Purpose The purpose of this study is to add new insights into the discussion of how a city’s destination marketing organization (DMO) can apply operand resources to act upon its operant resources to create, market and manage a unique brand over time. Design/methodology/approach This study uses an abductive approach to advance understanding of how to execute a branding strategy that evolves over time while strategically curating its unique and valued resources. This understanding is based on an in-depth review of Louisville’s branding journey, including written documents and interviews with people involved in building the branding strategy. Findings This paper presents a model to help destinations focus on how to create, market and manage the delivery of a branded ecosystem that capitalizes on its resources. This study adds new insights into how DMOs can curate a destination’s resources into an ecosystem that delivers its brand promise over time. Originality/value This study introduces the idea of ecotopes to expand an understanding of tourism ecosystems, presents the concept of a customer journey to depict a destination’s branding strategy, incorporates Barney's resource based theory (Barney and Clark, 2007) into the process of creating a destination’s brand and applies Barnard’s (1938) classic acceptance theory of authority to explain how DMOs can execute a destination’s branding strategy.

Crohn disease is a common inflammatory bowel disease that can affect children, adolescents, and adults. It is diagnosed using a combination of physical examination, blood work, endoscopy, and imaging tests.

PurposeThe purpose of this study is to add new insights into the discussion of how a city’s destination marketing organization (DMO) can apply operant resources to act upon its operant resources to create, market and manage a unique brand over time.Design/methodology/approachThe study uses an abductive approach to advance understanding of how to execute a branding strategy that evolves over time while strategically curating its unique and valued resources. This understanding is based on an in-depth review of Louisville’s branding journey, including written documents and interviews with people involved in building the branding strategy.FindingsThis paper presents a model to help destinations focus on how to create, market and manage the delivery of a branded ecosystem that capitalizes on its resources. The study adds new insights into how DMOs can curate a destination’s resources into an ecosystem that delivers its brand promise over time.Originality/valueThe study introduces the idea of ecotopes to expand an understanding of tourism ecosystems, it presents the concept of a customer journey to depict a destination’s branding strategy, incorporates Barney’s Resource Based Theory (Barney & Clark, 2007) into the process of creating a destination’s brand and applies Barnard’s (1938) classic Acceptance Theory of Authority to explain how DMOs can execute a destination’s branding strategy.

Severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) is one of seven coronaviruses known to infect humans. Different from other concerned coronavirus and influenza viruses, SARS‐CoV‐2 has a higher basic reproduction number and thus transmits more efficiently among hosts. Testing animals for SARS‐CoV‐2 may help decipher virus reservoirs, transmission and pathogenesis. Here, we report the first detection of SARS‐CoV‐2 in three snow leopards (Panthera uncia) in a zoo in Kentucky in 2020, the first year of the pandemic. Sequence analysis revealed that snow leopard SARS‐CoV‐2 strains were non‐variant B.1.2 lineage and closely correlated with human strains. One snow leopard shed SARS‐CoV‐2 in faeces up to 4 weeks. Based on clinical signs and viral shedding periods and levels in the three snow leopards, animal‐to‐animal transmission events could not be excluded. Further testing of SARS‐CoV‐2 in animals is needed.

Simple SummaryHuman-caused climate change is proceeding rapidly and providing challenges to wildlife species, especially those adapted to colder temperatures. We investigated the cortisol response of polar bears to increasing ambient temperatures to improve our knowledge of the physiology of this Arctic species with the goal of informing management in zoos and in the wild. In adult polar bears temperatures above 20 °C were associated with an increase in the hormone cortisol to accommodate increased thermoregulatory demands. This temperature threshold was surprisingly high for an Arctic-adapted species. Zoos can provide sufficient housing options to prevent overheating in polar bears exposed to warmer temperatures but our results are concerning for wild polar bears. The number of days reaching 20 °C in the Arctic has increased significantly over the past 30 years and the associated increase in thermoregulatory costs add to the physiological burden many wild polar bears are already facing with the loss of sea ice hunting opportunities. We recommend that the management of polar bears in the wild and under human care be adapted to reflect the increased cortisol concentrations associated with thermoregulatory challenges in warmer temperatures.Our objective was to identify the upper ambient temperature threshold that triggers an increase in cortisol in response to increased thermoregulatory demands in polar bears. The results reported here include endocrine data collected over two years from 25 polar bears housed in 11 accredited zoological institutions across North America. The effects of ambient temperature, sex, age group (juvenile, adult, elderly), breeding season and humidity on fecal cortisol metabolite (FCM) concentrations (N = 8439 samples) were evaluated using linear mixed models. Ambient temperatures were placed into five different categories: <5 °C, 6–10 °C, 11–15 °C, 16–20 °C, and >20 °C. Ambient temperature and humidity had a significant (p < 0.05) effect on FCM concentrations with significant (p < 0.05) interactions of sex, age and breeding season. Once biotic factors were accounted for, there was a significant (p < 0.05) increase in FCM concentrations associated with ambient temperatures above 20 °C in adult polar bears. The implications of these findings for the management of both zoo and wild polar bears are discussed.

Pathology records of bats submitted to the University of Georgia from managed care settings were reviewed to identify naturally occurring diseases. Fifty-nine cases were evaluated during an 11-year period (2008-2019), including representatives from four families: Pteropodidae (Yinpterochiroptera), Phyllostomidae, Vespertilionidae and Molossidae (Yangochiroptera). Pathology reports were reviewed to determine the primary pathological process resulting in death or the decision to euthanize. Cases were categorized as non-infectious (34/59; 58%), infectious/inflammatory (17/59; 29%) or undetermined due to advanced autolysis (8/59; 14%). Musculoskeletal diseases and reproductive losses were the most frequent pathological processes. Among the infectious processes identified, bacterial infections of the reproductive and haemolymphatic systems were most frequently observed. The first two reports of neoplasia in small flying foxes (Pteropus hypomelanus) are described. Bats under managed care present with a wide range of histopathological lesions. In this cohort, non-infectious disease processes were common.

AbstractThe University of Kentucky (U KY) has owned Robinson Forest (37.460723° N, 83.158598° W) since 1923, conducting experiments crucial to understanding the environmental effects of land management in the region. Part of the management of Robinson Forest has been collection of environmental data, including precipitation quantity, bulk‐deposition chemistry, streamflow, stream‐water chemistry, and air and stream temperature. Over the years, these data have been collected and archived using various technologies and have been mostly inaccessible for research use – unedited and uncompiled, scattered across several spreadsheets and paper records. Through a partnership between the U.S. Geological Survey (USGS) and U KY, daily precipitation data for six stations and stream data from four watersheds in Robinson Forest have been compiled for 1971–2018, checked for transcription errors, and annotated for changes in methodologies. These data are available as a USGS data release at https://doi.org/10.5066/P9FPLG1O. Improved accessibility of this data set provides an important research resource for understanding water quality in minimally effected forests in the region. Preliminary results indicate that these data present a valuable opportunity to evaluate linkages among atmospheric deposition and stream chemistry, the effects of environmental policy, such as the Clean Air Act, and effects from nearby land disturbance in the form of surface mining. Furthermore, these data fill a geographic and physiographic gap in what is available to examine deposition and streamflow patterns over the last 45 years, supplementing those long‐term records of research sites in northern (e.g., Hubbard Brook Experimental Forest), central (e.g., Fernow Experimental Forest) and southern Appalachia (e.g., Coweeta Hydrologic Laboratory). As an oasis in the midst of significant surface mining activity, Robinson Forest presents a unique opportunity to understand environmental conditions characteristic of minimally disturbed forests similar to pre‐mining conditions in the Central Appalachian region.

Pre-exposure prophylaxis (PrEP) for HIV prevention can help reduce racial/ethnic HIV disparities in the USA. However, the benefits of PrEP have not been equally distributed across races. Community-informed, culturally tailored media has the potential to increase PrEP awareness and acceptability among vulnerable African-American populations. More research is needed to identify media preferences around PrEP for these groups in order to optimize effectiveness of health messaging. This study details the development of a community-informed multimedia (print, digital, Internet radio, website, social media) campaign to increase PrEP awareness among African-American young adults (age 18-29years). Eleven focus groups with African-American young adults and a community advisory board informed the intervention. Focus group participants expressed concerns with PrEP safety, efficacy, accessibility, the universality of HIV vulnerability, and representation. Campaign elements were then developed based on this feedback. Future studies should examine the effectiveness of culturally tailored multimedia PrEP campaigns.

Sarcocystis falcatula is a well-known cause of fatal pneumonia in some birds, particularly Old World psittacines. Here we describe fatal sarcosystosis due to S. falcatula in 3 penguins (Family Spheniscidae) under managed care, including one African penguin (Spheniscus demersus), and two Southern rockhopper penguins (Eudyptes chrysocome). Randomly distributed foci of necrosis, inflammatory cell infiltrates, edema, and variable numbers of round to elongated protozoal schizonts were observed in sections of lung. Protozoal organisms exhibited strong immunoreactivity for Sarcocystis sp. antigen by immunohistochemistry. Apicomplexan and Sarcocystis genus-specific PCR assays and sequence analysis confirmed S. falcatula as the etiologic agent. These cases of fatal pneumonia attributed to S. falcatula expand the list of aberrant intermediate avian hosts, with particular implications for penguins.