1000-grain Weight Research Articles

IMPACT OF WATER STRESS CONDITIONS ON ASSOCIATION OF AGRO-MORPHOLOGICAL, PHYSIO-CHEMICAL AND KERNEL QUALITY-RELATED TRAITS IN MAIZE HYBRIDS

Changing climatic conditions, especially high temperatures and droughts are one of the major factors hindering sustainable maize production in major maize-growing areas of the world. The current study was designed to determine the association between different morphological, physiological, biochemical, and kernel quality-related traits in maize hybrids under water-deficient conditions. The experiment material was comprised of seven indigenously developed maize hybrids from Maize and Millets Research Institute, Yusafwala-Sahiwal (YH-5482, YH-5427, YH-5404, YH-1899, FH-949, YH-5577, and YH-5395), one maize hybrid developed by local seed company i.e., Kissan Seed Corporation (SB-9663) and two maize hybrids developed by multinational seed companies i.e., Corteva Seeds Pvt Ltd. (P-1543) and Syngenta Seed Pvt Ltd. (NK-8441). The experiment was executed under a randomized complete block design with four replications. The results revealed that highly significant genetic variations were present among maize hybrids for morphological, physiological, biochemical, and kernel quality traits under water-deficient conditions except for ear length for which variations were found to be non-significant. Moreover, correlation coefficient analysis depicts a strong positive correlation of kernel yield with days to 50% silking, days to 50% tasseling, stomatal conductance, transpiration rate, total chlorophyll contents, 1000-kernel weight, ear length, and plant height. However, kernel yield was found to be negatively correlated with hydrogen peroxide, superoxide dismutase, and proline contents. Similarly, path coefficient analysis showed that days to 50% silking had the highest direct positive effect on kernel yield followed by 1000-grain weight, starch contents percentage, and ear height. Therefore, parental material selection must be done using these yield-associated trades under water-deficient conditions.

Enhancing rice productivity through holistic nutrient management: integrating vermicompost and Azolla for improved agronomic performance and sustainability

A three-year multi-location trial on the farmer’s field was conducted in West Bengal, India to evaluate the impact of integrated nutrient management (INM) on the performance of kharif rice cv. Swarna Sub 1. The experiment employed a randomized block design (RBD) across eleven farmers’ fields with similar soil properties, texture, topography, and historical fertilizer application patterns. Three treatments were tested: T1 – common practice of applying N:P:K @ 55:32:27 kg ha−1, T2 – soil test-based application (STA) of ∼75% recommended N dose through chemical fertilizer + ∼ 25% through Azolla, and T3 – soil test-based application of ∼ 75% recommended N dose through chemical fertilizer + 20% N from vermi-compost + 5% N through Azolla. The result of the experiment revealed that the T3 exhibited significant improvements in plant height, leaf area index (LAI), effective tillers meter−2, number of panicles plant−1, and 1000-grain weight compared to T2 and T1. Among the different nutrient management practices, the T3 recorded maximum rice grain productivity than the T1, and T2, Further, the benefit-cost ratio was noticed significantly higher under T3 (1.29) followed by T2 (1.27) and T1 (1.23). The soil organic carbon (SOC), available nutrient was enhanced by the soil test-based application of ∼75% recommended N dose through chemical fertilizer + 20% N from vermicompost + 5% N through Azolla. Thus, our study indicated that the balanced nutrient application through combined sources (T3) could improve crop growth; and sustain rice productivity, besides enhancing the farm income, and soil different soil properties.

Heterologous synthesis of poly-γ-glutamic acid enhanced drought resistance in maize (Zea mays L.)

Drought stress is the main factor restricting maize yield. Poly-γ-glutamic acid (γ-PGA), as a water-retaining agent and fertilizer synergist, could significantly improve the drought resistance and yield of many crops. However, its high production costs and unclear long-term impact on soil ecology limit its large-scale application. In this study, an environmentally friendly green material γ-PGA was heterologous synthesized in maize for the first time using the synthetic biology method. The genes (PgsA, PgsB, PgsC) participated in γ-PGA synthesis were cloned from Bacillus licheniformis and transformed into maize to produce γ-PGA for the first time. Under drought stress, transgenic maize significantly increased the ear length, ear weight and grain weight by 50 % compared to the control, whereas the yield characteristic of ear weight, grain number per ear, grain weight per ear and 100-grain weight increased by 1.67 %–2.33 %, 3.78 %–13.06 %, 8.41 %–22.06 %, 6.03 %–19.28 %, and 11.85 %–18.36 %, respectively under normal growth conditions. γ-PGA was mainly expressed in the mesophyll cells of maize leaf rosette structure and improved drought resistance and yield by protecting and increasing the expression of genes for the photosynthetic and carbon fixation. This study is an important exploration for maize drought stress molecular breeding and building resource-saving agriculture.

Studies on Estimates of Heritability and Genetic Advance for Certain Quantitative Traits in Fieldpea (Pisum sativum L. var. arvense)

Twenty two field pea genotypes were evaluated for estimation of variability, heritability and genetic advance. The Fieldpea genotypes were sown in, Randomized Block Design with three replications at the experimental farm of department of Genetics and Plant Breeding, Faculty of Agriculture, Kamla Nehru Institute of Physical and Social Sciences Sultanpur 228118 (U.P.) India, during Rabi, season 2022-2023. Phenotypic data were recorded for nine characters viz. days to 50% flowering, days to maturity, plant height, number of branches per plant, number of pods per plant, 100-grain weight, grain yield per plant and biological yield per plant. It was reported that in general the value of phenotypic coefficient of variance (PCV) is slightly higher than the value of genotypic coefficient of variance (GCV) indicating the importance of environment in the phenotypic performance of the genotypes studied. The maximum value of GCV was observed for plant height (31.94), grain yield per plant (20.02). High estimates of heritability in broad sense (h2b) were recorded for plant height (97.28), days to 50% flowering (83.96), 100-grain weight (78.48) and days to maturity (64.83). High estimates of genetic advance as percent of mean were recorded for plant height (64.89), 100-grain weight (25.28) and grain yield per plant (25.15). Thus, the characters (plant height, days to 50% flowering, 100-grain weight etc) having high value of GCV, heritability as well as genetic advance may be exploited in Fieldpea breeding program for further improvement in grain yield.

Seed development-related genes contribute to high yield heterosis in integrated utilization of elite autotetraploid and neo-tetraploid rice.

Autotetraploid rice holds high resistance to abiotic stress and substantial promise for yield increase, but it could not be commercially used because of low fertility. Thus, our team developed neo-tetraploid rice with high fertility and hybrid vigor when crossed with indica autotetraploid rice. Despite these advances, the molecular mechanisms underlying this heterosis remain poorly understood. An elite indica autotetraploid rice line (HD11) was used to cross with neo-tetraploid rice, and 34 hybrids were obtained to evaluate agronomic traits related to yield. WE-CLSM, RNA-seq, and CRISPR/Cas9 were employed to observe endosperm structure and identify candidate genes from two represent hybrids. These hybrids showed high seed setting and an approximately 55% increase in 1000-grain weight, some of which achieved grain yields comparable to those of the diploid rice variety. The endosperm observations indicated that the starch grains in the hybrids were more compact than those in paternal lines. A total of 119 seed heterosis related genes (SHRGs) with different expressions were identified, which might contribute to high 1000-grain weight heterosis in neo-tetraploid hybrids. Among them, 12 genes had been found to regulate grain weight formation, including OsFl3, ONAC023, OsNAC024, ONAC025, ONAC026, RAG2, FLO4, FLO11, OsISA1, OsNF-YB1, NF-YC12, and OsYUC9. Haplotype analyses of these 12 genes revealed the various effects on grain weight among different haplotypes. The hybrids could polymerize more dominant haplotypes of above grain weight regulators than any homozygous cultivar. Moreover, two SHRGs (OsFl3 and SHRG2) mutants displayed a significant reduction in 1000-grain weight and an increase in grain chalkiness, indicating that OsFl3 and SHRG2 positively regulate grain weight. Our research has identified a valuable indica autotetraploid germplasm for generating strong yield heterosis in combination with neo-tetraploid lines and gaining molecular insights into the regulatory processes of heterosis in tetraploid rice.

Molecular and agro-morphological characterization of new barley genotypes in arid environments

BackgroundGenetic diversity, population structure, agro-morphological traits, and molecular characteristics, are crucial for either preserving genetic resources or developing new cultivars. Due to climate change, water availability for agricultural use is progressively diminishing. This study used 100 molecular markers (25 TRAP, 22 SRAP, 23 ISTR, and 30 SSR). Additionally, 15 morphological characteristics were utilized to evaluate the optimal agronomic traits of 12 different barley genotypes under arid conditions.ResultsSubstantial variations, ranging from significant to highly significant, were observed in the 15 agromorphological parameters evaluated among the 12 genotypes. The KSU-B101 barley genotype demonstrated superior performance in five specific traits: spike number per plant, 100-grain weight, spike number per square meter, harvest index, and grain yield. These results indicate its potential for achieving high yields in arid regions.The Sahrawy barley genotype exhibited the highest values across five parameters, namely leaf area, spike weight per plant, spike length, spike weight per square meter, and biological yield, making it a promising candidate for animal feed. The KSU-B105 genotype exhibited early maturity and a high grain count per spike, which reflects its early maturity and ability to produce a high number of grains per spike. This suggests its suitability for both animal feed and human food in arid areas. Based on marker data, the molecular study found that the similarity coefficients between the barley genotypes ranged from 0.48 to 0.80, with an average of 0.64. The dendrogram constructed from these data revealed three distinct clusters with a similarity coefficient of 0.80. Notably, the correlation between the dendrogram and its similarity matrix was high (0.903), indicating its accuracy in depicting the genetic relationships. The combined analysis revealed a moderate correlation between the morphological and molecular analysis, suggesting alignment between the two characterization methods.ConclusionsThe morphological and molecular analyses of the 12 barley genotypes in this study effectively revealed the varied genetic characteristics of their agro-performance in arid conditions. KSU-B101, Sahrawy, and KSU-B105 have emerged as promising candidates for different agricultural applications in arid regions. Further research on these genotypes could reveal their full potential for breeding programs.

Ideal phenotypic characteristics and parental lines for hybrid rice breeding to cope with global dimming

The global food crisis has intensified with climate change, and hybrid rice (Oryza sativa L.) is expected to play a crucial role in ensuring world food security. This study analyzed the parental origins and variety characteristics of 930 hybrid rice varieties bred in Sichuan Province, a typical low-light region and one of the main production areas for hybrid rice, from 2002 to 2022. The results indicate that these varieties were hybridized using 329 direct female and 539 direct male parents. Notably, the female parent Peiai 64 and the male parents Yuehesimiao and Fenghuazhan demonstrated a higher probability of breeding rice varieties with high yield and good quality. An ideal panicle type should possess more grains, a lower 1000-grain weight, a higher seed setting percentage, and greater grain density. It is recommended to increase the utilization of excellent parents in Sichuan Province, such as parents with ideal panicle type to improve resistance to climate change, particularly global dimming.

Trait analysis of short duration boro rice (Oryza sativa L.) varieties in northern region of Bangladesh: Insights from heatmap, correlation and PCA

The role of genetic variation is utmost importance in the selection of a desirable genotype for breeding programs. In the northern region of Bangladesh, the performance of five short duration boro rice varieties (BRRI dhan28, BRRI dhan74, BRRI dhan81, BRRI dhan88, and Bangabandhu dhan100) on yield and yield-contributing traits were evaluated in four locations. Yield attributes tiller hill-1 (TN), panicle hill-1 (PN), filled grain panicle-1 (FG), panicle length (PL), 1000-grain weight (TGW), and harvest index (HI), showed a positive correlation with grain yield (GY). Conversely, spikelet sterility (SSP) and unfilled grains panicle-1 (UFG) showed negative associations with grain yield. Principal component analysis (PCA) revealed that the first three components accounted for 89.60% of variability. The highest positive eigenvalue was observed in DF, DM, BY, HI, PN, SY, TN, PL and GY in PC1, indicating their significant influence on the overall genotype variations. PC2 was primarily driven by plant height (PH) and FG, while PC3 driven by TGW and UFG. The results suggest that grain yield is highly influenced by FG, PH, DM, DF, PN, TN, PL, TGW, SY, BY, and HI. These components highlighted the importance in distinguishing rice genotypes with higher grain yield potential. By considering these traits, BRRI dhan74 have outperformed other varieties, followed by BRRI dhan88 and Bangabandhu dhan100. BRRI dhan74 is most suitable for all four location followed by BRRI dhan88. The traits such as PN, PL, FG, TGW, PH, & HI significantly influence rice yield and should be prioritized in breeding programs aimed at developing high-yielding rice varieties.

ASSESSMENT OF COMBINING ABILITY FOR YIELD AND YIELD RELATED TRAITS IN SPRING WHEAT (TRITICUM AESTIVUM L.)

Grain yield and other related characters were studied in 18 F1 generations along with nine parents (six lines and three testers) which were evaluated for combining ability with three replications in a randomized complete block design (RCBD). The results indicated that among various lines, line 9479 was identified as a good general combiner for the traits, viz., spike length, spikelet’s per spike, and 1000-grain weight, whereas, Line 9486 appeared as a good general combiner for the characters, flag leaf area, plant height, peduncle length, number of tillers per plant and 1000-grain weight. Line 9515 for flag leaf area, plant height, and spike length. Line 9519 for the number of tillers per plant, grain yield per plant, and plant height; line 9520 for peduncle length, number of tillers per plant, grains per spike, grain yield per plant and 9521 was identified as good general combiner for the characters spike length, spikelet’s per spike, number of grains per spike and spike density. Tester Punjab-11 emerged as a good general combiner for the traits; peduncle length, plant height, flag leaf area, and number of tillers per plant, whereas, Tester Ass-11 appeared as a good combiner for the spike length, peduncle length, 1000-grain weight, and flag leaf area. Tester Chakwal-50 identified a good general combiner for the number of tillers per plant, plant height, grains per spike, spikelets per spike, grain yield per plant, 1000-grain weight, and spike density. Out of 18 cross combinations, seven crosses viz. 9479 × Aas-11, 9486 × Aas-11, 9515 × Aas-11, 9519 × Chakwal-50, 9520 × Punjab-11, 9521 × Punjab-11, and 9521 × Aas-11 emerged with significant positive SCA effects for grain yield per plant. Thus, these crosses can be exploited for grain yield per plant by using biparental mating system.

Effect of nitrogen and phosphorus on growth and yield of mungbean

Nutrient management and their balance application are crucial for the high productivity of the crops; nitrogen and phosphorus are the essential nutrients, which are often deficient in the soil and must be supplied to the crops through various sources. The excessive application of these nutrients can lead to increased emissions of greenhouse gases and contribute to eutrophication, water pollution, and many other issues. To determine the optimal application rate of these nutrients, a field experiment was conducted in ANASTU research farm, Kandahar, in 2023 to evaluate the effects of different nitrogen and phosphorus levels on the growth and yield of mungbean (Vigna radiata L.). Treatments comprised three levels of nitrogen (0, 20, and 40 kg ha-1) and four levels of phosphorous (0, 40, 60 and 80 kg P2O5 ha-1). The study was carried out using an RCBD design with three replications. The results showed that 20 kg N ha-1 and 40 kg P2O5 ha-1, together and separately, enhanced growth parameters (Plant height, leaf area, dry matter, branches plant-1), yield attributes (Pods plant-1, grains pod-1, 100-grain weight and pod length) and grain, stover and total yield, significantly. Overall, this study indicates that the mungbean should be fertilized with 20 kg N ha–1 and 40 kg P2O5 ha–1 in Dand district of Kandahar province.

Sunflower grain yield and oil content affected by zinc fertilization and genotype in drought stress conditions

ContextDrought stress is a major environmental factor limiting sunflower (Helianthus annus L.) growth and productivity, worldwide. Although there has been research on the use of zinc (Zn) to alleviate drought stress in sunflower, more has yet to be indicated on how Zn may affect sunflower yield and biochemical properties in drought stress conditions as the objective of the present research. MethodsThe field experiment was a split plot on the basis of a complete randomized block design with 18 treatments and 3 replicates conducted in the province of Fars, Iran, in 2018. The experimental treatments of drought (main plots) including irrigating at 70 (control, S1), 105 (mild, S2) and 140 mm (severe, S3) evaporation from the evaporation pan and the sub plots (factorial arrangement) of sunflower genotypes (Shams and Barzegar) and Zn fertilizer (ZnSO4) at 0, 40 and 70 kgha−1 were tested. Different sunflower, growth, yield and biochemical parameters were determined. ResultsStress significantly affected leaf area index (LAI), tray diameter (TD), grain weight (GW), infertile seeds (I), water use efficiency (WUE) and proline (Pr), and genotype was significant on LAI, TD, number of grains per tray (NGT), GW, WUE, and grain protein (PP). However, Zn significantly increased LAI, PP, GW and WUE by 50, 5.9, 35 and 32 %, respectively. ConclusionZn fertilization can alleviate the unfavourable effects of drought stress on sunflower yield and biochemical properties by improving plant growth (LAI), yield (grain weight), and physiology (water use efficiency and protein percentage), and genotype is a determining factor.

Relative Efficiency of Prilled Urea, Urea Super Granules (USG), and Neem-Coated Urea (NCU) for Growth and Yield of Wheat

The experiment took place at the net house of the Agronomy field laboratory, Department of Agronomy and Agricultural Extension, University of Rajshahi, from November 2019 to March 2020, aiming to determine the relative efficiency of prilled urea, urea super granules (USG), and neem-coated urea (NCU) for wheat growth and yield. Five different nitrogenous fertilizers were included: BARC recommended prilled urea at 0.5 g/pot (T1), urea super granules (USG) at 0.25g/pot (T2), neem-coated urea at 0.25g/pot (T3), urea super granules (USG) at 0.5g/pot (T4), and neem-coated urea at 0.5g/pot (T5). The experiment followed a Randomized Block Design (RBD) with three replications. Results indicated significant effects of nitrogenous fertilizer forms on wheat growth and yield characteristics. Across most parameters, the highest values were observed in T5, utilizing 0.5g of neem-coated urea per pot, including plant height (77.34 cm), number of tillers (6.14), effective tillers per pot (23.04), extrusion length (16.57), spike length (17.25), filled grains per pot (1093.43), 1000-grain weight (42.22 g), grain yield (46.39 g/pot), straw yield (77.81g/pot), and biological yield (124.20g/pot). Based on these findings, we can conclude that applying 0.5 g of neem-coated urea per pot (T5) was the most effective treatment for enhancing wheat growth and yield among the tested options.

Rice seeds biofortification using biogenic ıron oxide nanoparticles synthesized by using Glycyrrhiza glabra: a study on growth and yield ımprovement

Iron, a crucial micronutrient, is an integral element of biotic vitality. The scarcity of iron in the soil creates agronomic challenges and has a detrimental impact on crop vigour and chlorophyll formation. Utilizing iron oxide nanoparticles (IONPs) via nanopriming emerges as an innovative method to enhance agricultural efficiency and crop health. The objective of this study was to synthesize biogenic IONPs from Glycyrrhiza glabra (G. glabra) plant extract using green chemistry and to evaluate their nanopriming effects on rice seed iron levels and growth. The synthesized IONPs were analyzed using UV–Vis spectroscopy, Fourier-transform infrared spectroscopy (FTIR), Scanning electron microscope (SEM), Transmission electron microscopy (TEM), and Energy-dispersive X-ray (EDX) techniques. The UV–Vis peak at 280 nm revealed the formation of IONPs. SEM and TEM showed that the nanoparticles were spherical and had an average diameter of 23.8 nm. Nanopriming resulted in a substantial enhancement in growth, as seen by a 9.25% and 22.8% increase in shoot lengths for the 50 ppm and 100 ppm treatments, respectively. The yield metrics showed a positive correlation with the concentrations of IONPs. The 1000-grain weight and spike length observed a maximum increase of 193.75% and 97.73%, respectively, at the highest concentration of IONPs. The study indicates that G. glabra synthesized IONPs as a nanopriming agent significantly increased rice seeds' growth and iron content. This suggests that there is a relationship between the dosage of IONPs and their potential for improving agricultural biofortification.

Assessing the Influence of Planting Dates on Sustainable Maize Production under Drought Stress Conditions

Water is one of the most precious resources and is essential to agricultural output; the biggest user of water is the agricultural sector. Several societal sectors are impacted by the problem of climate change, including agriculture, water resources, and irrigation water demand. A key element in determining sustainable crop production potential is choosing the right cultivars at the right time of year to plant. The dates on which maize is sown are greatly impacted by high summer temperatures and low spring temperatures. Water stress and the timing of sowing can have a significant impact on maize crop yield and water use efficiency. As a result, figuring out the ideal irrigation volume and sowing dates depending on local conditions is essential. A split plot layout was used to create a randomized complete block design for an experiment with five sowing dates (A, B, C, D, and E) and six hybrids (KWS3376, Xinyu 65, KWS9384, Huamei No. 1, Xinyu 102, and Heyu 187). All sowing dates and hybrids had a significant impact on the yield and yield-contributing features (leaf length, ear diameter, grain number per spike, grain breadth, hundred-grain weight, etc.) of maize crops according to the data analysis. A higher grain yield with yield features, such as ear length, number of grains per ear, and hundred-grain weight, was obtained with early-season sowing. Delayed seeding resulted in a lower crop yield. The seasonally delayed seeding of maize reduces yield and yield characteristics. Xinyu 65 produced the highest yield and yield component values of any hybrid. For improved yield and yield traits in the examined area, the study recommended planting maize hybrid Xinyu 65 early in the growing season.

Comprehensive assessment of salt-alkali tolerance of different soybean varieties at the maturation stage

Soil salinization is a significant global challenge impacting food security and arable land safety. Biological amelioration for the development and utilization of salt-alkali land has gained attention in recent years but the breeding and selection of salt-alkali-tolerant crop varieties remain a challenge. Cultivated soybeans are moderately salt-tolerant crops; however, a precise threshold for salt-alkali conditions has not been determined and varies by genotype. Therefore, this study evaluated the salt-alkali tolerance of 15 different soybean varieties using correlation, principal component, membership function, and cluster analyses. The average soybean indices decreased with increasing salt-alkali stress concentrations. Under severe salt-alkali stress, the coefficients of variation for grain yield and biomass were significantly higher than those under mild salt-alkali stress. Plant height and node number were significantly positively correlated, whereas bottom pod height was significantly negatively correlated with branch number, effective pod number, and grain number per plant. Node and grain numbers per plant were significantly positively correlated. Branch number, effective pod number, grain number per plant, and hundred-grain weight were significantly and positively correlated. Effective pod number was significantly positively correlated with grain number per plant, biomass, and yield. Grain number per plant exhibited a significant negative correlation with hundred-grain weight. Biomass and yield were significantly positively correlated. The salt-alkali tolerance of 15 soybean varieties ranged from strong weak as follows: Cangdou 1438, 0734, 1426, 1418, 1453, 1327, 1846, 1412, 1817, 1301, 1821, 1819, 1815, 1814, and 1850. Salt-alkali-sensitive and salt-alkali-tolerant varieties were successfully identified.

Effect of foliar application frequency and different levels of nano fertilizer on growth and development of coriander (Coriandrum sativum L.)

The aim of this study was investigation of PHARMAX nano fertilizer and its frequency of foliar application effects on morphological characteristics, straw yield, fruit yield, percentage and yield of essential oil of (Coriandrum sativum L.). A factorial experiment was conducted based on a randomized complete block design with 8 treatments and 3 replications. Factors were included frequency of foliar application (tillering stage and two weeks after the first foliar application) and concentration of nano fertilizer (0, 1, 3 and 5 ml/L). Finally, the results were statistically analyzed using MSTAT-C software and the mean data were compared with Duncan test at the statistical level of 5 %. The results showed that a significant effect of number of foliar sprays on plant height, number of umbrellas/plant, number of umbrellas/square meter, number of grain/umbrella, grain yield, straw yield, single plant grain weight, single plant biomass yield, percentage and essential oil yield. In addition, different concentrations of nano fertilizer had a significant effect on most traits except the number of grain/umbrella, 1000-Grain weight and essential oil percentage. The interaction of two factors on all traits except number of umbrellas/plant, number of grain/umbrella and percentage and yield of essential oil was significant. With increasing the concentration of nano fertilizer from 0 to 5 ml/L, the number of umbrellas/square meter, grain yields, straw and essential oil yield, increased 62.5, 74.1, 74.3, 186.8 % respectively. In addition, increasing the frequency of foliar application increased the mentioned traits. According to the results, it seems that the treatment of twice-foliar application and concentration of 5 ml/L nano-fertilizer was the best treatment due to increased yield and other traits.

Maize/soybean intercropping increases nutrient uptake, crop yield and modifies soil physio-chemical characteristics and enzymatic activities in the subtropical humid region based in Southwest China

Intercropping, a widely adopted agricultural practice worldwide, aims to increase crop yield, enhance plant nutrient uptake, and optimize the utilization of natural resources, contributing to sustainable farming practices on a global scale. However, the underlying changes in soil physio-chemical characteristics and enzymatic activities, which contribute to crop yield and nutrient uptake in the intercropping systems are largely unknown. Consequently, a two-year (2021–2022) field experiment was conducted on the maize/soybean intercropping practices with/without nitrogen (N) fertilization (i.e., N0; 0 N kg ha−1 and N1; 225 N kg ha−1 for maize and 100 N kg ha−1 for soybean ) to know whether such cropping system can improve the nutrients uptake and crop yields, soil physio-chemical characteristics, and soil enzymes, which ultimately results in enhanced crop yield. The results revealed that maize intercropping treatments (i.e., N0MI and N1MI) had higher crop yield, biomass dry matter, and 1000-grain weight of maize than mono-cropping treatments (i.e., N0MM, and N1MM). Nonetheless, these parameters were optimized in N1MI treatments in both years. For instance, N1MI produced the maximum grain yield (10,105 and 11,705 kg ha−1), biomass dry matter (13,893 and 14,093 kg ha−1), and 1000-grain weight (420 and 449 g) of maize in the year 2021 and 2022, respectively. Conversely, soybean intercropping treatments (i.e., N0SI and N1SI) reduced such yield parameters for soybean. Also, the land equivalent ratio (LER) and land equivalent ratio for N fertilization (LERN) values were always greater than 1, showing the intercropping system’s benefits in terms of yield and improved resource usage. Moreover, maize intercropping treatments (i.e., N0MI and N1MI) and soybean intercropping treatments (i.e., N0SI and N1SI) significantly (p < 0.05) enhanced the nutrient uptake (i.e., N, P, K, Ca, Fe, and Zn) of maize and soybean, however, these nutrients uptakes were more prominent in N1MI and N1SI treatments of maize and soybean, respectively in both years (2021 and 2022) compared with their mono-cropping treatments. Similarly, maize-soybean intercropping treatments (i.e., N0MSI and N1MSI) significantly (p < 0.05) improved the soil-based N, P, K, NH4, NO3, and soil organic matter, but, reduced the soil pH. Such maize-soybean intercropping treatments also improved the soil enzymatic activities such as protease (PT), sucrose (SC), acid phosphatase (AP), urease (UE), and catalase (CT) activities. This indicates that maize-soybean intercropping could potentially contribute to higher and better crop yield, enhanced plant nutrient uptake, improved soil nutrient pool, physio-chemical characteristics, and related soil enzymatic activities. Thus, preferring intercropping to mono-cropping could be a preferable choice for ecologically viable agricultural development.

Time and Doses of Bacillus Subtilis in Leaf Application of Bean Plants

Common beans P. vulgaris is an important food in the diet of the Brazilian population. Currently the use of microorganisms, such as B. subtillis, has been increasing significantly as an alternative to improve productivity of beans. The objective of this research was to identify the ideal dose of the commercial product (Panta Premium) based on B. subtilis, the best time and doses for leaf application, grain yield and its components and seed quality of bean. The statistical design was randomized blocks arranged in a factorial scheme (3 x 6) with four replicates. Three application times (V3, V4, and R5 stages) and six doses of the inoculant based on B. subtilis - strain UFMT Pant001 (0, 200, 400, 600, 800, and 1000 mL per ha-1) were assessed. The number of pods per plant, number of grains per plant, number of grains per pod, 1000-grain weight, grain yield, seed germination, vigor test, first germination count, and accelerated aging test were evaluated. That the 1000-grain weight was not influenced by the different doses and times of B. subtilis application. Positive linear regression with an increase in the number of pods per plant as the dose of inoculant (mL ha-1) increased when applied at the R5 stage. V3 and V4 stages, there was a linear increase (43,1 e 28,4% respectively) in the number of grains per plant as the dose of B. subtilis increased. At the R5 stage, as seen at the V3 and V4 stages, there is a linear behavior where the number of grains per plant increases as the dose of the inoculant based on B. subtilis increases. the results show that both the dose and the time of application of B. subtilis showed influence on the physiological quality of the seed. The increasing doses of B. subtilis strain UFMT Pant001 increased grain yield compared to the control. The leaf application of Bacillus subtilis strain UFMT Pant001 at stage R5 increased the number of pods and grains per plant and grain yield, regardless of the dose. The leaf application of B. subtilis strain UFMT Pant001 increased the physiological quality of the seeds, attributed to the good fertility of the soil at the site.

Physicochemical Properties and Fine Structure of Starch in Jinong Xiangruan 1 and DGR1 Soft Rice Varieties Cultivated in Different Regions of China

Rice, a staple food for billions around the globe, is cultivated in numerous forms. Among them, soft rice is well known, which is characterized by its tender, creamy consistency and desirable texture. In this study, we examined the physicochemical properties and fine structure of starch from two soft rice varieties, Jinong Xiangruan 1 and DGR1, cultivated in different regions in China (Baodi District, Tianjin City; Liaoning Province; and Fengyang City, Anhui Province). The aim was to understand how amylopectin content (AC) influences rice quality. This research aims to bridge the knowledge gap regarding the role of amylopectin in determining rice’s adhesive consistency and viscosity. Significant regional differences were observed in yield components such as the number of grains per panicle, seed setting rates and 1000-grain weight, with Liaoning generally showing higher performance metrics compared to other regions. Physicochemical analysis highlighted that though glue consistency and taste values showed little regional variation, AC significantly influenced rice hardness and viscosity. Rapid Visco Analyzer (RVA) profile analysis further demonstrated distinct differences in viscosity characteristics, underscoring the regional impacts on starch behavior. Additionally, molecular weight distribution and amylopectin chain length analysis, conducted via SEC-MALLS-RI and ICS ion chromatography, revealed notable differences in starch composition across varieties and locations. The findings suggest that environmental conditions play a crucial role in defining starch characteristics and, consequently, the eating quality of rice. This provides valuable insights for breeding high-quality japonica rice with broad adaptability.

Effects of Different Nutrient Management Methods on Growth and Yield of Barley (Hordeum vulgare L.)

A field experiment was conducted during the Rabi season of the year 2022-2023 at the KVK Farm, Mahendergarh of CCS Haryana Agricultural University, Hisar during Rabi season 2022-23 with the objective to study the effect of different nutrient management practices on growth and yield of barley. The experiment containing eleven treatments viz. Control, 100% RDF (60 kg N + 30 kg P2O5 + 15 kg K2O ha-1, 100% RDF) + Azotobactor + PSB, 50% RDF + Azotobactor + PSB, 50% RDF + 50% RDN through Vermicompost, 50% RDF + 50% RDN through Vermicompost+ Azotobactor + PSB, 75% RDF + Azotobactor + PSB, 75% RDF + 25% RDN through Vermicompost, 75% RDF + 25% RDN through Vermicompost + Azotobactor + PSB, 100% RDN through vermicompost and 100% RDN through vermicompost + Azotobactor + PSB. All the growth and yield parameters increased significantly with75% R.D.F + 25% N through Vermicompost. The growth characters like plant height (cm) and number of tillers/mrl were significantly higher under 75% R.D.F + 25% N through Vermicompost + Azotobactor + PSB as compared to other nutrient combinations. The yield components like spike length (cm), number of grains spike-1, grain yield (kg ha-1) and straw yield (kg ha-1) were significantly higher under 75% R.D.F + 25% N through Vermicompost+ Azotobactor + PSB. Harvest index and 1000-grain weight (g) were not influenced significantly due to different nutrient combinations The nitrogen and protein content in grain were obtained higher with 75% R.D.F + 25% N through Vermicompost + Azotobactor + PSB which was significantly higher than rest of treatments. Thus, it can be concluded that a dose of 75% R.D.F + 25% N through Vermicompost + Azotobactor + PSB may be most suitable nutrient combination for achieving higher growth and yield of barley.