WS13.02 Comparison of qPCR and 16S rRNA marker-gene Next-Generation Sequencing (NGS) for the detection and quantification of respiratory pathogens in the sputum of people with cystic fibrosis (PwCF)

S Carson,P Arooj,A Marshall,J Carson,G.G Einarsson,K Deasy,Y Mccarthy,J Dorgan,M Mccarthy,C Flemming,T Vagg,J.A Eustace,D Murphy,D.F Gilpin,S.J Elborn,B.J Plant,M.M Tunney

doi:10.1016/s1569-1993(23)00260-6

WS13.02 Comparison of qPCR and 16S rRNA marker-gene Next-Generation Sequencing (NGS) for the detection and quantification of respiratory pathogens in the sputum of people with cystic fibrosis (PwCF)

S Carson, P Arooj + Show 15 more

https://doi.org/10.1016/s1569-1993(23)00260-6

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Journal: Journal of Cystic Fibrosis

Publication Date: Jun 1, 2023

Affiliation: Queen's University Belfast, University College Cork, Cork University Hospital

#Quantification Of Pathogens #People With Cystic Fibrosis #Comparison Of qPCR #Front Microbiol #Quantification Of Bacteria #Non-culture Methods #Google Scholar #Clin Microbiol #Next-Generation Sequencing #Detection Of Pathogens

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Objectives: Non-culture methods are increasingly used for the detection and quantification of pathogens [ 1 Forbes et al. Front Microbiol. 2017; 8: 1069 Crossref PubMed Scopus (177) Google Scholar ]. qPCR allows absolute quantification of target genes, while 16S rRNA marker-gene MiSeq sequencing (NGS) provides taxonomic resolution to a genera level [ 2 Janda JM Abbott SL J Clin Microbiol. 2007; 45: 2761-2764 Crossref PubMed Scopus (1194) Google Scholar ], with the relative abundance of each of these bacterial genera determined within a sample. The aim of this study was to compare qPCR and relative abundance of 16S rRNA amplicons for the detection and quantification of bacteria within sputum samples from PwCF.

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WS13.02 Comparison of qPCR and 16S rRNA marker-gene Next-Generation Sequencing (NGS) for the detection and quantification of respiratory pathogens in the sputum of people with cystic fibrosis (PwCF)