ObjectivesThis study aimed to investigate the effects of intermittent and continuous compressive stress on rat mandibular condyles. DesignNinety rats were randomly divided into 5 groups: non-loading control groups and 1-day intermittent cyclic loading, 3-day intermittent cyclic loading, 7-day intermittent cyclic loading, continuous loading groups. In the loading groups, compressive mechanical stresses were loaded onto the condyles. The rats were euthanized after one, two, or four weeks. Histomorphometric and immunochemical staining of the cartilage and the micro-CT scanning of subchondral bone were investigated. ResultsUnder continuous loading following the first week, cartilage thickness, numbers of chondrocytes, extracellular matrix (Type-II collagen) and proliferation decreased significantly, and there was no apparent repair response after the second and fourth weeks. The cartilage of 1-day and 3-day intermittent cyclic loading groups showed similar pathological changes but better proliferative activity compared with the continuous loading group. The 7-day intermittent loading group had significant recovery after 2 weeks, including the increase of cartilage thickness and the number of chondrocytes, cell swelling and rearranging. However, the remodeling of subchondral bone showed no significant difference between the intermittent and continuous loading stress compared to the control group. ConclusionsUnder moderate mechanical stress, the condylar cartilage actively remodeled, whereas the subchondral bone, as supportive rigid structure, was less sensitive to mechanical pressure. The intermittent compressive stress protects the condylar cartilage from excessive damage, which is different from continuous compressive stress. With longer intervals, the cartilage has the potential capability for recovery as the initial state.