Xylooligosaccharides production by commercial enzyme mixture from agricultural wastes and their prebiotic and antioxidant potential

Abstract

Advancement in industrial biotechnology offers potential opportunities for economic utilization of agro industrial biomass for the production of value-added products. Xylooligosaccharides (XOS) are non-digestible food ingredients with prebiotic properties for selectively promoting the growth of probiotics providing many health benefits and several applications on food and pharmaceutical industry. The present study deal with enzymatic production of XOS from xylan extracted from different agroindustrial wastes, namely sugarcane straw (SS) and coffee husk (CH) using an optimized enzymatic mixture with endo-xylanase (GH11), α-l-arabinofuranosidase (GH51) and Feruloyl Esterase (CE1). The XOS profile concentration was quantified by HPAEC-PAD using standard (X2-X6) from Megazyme®. The efficient enzymatic mixture achieved a high total XOS concentration using SS xylan (10.23 ± 0.56 g/L) and CH xylan (8.45 ± 0.65 g/L). Three of four probiotic cultures of Lactobacillus and Bifidobacterium tested were able to utilize XOS produced from agricultural wastes and showed remarkable growth in the media containing XOS, consuming preferentially the X2 and X3 fractions as the sole source of carbon. The XOS produced also exhibited a considerable resistance to hydrolysis of digestive enzymes, as well as an concentration dependent anti-oxidant activity achieving until 78% in a XOS concentration of 2 g. L−1. Thus, the results showed that XOS produced from these agricultural residues have great prebiotic potential and good antioxidant activity; therefore, it can be used in food-related applications as functional ingredients.