Abstract
High-pressure protein X-ray crystallography (HPPX) is a unique method that provides high-resolution structural information under various pressure conditions including hydration waters at a molecular surface and internal cavities. Two important applications of HPPX are (1) studying the mechanism of pressure tolerance of proteins from several organisms living in deep seas and (2) observation of functional sub-states of biological macromolecules. After brief introduction, two examples of the recent advances are described in this article. The first one is the study on the mechanism of pressure tolerance of 3-isopropylmalate dehydrogenase from high-pressure adapted deep-sea organisms. The second example is the observation of pressure-induced conformational substate of hen egg white lysozyme, which reveals a part of the reaction mechanism of the enzyme.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: THE REVIEW OF HIGH PRESSURE SCIENCE AND TECHNOLOGY
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
