Wrażliwość grzybów drożdżopodobnych na olejek majerankowy (Oleum Majoranae)

Abstract

Introduction. Majoram possesses a number of therapeutic properties. Essential oil contain, following components: oxygenated monoterpenes (α-and β-pinene, camphene, α-phellandrene, α-, β-, and δ-terpinene, cymene), oxygenated monoterpenes (1,8-cineoe, cis-sabinene hydrate, linalool, terpinene-4-ol, α-tepineol, linalyl acetate), sesquiterpene hydrocarbons (cubebene, β-longpinene, β-caryophyllene, α-humulene, β-farnezene) and oxygenated sesquiterpenes (caryophyllene oxide). Majoran essential oil posses anti-inflammatory, antioxidant and antimicrobial activity. Aim. The aim of this work was investigate the susceptibility of yeastlike fungi to majoram oil. Material and methods. Yeastlike fungi were isolated from oral cavity of patients with candidosis. The strains of fungi belonging to the following genus: Candida albicans (12 strains), C. glabrata (2), C. guilliermondii (1), C. humicola (1), C. kefyr (2), C. krusei (4), C. lusitaniae (1), C. parapsilosis (4), C. tropicalis (5) and C. utilis (1) and 9 reference strains were tested. Investigation was carried out using the plate dilution method in Sabouraud’s agar. Inoculum contain 105 microorganisms per spot was seeded with Steers replicator upon surface of agar with and without essential oil (strain growth control). The dates of concentrations of majoran oil (Semifarm) was 2.0, 1.0, 0.5, 0.25 and 0.12 mg/ml. Inoculated plates were incubated in aerobic conditions at 37°C for 24-48 hrs. The MIC was defined as the lowest concentration of majoram oil inhibiting the visible growth of yeastlike fungi. Results. The results of the dates indicated that the strains from genus of Candida was susceptible to oil in concentration from 1.0 to ≥ 2.0 mg/ml. The growth of the strain Candida albicans, C. guilliermondii and C. humicola were inhibited in concentration 1.0 mg/ml. The strains of C. glabrata, C. kefyr, C. krusei, C. parapsilosis and C. tropicalis were less sensitive (MIC 1.0-≥ 2.0 mg/ml). However the strains of C. lusitaniae and C. utilis were the lowest sensitive. The growth of this yeastlike fungi was inhibited by concentrations ≥ 2.0 mg/ml. Conclusions. The tested yeastlike fungi were variability susceptible to majoram oil. The oil was the most active towards C. albicans, C. guilliermondii and C. humicola strains. Strains of C. lusitaniae and C. utilis were the lowest sensitive on majoram oil.