Zingiber Officinale Roscoe Prevents Cellular Senescence of Myoblasts in Culture and Promotes Muscle Regeneration.

Nur Fatin Nabilah Mohd Sahardi,Suzana Makpol,Faizul Jaafar,Mariam Firdhaus Mad Nordin

doi:10.1155/2020/1787342

Abstract

Background Ageing resulted in a progressive loss of muscle mass and strength. Increased oxidative stress in ageing affects the capacity of the myoblast to differentiate leading to impairment of muscle regeneration. Zingiber officinale Roscoe (ginger) has potential benefits in reversing muscle ageing due to its antioxidant property. This study aimed to determine the effect of ginger in the prevention of cellular senescence and promotion of muscle regeneration. Methods Myoblast cells were cultured into young and senescent state before treated with different concentrations of ginger standardised extracts containing different concentrations of 6-gingerol and 6-shogaol. Analysis on cellular morphology and myogenic purity was carried out besides determination of SA-β-galactosidase expression and cell cycle profile. Myoblast differentiation was quantitated by determining the fusion index, maturation index, and myotube size. Results Treatment with ginger extracts resulted in improvement of cellular morphology of senescent myoblasts which resembled the morphology of young myoblasts. Our results also showed that ginger treatment caused a significant reduction in SA-β-galactosidase expression on senescent myoblasts indicating prevention of cellular senescence, while cell cycle analysis showed a significant increase in the percentage of cells in the G0/G1 phase and reduction in the S-phase cells. Increased myoblast regenerative capacity was observed as shown by the increased number of nuclei per myotube, fusion index, and maturation index. Conclusions Ginger extracts exerted their potency in promoting muscle regeneration as indicated by prevention of cellular senescence and promotion of myoblast regenerative capacity.

Highlights

Sarcopenia is one of the age-related diseases which is defined as the progressive loss of muscle mass and strength [1]
Sarcopenia begins approximately at the age of 40 years in which muscle mass is predicted to decline about 8% per decade until the age of 70 years [2]. e progressive loss of muscle mass is characterized by the decrement of regenerative capacity of the skeletal muscle involving myoblast cells which can result in impairment of muscle regeneration and difficulty in movement [3]
The number of cell divisions was calculated as log (N/n)/log 2, where N is the number of cells at the time of passage and n is the number of cells initially plated. e cells were divided into two groups which were young cells with population doubling (PD) 14 and senescent cells with PD 21

Summary

Introduction

Sarcopenia is one of the age-related diseases which is defined as the progressive loss of muscle mass and strength [1]. E progressive loss of muscle mass is characterized by the decrement of regenerative capacity of the skeletal muscle involving myoblast cells which can result in impairment of muscle regeneration and difficulty in movement [3]. The capacity of the satellite cell to regenerate is reduced resulting in decreased muscle mass and strength [3]. Increased oxidative stress in ageing affects the capacity of the myoblast to differentiate leading to impairment of muscle regeneration. Our results showed that ginger treatment caused a significant reduction in SA-β-galactosidase expression on senescent myoblasts indicating prevention of cellular senescence, while cell cycle analysis showed a significant increase in the percentage of cells in the G0/G1 phase and reduction in the S-phase cells. Ginger extracts exerted their potency in promoting muscle regeneration as indicated by prevention of cellular senescence and promotion of myoblast regenerative capacity

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Methods

Conclusion