and ultimately improving the soil infiltration capability.

Toxicological evaluation of combination products containing imidacloprid, moxidectin and pyriproxyfen

The purpose of the research is to investigate subacute cutaneous toxicity in rats, allergenic properties in guinea pigs and tolerance of increased doses of drugs based on imidacloprid, moxidectin and pyriproxyfen by dogs and cats of different age groups.Materials and methods. Toxicological studies (subacute cutaneous toxicity, study of allergenic properties) were conducted in the vivarium of the All-Russian Research Institute of Plant Protection, a branch of the Federal Scientific Center for Experimental Veterinary Medicine of the Russian Academy of Sciences, on male rats and guinea pigs. Experiments on the tolerance of drugs by target animal species (dogs, cats) were conducted in the Podolsk department of the All-Russian Research Institute of Plant Protection. The choice of doses, frequency and methods of administration of the studied drugs were determined in accordance with the Guidelines for Experimental (Preclinical) Study of New Pharmacological Substances. All doses were calculated for the drug taking into account the density (1.08 g/cm3).Results and discussion. When studying the acute cutaneous toxicity of the drug on rats, LD50 exceeded the maximum possible dose of 10 000 mg/kg (hazard class 4 according to GOST 12.1.007-76). In a subacute experiment on rats, it was established that doses of 1000 and 500 mg/kg are ineffective (safe), the threshold and toxic doses could not be established. These drugs, when treating animals 8 times with an interval of 3 days in a three- and five-fold increase in the maximum therapeutic dose during the experimental period, did not have a negative effect on the general condition of the animals, their physiological status and behavior; no statistically significant changes in the morphological and biochemical parameters of the blood, physicochemical parameters of urine were noted in animals from the experimental groups compared to the control. However, in one adult dog from the second experimental group, which was used a five-fold increase in the maximum therapeutic dose of the drug, depression was noted on the 20th day of the experiment. The physiological state of the animal returned to normal within 24 hours voluntarily without the use of symptomatic therapy.

Investigation of the effects of melatonin application on storage in plums (Prunus domestica L.) grown under organic and conventional conditions

This study was carried out to investigate the effects of melatonin applications on postharvest quality changes of organic and conventionally grown plum fruit. Melatonin was applied in 0, 50, and 100 µmol L− 1 for organic and conventional samples. The fruits were stored at + 2.0 °C and 90% relative humidity for 28 days. During the storage period, the color, weight loss, firmness, Soluble solids concentration (SSC), titratable acidity (TA), pH, total antioxidant content, and total phenolics were evaluated at 7-day intervals. While no effect of melatonin applications on weight loss of organically grown plums was observed, it was determined that weight loss decreased as the dose of melatonin increased in conventionally grown plums. The lowest weight loss during storage was determined in conventionally grown plums treated with 100 µmolL− 1 melatonin. It was observed that the firmness values decreased as the storage period increased in both cultivation methods. The firmness decreased as the dose of melatonin application increased in organically grown plums, while the firmness increased as the dose of melatonin application increased in conventional cultivation. Melatonin application did not positively affect SSC, pH, and color values. However, it was determined that the mean TA values decreased as the dose of melatonin increased in both cultivation methods. When the total phenol content of organic and conventional plums was examined, it was determined that melatonin application decreased the number of phenolic compounds. The highest phenolic content was determined in the control samples. The total amount of antioxidants was 1.71 µmol TE g− 1 on the 28th day in the highest (100 µmol L− 1) melatonin-treated conventionally grown plums.

Moringa oleifera and Allium sativum in broiler nutrition: carcass traits, biochemical characterization, and economic analysis

To ensure the productivity and economic viability of broiler chicken, this study was designed to determine the impact of Moringa oleifera leaf powder (MLP) and Allium sativum bulb powder (ABP) utilization on carcass trait, biochemical characterization and economic value of broiler chickens. Two hundred and forty (n = 240), 8-day-old (Cobb 500) broiler chickens were randomly allotted to 5 treatments with 4 replicates (12 birds/replicate) in a completely randomized design (CRD). The birds were randomly allotted into five groups: T1: diet without MLP and ABP, T2: diet with MLP replaced soybean meal (SBM) at 1%, T3: diet with MLP replaced SBM at 3%, T4: diet with MLP replaced SBM at 1% + 0.1% ABP and T5: diet with MLP replaced SBM at 3% + 0.3% ABP respectively. Each formulated diet was fed ad libitum from the 2nd to 8th week of age. Tannins, phenols, saponins, flavonoids, and reducing sugar were present in MLP and ABP. The percentage dressed weight on the 27th day in T2 and T3 were (P < 0.05) higher than in T1, but comparable (P > 0.05) to those in T4 and T5. On the 56th day, the breast weight in T5 was (P < 0.05) higher than in T1 and the rest of the treatments. Most of the microbes that were mainly identified from the ceca of birds fed dietary treatments were Staphylococcus species, and Streptococcus species. Production cost was significantly (P < 0.05) reduced in T3 (₦1, 843.76) than in T1 (₦1, 905.86) and the rest of the treatments. Therefore, it is economically wise to feed broiler chicken with MLP and ABP, but most economical when a fed diet with MLP replaces SBM at 1% and a diet with MLP replaces SBM at 3%.

Simultaneous Production of Biogas and Electricity from Anaerobic Digestion of Pine Needles: Sustainable Energy and Waste Management.

Power scarcity and pollution can be overcome with the use of green energy forms like ethanol, biogas, electricity, hydrogen, etc., especially energy produced from renewable and industrial feedstocks. In hilly areas, pine needles are the most abundant biomass that has a low possibility of valorization due to high lignin content. On the other hand, anaerobic digestion (AD) of lignin and animal waste has low biogas yield due to poor conductivity. This study focuses on the simultaneous production of biogas and electricity through the co-digestion of cow dung and pine needles. The digester was initially established and stabilized in the lab to ensure a continuous supply of inoculum throughout the experiment. The optimization process involved the determination of an ideal cow dung-to-water ratio and selecting the appropriate conductive material that can enhance the energy generation from the feedstock. Afterward, both batch and continuous anaerobic digestion experiments were conducted. The results revealed that the addition of powdered graphite (5 mM), activated charcoal (15 mM), and biochar (25 mM) exhibited maximum voltage of 0.71 ± 0.013 V, 0.56 ± 0.013 V, and 0.49 ± 0.011 V on the 30th, 25th and 20th day of AD, respectively. The batch experiment showed that 5 mM graphite powder enhanced electron transfer in the AD process and generated a voltage of 0.77 ± 0.014 V on the 30th day, indicating an increase of ~1.5-fold as compared to the control (0.56 ± 0.019 V). The results from the continuous AD process showed that the digester with cow dung, pine needle, and a conductive material in combination exhibited the maximum voltage of 0.76 ± 0.012 V on the 21st day of AD, while the digester with cow dung only exhibited a maximum voltage of 0.62 ± 0.015 V on the 22nd day of AD, representing a 1.3-fold increase over the control. Furthermore, the current work used discarded plastic items and electrodes from spent batteries to emphasize waste management and aid in attaining sustainable energy and development goals.

The effect of early administration of antibiotics or feeding a diet containing coccidiostats on the level of their accumulation in liver and the redox status of turkeys

Early administration of antibiotics may worsen the functioning of the turkeys’ antioxidant system. It was also assumed that the longer the time of administration of an antibiotic, e.g. a coccidiostat, the greater the risk of its accumulation in the liver. The study aimed to determine whether early administration of antibiotics or feeding a diet containing coccidiostats causes accumulation in the liver and whether it affects the deterioration of the antioxidant system, and whether preventive vaccinations can intensify it. A total of 3 080 female turkeys were randomly allocated to eight groups. The experiment had a two-factorial design, with four treatments (C, M, E, D) and two groups of birds (vaccinated +, unvaccinated −). The C group did not receive the coccidiostat or antibiotics. Group M was administered monensin at 90 mg/kg feed for 56 days of life. Group E received enrofloxacin at 10 mg/kg BW, and group D received doxycycline at 50 mg/kg BW, added to drinking water, for the first 5 days of life. One-day-old turkeys from groups C+, M+, E+, and D+ were administered live-attenuated vaccines against turkey rhinotracheitis and Newcastle disease by coarse spray; 28-day-old birds were administered a subcutaneously injected inactivated vaccine against Ornithobacterium rhinotracheale. Turkeys from groups C-, M-, E-, and D- were not vaccinated. It was determined that as a result of administration of enrofloxacin or doxycycline until the 5th day of life, biotransformation of these antibiotics occurred in the liver until the 56th day of life of the turkeys, which was confirmed by their lower level than the Maximum Residue Level. Because the concentration of monensin in the liver of turkeys gradually increased with the extension of the time of its administration in the diet, it is probable that discontinuing its addition a day before the slaughter of birds will result in the presence of this coccidiostat in the liver of turkeys. Despite the accumulation of monensin in the liver of turkeys, this coccidiostat did not increase oxidative reactions in the organism of turkeys. Vaccination of turkeys can reduce oxidative reactions and apoptosis in the body. However, the effect of the redox system reaction is different immediately after vaccination, which is due to the mechanism of action of the immune system. If it is necessary to administer an antibiotic in the early rearing period, the effects of doxycycline on the organism’s immunity including antioxidant defence will be less severe than those of enrofloxacin.

Experience with the use of hyaluronic acid solution (Diart) in the treatment of patients with degenerative-dystrophic diseases of the hip joint

Background. Degenerative-dystrophic diseases of the joints are chronic progressive pathological conditions, accompanied by such features as presence of pain syndrome, impaired functional capacity, muscle weakness, deformation and instability of the joints. The high prevalence of degenerative-dystrophic diseases of the hip joint necessitates the search for optimal non-invasive treatment methods. Objectives. This study aims to analyze the clinical results of intra-articular injections of hyaluronic acid solution (Diart) in the treatment of patients with II–III stages of degenerative-dystrophic diseases of the hip joint. Materials and methods. The clinical results of 30 patients with II–III stages of degenerative-dystrophic diseases of the hip joint were analyzed. The group included 11 (36.67%) males and 19 (63.33%) females. The mean age of group was 61.43±7.98 years. All patients underwent a course of drug therapy using hyaluronic acid solution (Diart), which included 3 intra-articular injections of the drug, which were performed with the 7-day interval. Clinical results were evaluated at the initial visit, at 7, 14, 30 and 90 days after the first injection of Diart by objective examination of patients and analysis of subjective data using the WOMAC questionnaire. Statistical analysis was performed using Statistica 13 software package. Results. The high effectiveness of intra-articular injections of hyaluronic acid (Diart) in the treatment of patients with II-III stages of degenerative-dystrophic diseases of the hip joint and the absence of complications associated with intra-articular injection of the drug have been established. Signs of post-injection sy- novitis, discomfort, swelling or other unpleasant sensations weren’t observed in any of the examined patients. Comparing the results established during remote period, on the 90th day of observation, and indicators determined at the initial visit, we proved a decrease in the intensity of the pain syndrome in the hip joint by 30.35%, in stiffness — by 29.57%, an improvement in the indicators characterizing the functional capacity by 21.34 % and the total values of the WOMAC index — by 27.37%, after a treatment course using Diart. Conclusion. The high efficiency of using intra-articular injections of hyaluronic acid solution (Diart) in the treatment of patients with II–III stages of degen- erative-dystrophic diseases of the hip joint has been proven.

Characterization of Root Hair Curling and Nodule Development in Soybean-Rhizobia Symbiosis.

Soybean plants form symbiotic nitrogen-fixing nodules with specific rhizobia bacteria. The root hair is the initial infection site for the symbiotic process before the nodules. Since roots and nodules grow in soil and are hard to perceive, little knowledge is available on the process of soybean root hair deformation and nodule development over time. In this study, adaptive microrhizotrons were used to observe root hairs and to investigate detailed root hair deformation and nodule formation subjected to different rhizobia densities. The result showed that the root hair curling angle increased with the increase of rhizobia density. The largest curling angle reached 268° on the 8th day after inoculation. Root hairs were not always straight, even in the uninfected group with a relatively small angle (<45°). The nodule is an organ developed after root hair curling. It was inoculated from curling root hairs and swelled in the root axis on the 15th day after inoculation, with the color changing from light (15th day) to a little dark brown (35th day). There was an error between observing the diameter and the real diameter; thus, a diameter over 1 mm was converted to the real diameter according to the relationship between the perceived diameter and the real diameter. The diameter of the nodule reached 5 mm on the 45th day. Nodule number and curling number were strongly related to rhizobia density with a correlation coefficient of determination of 0.92 and 0.93, respectively. Thus, root hair curling development could be quantified, and nodule number could be estimated through derived formulation.

Comparative Study of Lactogenic Effect and Milk Mutritional Density of Oral Galactagogues in Female Rabbit.

Hypogalactia and agalactia in lactating mothers are the major causes of child malnutrition, mortality, morbidity, and overall ill health. The development of such treatments requires a well-designed preclinical study with suitable laboratory animals, which needs to be improved. Thus, a suitably designed study with a laboratory animal to analyse galactagogue activity, along with an assessment of the quality and quantity of milk, is required. This study aimed to evaluate the potential of rabbits as an animal model for studying lactogenic activity. The structural homology of prolactin, prolactin, and prolactin in humans, rabbits, and rats was studied using BLAST and PyMol to assess similarity in the lactogenic system. Daily and cumulative milk production and pre-treatment (control) and post-treatment (three drugs) in rabbits were recorded and evaluated by analysing protein, fat, lactose, solid non-fat, and ash values. All parameters were recorded on the 0th day and at the end of weeks 1, 2, and 3. Mammary gland histopathology was performed to evaluate the effects on mammary glands. Homology studies revealed that the sequences of the human and rabbit prolactin genes, receptors, and hormones had a high similarity index. Treatment with Domperidone, Metoclopramide, and Shatavari significantly enhanced milk production by enhancing prolactin secretion; only Shatavari increased milk nutrition. Enlargement of the tubuloalveolar ducts of the mammary glands was observed. Our findings suggest that rabbits are robust, reproducible, ethically superior, and preclinically relevant animals for assessing lactogenic activity.

Characterization of fresh biowastes for biogas production and environmental health in Niger Delta, Nigeria

BackgroundEnvironmental pollution is a public health problem in Niger Delta, Nigeria. Therefore, the aims of the present study were to: identify the major fresh biowastes in Bayelsa State, Niger Delta; quantify the biogas yields from mono-digestion and co-digestion of identified biowastes; determine the first day of biogas production during hydraulic retention time; assess pH variations during anaerobic digestion; and evaluate biogas flame colours.MethodsFifteen communities in Bayelsa State were randomly selected, and on-the-spot assessment and quantification of the biowastes found in each community were carried out daily per week. Mono-digestion of 20 kg of each biowaste and co-digestion of 10 kg of animal waste with 10 kg of plant waste were carried out respectively under anaerobic conditions. Cumulative biogas yield and pH were measured using a pH meter and weighing scale respectively. Biogas flame colours during combustion were visually assessed.ResultsExactly 120.61 metric ton of fresh biowastes was found to be generated per week in Bayelsa State, Niger Delta. Industrial biowastes were the highest [47.6 tonnes, (39.46%)], followed by abattoir biowastes [33 tonnes (27%)], market and roadside sellers biowastes [25.5 tonnes (21.14%)], and farm biowastes [14.51 (12.03%)]. Biogas yields (kg) were: 0, 0, 0, 0, 0, 17, 14, 2, 18, 16, 17, 15 and 1 kg for palm oil mill effluent (POME), orange fruit waste (OFW), pineapple peels (PP), plantain peels, cassava mill effluent (CME), rumen digesta (RD), cow dung, sewage, PP-RD, plantain peels-cow dung, POME-rumen digesta, OFW-cow dung, and CME-sewage respectively. The first day of biogas production for RD, cow dung, sewage, PP-RD, plantain peels-cow dung, POME-RD, OFW-cow dung, and CME-sewage was on the 6th, 32nd. 56th, 1st, 26th, 18th, 25th, and 60th day of hydraulic retention time respectively. A dominant blue flame colour mixed reddish yellow or orange flames were found during biogas combustion. A slight increase in pH was found in all the biodigester media.ConclusionsIn the present study, a variety of biowastes yielding various quantities and qualities of biogas were identified in Bayelsa State, Niger Delta. The study’s findings have provided evidence-based data that might be explored as a road map and catalyst for policy creation against inadequate biowaste management and as sustainable alternatives to the expensive liquefied petroleum gas. The potential of current research study to be scaled up for commercial use is implicated in the present study.

Structural and sensory impact of various emulsifiers in cocoa hazelnut spread formulations.

In this study, the effects of emulsifiers such as lecithin, AMPs, Palsgaard® Oil-Binder and GMS on cocoa hazelnut spread rheology were compared under the same process conditions and formulation. Emulsifiers were added to the formulation separately at rates of 0.3%-0.4%-0.5%. Hardness values in cocoa hazelnut spread were examined at 15-day intervals until the 60th day. In addition, viscosity, rheological analyses, color, spreadability, stability tests, and sensory analyses were performed. In the production of cocoa hazelnut spread, lecithin and AMP have less hardness and lower viscosity, greater fluent consistency, better spreadability, and lower "work of shear" values compared with other emulsifiers. The emulsifier type/ratio difference did not affect the color value statistically. It was determined that the use of Oil-Binder and GMS significantly protected the stability compared with other emulsifiers. During the 60-day storage period, lecithin preserved its hardness properties better than other emulsifiers. When sensory properties were examined, the use of lecithin and AMP in cocoa hazelnut spread samples scored high in brightness, spreadability, mouthfeel, and taste parameters. As a result, lecithin comes to the fore in the use of different types and ratios of emulsifiers in cocoa hazelnut spread production technology.

Correlation of Silage Feeds to Blood Biochemical Parameters in Saanen Goats

This paper presents the results of monitoring blood biochemical parameters of Saanen goats when using some types of silage. The study was conducted on 40 Saanen goats 6-12 months old, with an average weight of 17.5 kg, raised at the Center for Livestock Biotechnology. The goats were completely randomized in blocks into 3 treatments (NT); NT1: pseudo-silage cashew nuts, NT2: silage cassava pulp and NT3: silage corn stalks and leaves and 1 control group (using standard feed at the center). Blood samples were taken by jugular vein puncture of each animal in the morning, before feeding on days 1, 45 and 90 of the experiment. Blood biochemical indices: Glucose, Protein, Albumin, Globulin, BUN, Creatinine, AST, ALT, ALP, electrolyte index (Na, K, Ca, P) were measured by Abaxis Vetscan 2 chemical analyzer. Biochemical parameters of the control group and the 3 treatments 1, 2, 3 on the 90th day were respectively Glucose was 4.56 ± 2.86; 4.59 ± 3.84; 4.38 ± 3.88; 4.42 ± 3.18 mmol/l; Protein was 76.83 ± 8.56; 77.56 ± 6.82; 77.36 ± 7.96; 78.35 ± 8.38 g/l. The results of the evaluation of blood biochemical parameters of Saanen goats were all at normal levels when using silage rations. There was no statistical difference between the experimental and control groups. The results confirmed that the use of supplementary silage in Saanen goat farming did not affect blood biochemical indices. These data are useful for further studies on other ruminants, suggesting the use of silage in goat farming from agricultural by-products.

The effects of zinc citrate, selenium citrate, and germanium citrate on hematological parameters of rabbits under heat stress

Background. The environmental crisis has affected the annual ambient tempera­ture increase, adversely affecting the mammalian body. Due to their lack of sweat glands, Rabbits are more sensitive to heat stress than other animals. The effect of elevated ambient temperatures on the rabbit body leads to violations of blood parame­ters, endocrine regulation, immune and reproductive function, which reduces their productivity and increases animal mortality. Particular attention is now paid to the study of organic compounds of trace elements, which are characterized by high physiological activity, are non-toxic, have a wide range of biological effects, and have a positive impact on reducing the negative effect of elevated ambient temperatures on animals. However, their action depends on the element and its applied quantity. Therefore, the main objective of this study was to investigate the effect of zinc citrate, selenium citrate, and germanium citrate on changes in the number of blood cells in rabbits to mitigate the effects of heat stress. Materials and methods. The studies were conducted on young analog rabbits of the Termon White breed from 35 to 78 days of age. The rabbits were kept indoors at elevated ambient temperatures from 28.9 to 30 °C and relative humidity from 78.1 to 87.4 %. Animals of the control group were kept on the main diet with feeding of standard balanced granulated compound feed and water without restriction. Rabbits of groups I, II, and III of the study groups consumed the same compound feed as in control, but within 24 hours, they received water: group I – zinc citrate – 60 mg Zn/L or 12 mg Zn/kg of body weight; group II – selenium citrate – 300 μg Se/L or 60 μg Se/kg of body weight; group III – germanium citrate – 62.5 μg Ge/L or 12.5 μg Ge/kg of body weight. Using individual drinkers for each animal and placing the animals in different cages allowed us to control the amount of water consumed by each rabbit. Blood for the study was selected for supplementation on the 14th day of the prepara­tory period and the 14th and 29th days of the study period. During the study period, the room temperature was monitored, taking into account the temperature and humidity index. Results. The addition of micronutrient citrates to the diet of rabbits during 29 days of study under heat stress caused haematological changes in indicators compared to the control: the number of erythrocytes in the blood of rabbits of experimental groups I and II increased by 16.4 and 13.6 % and 19.9 and 14.5 % on day 14 and 29, respectively, in group III by 15.3 % on day 14; the haemoglobin content of groups I, II and III increased by 20.8, 21.6 and 19.5 % on day 14 and 11.1, 12.5 and 9.7 % on day 29; haematocrit value of groups I and II increased by 24.1 and 15.7 % and 21.1 and 16.5 % during the study, group III by 18.6% on day 14; the number of leukocytes of groups I, II and III decreased by 13.1 and 8.3 %; 11.2 and 10.4 % and by 11.4 and 9.3 % on days 14 and 29; the number of lymphocytes of groups I, II and III decreased by 25.9, 27.3 and 29.0 % on day 14 and by 20.4, 21.7 and 16.0 % on day 29; the number of monocytes of groups I and II increased by 14.8 and 21.3 % and 17.0 and 18.3 % over 29 days; the number of platelets in animals of group II decreased by 29.4 % on day 29, the average volume of red blood cells increased by 11.6 and 14.6 % on days 14 and 29 of the experiment. Conclusions. Adding micronutrient citrates to the rabbit diet mitigated the effects of heat stress on the body. The effect of these additives on animals resulted in significant changes in the hematological parameters of the rabbits’ blood, of which the best results were observed under the influence of selenium citrate (60 μg Se/kg body weight) and zinc citrate (12 mg Zn/kg body weight): red blood cell count (p &lt;0.05–0.01), leukocyte (p &lt;0.05–0.01), lymphocyte (p &lt;0.05–0.01), monocyte (p &lt;0.05–0.01), haemoglobin content (p &lt;0.01–0.001), haematocrit value (p &lt;0.01), compared to the control. Feeding germanium citrate led to less pronounced changes in these blood parameters.

Intelligent locust bean gum-k-carrageenan nanofibrous mats with Rosa canina petal anthocyanins and chitosan nanoparticles: Preparation, characterization, and application for monitoring of beef meat freshness

The objectives of the present experiment were to add Rosa canina petal extract (RCE, 3.5%) and chitosan nanoparticles (CNP, 1.5%) into the locust bean gum-kappa-carrageenan (LBG-KC) nanofiber mats and to examine their utilization for the freshness tracking of beef meat during the 6-day storage period. The surface morphology analysis of nanofibers using the scanning electron microscope showed that all developed nanofibers were cylindrical, uniformly disordered network structures with smooth surfaces. The tensile strength, elongation at break, water vapor permeability, water solubility, and moisture content of RCE/CNP-loaded nanofiber mats were recorded to be 10.00–31.77 MPa, 16.55%–22.98%, 5.04–9.18 × 10‾5 g mm/m2 h Pa, 3.80%–10.08%, and 2.10%–4.89%, respectively. The LBG-KC + RCE 3.5% and LBG-KC + RCE 3.5% + CNP 1.5% nanofibers were red, dark blue, green, and brown at pH values 1–6, 7–8, 9, and 10–12, respectively. After 4 days of beef meat storage at refrigerated conditions, the total viable count, pH, and total volatile basic nitrogen were 7.24 log CFU/g, 7.11, and 28.89 mg N/100 g, respectively. Moreover, on the 0th day, the pH-sensitive indicators presented a white color and after 4 days of meat storage, the indicator color changed to dark blue, which could indicate beef meat spoilage.

An exploratory single-arm open-label clinical study to evaluate the combined effect of Vamana and Sarasvata Churna in reducing excessive daytime sleepiness

BackgroundExcessive Daytime Sleepiness (EDS) is inferred as persistent sleepiness and often a general lack of energy during the day even after apparently adequate or even prolonged nighttime sleep. The subjects may not be identifying excessive daytime sleepiness but may present as feeling tired, lack of energy etc. They may seem apparently healthy and without any other systemic illness. The prevalence was found to be as high as 18 % in 2012 in United Kingdom. Also, it is a risk factor for diseases like Alzheimer's disease, Parkinson's disease and other neurodegenerative diseases. Conventional management (Modafinil, Sodium Oxybate and Stimulants) addresses only the subjective condition in EDS and is associated with adverse effects compromising the quality of life. In the current health scenario there is a need for alternative interventions to manage this disease. On analyzing this condition through Ayurveda, we could understand it as Nidradhikya (Excessive daytime sleepiness) which is caused due to vitiated Kapha Dosha along with Vriddha Tamoguna (increased Tamoguna) that can be managed by Shodhana (biopurification) followed Shamana (pacification) therapies. ObjectiveThis study was aimed to assess the combined effect of Vamana (therapeutic vomiting) and Sarasvata Churna in reducing Excessive Daytime Sleepiness. Materials and methodsThis was a single-arm exploratory open-label clinical trial. 10 subjects having EDS diagnosed using Modified Epworth Sleepiness Scale (MESS) with a score of 10–24 with proper exclusion were recruited from OPD and IPD, Department of Kāyacikitsa, Amrita School of Ayurveda. All the participants were clinically examined, sought for medical history and further checked biochemically to exclude any other systemic illness that may lead to this condition. Only healthy subjects with quality night sleep (assessed by PSQI) and without any other systemic illness were selected. The treatment protocol adopted was Vamana Karma followed by 30 days of Sarasvata Churna as Shamana Aushadha (medicine for pacification). Assessment was done using MESS on 0th day and after 30 days of Shamana Aushadha Sevana. After completion of treatment, the results were statistically analysed using Wilcoxon Signed Rank Test and final conclusion was drawn. ResultsOn analysis of the symptoms using Modified Epworth Sleepiness Scale, the mean total score reduced from 14.8 to 2.8 with 81.08 % relief in these 10 subjects and a significant p value < 0.05 after Vamana followed by 30 days of Shamana Aushadha Sevana. DiscussionVamana helps in Kapha Nirharana (expelling) which removes the Avarana (occlusion) of Manas seen in Nidradhikya. This results in Hrit Shuddhi (purity of heart) that helps in bringing the equilibrium of Mano Gunas (qualities of mind) (Satvoudaryam Tamojayaha) which forms the line of management. Sarasvata Churna which was administered as Shamana Aushadha possesses Medhya (Nootropic), Buddhi Smriti Dhriti Vardhaka (intellect, memory and patience promoting) attributes and is being indicated in Vicetas. Most of the drugs in Sarasvata Churna are Rasayana (rejuvenation) that imparts Indriya Bala (strengthening of senses) and Priti (contempt) to Manas by Prinana (nourishing) of Rasa Dhatu. ConclusionFrom this study, we could conclude that the combination of Vamana Karma followed by Sarasvata Churna administration is effective in reducing EDS.

INVESTIGATION OF THE EFFECT OF ANNUAL AVERAGE TEMPERATURE AND PRECIPITATION CHANGES ON CORS-TR STATIONS: THE CASE OF KSTM STATION

In this study, the effects of meteorological changes on the point positioning of CORS-TR stations were investigated. For this purpose, KURU, SINP, BOYT, CORU, CANK, CMLD, KRBK, KSTM stations were selected. The KSTM station was taken as unknown and adjusted based on other stations. Seasonal normal values of KSTM station in Kastamonu province covering the years 2016-2020 were examined in terms of temperature and precipitation amount. These values were determined according to the minimum, maximum and average value criteria by using Türkiye State Meteorological Service data. For the calculations, IGS-standardized RINEX data of the stations for 5 years and 12 months between 2016 and 2020 and for 10 days on the 11th and 20th days of each month were used. All calculations were processed with Leica Geo Office v8.x. The calculated coordinates were compared with the current coordinates of CORS-TR at the same epoch and examined according to annual temperature and precipitation. In the analyzes, it was tested by statistical method whether all measurements were compatible. When it was examined whether the temperature changes were statistically significant, it was observed that the test values were calculated according to the temperature changes were below the test distribution limit at 95% confidence interval. When it was examined whether the precipitation changes were statistically significant, it was observed that the test values were calculated according to the precipitation changes were below the test distribution limit at 95% confidence interval.

Sub-acute dermal toxicity of Jatropha curcas Linn. leaf extract coated silver nanoparticles (SNPs) in wistar rats

The application of different nanoparticles is underway in the development of phyto-medicines. Silver nanoparticles (AgNPs) are commonly used as coating agents to treat various bacterial and viral diseases. Their safety and potential toxicity necessitate further research to explore the development of nanoparticles coated herbal drugs. The Azadirachta indica (neem) 5% leaf extract and silver nitrate was used to prepare the silver nanoparticles (SNPs) with the biological reduction (green synthesis) method [1]. The application of silver nanoparticles (SNPs) coated with J. carcus leaf extract (JcLE) on Wistar rats showed no adverse sign of toxicity on hematological parameters except an increase in the neutrophil count and increased in total erythrocyte count (TEC) on 28th day. Similarly, serum biochemical values exhibited an increase in aspartate aminotransferase (AST/SGOT) and alanine transaminase (ALT/SGPT) with JcLE-coated SNPs in treated groups. The histopathology revealed that there was no alteration in skin architecture, however, minimal alteration was observed in the liver and kidney with dilatation of the central vein and sinusoidal space, fatty change in the liver, multifocal necrobiotic changes, cystic degeneration and mild focal glomerular atrophy and hyaline cast in the lumen of tubules of kidney. It indicates the proper absorption and excretion of SNPs from the body. There was no significant effect on hematological parameters in the rats treated with J. curcas leaf extract-coated SNP. However, the serum biochemical analysis revealed a toxic effect on the kidney.

Organ-Specific Metabolomics of Aerial Parts of Melochia corchorifolia for Antibiofilm and Wound Healing Activities.

Melochia corchorifolia is a well-known perennial herb and has been used in traditional medicine for the treatment of a wide number of diseases. However, the phytochemical investigation in the different organs of the M. corchorifolia was poorly understood. In the present study, the organ-specific metabolomic profiling of leaves, stems, and vegetable extract of M. corchorifolia was determined, and their potential antibiofilm activity with wound healing properties was evaluated. The UPLC-ESI-Q-TOF-MSE analysis showed 59 compounds in the leaf, stem, and vegetable extracts of M. corchorifolia. The crystal violet staining assay clearly showed that the extracts of M. corchorifolia have excellent antibiofilm activity against Proteus mirabilis and Salmonella typhi. The extracts of M. corchorifolia also caused the architecture of the bacterial biofilm by inhibiting the adherence to polystyrene and auto-aggregation and subsequently inhibiting the growth and colonization of the biofilm-forming bacteria P. mirabilis and S. typhi. The extracts of M. corchorifolia accelerate the wound healing process in BALB/c mice by completely closing the wound on the 20th day of treatment. Together, the phytochemicals present in the leaf, stem, and vegetable extracts of M. corchorifolia are responsible for potent antibiofilm and wound healing properties and could be used as an excellent remedy for treating chronic wounds and their associated infectious disease.