Abstract
XV IMBG all-Ukrainian Conference of Young Scientists with international participation
Highlights
DNA integrity of spermatozoa is crucial for oocyte fertilization and normal embryo development
It was found that immobilization of landomycin A on a poly-2-oxazoline nanocarrier leads to an increase in the therapeutic potential of this antitumor antibiotic toward the mice with melanoma B16F10/wt
According to the obtained results, we can state that seedlings of variety Sonechko of H. annuus are susceptible for transformation with A. tumefaciens, and it is possible in the future to transform seedlings of H. annuus with genetic vectors of A. tumefaciens, which will carry the reporter genes and the target genes
Summary
Due to the active and long-term using in the agro-industrial complex of organochlorine pesticides, which include hexachlorobenzene (HCB), the soil condition has undergone negative changes. The aim of the study was to establish the taxonomy position of two strains of soil bacteria isolated from the landfill of organochlorine pesticides by sequencing the 16S rRNA gene. To establish the affinity of the 16S rRNA gene nucleotide sequences of the studied strains with homologous sequences of typical strains of the families Comamonadaceae, Burkholdereaceae and Pseudomonadaceae, placed in GenBank, the BLAST program was used. Dendrograms of nucleotide sequence affinity were constructed by the Neighbor Joining method using a two-parameter Kimura’s model based on 100 replicates of the bootstrap assay. The comparison of the 16S rRNA genes nucleotide sequences of isolated strains and deposited sequences of this gene in members of the families Comamonadaceae, Burkholdereaceae and Pseudomonadaceae showed the highest similarity with the members of the family Comamonadaceae. According to the complex of molecular-genetic and physiological-biochemical characteristics, the strains # 46 and # 47 are classified to Comamonas testosteroni
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