Abstract

Simple, fast and reliable spectrophotometric methods were developed for determination of Omeprazole in bulk and pharmaceutical dosage forms. The solutions of standard and the sample were prepared in Methanol. The quantitative determination of the drug was carried out using the zero order derivative values measured at 238 nm and the area under the curve method values measured at 300-305 nm (n=2). Calibration graphs constructed at their wavelengths of determination were linear in the concentration range of Omeprazole using 2-10?g/.ml (r=0.9985 and r=0.9959) for zero order and area under the curve spectrophotometric method. All the proposed methods have been extensively validated as per ICH guidelines. There was no significant difference between the performance of the proposed methods regarding the mean values and standard deviations. Developed spectrophotometric methods in this study are simple, accurate, precise and sensitive to assay of Omeprazole in tablets.

Highlights

  • Omeprazole is chemically known as 6methoxy-2-[(4-methoxy-3, 5- dimethylpyridin-2-yl) methylsulfinyl]-1Hbenzimidazole

  • Literature survey reveals that USP 2007 and IP 2007[2][3] report HPLC method for assay of omeprazole

  • Analytical methods reported for the estimation of omeprazole is HPLC[4,5,6,7,8,9], LC-MS[10,11]

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Summary

Introduction

Omeprazole is chemically known as 6methoxy-2-[(4-methoxy-3, 5- dimethylpyridin-2-yl) methylsulfinyl]-1Hbenzimidazole. Literature survey reveals that USP 2007 and IP 2007[2][3] report HPLC method for assay of omeprazole. Analytical methods reported for the estimation of omeprazole is HPLC[4,5,6,7,8,9], LC-MS[10,11]. NoUVspectrophotometric method using Zero Order and Area under Curve (AUC) has been reported for the determination of Omeprazole in bulk and tablets. an attempt has been made to develop new Zero Order and Area under Curve Spectrophotometric method for estimation of Omeprazolein bulk and pharmaceutical formulations with good accuracy simplicity, precision and economy

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