Abstract
Yeast surface display (YSD) is a “whole-cell” platform used for the heterologous expression of proteins immobilized on the yeast’s cell surface. YSD combines the advantages eukaryotic systems offer such as post-translational modifications, correct folding and glycosylation of proteins, with ease of cell culturing and genetic manipulation, and allows of protein immobilization and recovery. Additionally, proteins displayed on the surface of yeast cells may show enhanced stability against changes in temperature, pH, organic solvents, and proteases. This platform has been used to study protein-protein interactions, antibody design and protein engineering. Other applications for YSD include library screening, whole-proteome studies, bioremediation, vaccine and antibiotics development, production of biosensors, ethanol production and biocatalysis. YSD is a promising technology that is not yet optimized for biotechnological applications. This mini review is focused on recent strategies to improve the efficiency and selection of displayed proteins. YSD is presented as a cutting-edge technology for the vectorial expression of proteins and peptides. Finally, recent biotechnological applications are summarized. The different approaches described herein could allow for a better strategy cascade for increasing protein/peptide interaction and production.
Highlights
Reviewed by: Roberta Bussons Rodrigues Valério, Federal University of Ceara, Brazil Andre Luiz Barros De Oliveira, Federal University of Ceara, Brazil Tiago Albuquerque, Federal University of Ceara, Brazil Alessandro Angelini, Ca’ Foscari University of Venice, Italy Dae-Hyuk Kweon, Sungkyunkwan University, South Korea
Cell-surface display allows the expression of target peptides or proteins on the cell surface of bacteria, yeast, insect, or mammalian cells through the connection of a protein of interest (POI) fused to an anchor protein
The high content of polysaccharides allows multiple interactions with the proteins embedded in the cell wall that can have a positive impact on the properties of the POI negative effects have been reported depending on the yeast strain used for display (Crowe et al, 1988; Allison et al, 2000; Li et al, 2010)
Summary
Reviewed by: Roberta Bussons Rodrigues Valério, Federal University of Ceara, Brazil Andre Luiz Barros De Oliveira, Federal University of Ceara, Brazil Tiago Albuquerque, Federal University of Ceara, Brazil Alessandro Angelini, Ca’ Foscari University of Venice, Italy Dae-Hyuk Kweon, Sungkyunkwan University, South Korea. Yeast surface display (YSD) is a “whole-cell” platform used for the heterologous expression of proteins immobilized on the yeast’s cell surface.
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