Zingiber cassumunar Roxb. Essential Oil-Loaded Electrospun Poly(lactic acid)/Poly(ethylene oxide) Fiber Blend Membrane for Antibacterial Wound Dressing Application.

Pattawika Sinsup,Peerada Yingyuad,Decha Dechtrirat,Veerawat Teeranachaideekul,Arthit Makarasen,Pongthep Prajongtat,Supanna Techasakul,Laemthong Chuenchom

doi:10.3390/membranes11090648

Abstract

The essential oil from Zingiber cassumunar Roxb. (Plai) has long been used in Thai herbal remedies to treat inflammation, pains, sprains, and wounds. It was therefore loaded into an electrospun fibrous membrane for use as an analgesic and antibacterial dressing for wound care. The polymer blend between poly(lactic acid) and poly(ethylene oxide) was selected as the material of choice because its wettability can be easily tuned by changing the blend ratio. Increasing the hydrophilicity and water uptake ability of the material while retaining its structural integrity and porosity provides moisture balance and removes excess exudates, thereby promoting wound healing. The effect of the blend ratio on the fiber morphology and wettability was investigated using scanning electron microscopy (SEM) and contact angle measurement, respectively. The structural determination of the prepared membranes was conducted using Fourier-transform infrared spectroscopy (FTIR). The release behavior of (E)-1-(3,4-dimethoxyphenyl) butadiene (DMPBD), a marker molecule with potent anti-inflammatory activity from the fiber blend, showed a controlled release characteristic. The essential oil-loaded electrospun membrane also showed antibacterial activity against S. aureus and E. coli. It also exhibited no toxicity to both human fibroblast and keratinocyte cells, suggesting that the prepared material is suitable for wound dressing application.

Highlights

Zingiber cassumunar Roxb., named Plai in Thailand, is a medicinal plant that has long been used in Thai traditional herbal remedies for pain, sprains, inflammation, wounds, skin diseases, asthma, and rheumatism [1,2,3]
The oil-free poly(lactic acid) (PLA)/polyethylene oxide (PEO) fiber blend membranes were successfully prepared through the electrospinning of the PLA/PEO mixture solutions at the polymer concentrations of 8%, 10%, and 12% w/v
The Plai oil-loaded fiber blend membranes at different PLA/PEO ratios were obtained by electrospinning the polymer blend solutions

Summary

Introduction

Zingiber cassumunar Roxb., named Plai in Thailand, is a medicinal plant that has long been used in Thai traditional herbal remedies for pain, sprains, inflammation, wounds, skin diseases, asthma, and rheumatism [1,2,3]. Due in part to its reported medical uses and pharmacological activities, Zingiber cassumunar Roxb. Compared with typical dressings such as gauzes, lint, bandages, and cotton wool, nanofibers are more attractive, owing to their large specific surface area, high porosity, and excellent pore interconnectivity. These extraordinary characteristics exert several advantages for wound healing, such as assisting cell attachment and proliferation as well as facilitating the permeability of moisture and gas, which are beneficial for cell growth and allowing the absorption of additional exudates containing nutrients for bacterial growth. Nanofiber scaffolds closely mimic the structure of the extracellular matrix (ECM) [15,16,17]

Methods

Results

Conclusion