Wpływ ekstraktów z liści kaliny koralowej (Viburnum opulus L.) na wzrost ludzkich komórek jelita

Abstract

Introduction. Cranberrybush (Viburnum opulus L.) is a plant rich in phenols. Chlorogenic acid is a main compound responsible for its antioxidant activity. This plant also has antibacterial and anti-inflammatory properties, for this reason it is used for prevention, as well as for the treatment of many diseases, mainly those of the genitourinary tract, but also of the heart or lungs. So far, there are few studies that suggest V. opulus has anti-cancer activity and could become a supplement in such therapies. Aim. The aim of the study was to assess and compare the effect of both leaf extract (ELK) and phenol-rich leaf extract from cranberrybush (FELK) on the growth of colon cancer and normal colon cells. Material and methods. The HPLC method allowed to estimate the chemical composition of ELK and FELK. In addition, two colon cancer cell lines HT29 and SW480 were used, as well as normal epithelial cells CCD841CoN. The cells were treated with various concentrations of V. opulus extracts. The cell viability was assessed by MTT test after 24 h, 48 h and 72 h, respectively. Results. ELK and FELK extracts had moderate effect on the inhibition of HT29 cell growth. The cell viability of SW480 was much more pronounced after ELK and FELK treatment. The FELK extract inhibited the growth of HT29 and SW480 more significantly compared to ELK. The growth of CCD841CoN cells was higher after FELK treatment than ELK. Conclusions. The purified leaf extract of V. opulus (FELK) was richer in phenolic compounds than the unpurified ELK, more significantly inhibited the growth of HT29 and SW480 colon cancer cells as well as had a more gentle influence on the growth of normal epithelial CCD841CoN colon cells.