Abstract
Background: Zeolite is a microporous aluminosilicate compound which has been successfully used in tissue engineering. The effects of Zeolite on the morphology and functions of pre-osteoblastic MG-63 cells as new bone enhancer material is still unclear.
 
 Methods: In this vitro experimental study, MTT and Alizarin red staining test were performed on six groups of MG-63 cells which differed in Zeolite (Z) concentration and the presence or absence of Alloplast extract (A). Group A: 0.1μg/mL Z+A, Group B: 0.1μg/mL Z without A, Group C: 0.2μg/mL Z+A, Group D: 0.2μg/mL Z without A, Group E: 0.3μg/mL Z+A, Group F: 0.3μg/mL Z without A. There were also three control groups as positive control, negative control, and Alloplast control based on each related test. The data were analyzed by SPSS 20 via one-way ANOVA and Welch test. (P<0.05).
 
 Results: At 24 hours, results showed that solutions with 0.1μg/mL, 0.2μg/mL, and 0.3μg/mL Zeolite with or without Alloplast had significantly higher proliferation rates than positive control (distilled water) groups without Alloplast (p<0.001). At 72hours time point, the results showed significantly higher proliferation rates in the solutions with 0.1μg/mL, 0.2μg/mL, and 0.3μg/mL Zeolite with or without Alloplast compared to the positive control group without Alloplast (p<0.001).
 
 Conclusions: Zeolite can increase proliferation of MG-63 cells without presence of Alloplast; It seems that combination of Zeolite with Alloplast maybe enhancing proliferation and function of MG-63 cells.
Highlights
The bone has a dynamic structure consisting of a mineralized extracellular matrix, Osteocytes, Osteoclasts, blood vessels and nerves
Purchased MG-63 cells were cultured in complete DMEM (Dulbecco’s Modified Eagle Medium) cultures consist of 10% FBS Gibco, USA), 100IU/mL penicillin (Gibco, USA), and 100mg/ml streptomycin (Gibco, USA); cultured cells were incubated at 5% CO2, 95% humidity, and 37 ̊C temperature till reaching the confluency of 90% at which time cells were detached by trypsin and EDTA and passaged
The results of paired group comparisons at 24 hours showed that solutions with 0.1μg/mL, 0.2μg/mL, and 0.3μg/mL Zeolite with or without Alloplast had significantly higher proliferation rates than positive control groups without Alloplast
Summary
The bone has a dynamic structure consisting of a mineralized extracellular matrix, Osteocytes, Osteoclasts, blood vessels and nerves. Reproducing of a lost or a damaged part of the body to recover the structure and function of the tissues is termed regeneration (Ranganath & Nasim, 2017). The term “Bone regeneration” refers to the reparation of injured or lost bone tissue so that both structural and functional integrity will be provided (Gao et al, 2014). Results: At 24 hours, results showed that solutions with 0.1μg/mL, 0.2μg/mL, and 0.3μg/mL Zeolite with or without Alloplast had significantly higher proliferation rates than positive control (distilled water) groups without Alloplast (p
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