Abstract
This study evaluated physicochemical properties, cytotoxicity and bioactivity of MTA Angelus (MTA), calcium silicate-based cement (CSC) and CSC with 30% Ytterbium oxide (CSC/Yb2O3). Setting time was evaluated using Gilmore needles. Compressive strength was evaluated in a mechanical machine. Radiopacity was evaluated using radiographs of materials and an aluminum scale. Solubility was evaluated after immersion in water. Cell viability was evaluated by means of MTT assay and neutral red staining, and the mineralization activity by using alkaline phosphatase activity and Alizarin Red staining. The data were submitted to ANOVA, Tukey and Bonferroni tests (5% significance). The bioactive potential was evaluated by scanning electron microscopy. The materials presented similar setting time. MTA showed the lowest compressive strength. MTA and CSC/Yb2O3 presented similar radiopacity. CSC/Yb2O3 showed low solubility. Saos-2 cell viability tests showed no cytotoxic effect, except to 1:1 dilution in NR assay which had lower cell viability when compared to the control. ALP at 1 and 7 days was similar to the control. MTA and CSC had greater ALP activity at 3 days when compared to control. All the materials present higher mineralized nodules when compared with the control. SEM analysis showed structures suggesting the presence of calcium phosphate on the surface of materials demonstrating bioactivity. Ytterbium oxide proved to be a properly radiopacifying agent for calcium silicate-based cement since it did not affected the physicochemical and biological properties besides preserving the bioactive potential of this material.
Highlights
Mineral Trioxide Aggregate (MTA) is a biomaterial composed of Portland cement (PC) [1] and bismuth oxide as radiopacifier [2]
calcium silicate-based cement (CSC) was evaluated in pure form or prepared in association with the 30% Yb2O3 (% by weight), which was mixed with distilled water using a powder/liquid ratio determined by previous tests to obtain consistency of retrofilling material and proper radiopacity
The cell viability using MTT assay showed that MTA, CSC
Summary
Mineral Trioxide Aggregate (MTA) is a biomaterial composed of Portland cement (PC) [1] and bismuth oxide as radiopacifier [2]. PC is calcium silicate-based cement (CSC) that contains other chemical compounds such as tricalcium aluminate and presents physicochemical, mechanical and biological properties similar to MTA [2,3]. Calcium silicate cements might be suitable for biomedical applications as biomaterial for bone/dental repair [4]. Bismuth oxide (Bi2O3) reduces calcium hydroxide release, increases solubility, and causes deterioration in dimensional stability of MTA [5]. Lower cell viability was demonstrated for CSC with Bi2O3 in comparison with zirconium oxide and calcium tungstate [6]. New radiopacifiers have been studied as alternatives to Bi2O3
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
