Wood Metabolomic Responses of Wild and Cultivated Grapevine to Infection with Neofusicoccum parvum, a Trunk Disease Pathogen.

Clément Labois,Hélène Laloue,Céline Tarnus,Kim Wilhelm,Christophe Bertsch,Mary-Lorène Goddard,Julie Chong

doi:10.3390/metabo10060232

Clément Labois, Hélène Laloue + Show 5 more

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https://doi.org/10.3390/metabo10060232

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Journal: Metabolites	Publication Date: Jun 4, 2020
Citations: 20	License type: CC BY 4.0

Affiliation: University of Upper Alsace, University of Strasbourg

Abstract

Grapevine trunk diseases (GTDs), which are associated with complex of xylem-inhabiting fungi, represent one of the major threats to vineyard sustainability currently. Botryosphaeria dieback, one of the major GTDs, is associated with wood colonization by Botryosphaeriaceae fungi, especially Neofusicoccum parvum. We used GC-MS and HPLC-MS to compare the wood metabolomic responses of the susceptible Vitis vinifera subsp. vinifera (V. v. subsp. vinifera) and the tolerant Vitis vinifera subsp. sylvestris (V. v. subsp. sylvestris) after artificial inoculation with Neofusicoccum parvum (N. parvum). N. parvum inoculation triggered major changes in both primary and specialized metabolites in the wood. In both subspecies, infection resulted in a strong decrease in sugars (fructose, glucose, sucrose), whereas sugar alcohol content (mannitol and arabitol) was enhanced. Concerning amino acids, N. parvum early infection triggered a decrease in aspartic acid, serine, and asparagine, and a strong increase in alanine and β-alanine. A trend for more intense primary metabolism alteration was observed in V. v. subsp. sylvestris compared to V. v. subsp. vinifera. N. parvum infection also triggered major changes in stilbene and flavonoid compounds. The content in resveratrol and several resveratrol oligomers increased in the wood of both subspecies after infection. Interestingly, we found a higher induction of resveratrol oligomer (putative E-miyabenol C, vitisin C, hopeaphenol, ampelopsin C) contents after wood inoculation in V. v. subsp. sylvestris.

Highlights

Grapevine (Vitis vinifera) is a very economically important crop worldwide but it is susceptible to a wide range of pathogens
Changes in Primary Metabolites in Wood upon N. parvum Inoculation and V. v. subsp. vinifera genotypes infected with N. parvum, we analyzed three biological replicates
At three days post-infection (DPI), we could measure a significant increase in different stilbenes in V. v. subsp. sylvestris and V. v. subsp. vinifera, especially transresveratrol, pallidol, trans- -viniferin, -viniferin, several resveratrol trimers, and tetramers including hopeaphenol

Summary

Introduction

Grapevine (Vitis vinifera) is a very economically important crop worldwide but it is susceptible to a wide range of pathogens. One of the major threats to vineyard sustainability is Grapevine trunk diseases (GTDs). The incidence of GTDs has increased considerably over the past few decades and the economic losses of these diseases were estimated to exceed 1 billion dollars per year [1]. GTDs are considered a major threat to winegrowers since no effective plant protection strategy is available [2]. GTDs are associated with the development of a complex of xylem-inhabiting fungi, leading to the death of the plant. These fungi are characterized by a latent phase, where they are present in the plant as endophytes and by a pathogenic phase, where they become virulent and colonize grapevine

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