Abstract
BackgroundNormal development of chloroplast is vitally important to plants, but its biological mechanism is still far from fully being understood, especially in rice.ResultsIn this study, a novel yellow-green leaf mutant, ygl138, derived from Nipponbare (Oryza sativa L. ssp. japonica) treated by ethyl methanesulfonate (EMS), was isolated. The mutant exhibited a distinct yellow-green leaf phenotype throughout development, reduced chlorophyll level, and arrested chloroplast development. The phenotype of the ygl138 mutant was caused by a single nuclear gene, which was tentatively designed as YGL138(t). The YGL138(t) locus was mapped to chromosome 11 and isolated into a confined region of 91.8 kb by map-based cloning. Sequencing analysis revealed that, Os11g05552, which was predicted to encode a signal recognition particle 54 kDa (SRP54) protein and act as a chloroplast precursor, had 18 bp nucleotides deletion in the coding region of ygl138 and led to a frameshift. Furthermore, the identity of Os11g05552 was verified by transgenic complementation.ConclusionsThese results are very valuable for further study on YGL138(t) gene and illuminating the mechanism of SRP54 protein involving in chloroplast development of rice.Electronic supplementary materialThe online version of this article (doi:10.1186/1939-8433-6-7) contains supplementary material, which is available to authorized users.
Highlights
Normal development of chloroplast is vitally important to plants, but its biological mechanism is still far from fully being understood, especially in rice
The ygl138 mutant has reduced chlorophyll accumulation and arrested chloroplast development The ygl138 mutant was derived from japonica rice cv Nipponbare treated by ethyl methanesulfonate (EMS), which exhibited a distinct yellow-green leaf phenotype throughout development (Figure 1)
The separate ratio was about 2.88:1, which accorded with 3:1 (χ2 =1.0 < χ2 0.05, 1 = 3.84). These results indicated that the yellow-green leaf phenotype of ygl138 was controlled by a single nuclear gene, which was tentatively designed as YGL138(t)
Summary
Normal development of chloroplast is vitally important to plants, but its biological mechanism is still far from fully being understood, especially in rice. The mechanism of leaf-color mutation is very complicated. Large number of such mutants had been identified in higher plants, such as rice (Hu et al 1981), soybean (Ghirardi and Melis 1988), wheat (Falbel et al 1996), Arabidopsis (Carol et al.1999), maize (Pasini et al 2005), and barley (Liu et al 2008). There had been major advancements in understanding genetic mechanism of leaf-color mutation, with many related genes being identified. Genes for all 15 steps in the chlorophyll biosynthetic have been identified in Arabidopsis. Analysis of the complete genome of Arabidopsis indicated that it has 15 enzymes encoded by 27 genes for chlorophyll biosynthesis from glutamyl-tRNA to Chl b (Nagata et al 2005)
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