Enzymatic Production of Xylooligosaccharides from Alkali-Solubilized Arabinoxylan from Sugarcane Straw and Coffee Husk

Abstract

Enzymatic hydrolysis of lignocellulosic biomass has opened new perspectives for the production of biofuels and functional food ingredients, such as prebiotic xylooligosaccharides (XOS). This study aimed to improve the arabinoxylan extraction process from sugarcane straw (SS) and coffee husk (CH) to produce short-chain XOS by enzymatic hydrolysis using ideal enzyme mixture formulations. The improvement of arabinoxylan extraction process provided the highest extraction yield of about 40.35% and arabinoxylan extraction efficiency around 82.32% with insignificant lignin content values. In a second step, a central composite rotatable design was used to develop an enzyme mixture for XOS production from SS and CH arabinoxylan. Three commercial enzymes were tested, endoxylanase (GH11), α-l-arabinofuranosidase (GH51), and feruloyl esterase (CE1). An efficient enzyme mixture was developed, affording high XOS concentrations from SS arabinoxylan around 10.23 g L−1 and CH arabinoxylan 8.45 g L−1.