Zwitterionic Ion Chromatography Coupled with Ultra-violet Detection for the Quantification of 2-Deoxyguanosine in Human Serum

Abstract

A simple ZIC-HILIC-UV technique was developed and applied for the determination of 2-deoxyguanosine in the human serum. The separa-tion took place in the ZIC1 and ZIC5 self-designed stationary phases, in gradient elution mode with a buffer for acetonitrile acetate and 254 nm UV detection. The 2-deoxyguanosine conduct with varying acetate buffer, ACN and pH values has been tested and the results have established the hydrophilicity of 2-deoxyguanosine. The mechanism of separation is based on the hydrophilic and ion exchange partitioning of the2-deoxyguanosine. We have defined and mentioned the current effect of both ZIC1 and ZIC5 stationary stationary phenomena on chromatographic conditions (sodium acetate buffer concentration, ACN and pH). All existing methods are a useful alternative to the current 2-deoxyguanosine separation methods.