Abstract
Foot and mouth disease (FMD) is one the most contagious diseases in cloven-hoofed animals and has a great potential for causing severe economic loss in susceptible animals. In the fight against FMD disease, inactive FMD vaccines are widely used. This study was carried out for the detection of suitable BHK-21 cell lines in order to get virus suspensions containing excess amounts of 146S FMD virus serotypes O, A and Asia-1, for FMD vaccine production. For this purpose, in addition to the BHK-21 An30 cell culture currently used in FMD vaccine production, BHK-21 An73 cell lines were passaged 20 times. In every 5 passages, infective titers and 146S virus particle quantities of FMD virus serotypes O, A and Asia-1 were measured. Additionally, karyotype analysis was performed on both cells at the 20th passage levels and the tests revealed that there was no significant change in the morphological features and chromosomal structures of the cells.
 It was determined that the infective titer and 146S virus particle quantities of serotypes O, A and Asia-1 of FMD virus were very high in the BHK-21 An73 cell line than they are available in the BHK-21 An30 cell line. The increases of 146S virus particle quantities of serotypes O, A and Asia-1 of FMD virus were statistically significant (p
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