Production of xylonic acid by Klebsiella pneumoniae.

Abstract

The glucose oxidation pathway is important for glucose catabolism in Klebsiella pneumoniae. Gluconic acid and 2-ketogluconic acid are intermediates of this pathway, and the production of these two chemicals has been developed in K. pneumoniae mutants. Catalysis characteristic research in this study has shown that xylose is a suitable substrate of the glucose dehydrogenase of this pathway. Here, using xylose as substrate, xylonic acid was accumulated in the broth of K. pneumoniae culture, and this process was dependent upon acidic conditions. Using a mixture of glucose and xylose as substrates, a mixture of xylonic acid and gluconic acid was produced by the Δgad mutant of K. pneumoniae; gluconic acid was synthesized early, and xylonic acid synthesis began after most glucose was consumed. Using the hydrolysate of bamboo as substrate, mixture of 33g/L gluconic acid and 14g/L xylonic acid were produced by K. pneumoniae Δgad. In fed-batch fermentation, 103g/L xylonic acid was produced after 79h culture, with a conversion ratio of 1.11g/g. This is the first report of xylonic acid produced by K. pneumoniae. Production of xylonic acid and gluconic acid using bamboo hydrolysate is a novel approach for biomass utilization.