Abstract
Objectives: To develop and validate a quantitative analysis method of total flavonoids in the leaves of Cleistocalyx operculatus using UV-Vis method. Subjects and methods: Leaves of Cleistocalyx operculatus were collected in Thua Thien Hue province in August 2021. Determining total flavonoids using a colorimetric method based on the reaction between flavonoids and AlCl3 reagent and validating this procedure according to AOAC guidelines. Results: The quantitative analysis was performed by reaction between total flavonoids and AlCl3 reagent with a detective wavelength of 430 nm. Intra-day and inter- day precision tests showed the RSD < 2% and desirable accuracy (recovery 99.2 - 102.93%). The total flavonoid content of the leaves is 8.34 ± 0.12 mg quercetin equivalent/g dry weight of plant material. Conclusion: The quantitative analysis method of total flavonoids from Cleistocalyx operculatus leaves using the colorimetric method was validated. * Keywords: Cleistocalyx operculatus; Total flavonoids; UV-Vis.
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