Abstract
In order to determine which cell type or types synthesized serum proteins, yolk sacs from 4-day chick embryos were manually separated into ectoderm, mesoderm, and endoderm and incubated in the presence of radioactive valine. Analysis of the incubation media by polyacrylamide gel electrophoresis as well as by immunoprecipitation showed that all serum proteins were synthesized exclusively by the cells of the endoderm. These included transferrin and three embryo-specific serum proteins: α-globulin a, α-globulin b, and prealbumin.
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