Fenestrulina malusii (Audouin & Savigny), the type species of Fenestrulina Jullien, is among the most widely reported bryozoans globally. Following the loss of the original type material, presumed Mediterranean in origin based on Savigny's depiction on Sargassum, we designate a neotype from a colony on deep Laminaria blades off Ustica Island (Tyrrhenian Sea). Additionally, seven new species are described from various habitats across the Mediterranean: F. cavernicola sp. nov., from the semi-dark and dark zones of a submarine cave in Lesvos Island (NE Aegean Sea); F. communis sp. nov., from beach-cast plastic near Palermo (NW Sicily); F. foveolata sp. nov., from Ile-Rousse Bank (Corsica); F. granulosa sp. nov., from Posidonia meadows in Dhiaporia (Chios Island, Aegean Sea); F. kalliste sp. nov., from outer-shelf coarse sediments off Calvi (NW Corsica); F. ovata sp. nov., from Trémies submarine cave, near Marseille (Gulf of Lion); and F. variorugosa sp. nov., from Posidonia rootlets off Formica Isle (Egadi Archipelago, near the Sicily Strait). Five of these species are currently known only from their type localities, while F. communis sp. nov. and F. variorugosa sp. nov. occur elsewhere. An additional species with distinct features is described but left in open nomenclature owing to the absence of an ovicell. A comprehensive review of the published images of the 77 species currently assigned to Fenestrulina, with the identification of new diagnostic features, including kenozooids, has led to a revised diagnosis of the genus.

Octocorals, a globally distributed class of Cnidaria, inhabit a wide range of environments, from cold to tropical waters and from shallow to deep-sea ecosystems. In the Red Sea, studies on octocoral diversity have mostly been focused on the Gulf of Aqaba and selected families or genera. While these studies have revealed a remarkable richness and diversity of shallow-water species, mesophotic and deep-sea octocoral research remains limited in the region, in particular along the Saudi Arabian coast. Here, we provide a first comprehensive assessment of this group's genetic diversity across the basin's bathymetric and latitudinal gradients. Following six Red Sea oceanographic expeditions and various biodiversity surveys conducted between 2020 to 2023, we analysed a collection of 728 octocoral specimens sampled along 13 degrees of latitude in the Saudi Arabian Red Sea, from shallow-water reefs to deep-sea habitats. We combined morphological identification and sequencing of mitochondrial barcode markers (mtMutS and COI) to delimit lineages. Our integrated results revealed the occurrence of 26 families and 56 genera in the basin from 3 to 859 m of depth. While the description of new species was beyond the scope of this work, here we provide a reference dataset for octocoral diversity from a biodiversity hotspot, as well as essential insights to inform biodiversity management and planning of conservation measures, particularly relevant for the rapidly developing Saudi Arabian Red Sea coast.

Biological invasions threaten biodiversity worldwide. The American blue crab Callinectes sapidus Rathbun, 1896, among the Mediterranean's most damaging invaders, takes up the challenge to transform this threat into gain. To turn its impact into economic value and guide control efforts, we analysed separately the meat composition and exoskeleton biopolymers of adult crabs from three Moroccan protected Sites of Biological and Ecological Interest: Marchica Lagoon (S1), Moulouya Estuary (S2), and Al Hoceima National Park (S3). Marchica specimens exhibited the highest protein content (21.87 ± 1.15 g 100 g-1, p < 0.001) and an elevated lipid fraction, yielding nutrient-dense meat suitable for premium markets. Moulouya crabs were noted for their taste potential, with a higher concentration of fat (1.73 ± 0.09%) and carbohydrates (0.91 ± 0.1%). Al Hoceima individuals displayed markedly mineralised exoskeletons producing lean and low-fat meat, valued in dietary applications. Exoskeleton organic-to-mineral (OM/MM) ratios and proximate composition revealed three adaptive profiles, opportunistic (S1), acclimatory (S2), and conservative (S3), presumably correlated to local salinity, productivity, and substrate conditions, underscoring the species' phenotypic plasticity. X-ray diffraction confirmed the α-chitin polymorph, while FTIR analysis indicated degrees of deacetylation consistent with high-purity chitosan. These findings support the development of a site-specific circular economy framework and may contribute to the ecological resilience of Morocco's protected coastal areas.

Monitoring stranded cetaceans represents a strategic method to assess their health, conservation status, and ecological role in the marine ecosystem. Efficient stranding networks and standardized protocols are essential to monitor this phenomenon and investigate its causes. This study assesses the evidence of fishery interaction on stranded cetacean carcasses found along the Italian coastline from 1986 to 2023. Evidence assessment and post-mortem investigation methods evolved over three macro-periods, from non-standardized reporting (1986–2014) to an integrated national stranding network (2015–2019), and finally to the creation of a new standardized, evidence-based diagnostic framework under the EU-funded LIFE DELFI project (LIFE18 NAT/IT/000942) (2020–2023). A total of 5355 cases were selected for this analysis. A literature review and evidence of interaction on stranded carcasses supported the categorization of findings, ranging from case history to pathological observations, allowing the assessment of temporal variation, demographic parameters, geographical distribution, and fishing gear identification. Evidence of fishery interaction was found in 12.89% of the cases (690/5355), with an annual average of 18.15 affected animals, and fishery interaction was identified as the likely cause of death in 10.32% of the cases. The most frequently reported species were Stenella coeruleoalba and Tursiops truncatus, showing significant differences in fishery interactions, particularly in relation to sex, age class, and geographical distribution. Adult male Tursiops truncatus exhibited a higher susceptibility to gillnet interaction in the Adriatic Sea. The results of this study emphasize the importance of standardized post-mortem investigations and long-term monitoring to identify risk hotspots, implement species- and region-specific mitigation strategies, and establish threshold values for cetacean conservation.

Control of Allergic Rhinitis and Asthma Test for Children (CARATkids) is the first patient‐reported outcome measure (PROM) designed to assess both allergic rhinitis and asthma simultaneously in children aged 6 to 12 years. CARATkids has been validated in several languages and countries, highlighting the need for a review of its psychometric properties. This study aims to evaluate the measurement properties of CARATkids. This systematic review follows PRISMA and COSMIN guidelines. A systematic search was performed across three databases (Ovid/MEDLINE, Web of Science, and Scopus in October 2023, updated in June 2025). We included studies focused on the development, cultural adaptation, or validation of CARATkids, as well as studies comparing CARATkids with other PROMs. We evaluated the quality of CARATkids development, the methodological quality of primary studies, the overall rating, and the certainty of evidence for each CARATkids measurement property and performed a meta‐analysis of its measurement properties. Our search retrieved 193 results. We included nine studies. CARATkids displayed sufficient content validity. Regarding internal consistency, we found a meta‐analytical Cronbach alpha of 0.81 (95% CI = 0.79; 0.83). CARATkids displayed sufficient reliability (meta‐analytical intraclass correlation coefficient 0.86 [95% CI = 0.61; 0.96]). The minimal clinically important difference was 2.76. Construct validity had sufficient evidence for most correlations, with absolute meta‐analytical Spearman coefficients from 0.37 to 0.71. Responsiveness showed strong correlations between CARATkids and most outcome measurement instruments. These findings support CARATkids as a suitable tool for assessing asthma and allergic rhinitis in children aged 6 to 12 years who present both conditions simultaneously.

Sex ratio is an important population metric in animal conservation, but its study in marine species with low sexual dimorphism is often challenging. This work aimed to apply the principles of skin mark analysis to a cetacean species to test its ability to identify sex differences in markings and build a sex-discrimination model exclusively based on photographic material. The common bottlenose dolphin (Tursiops truncatus) was chosen as the model species, and skin marks and their progression over time were identified and measured across the whole-body surface of individuals on photographs acquired between 2016 and 2023 in the Tiber River Estuary area. Interactions with fisheries were quantified both as the proportion of encounters in presence of trawlers, and as a probability of fishing gear injuries. Non-parametric tests were used to assess sex-specific differences. A generalized linear model with binary response (sex) was built through a stepwise procedure, using mark-based indices as predictors. Relevant differences between sexes emerged in markings, with males presenting more social, aggression-related, and fishing-related marks overall, and a stronger tendency of mark accumulation over time. The estimated model reflected the differences highlighted by the statistical tests and had a high accuracy and sensitivity toward both sexes. These results highlight the feasibility and efficacy of this methodological approach to sex-ratio investigations andidentify differences in anthropic pressures insisting on males and females, with important conservation implications. The exclusive use of photographic material makes this methodology potentially applicable and adaptable to multiple species and environments, aiding the efforts of conservationists worldwide.

Mesophotic habitats in the Mediterranean Sea host rich and diverse benthic assemblages, dominated by invertebrates alongside sciaphilous algae. Recent findings suggest that certain mesophotic bioconstructions built by invertebrates, while classified under the coralligenous definition, differ significantly in their taxonomic composition. This study investigates diversity patterns in megabenthic assemblages associated with algal and invertebrate bioconstructions along the Apulian coast, using α- and β-diversity metrics derived from an image analysis approach. Across 360 analyzed frames, 81 taxa were identified, revealing distinct coverage patterns that separate mesophotic algal assemblages from both coral and oyster bioconstructions. Morphological seabed features and primary bioconstructors played a key role in shaping the associated communities. These findings highlight the substantial differences between upper and deeper megabenthic assemblages and emphasize the ecological importance of mesophotic bioconstructions as biodiversity hotspots, underscoring their role in supporting Mediterranean marine ecosystems and the need for targeted conservation strategies.Supplementary InformationThe online version contains supplementary material available at 10.1038/s41598-025-15386-x.

The precipitation of minerals, in particular carbonates, is a widespread phenomenon in all ecosystems, where it assumes a high relevance both from a geological and biogeochemical standpoint. Most carbonate rocks are of biological origin and made in an aquatic environment. In particular, bioprecipitation of carbonates is believed to have started in the Mesoproterozoic Era, thanks to a process often driven by photosynthetic microorganisms. Nevertheless, an important contribution to carbonate precipitation is also due to the metabolic activity of heterotrophic bacteria, which is not restricted to specific taxonomic groups or to specific environments, making this process a ubiquitous phenomenon. In this framework, the relationship between carbonatogenic microorganisms and other living organisms assumes a particular interest. This study aims to isolate and identify the culturable heterotrophic bacterial component associated with the coenosarc of Corallium rubrum in order to evaluate the occurrence of strains able to precipitate carbonates. In particular, the study was focused on the identification and characterisation of bacterial strains isolated from a coral coenosarc showing a high carbonatogenic capacity under laboratory conditions. Samples of C. rubrum were taken in the coastal waters of three Italian regions. The concentration of the aerobic heterotrophic microflora colonising C. rubrum coenosarc samples spanned from 3 to 6∙106 CFU/cm2. This variation in microbial populations colonising the C. rubrum coenosarc, spanning over 6 orders of magnitude, is not mirrored by a corresponding variability in the colony morphotypes recorded, with the mean being 5.1 (±2.1 sd). Among these bacteria, the carbonatogenic predominant species was Staphylococcus equorum (93% of the isolates), whereas Staphylococcus xylosus and Shewanella sp. accounted only for 3% of isolates each. All these strains showed a remarkable capacity of precipitating calcium carbonate, in the form of calcite crystals organised radially as well crystalised spherulites (S. equorum) or coalescing spherulites (Shewanella sp.). S. xylosus only produced amorphous precipitates of calcium carbonate. All bacterial strains identified were positive both for the production of urease and carbon anhydrase in vitro at 30 °C. It seems that they potentially possess the major biochemical abilities conducive to Ca2+ precipitation, as they showed in vitro. In addition, all our carbonatogenic isolates were able to hydrolyse the phytic acid calcium salt and then were potentially able to induce precipitation of calcium phosphates also through such a mechanism.

Abstract Temporal changes in marine assemblages are well documented for large habitat-formers, while data on inconspicuous groups like hydrozoans are scarce. Long-term shifts in hydrozoan communities were assessed by replicating in 2024 an annual survey originally conducted in 1980 and repeated in 2004 along a depth gradient along the rocky cliff of Portofino Promontory (Ligurian Sea). The species richness declined significantly from 83 (1980) to 72 (2004) and 43 (2024), with the greatest losses in cold-affinity species. Several winter-dominant species, such as Eudendrium glomeratum Picard, 1952, Sertularella crassicaulis (Heller, 1868), and Ectopleura larynx (Ellis and Sollander, 1786), have strongly reduced or disappeared. In contrast, summer species like Eudendrium racemosum (Cavolini, 1785) are now present year-round. The appearance of southern species like Corydendrium parasiticum (Linnaeus, 1767) and Pennaria disticha Goldfuss, 1820, along with reduction in sexual reproduction reflect a community-level response to ocean warming, leading to seasonal and bathymetric homogenization. This 44-year dataset provides a rare long-term benchmark and supports the importance of accurate taxonomic analysis for understanding climate-driven transformations in Mediterranean benthic ecosystems.

Sea level rise (SLR) and increased urbanisation of coastal areas have exacerbated coastal flood threats, making them even more severe in important cultural sites. In this context, the role of hard coastal defences such as promenades and embankments needs to be carefully assessed. Here, a thorough investigation is conducted in Grado, one of the most significant coastal and historical towns in the Friuli Venezia Giulia region of Italy. Grado is located on a barrier island of the homonymous lagoon, the northernmost of the Adriatic Sea, and is prone to flooding from both the sea and the back lagoon. The mean and maximum sea levels from the historical dataset of Venice (1950–2023) were analysed using the Gumbel-type distribution, allowing for the identification of annual extremes based on their respective return periods (RPs). Grado and Trieste sea level datasets (1991–2023) were used to calibrate the statistics of the extremes and to calculate the local component (subsidence) of relative SLR. The research examined the occurrence of annual exceedance of the minimum threshold water level of 110 cm, indicating Grado’s initial notable marine ingression. The study includes a detailed analysis of flood impacts on the urban fabric, categorised into sectors based on the promenade elevation on the lagoon side, the most vulnerable to flooding. Inundated areas were obtained using a high-resolution digital terrain model through a GIS-based technique, assessing both the magnitude and exposure of the urban environment to flood risk due to storm surges, also considering relative SLR projections for 2050 and 2100. Currently, approximately 42% of Grado’s inhabited area is inundated with a sea level threshold value of 151 cm, which occurs during surge episodes with a 30-year RP. By 2100, with an optimistic forecast (SSP1-2.6) of local SLR of around +53 cm, the same threshold will be met with a surge of ca. 100 cm, which occurs once a year. Thus, extreme levels linked with more catastrophic events with current secular RPs will be achieved with a multi-year frequency, inundating more than 60% of the urbanized area. Grado, like Venice, exemplifies trends that may impact other coastal regions and historically significant towns of national importance. As a result, the generated simulations, as well as detailed analyses of urban sectors where coastal flooding may occur, are critical for medium- to long-term urban planning aimed at adopting proper adaptation measures.