Abstract
Zein is a protein found in the endosperm of corn kernels. A variety of food-related applications have been explored using zein. While zein does not have intrinsic antimicrobial properties, it is often explored as a carrier or thin film for known antimicrobials. In this study, the antimicrobial activity of zein under UV-A light exposure is presented. Polypropylene was modified through reactive blending to harbor zein, forming a stable composite exhibiting antimicrobial against E. coli K12 under UV-A light exposure. Fluorometric techniques suggested that this mechanism is driven by generation of reactive oxygen species. The zein-modified polypropylene was able to be reused for 10 cycles without detrimental effects, providing ~90% reduction in the microbial population across the 10 cycles. Its stability was confirmed through spectroscopic and microscopic techniques, that showed marginal effects on its chemical and physical integrity, as well as lack of fouling and cell attachment. In addition, electron microscopy showed visible damage in the morphology of the cells exposed to it.
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