Abstract
Paraburkholderia sacchari revealed an outstanding ability to metabolize D-xylose to xylonic acid. The work described here aimed at finding the operational conditions to promote the production of xylonic acid in bioreactors. Fed-batch assays using xylose as the sole substrate for growth and production attained a xylonic acid concentration of 150 g L−1, corresponding to a productivity of 1.5 g L−1 h−1 and a yield of xylonic acid over xylose (YP/S) of 0.63 g XylAc/gXyl. When glucose and xylose were used sequentially for growth and production respectively, xylonic acid concentrations in the range 370−390 g L−1 were observed, resulting in a volumetric productivity of 6.4–7.7 g L−1 h−1 and a yield of 1.1 gXylAc/gXyl, matching the theoretical yield. These very competitive results were achieved using a simple process set-up.
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